ACARA VI. PENGAMATAN MORFOLOGI TELUR IKAN NILEM
4.2.3 Penyusutan Diameter Telur
Penyusutan diameter telur yang diawetkan dengan larutan formalin
dikarenakan kandungan terbesar dari formalin ialah alkohol. Alkohol merupakan larutan
dengan daya dehidrasi yang kuat dan menyebabkan pengerasan dan pengerutan jaringan.
Alkohol dapat mengkoagulasi protein dan presipitasi glukogen dan melarutkan lemak.
Sebagian besar di dinding sel mikroba yang terdapat pada lapisan luar telur dimiliki lemak,
sehingga alkohol dapat melarutkan lemak dalam konsentrasi tinggi (Silalahi, 2009). Hal ini
menyebabkan diameter telur ikan yang diawetkan dengan larutan formalin mengalami
penyusutan.
Perubahan yang terjadi pada diameter telur yang diawetkan dengan larutan
gilson tidak berbeda dengan pengawetan yang menggunakan larutan formalin, yaitu terjadi
penyusutan diameter telur. Hal ini terjadi karena kandungan terbesar dalam gilson sama
dengan kandungan yang terdapat pada larutan formalin yaitu alkohol (Silalahi, 2009).
Berdasarkan besar kecilnya persentase penyusutan diameter telur ikan,
pengawetan yang terbaik adalah formalin. Hal ini dikarenakan hasil akhir pengawetan
HAJI M, 2013 IKL UNSOED
hanya mengalami sedikit perubahan morfologi pada telur sehingga formalin yang lebih
baik dalam mempertahankan mrfologi telur awal.
V. KESIMPULAN DAN SARAN
5.1. Kesimpulan
1. Bentuk telur pada ikan nilem berbeda-berbeda berdasarkan perbedaan
pengawetan
2. Dari 10 sampe telur, terdapat 4 telur berbentuk oval, 2 berbentuk tak beraturan, 4
berbentuk bulat
3. Factor yang mempengaruhi ukuran telur ialah ukuran tubuh ikan, sifat parental
care, kondisi lingkungan dan nutrisi, mekanisme reabsorbsi telur, dan TKG.
4. Telur mengalami penyusutan setelah diawetkan (Larutan Gilson dan Formalin)
dan telur menjadi keras (Freezzer atau pendinginan)
5.2 Saran
Ketelitian seorang praktikan dalam melakukan praktikum pada setiap acara,
kerjasama antar praktikan dalam menyelesaikan setiap acara praktikum merupakan hal
utama yang perlu diperhatikan. Berhati-hati dalam menggunakan alat-alat laboratorium
harus sangat diperhatikan agar tidak terjadi hal-hal yang tidak di inginkan dan setiap
DAFTAR PUSTAKA
Ardias Nurdianti. 2008. Peranan Nacl Terhadap Derajat Pembuahan Penetasan Telur
Dan Kelangsungan Hidup Larva Ikan Koi ( Cyprinus Carpio). Skripsi.
Institut Pertanian Bogor.
Auldry F. Walukow, 2011. Condition The Biology Parameter Plankton And Fish
In Sentani Lake . Jurnal Bologi Indonesia 7(1) : 187-193(2011).
Bagenal, T.B. and E. Braum. 1968. Eggs and Early Life History, dalam W.E Ricker ed.
Methods For Assement of Fish Production In Fish Water. Blackwell Scientific
Publication
Bolle, Loes. 2009. Variability in transport of fish eggs and larvae. III . Effects of
hydrodynamics and larval behavior on recruitment in place. Marine
ecology progress series. Vol 390: 195-211.
Cholik, F et all., 2005. Akuakultur: Tumpuan Harapan Harapan Masa Depan
Bangsa. Masyarakat Perikanan Nusantara dan Taman Akuarium Air Tawar TMII.
Jakarta.
Costa W J.E.M & Fabiano Leal. 2009. Egg surface morphology in the neotropical
seasonal killifish genus leptolebias ( Teleostei : Aplocheiloidei : Rivulidae). Avertebrata zoology. Vol 59(25-29).
Djajadireja, R.R.S, hatimah dan Z.Arifin., 1997. Buku Pedoman Perikanan
Darat. bagian I (Jenis-jenis Ikan Ekonomis Penting). Departemen Pertanian.
Jakarta.
Effendi, M.I. 1979. Metode Biologi Perikanan. Yayaysan Dewi Sri, Bogor.
--- M.I. 1997. Biologi Perikanan. Kanisius. Yayasan Pustaka Nusatama, Jogjakarta.
--- M.I .2002. Biologi Perikanan. Yayasan Pustaka Nusutuma, Yogyakarta
Fahry unus dan sharifuddin bin andry omar. 20120. Analisis Fekunditas Dan
Diameter Telur Ikan Malaglugis Biru ( Decapterus Macarellus Cuvier, 1833) Di Perairan Kabupaten Banggai Kepulauan, Provinsi Sulawesi Tengah. Torani (
Jurnal Ilmu Kelautan Dan Perikanan). Vol. 20(1) : 37-43.
Fujaya, Y. 2004. Fisiologi Ikan. Dasar pengembangan Tehnik Perikanan. Rineka Cipta. Jakarta.
Hoar, W.S & D.J. Randall. 1969. Fish Physiology Vol. III. Academic Press.
Hadiaty, R.K. 2000. Bebeberapa Catatan Tentang Aspek Pertumbuhan, Makan
dan Reproduksi Ikan Nilem Paitan (Osteochillus jeruk Hadiaty & Siebert, 1998).
Berita Biologi 2 : 151-156 .
Isau, Ziara. 2011. Structural analysis of oocytes. Post-fertilization events and embryonic
development of the Brazilian endangered teleost brycon insignis (characiformes). Cambridge University ;vol 21:85-94.
Khairuman., K, Amri. 2008. Buku Pintar Budidaya 15 ikan konsumsi. Agro Media.Pustaka. Jakarta.
Khotimiyati, A. 2004. Aspek Biologi Reproduksi Ikan Brek (Puntius orphoides) di
sungai Klawing Kabupaten Purbalingga. Skripsi. Universitas Jenderal Soedirman, Purwokerto.
Linhart, O. Kudo, S. Billard, R. Slechta, V and Mikodina, E.V. 1995. Morphology
Composition and Fertilization of Crab Eggs a Review Aquaculture. 129 :
75-93.
Moyle, P.B & J.J Cech. 1988. Fishes. An Introduction to Ichtyologi. Second Edition. Prentice Hall. New Jersey
Nugraha Dimas, et all., 2012. Pengaruh Perbedaan Suhu Terhadap
Perkembangan Embrio, Daya tetas telur dan kecepatan penyerapan kuning Telur ikan Black gosht (Apteronotus albifrons) PADA skala laboratorium.
Jurnal Perikanan. Vol 23 : 33-55
Pratiwi, Rita Rostika dan Yayat Dhahiyat, 2011. Pengaruh Tingkat Pemberian
PakanTerhadaplaju Pertumbuhan Dan Deposisi Logam Berat Pada Ikan Nilem Karamba Jaring Apung Waduk Ir. H. Juanda. Jurnal Akuatika Volume II. Nomor
2(1-11).
Pulungan. 2005. Penuntun Praktikum Biologi Perikanan.
Laboratorium Biologi Perikanan Manajemen Sumberdaya Perairan.
Fakultas Perikanan dan Kelautan. Universitas Riau. Jambi
Saanin H. 1984. Taksonomi dan Kunci Identifikasi Ikan. Vol I dan I. Bina Cipta. Bandung. Hal 1508.
Saanin, H. 1968. Taksonomi dan kunci identifikasi ikan. cetakan I. bina cipta, Jakarta.
Silalahi, Marsudin. 2009. Pengaruh Beberapa Bahan Pengawet Nabati
Terhadap Nilai Haugh Unit, Berat dan Kualitas Telur Konsumsi Selama Penyimpanan. Seminar nasional litbang pertanian. 2009.
Subiyanto, Dimas nugraha, Mustofa Niti Supardjo, 2012. Pengaruh Perbedaan Suhu
Terhadap Perkembangan Embrio, Daya Tetas Telur Dan Kecepatan
Penyerapan Kuning Telur Ikan Black Ghost (Apteronotus albifrons) Pada Skala Laboratorium. Journal Of Management Of Aquatic Resources, Vol
1, Nomor 1(1-6)
LAMPIRAN JURNAL
Vertebrate Zoology 59 (1) 2009 25
25-29 ©Museum Fur Tierkunde Dresden, ISSN 1864-5755, 29.05.2009
Egg Surface Morphology in The Neotropical Seasonal Killifish genus Leptolebias (Teleostei : Aplocheiloidei : RIvulidae)
WILSON J. E. M. Costa & FABIANO LEAL
Laboratorio de Ictiologia Geral e Aplicada, Departemento, de Zoologia, Universidade Federal do Rio de Jaenerio, Coaxal Postal 68049, CEP 21944-970, Rio de Janeiro, Brazil
Received on July, 24, 2008 accepted on October 6, 2008
Published online atwww.vertebrate-zoology.deon May 15, 2009
ABSTRACT
The egg surface of four species of the South American genus Leptolebias is examined under scanning electron microscopy. Two distinct morphological patterns are described: surface reticulate, lumen punctate, in L.
marmoratus, L. aureoguttatus, and L. itanhaensis; and, surface insulate and not punctate, in L. citrinipinnis.
Both the punctate lumen and the insulate pattern are features firstly recorded for rivulids. Phylogenetic inferences about evolution of unique characters states found are not postulated by the absence of data on chorion morphology for two congeners possibly extinct (L. splendens and L. opalescens). Mushroom-like projections about regularly distributed on egg surface were found in all examined species.
Keyword
Killifishes, Seasonal fishes, Leptolebias, Egg Morphology, Chorion.
Marine Ecology Progress Series , Vol 390 :
183-194, 2009 (Published) 18
Variability in transport of fish eggs and
larvae. II. Effects of hydrodynamics on the
transport of Downs herring larvae
Mark Dickey-Collas1,*, Loes J. Bolle1, Jan K. L. van Beek2, Paul L. A. Erftemeijer2
1Wageningen IMARES - Institute for Marine Resources & Ecosystem Studies, PO Box 68, 1970 AB IJmuiden, The Netherlands
2DELTARES (formerly Delft Hydraulics), PO Box 177, 2600 MH Delft, The Netherlands
African Journal of Agricultural Research Vol. 7(10), pp. 1556-1561, 12 March, 2012 Available online at
http://www.academicjournals.or g/AJAR DOI:
10.5897/AJAR11.1483
ISSN 1991-637X ©2012 Academic Journals
Full Length Research Paper
Gut contents of Osteochilus hasselti
(Valenciennes,
1842) and Thynnichthys thynnoides
(Bleeker, 1852) from Kaeng Lawa, Khon
Kaen Province, Northeastern Thailand
Hanjavanit, C.* and Sangpradub,
N.
Department of Biology, Taxonomic Applied Research Center, Faculty of Science, Khon Kaen University,
Gut contents of the hard-lipped barb (Osteochilus hasselti (Valenciennes, 1842)) and the tiny scale barb (Thynnichthys thynnoides (Bleeker, 1852)) were analyzed.
Fish samples were collected from Kaeng Lawa, Khon Kaen Province,
northeastern Thailand during the hot season (April and May 2009) and the late
rainy season (September 2009). The percentage of frequency of occurrences
(%F) and percentage of proportions (%P) of food items were evaluated. O.
hasselti consumed 11 food items, only 7 categories were found in both seasons which consisted of detritus (66.67%, 42.00%P), plant parts (21.34%, 35.00%P), algae (4.00%, 9.00%P), unidentified items (5.34%, 4.00%P), protozoa (1.06%, 2.00%P), aquatic insect fragments (0.53%, 2.00%P) and diatoms (1.06%,
0.30%P), respectively. Whereas,T. thynnoides consumed 7 food items and only
5 food catergories were found in both seasons consisting of detritus (87.36%, 70.00%P), rotifers (7.37%, 6.00%P), unidentified items (2.11%, 3.00%P), plant parts (2.11%, 1.00%P) and aquatic insect fragments (1.05%, 3.00%P). From Chi-Square test for independence, it was found that %P of food items depended on season and fish species (p < 0.05). The result of present study, it was concluded thatO. hasseltiandT. thynnoidesare omnivores.
Key words:Gut content, Osteochilus hasselti,Thynnichthys thynnoides, Kaeng Lawa, northeastern Thailand.
Middle-East Journal of Scientific Research 10 (3): 332-341, 2011 ISSN 1990-9233
© IDOSI Publications, 2011
Structure and Phylogenetic of Gnrh Genes of Hard-lipped Barb (Osteochilus hasselti C.V)
1,2N.A. Prayogo,3G.E. Wijayanti,2,6Murwantoko,4M. Kawaichi and2,5P. Astuti
1Fisheries and Marine Science, Jenderal Soedirman University, Purwokerto Indonesia
2Post Graduate Programme of Biotechnology Gadjah Mada University, Yogyakarta, Indonesia
3Postgraduate Programme, Magister Biologi, Jenderal Soedirman University, Purwokerto, Indonesia
4Departemen of Biology, Nara Institute Science and Technology, Nara, Japan
5Faculty of Veterinary Science, Gadjah Mada University, Yogyakarta, Indonesia
6
Faculty of Agriculture Science, Gadjah Mada University, Yogyakarta, Indonesia
Abstract: The gonadotropin-releasing hormone is known and named for its role as the final common
signaling molecule used by the brain to regulate reproduction in all vertebrates. Two Genomic DNA GnRH and cDNAs of Hard-lipped barb carp, namely GnRH-II and GnRH-III, were firstly cloned from the brain reverse transcription- polymerase chain reaction (RT-PCR). The length of genomic DNA GnRH-II was 632 bp and cDNA GnRH-II was
253 bp, the length of genomic DNA GnRH-III was 486 bp and cDNA GnRH-II was 285 bp, respectively. The GnRH-II precursors encoded cDNA consisted of 84 amino acids and GnRH-III precursors encoded cDNA consisted of 95 amino acids, including a signal peptide, GnRH-II decapeptide and a GnRH-associated peptide (GAP) linked by a Gly-Lys-Arg proteolytic site. Recently, genes encoding two GnRH forms in hard-lipped barb have been discovered.
Key words: GnRH-II GnRH-III PCR Amino Acid