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27 Lampiran 1 Komposisi media Nutrient Agar, King’s B Agar, Yeast Manitol Agar,
dan Susu Skim + Molase yang digunakan dalam 100 ml aquades 1. Media Nutrient Agar (NA)
Nutrient Broth : 0.8 g Agar Bacto : 1.5 g
Aquades : 100 ml
pH : 6.8
2. Media King’s B Agar
Agar Bacto : 1.5 g Pepton : 2 g K2HPO4 : 0.15 g MgSO4 : 0.15 g Gliserol : 1.5 g Aquades : 100 ml pH : 7.2 ± 0.2
3. Media Yeast Manitol Agar (YMA) Agar Bacto : 2 g Mannitol : 1 g Yeast Ekstrak : 0.1 g K2HPO4 : 0.05 g MgSO4 : 0.02 g NaCl : 0.02 g Aquades : 100 ml pH : 6.8 ± 0.2
4. Media Susu Skim + Molase Susu Skim : 2 g K2HPO4 : 0.15 g MgSO4 : 0.15 g Molase : 1.5 ml Aquades : 100 ml pH : 7
28
Lampiran 2 Viabilitas sel bakteri pada paket dalam bentuk kemasan selama masa penyimpanan pada suhu ruang
Paket (Bentuk Kemasan)
Kode Isolat
Populasi Bakteri (Sel/g) pada suhu ruang bulan ke-
0 1.5 3 6 M_RS (Serbuk) Cr 76 1.00 x 109 7.00 x 108 2.60 x 108 4.75 x 107 Crb 86 6.50 x 109 9.00 x 108 4.00 x 106 6.65 x 107 BJ 11 5.00 x 108 1.45 x 108 6.00 x 108 1.66 x 106 M_RS (Granul) Cr 76 2.20 x 109 3.00 x 108 2.00 x 108 6.60 x 107 Crb 86 3.00 x 108 7.00 x 108 3.00 x 108 2.90 x 107 BJ 11 1.20 x 108` 1.50 x 108 1.30 x 105 2.14 x 106 M_RS (Tepung) Cr 76 2.20 x 109 3.50 x 108 5.00 x 108 4.91 x 107 Crb 86 8.15 x 109 3.00 x 108 3.00 x 107 6.50 x 107 BJ 11 1.50 x 108 1.00 x 107 3.50 x 105 1.15 x 106 M_SR (Serbuk) Cr 55 1.60 x 1010 1.40 x 108 1.70 x 107 3.84 x 108 Crb 64 3.00 x 109 4.00 x 108 2.90 x 107 3.50 x 107 BJ 11 6.70 x 109 6.50 x 108 1.60 x 108 5.25 x 106 M_SR (Granul) Cr 55 2.50 x 109 4.50 x 108 6.00 x 107 9.00 x 107 Crb 64 3.50 x 108 2.00 x 107 1.00 x 107 2.50 x 107 BJ 11 1.00 x 108 1.00 x 107 1.70 x 106 1.72 x 107 M_SR (Tepung) Cr 55 7.75 x 109 1.80 x 107 1.00 x 107 3.24 x 108 Crb 64 1.00 x 109 1.00 x 106 2.60 x 107 3.20 x 107 BJ 11 2.00 x 108 - 3.00 x 105 4.60 x 106 M_FO (Serbuk) Cr 24 1.55 x 109 3.40 x 108 3.00 x 108 1.33 x 108 Crb 17 8.50 x 107 6.00 x 108 2.00 x 108 1.06 x 108 BJ 11 2.50 x 109 1.50 x 108 7.00 x 108 4.40 x 107 M_FO (Granul) Cr 24 2.50 x 109 3.50 x 108 1.30 x 108 1.55 x 107 Crb 17 3.00 x 109 1.00 x 108 1.00 x 108 5.40 x 107 BJ 11 4.75 x 109 1.50 x 108 4.00 x 108 2.14 x 107 M_FO (Tepung) Cr 24 5.00 x 109 7.00 x 108 3.00 x 108 1.85 x 108 Crb 17 2.25 x 108 2.00 x 108 3.00 x 108 1.27 x 108 BJ 11 1.35 x 106 1.00 x 108 1.00 x 108 1.67 x 108 Keterangan:
29 Lampiran 3 Viabilitas sel bakteri pada paket dalam bentuk kemasan selama masa
penyimpanan pada suhu 4 °C
Paket (Bentuk Kemasan)
Kode Isolat
Populasi Bakteri (Sel/g) pada suhu ruang bulan ke-
0 1.5 3 6 M_RS (Serbuk) Cr 76 1.00 x 109 4.50 x108 4.00 x 108 1.35 x 107 Crb 86 6.50 x 109 3.50 x 108 1.40 x 108 1.15 x 108 BJ 11 5.00 x 108 1.15 x 108 1.30 x 108 1.66 x 106 M_RS (Granul) Cr 76 2.20 x 109 5.50 x 108 1.00 x 107 1.85 x 107 Crb 86 3.00 x 108 2.50 x 108 4.90 x 106 2.50 x 107 BJ 11 1.20 x 108` 2.00 x 107 1.00 x 106 2.14 x 106 M_RS (Tepung) Cr 76 2.20 x 109 6.00 x 108 9.00 x 108 6.40 x 107 Crb 86 8.15 x 109 2.00 x 108 2.50 x 106 3.66 x 107 BJ 11 1.50 x 108 2.50 x 106 7.00 x 105 4.60 x 106 M_SR (Serbuk) Cr 55 1.60 x 1010 7.00 x 108 2.00 x 108 6.10 x 107 Crb 64 3.00 x 109 3.50 x 108 4.00 x 108 3.80 x 107 BJ 11 6.70 x 109 1.00 x 108 7.00 x 108 1.18 x 107 M_SR (Granul) Cr 55 2.50 x 109 1.00 x 108 1.60 x 107 1.84 x 108 Crb 64 3.50 x 108 8.50 x 108 2.00 x 107 2.70 x 107 BJ 11 1.00 x 108 4.00 x 108 2.70 x 107 8.50 x 106 M_SR (Tepung) Cr 55 7.75 x 109 6.50 x 108 6.10 x 108 1.44 x 107 Crb 64 1.00 x 109 3.00 x 108 3.90 x 107 3.60 x 107 BJ 11 2.00 x 108 - - - M_FO (Serbuk) Cr 24 1.55 x 109 2.00 x 108 4.00 x 108 5.65 x 107 Crb 17 8.50 x 107 2.50 x 108 3.00 x 108 3.75 x 107 BJ 11 2.50 x 109 1.20 x 108 2.00 x 108 3.30 x 106 M_FO (Granul) Cr 24 2.50 x 109 9.50 x 108 5.10 x 108 1.70 x 107 Crb 17 3.00 x 109 2.00 x 108 1.00 x 108 2.00 x 107 BJ 11 4.75 x 109 9.00 x 108 2.00 x 108 3.70 x 107 M_FO (Tepung) Cr 24 5.00 x 109 1.40 x 108 6.00 x 108 7.80 x 107 Crb 17 2.25 x 108 3.00 x 108 1.00 x 108 6.80 x 107 BJ 11 1.35 x 106 3.00 x 108 4.40 x 108 1.82 x 108 Keterangan: