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24
25 Lampiran 1 Keragaan asam lemak minyak ikan lemuru (Sardinella sp.)
Jenis Asam Lemak
Asam Lemak Minyak Ikan (%) Asam Laurat, C12:0 0,08±0,00 Asam Tridekanoat, C13:0 0,09±0,00 Asam Miristat, C14:0 4,38±0,02 Asam Pentadekanoat, C15:0 0,91±0,00 Asam Palmitat, C16:0 15,62±0,01 Asam Heptadekanoat, C17:0 0,88±0,00 Asam Stearat, C18:0 3,88±0,00 Asam Arakidat, C20:0 0,50±0,01 Asam Heneikosanoat, C21:0 0,10±0,01 Asam Behenat, C22:0 0,19±0,00 Asam Trikosanoat, C23:0 0,07±0,00 Asam Lignoserat, C24:0 0,13±0,00 Σ SFA 26,41±0,48 Asam Miristoleat, C14:1 0,02±0,00 Asam Palmitoleat, C16:1 4,45±0,07 Asam Cis-10-Heptadekanoat, C17:1 0,30±0,01 Asam Elaidat, C18:1n9t 0,45±0,07 Asam Oleat, C18:1n9c 9,76±0,19 Asam Cis-11-Eikosenoat C20:1 0,83±0,03 Asam Erusat, C22:1n9 0,23±0,03 Asam Nervonat, C24:1 0,37±0,00 Σ MUFA 16,44±0,69 Asam Linoleat, C18:2n6c 1,27±0,02 Asam ϒ-Linolenat, C18:3n6 0,12±0,00 Asam Linolenat, C18:3n3 0,89±0,00 Asam Cis-11,14-Eikosedienoat C20:2 0,29±0,00 Asam Cis-8,11,14-Eikosetrienoat, C20:3n6 0,11±0,00 Asam Cis-11,14,17-Eikosetrienoat C20:3n3 0,07±0,00 Asam Arakhidonat, C20:4n6 1,58±0,02 Asam Cis-5,8,11,14,17-Eikosapentaenoat, C20:5n3 8,03±0,04 Asam Cis-4,7,10,13,16,19-Dokosaheksaenoat, C22:6n3 17,48±0,13 Asam Cis-13, 16-Docosadienoic Acid, C22:2 0,03±0,00
26
Lampiran 2 Hasil uji FTIR dan interpretasi spektrum IR serbuk kitosan
Vibrasi Stretching Vibrasi Bonding Rentang (cm-1) Intensitas Ikatan Tipe Senyawa Rentang (cm-1) Intensitas Ikatan Tipe Senyawa 3780 medium O-H alkohol 1427 medium O-H asam
karboksilat 3448 medium N-H amina 1381 medium O-H fenol 2924 lemah O-H alkohol 895 kuat C=C alkena
1643 kuat C=C alkena
27 Lampiran 3 Hasil uji FTIR dan interpretasi spektrum IR film PVA 8%+kitosan
2%+gliserol
Vibrasi Stretching Vibrasi Bonding
Rentang
(cm-1) Intensitas Ikatan Tipe Senyawa
Rentang
(cm-1) Intensitas Ikatan
Tipe Senyawa 2909 kuat N-H garam amina 1437 medium O-H
asam karboksilat 1720 kuat C=O
conjugated
anhydride 1335 medium O-H fenol 1681 kuat C=O conjugated acid
1085 medium C-N amina
28
Lampiran 4 Grafik hasil uji mekanik film PVA 8%+kitosan 2%+gliserol
Lampiran 5 Grafik hasil uji mekanik film PVA 8% (kontrol)
0 50 100 150 0 10 20 30 40 50 Strain in % F o rc e in k g 0 50 100 150 0 10 20 30 40 50 Strain in % F or ce in k g
29 Lampiran 6 Grafik pertambahan bobot pada pengujian laju transmisi uap air
Lampiran 7 Tabel hasil uji normalitas dan independent sampel t test bilangan iod minyak ikan lemuru selama penyimpanan
90,53 ± 5,57 90,84 ± 5,59 91,05 ± 5,6 91,32 ± 5,61 91,63 ± 5,63 93,09 ± 2,53 93,10 ± 2,53 93,11 ± 2,52 93,13 ± 2,52 93,17 ± 2,52 89.00 89.50 90.00 90.50 91.00 91.50 92.00 92.50 93.00 93.50
Hari Ke-1 Hari Ke-2 Hari Ke-3 Hari Ke-4 Hari ke-5
P er ta m ba ha n bo bo t sa m pel (g ra m ) PVA 8%+Kitosan 2%+Gliserol PVA 8%
One-Sample Kolmogorov-Smirnov Test
4 145,7800 2,73322 ,311 ,238 -,311 ,622 ,834 N Mean St d. Dev iation Normal Parametersa,b
Absolute Positiv e Negativ e Most Ext reme
Dif f erences
Kolmogorov -Smirnov Z Asy mp. Sig. (2-tailed)
iod
Test distribution is Normal. a.
Calculated f rom dat a. b. Group Statisti cs 2 147,5600 ,24042 ,17000 2 144,0000 3,11127 2,20000 Perlakuan PVA 8%+Chitosan 2%+Gliserol PVA 8% iod
N Mean St d. Dev iation
St d. Error Mean
30
Lampiran 8 Tabel hasil uji normalitas dan independent sampel t test bilangan peroksida minyak ikan lemuru selama penyimpanan
Independent Samples Test
2,863E+016 ,000 1,613 1,613 2 1,012 ,248 ,351 3,56000 3,56000 2,20656 2,20656 -5,93405 -23,70709 13,05405 30,82709 F Sig. Levene's Test f or Equality of Variances t df Sig. (2-tailed) Mean Dif ference Std. Error Dif ference
Lower Upper 95% Confidence Interval of the Dif f erence t-test for Equality of
Means Equal v ariances assumed Equal v ariances not assumed iod
One-Sample Kol mogorov-Smirnov Test
4 41,1000 1,30894 ,311 ,222 -,311 ,621 ,835 N Mean St d. Dev iation Normal Paramet ersa,b
Absolute Positiv e Negativ e Most Extreme Dif f erences Kolmogorov -Smirnov Z Asy mp. Sig. (2-tailed)
Peroksida
Test distribution is Normal. a.
Calculated f rom data. b. Group Statistics 2 41,9500 ,21213 ,15000 2 40,2500 1,48492 1,05000 Perlakuan PVA 8%+Chitosan 2%+Gliserol PVA 8% Peroksida
N Mean Std. Dev iation
Std. Error Mean
31
Lampiran 9 Tabel hasil uji normalitas dan independent sampel t test angka asam lemak bebas minyak ikan lemuru selama penyimpanan
Independent Samples Test
7,776E+016 ,000 1,603 1,603 2 1,041 ,250 ,348 1,70000 1,70000 1,06066 1,06066 -2,86365 -10,59046 6,26365 13,99046 F Sig. Levene's Test f or Equality of Variances t df Sig. (2-tailed) Mean Dif ference Std. Error Dif ference
Lower Upper 95% Confidence Interval of the Dif f erence t-test for Equality of
Means Equal v ariances assumed Equal v ariances not assumed Peroksida
One-Sample Kolmogorov-Smirnov Test
4 ,8225 ,33049 ,325 ,325 -,175 ,650 ,792 N Mean St d. Dev iation Normal Parametersa,b
Absolute Positiv e Negativ e Most Ext reme
Dif f erences
Kolmogorov -Smirnov Z Asy mp. Sig. (2-tailed)
FFA
Test distribution is Normal. a.
Calculated f rom dat a. b. Group Statisti cs 2 ,6250 ,19092 ,13500 2 1,0200 ,36770 ,26000 Perlakuan PVA 8%+Chitosan 2%+Gliserol PVA 8% FFA
N Mean St d. Dev iation
St d. Error Mean
32
Independent Samples Test
5,823E+015 ,000 -1,348 -1,348 2 1,503 ,310 ,345 -,39500 -,39500 ,29296 ,29296 -1,65550 -2,15326 ,86550 1,36326 F Sig. Levene's Test f or Equality of Variances t df Sig. (2-tailed) Mean Dif ference Std. Error Dif ference
Lower Upper 95% Confidence Interval of the Dif f erence t-test for Equality of
Means FFA Equal v ariances assumed Equal v ariances not assumed
33
RIWAYAT HIDUP
Penulis dilahirkan di Bekasi, Jawa Barat pada tanggal 2 November 1992. Penulis merupakan anak pertama dari pasangan Muhamad Taufik dan Reny Ambarwati. Pendidikan formal yang ditempuh penulis dimulai dari SDN Cilincing 05 Pagi, Jakarta Utara pada tahun 1998 hingga tahun 2004. Kemudian penulis melanjutkan pendidikan pada tahun 2004 hingga tahun 2007 di SMPN 244 Jakarta Utara dan dilanjutkan dengan pendidikan formal di SMAN 75 Jakarta Utara dari tahun 2007 hingga tahun 2010.
Penulis diterima sebagai mahasiswa pada Departemen Teknologi Hasil Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor melalui jalur USMI (Undangan Seleksi Masuk IPB). Penulis aktif sebagai asisten mata kuliah Teknologi Pengolahan Hasil Perairan periode 2013/2014. Penulis juga pernah aktif mengikuti lomba karya tulis ilmiah PKM-Penelitian 2013 yang didanai oleh DIKTI yang berjudul “Model Baru Advanced Packaging melalui Mekanisme Aktif Oxygen Scavenging dari Chitosan untuk Kemasan Suplemen Pangan Kecerdasan berbasis Asam Lemak Omega-3 Minyak Ikan”. Selain itu penulis juga pernah melakukan praktek lapangan di perusahan perikanan dengan
judul “Penerapan Kelayakan Dasar pada Pembekuan Tuna Loin di PT Makmur
Jaya Sejahtera, Muara Baru, Jakarta Utara”. Sebagai syarat untuk memperoleh gelar sarjana pada program studi Teknologi Hasil Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor, penulis menyusun skripsi dengan
judul “Kemasan Aktif Minyak Ikan dengan Material Komposit Kitosan”.