Pengukuran nilai pH setelah proses pembuatan larutan ASC sebaiknya dilakukan sebelum penggunaan sebagai sanitaiser. Hal ini berguna untuk mengetahui pH standar yang disyaratkan oleh Food Drug Administration yaitu sekitar 2,3 -3,2. Pengukuran konsentrasi larutan setelah pencelupan dan pengukuran residu Acidified Sodium Chlorite
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Lampiran 1a. Hasil Pengujian Sanitaiser terhadap Jumlah Escherichia coli (log) Perlakuan Konsentrasi
(ppm)
Jumlah E.coli (log)
Rata-rata Ulangan 1 Ulangan 2
Sebelum Perlakuan - 6.367729 5.547775 5,957752 Setelah perlakuan
Air - 5.576341 4.826075 5,201208
Acidified Sodium Chlorite (ASC) 80 4.755875 4.716003 4,735939 90 4.832509 4.278754 4,555631 100 4.342423 4.431364 4,386893 Sodium hipoklorit 80 4.973128 4.755875 4,864501 90 4.69897 4.342423 4,520696 100 4.491362 4.477121 4,484241
Lampiran 1b. Perubahan pH dan Suhu pada Uji Efektivitas Sanitaiser terhadap
Escherichia coli
Perlakuan Konsentrasi
(ppm) Ulangan
Perubahan pH Perubahan suhu
(0C)
Awal Akhir Awal Akhir
Air 1 9.0 8.71 -0.2 0.1 2 7.53 7.48 -0.2 0.2 ASC 80 1 7.84 7.77 -0.3 0.1 80 2 6.32 6.55 -0.3 0.2 90 1 7.62 7.76 -0.3 0.2 90 2 5.82 6.37 -0.2 0.5 100 1 7.26 7.56 -0.3 0.1 100 2 6.15 6.06 -0.2 0.3 Sodium hipoklorit 80 1 8.45 8.38 -0.3 0.1 80 2 8.50 7.48 -0.3 0.3 90 1 9.24 8.45 -0.2 0.1 90 2 8.48 7.32 -0.2 0.3 100 1 9.46 8.43 -0.2 0.1 100 2 8.46 7.45 -0.2 0.1
Lampiran 2a. UjiANOVA dengan Metode General Linier Model Source Type III
Sum of Squares df Mean Square F Sig. Corrected Model 0,362(a) 5 0,072 2,425 0,156
Intercept 255,853 1 255,853 8562,108 0,000 Sani 0,036 1 0,036 1,200 0,315 Kons 0,319 2 0,160 5,343 0,046 sani* kons 0,007 2 0,004 0,120 0,889 Error 0,179 6 0,030 Total 256,395 12 Corrected Total 0,542 11
Dependent Variable: Reduksi E.coli (log)
Pengaruh antar konsentrasi menunjukkan perbedaan nyata (p=0.046 < 0.05)
Lampiran 2b. Uji Lanjut dengan Metode Tukey-LSD untuk Melihat Interaksi Antar Konsentrasi (I) kons (J) kons Mean Differenc e (I-J) Std. Error Sig.(a) 95% Confidence Interval for Difference(a) Lower Bound Upper Bound 80,00 90,00 ,250 ,122 ,087 -,049 ,549 100,00 ,395(*) ,122 ,018 ,096 ,694 90,00 80,00 -,250 ,122 ,087 -,549 ,049 100,00 ,145 ,122 ,279 -,154 ,444 100,00 80,00 -,395(*) ,122 ,018 -,694 -,096 90,00 -,145 ,122 ,279 -,444 ,154
Dependent Variable: Reduksi E.coli (log) Based on estimated marginal means
* The mean difference is significant at the ,05 level.
a Adjustment for multiple comparisons: Least Significant Difference (equivalent to no adjustments).
Pengaruh konsentrasi 80 dan 100 ppm berbeda nyata (p=0.018) Pengaruh konsentrasi 80 dan 90 ppm tidak berbeda nyata (p=0.087) Pengaruh konsentrasi 90 dan 100 ppm tidak berbeda nyata (p=0.279)
Lampiran 2c. Uji Lanjut dengan Metode Tukey LSD untuk Melihat Interaksi Antar Bahan Sanitaiser
(I) fak (J) fak Mean Difference (I-J) Std. Error Sig.(a) 95% Confidence Interval for Difference(a) Lower Bound Upper Bound AQ5 Klorin -,109 ,100 ,315 -,354 ,135 Klorin AQ5 ,109 ,100 ,315 -,135 ,354
Based on estimated marginal means
a Adjustment for multiple comparisons: Least Significant Difference (equivalent to no adjustments).
Dependent Variable: Jumlah Reduksi E.coli (log)
Lampiran 3a. Hasil Pencelupan Udang terhadap Jumlah Total Mikroba (Reduksi log)
Ulangan Air ASC
Pencelupan 1 Pencelupan 3 Pencelupan 1 Pencelupan 3
1 0.719173 0.762639 0.831596 0.722451
2 1.0 0.837273 1.009697568 1.038661264
Rata-rata 0.859587 0.799956 0.920647 0.880056
Lampiran 3b. Uji Analysis of Variance antara ASC dan Air
Source Type III Sum of Squares df Mean Square F Sig. Corrected Model 4.977E-03 1 4.977E-03 3.834 0.189
Intercept 2.993 1 2.993 2305.9960.000
ASC*air 4.977E-03 1 4.977E-03 3.834 0.189
Error 2.596E-03 2 1.298E-03
Total 3.001 4
Corrected Total 7.574E-03 3
Lampiran 4a. Jumlah Escherichia coli Selama Pertumbuhan 16 Jam Jam ke OD Jumlah Bakteri Jumlah bakteri x 108
0 0.008 7800000 0.078 1 0.014 1690000 0.016 2 0.036 15600000 0.15 3 0.096 26000000 0.26 4 0.201 51000000 0.51 5 0.35 88000000 0.88 6 0.507 126000000 1.26 7 0.66 163000000 1.63 8 0.696 172000000 1.72 9 0.694 171000000 1.71 10 0.72 178000000 1.78 11 0.73 180000000 1.8 12 0.74 183000000 1.83 13 0.74 180000000 1.8 14 0.68 168000000 1.68 15 0.68 168000000 1.68 16 0.68 160000000 1.6
Lampiran 4b. Kurva Pertumbuhan Escherichia coli Laboratorium Food Processing PT. Centralpertiwi Bahari 0 0.2 0.4 0.6 0.8 1 1.2 1.4 1.6 1.8 2 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16
Waktu inkubasi (jam)
Ju m la h b a kt er i ( c fu /m l) x 10 8