BAB 4 HASIL DAN PEMBAHASAN
5.2. Saran
5.1.Simpulan
Berdasarkan penelitian didapatkan adanya penurunan jumlah spermatozoa yang bermakna pada kelompok mencit yang diinduksi gentamisin (K2) dibandingkan kelompok normal (K1) dan didapatkan perbaikan jumlah spermatozoa pada kelompok mencit yang diberikan gentamisin dan ekstrak jintan hitam (K3). Hal ini menunjukkan bahwa gentamisin 5 mg/kgbb i.p mampu menurunkan jumlah spermatozoa sehingga dapat digunakan sebagai model infertilitas pada hewan coba dan pemberian ekstrak biji jintan hitam (Nigella sativa) mampu meningkatkan jumlah spermatozoa mencit yang diinduksi gentamisin sebesar 85,2%.
5.2.Saran
Saran untuk penelitian berikutnya, antara lain:
1. Dilakukan penelitian mengenai efek ekstrak biji jintan hitam (Nigella sativa) terhadap jumlah spermatozoa secara in vivo
2. Dilakukan penelitian dengan parameter sperma yang lebih bervariasi baik secara makroskopik maupun mikroskopik
3. Dilakukan penelitian menggunakan dosis ekstrak biji jintan hitam (Nigella sativa) yang bervariasi untuk mengetahui dosis terendah dan tertinggi yang memiliki efek sebagai anti infertilitas
4. Dilakukan penelitian dengan waktu pemberian ekstrak biji Nigella sativa yang lebih bervariasi untuk mengetahui waktu minimal yang dibutuhkan untuk memberikan pengaruh anti infertilitas
5. Dilakukan penelitian menggunakan metode pelarutan dari biji jintan hitam (Nigella sativa) lainnya seperti minyak atau ekstrak metanol
6. Dilakukan pengujian ekstrak biji jintan hitam (Nigella sativa) untuk mengetahui fungsinya pada organ tubuh lain
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Surat Keterangan Sehat Mencit
Lampiran 2 Surat Determinasi Tanaman Nigella sativa
Lampiran 3 Bukti Pembelian Etanol 70%
Lampiran 4 Proses Penelitian 1. Ekstraksi Biji Jintan Hitam (Nigella sativa)
Gambar 6.4 Proses pencucian biji jintan hitam
(Nigella sativa)
Gambar 6.5. Proses penjemuran biji jintanhitam (Nigella sativa)
Gambar 6.6.Proses penghalusan biji jintan hitam
(Nigella sativa)
Gambar 6.8. Proses filtrasi Gambar 6.9. Filtrat mengandung pelarut
Gambar 6.10. Proses evaporasi
Gambar 6.11. Ekstrak kental biji jintan hitam (Nigella sativa)
2. Pengujian terhadap Hewan Coba
Gambar 6.12. Adaptasi hewan sampel (D 0-7)
Gambar 6.13 Pengukuran BB awal (D 8)
Gambar 6.14 Induksi gentamisin 5mg/kgbb.pada K2
dan K3 (D 8-D17)
Gambar 6.15 Pemberian ekstrak Nigella sativa1 g/kgbb
i.p pada K3(D18-38)
Gambar 6.16. Proses Terminasi (D-39)
Gambar 6.17 Pengambilan vesikula Seminalis mencit
Gambar 6.18 Pengeluaran cairan dari vesikula seminalis
mencit
Gambar 6.19 Sperma dilarutkan dalam larutan george
Gambar 6.20 Sperma diamati di bawah mikroskop
Lampiran 5 Cara perhitungan 1. Induksi gentamisin Sediaan : Vial 2 ml Konsentrasi 40 mg/ml Pengenceran M1V1 = M2V2 40 mg.1 ml = 1 mg. V2 V2 = 40 ml (Pengenceran 40 kali) 1 ml gentamisin + 3 ml aquabides = 40 ml Konsentrasi akhir = 40 mg/40 ml 1 mg/ml 2. Pemberian ekstrak
Dosis ekstrak jintan hitam yang diberikan adalah 1 gram/kgBB = 1000 mg/1000 gram
= 10 mg/10 gram
Konsentrasi ekstrak yang dihasilkan : 1 gram/1ml
Konsentrasi ekstrak yang diberikan : 10 mg/0,1ml
Sehingga 1000 mg ekstrak dilarutkan hingga mencapai 10 ml
Dosis Pemberian
10 mg/0,1 x 30 gram = 30 mg/x = 0,3 ml
Jadi untuk mencit dengan berat badan 30 gram dibutuhkan 0,3 ml ekstrak biji jintan hitam
Lampiran 6 Riwayat Penulis
Identitas
Nama : Hania Asmarani Rahmanita Jenis kelamin : Perempuan
Tempat, Tanggal Lahir : Jakarta, 20 April 1993
Agama : Islam
Alamat : Jalan Taman Agave 1 blok M2/3 RT 006 RW 014 Grand Galaxy City, Bekasi Selatan 17147
Email : hania.asmarani@hotmail.com
Riwayat Pendidikan
1999 - 2005 : SD Islam Darussalam Bekasi
2005 - 2008 : SMP Islam Al Azhar 6 Jakapermai
2008 – 2011 : SMAN 1 Bekasi
2011 – sekarang : Program Studi Pendidikan Dokter FKIK UIN Syarif Hidayatullah Jakarta
