BAB VI KESIMPULAN DAN SARAN
6.2 Saran
32 BAB VI
KESIMPULAN DAN SARAN
Dari hasil percobaan yang telah dilakukan dapat diambil beberapa kesimpulan dan saran saran untuk tindak lanjut penelitian berikutnya.
6.1 Kesimpulan
1. Rhamnolipid dan PHA dapat diproduksi dari bakteri P.aerugionsa dengan substrat dari produk samping industri biodiesel berbahan baku CPO
2. Konsetrasi awal sumber karbon (minyak sawit) 5 g/l memberikan hasil sel dan rhamnolipid terbaik dari beberapa konsetrasi yang telah diuji, sedangkan konsetrasi awal sumber karbon (minyak sawit) 10 g/l memberikan hasil sel dan PHA terbaik dari beberapa konsetrasi yang telah diuji pada kultivasi curah berulang secara simultan.
3. Konsetrasi awal sumber karbon (limbah biodiesel dari CPO) 25 g/l memberikan hasil sel, rhamnolipid dan PHA terbaik dari beberapa konsetrasi yang telah diuji, pada kultivasi curah berulang secara simultan.
4. Teknik kultivasi umpan curah dengan metode pengumpanan sumber C (produk samping industri biodiesel dari bahan baku CPO) secara impuls dapat meningkatkan produksi rhamnolipid; dan hasil maksimum sebesar 270 mg/l dicapai pada konsentrasi sumber karbon awal 5 g/l, selama 34 jam kultivasi. 5. Pada konsentrasi sumber karbon limbah cair industri biodiesel 25 g/l dengan
teknik kultivasi umpan curah berulang pengumpanan secara impuls diperoleh konsentrasi PHA dan berat kering sel yang lebih tinggi dibandingkan konsentrasi sumber karbon lain yang diuji yaitu masing masing secara berurutan adalah 0,35 g/l dan 1,2 g/l
6.2 Saran
Sebagai tindak lanjut penelitian ini disaran kan beberapa hal berikut ini terkait dengan uapaya untuk kelayakan produksi baik secara teknis maupun ekonomis.
33
1. Peningkatan kapasitas produksi/scale up ke tahap semi pilot.
2. Produksi PHA dan ramnolipid dengan pengumpanan secara kontinyu. 3. Identifikasi dan karakterisasi PHA dan ramnolipid yang dihasilkan. 4. Perhitungan kelayakan teknis dan ekonomis untuk produksi ramnolipid
sebagai bioinsektisida atau untuk keperluan sebagai bahan suplemen kosmetik
34
Daftar Pustaka
Ashby, R.D. dan T.A. Foglia. 1998. Poly(hydroxyalkanoates) biosynthesis from triglyceride substrate. Appl Microbil Biotechnol. 49: 431-437.
Chen G.Q., G Zhang, S.J. Park, dan S.Y. Lee, 2001, Industrial scale production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate), Appl Microbiol Biotechnol. 57: 50-55
Costa S.G.V.A.O, • Lépine F., •Milot S., Déziel E., •dan M. Nitschke, 2009, •Cassava wastewater as a substrate for the simultaneous production of rhamnolipids and polyhydroxyalkanoates by Pseudomonas aeruginosa, J
Ind Microbiol Biotechnol 36:1063–1072.
Davis R., P.K. Anil Kumar, A. Chandrasekar, dan T.R. Shamala, 2008, Biosynthesis of polyhydroxyalkanoates co-polymer in E.coli using genes from Pseudomonas and Bacillus, Antonie van Leeuwenhoek 94: 207-216 De Koning, G.J.M, dan B. Witholt, 1997, A process for recovery of
poly(hydroxyalkanoates) from Pseudomonads, prt 1: Solubilization. Bioprocess Engineering, 17, 7-13.
Hori, K., S. Marsudi, dan H. Unno. 2002. Simultaneous production of polyhydroxyalkanoates and rhamnolipid by Psedomonas aeruginosa.
Biotechnology and Bioengineering. 78 (6). 699-707.
Koch, A.K., O. Kappeli, A. Ficher, dan J. Reiser. 1991. Hydrocarbon assimilation and biosurfactant production in Pseudomonas aeruginosa mutants. Journal of Bacteriology.173 (13): 4212-4219.
Kronemberger F. A., L.M.M.S. Anna, A. C. L. B. Fernades, R. R. Menezes, C.P. Borges, dan D.M.G. Freire, 2008, Oxygen-controlled Biosurfactant Production in a bench Scale bioreactor, Appl Biochem Biotechnol 147:33-45
Lang, S. dan D. Wullbrant. 1999. Rhamnose lipids-biosynthesis, microbial production and application potential. Appl Microbiol Biotechnol. 51: 22-32.
Lee, S. Y., 1996a, Plastic bacteria? progress and prospects for polyhydroxyalkanoate production in bacteria. Trends Biotechnology, 14, 431-438
Lee, S. Y., 1996b, Bacterial polyhydroxyalkanoates, Biotechnology and
Bioengineering, 49, 1-14
Lee, L., S.Y. Lee, dan J.W. Yang. 1999. Production of rhmanolipid Biosurfactant by fed-batch culture of Pseudomonas aeruginosa using glucose as a sole carbon source. Biosci. Biotechnol. Biochem. 63(5): 946-947.
Lee, S.Y., H.H Wong, J. Choi, S.H. Lee, S.C. Lee, dan C.S. Han, 2000, Production of medium chain length polyhydroxyalkanoates by high-cell-density cultivation of Pseudomonas putida under phosphorus limitation.
Biotechnology and Bioengineering, 68(4): 466-470.
Lee K.M., S.H. Hwang, S.D. Ha, J. H. Jang, D.J. Lim, dan J. Y. Kong, 2004, Rhamnolipid production in batch and fed batch fermentation using pseudomonas aeruginosa BYK-2 KCTC 18012P, Biotechnology and Bioprocess engineering
35 Maier R. M. dan G. Soberon-Chavez. 2000. Pseudomonas aeruginosa
rhamnolipids: Biosynthesis and potential applications. Appl Microbiol
Biotechnol. 54: 625-633.
Matsufuji, M. K. Nakata, dan K. Yoshimoto, 1997. High Production of rhamnolipids by Pseudomonas aeruginosa growing on ethanol.
Biotechnol. Lett 19 : 1213 – 1215.
Marsudi, S. dan T. Setiadi, 1997, Preliminary study of polyhydroxyalkanoates (PHAs) biosynthesis by Rhadobacter sphaeroides IFO 12203 photosynthetic bacterium using volatile fatty acid as carbon sources, dalam
Proceeding of the Indonesian Biotechnology Conference (IBC-1997), June
17-19, Jakarta, Indonesia.
Marsudi, S., A. Ishihara, S. Yamamoto, dan H. Unno, 1998, Accumulation of polyhydroxyalkanoates (PHAs) by Alcaligenes eutrophus H16 utilizing carbon dioxide and valeric acid as carbon sources, dalam Proceeding of
Regional Symposium on Chemical Engineering, October 14-16, 1998,
Manila, Philippines, 241-246.
Marsudi S., 2002, Simultaneous Production of Polyhydroxyalkanoates (PHAs) and Rhamnolipids by Pseudomonas aeruginosa IFO 3924, Disertasi S3, Tokyo Institut Teknologi Indonesia, Tokyo Jepang.
Marsudi, S. 2002, Studi on simultaneous microbial production of polyhydroxyalkanoates and rhamnolipids. Disertasi S3, TIT, Tokyo, Jepang.
Marsudi, S., Irene K.P. Tan, S. N. Gan, dan K. B. Ramachandran, 2003, Production of Medium Chain Length Polyhydroxyalkanoates (PHAmcl) in Fermenter using saponified crude palm oil (SCPO) and saponified crude palm kernel oil (SCPKO) as Carbon sources, dalam Proceeding of the
International Conference on Chemical and Bioprocess Engineering
(ICCBPE 2003), August 27-29, 2003, Kota Kinibalu, Malaysia, 194-198 Marsudi, S., Irene K.P. Tan, S. N. Gan, dan K. B. Ramachandran, 2007,
Production of medium-chain-length Polyhydroxyalkanoates (PHA-mcl) from oleic acid using Pseudomonas Putida PGA1 by fed batch culture,
Makara Seri Teknologi, 11 (1): 1-6
Marsudi S., H. Unno dan K. Hori, 2008, Palm oil utilization for the simultaneous production of polyhydroxyalkanoates and rhamnolipids by Pseudomonas
aeruginosa, Appl. Microbiol Biotechnol 78: 955-961
Muller M.M., B. Hormann, C. Syldatk, dan R. Hausmann, 2010, Pseudomonas
aeruginosa PAO1 as a model for rhamnolipid production in bioreactor
systems, Appl Microbiol Biotechnol 83: 123-130
Neto D.C. , J.A. Meira, J.M. Araujo, D.A. Mitchell, dan N. Krieger, 2008, Optimization of the production of rhamnolipids by Pseudomonas
aeruginosa UFPEDA 614 in Solid-state culture, Appl Microbiol Biotechnol 81: 441-448
Serafim L.S., P.C.Lemos, M.G.E. Albuqueque, dan M.A.M. Reis, 2008, Strategies for PHA production by mixed cultures and renewable waste materials,
Appl Microbiol Biotechnol, 81:615-628
Sun Z., J.A. Ramsay, M. Guay, dan B.A. Ramsay, 2009, Fed batch production of unsaturated medium chain length polyhydroxyalkanoates with controlled
36 composition by Pseudomonas putida KT 2440, Appl Microbiol Biotechnol 82:657-662
Solaiman D.K.Y., R. D. Ashby, dan T.A. Foglia. 2001. Production of polyhydroxyalkanoates from intact triacylglycerols by genetically engineered Pseudomonas. Appl Microbiol Biotechnol. 56. 664-669.
37 LAMPIRAN
Lampiran 1a. Komposisi media IFO 802
Uraian Konsentrasi per liter air
Polypepton 10 g
Yeast Extract 2 g
MgSO4 7H2O 1 g
Lampiran 1b. Komposisi media garam dasar (Basssal Salt Medium, BSM) Uraian Konsentrasi per liter air
(NH4)2 HPO4 1.10 g
K2HPO4 5.80 g
KH2PO4 3.70 g
MgSO4 0.12 g
Mikroelemen 1 ml
Lampiran 1c. Komposisi media modifikasi garam dasar (Modified Basssal Salt
Medium, MBSM)
Uraian Konsentrasi per liter air
NH4 NO3 0.666 g
K2HPO4 6.6852 g
KH2PO4 4,1413 g
MgSO4.7H2O 0.12 g
Mikroelemen 1 ml
Lampiran 1d. Komposisi Mikroelemen
Uraian Konsentrasi per liter air
FeSO47H2O 2.80 g MnSO45H2O 2.40 g CoCl2 6H2O 2.40 g CaCl2 H2O 1.70 g CuCl2 2H2O 0.20 g ZnSO4 7H2O 0.30 g NaMoO4 0.25 g
38 Lampiran 2. Rekapitulasi hasil analisis rata-rata berat kering sel (g/l) dan
perolehan rhamnolipid (mg/l) pada produksi biosurfaktan kultivasi curah dengan variasi konsentrasi sumber karbon (minyak sawit) dan waktu fermentasi
Perlakuan
Berat kering sel (g/l)
Perolehan rhamnolipid (mg/l) Konsentrasi sumber karbon-minyak sawit (g/l) Waktu fermentasi (jam) 5 24 0,30 190 48 2,00 230 72 1,60 210 10 24 2,10 150 48 3,90 170 72 3,00 145 15 24 2,40 140 48 3,20 125 72 3,70 250
Lampiran 3. Rekapitulasi hasil analisis rata-rata berat kering sel (g/l) dan perolehan rhamnolipid (mg/l) pada produksi biosurfaktan kultivasi curah dengan variasi konsentrasi sumber karbon (limbah cair biodiesel dari CPO) dan waktu fermentasi
Perlakuan
Berat kering sel (g/l)
Perolehan rhamnolipid (mg/l) Konsentrasi sumber karbon-limbah cair biodiesel (g/l) Waktu fermentasi (jam) 5 24 0,10 70 48 0,80 50 72 0,50 47 25 24 1,70 80 48 4,20 56 72 2,80 40 50 24 1,50 72 48 2,50 48 72 3,00 80
39 Lampiran 4. Rekapitulasi hasil analisis rata-rata berat kering sel (g/l) dan
perolehan PHA (g/l) pada produksi bioplastik kultivasi curah dengan variasi konsentrasi sumber karbon (minyak sawit) dan waktu fermentasi
Perlakuan
Berat kering sel (g/l) Perolehan PHA (g/l) Konsentrasi sumber karbon-minyak sawit (g/l) Waktu fermentasi (jam) 5 24 2,60 0,05 48 1,00 0,09 72 2,00 0,07 10 24 3,00 0,40 48 1,20 0,20 72 0,60 0,30 15 24 0,03 0,10 48 0,03 0,05 72 0,01 0,04
Lampiran 5. Rekapitulasi hasil analisis rata-rata berat kering sel (g/l) dan perolehan PHA (g/l) pada produksi bioplastik kultivasi curah dengan variasi konsentrasi sumber karbon (limbah cair biodiesel dari CPO) dan waktu fermentasi
Perlakuan
Berat kering sel (g/l) Perolehan PHA (g/l) Konsentrasi sumber karbon-limbah cair biodiesel (g/l) Waktu fermentasi (jam) 5 24 0,80 0,02 48 0,30 0,01 72 0,60 0,03 25 24 1,80 0,15 48 0,60 0,10 72 0,20 0,08 50 24 0,05 0,04 48 0,03 0,02 72 0,01 0,01
40 Lampiran 6. Rekapitulasi hasil analisis rata-rata berat kering sel (g/l) dan
perolehan rhamnolipid (mg/l) pada produksi biosurfaktan kultivasi umpan curah dengan variasi konsentrasi sumber karbon (limbah cair biodiesel dari CPO) dan waktu fermentasi
Perlakuan
Berat kering sel (g/l)
Perolehan rhamnolipid (mg/l) Konsentrasi sumber karbon-limbah cair biodiesel (g/l) Waktu fermentasi (jam) 5 5 0,018 150 10 0,020 140 24 0,082 150 34 1,640 270 48 1,200 250 25 5 0,017 120 10 0,050 160 24 1,200 180 34 1,500 260 48 2,000 150 50 5 0,050 180 10 0,900 130 24 1,000 140 34 1,600 150 48 2,000 100
41 Lampiran 7. Rekapitulasi hasil analisis rata-rata berat kering sel (g/l) dan
perolehan PHA (g/l) pada produksi biosurfaktan kultivasi umpan curah dengan variasi konsentrasi sumber karbon (limbah cair biodiesel dari CPO) dan waktu fermentasi
Perlakuan
Berat kering sel (g/l) Perolehan PHA (g/l) Konsentrasi sumber karbon- limbah cair biodiesel dari CPO (g/l) Waktu fermentasi (jam) 5 5 0,15 0,01 10 0,28 0,02 24 0,44 0,04 34 0,40 0,04 48 0,52 0,05 25 5 0,20 0,10 10 0,30 0,18 24 0,85 0,23 34 0,97 0,35 48 1,20 0,30 50 5 0,01 0,01 10 0,05 0,01 24 0,12 0,06 34 0,02 0,05 48 0,02 0,05
42 Lampiran 8. Foto-foto penelitian
Biakan Pseudomonas aerugenosa dalam ampul dan proses aktivasi sel
43
Proses pemisahan sel
44
Lampiran 9. Organisasi Ketua dan Semua Anggota Tim Pengusul
No.
Nama Jabatan Dalam
Tim Tugas Dalam TIM (diuraikan dengan rinci) NIP Alokasi Waktu,
Jam/Minggu
1.
Ir. Darti Nurani, M.Si. Ketua Tim - Merencanakan penelitian - Optimasi produksi PHA
dan ramnolipid
- Konfirmasi produksi PHA dan ramnolipid
- Penulisan artikel ilmiah dan paten/HKI - Pembuatan laporan - 25 jam/minggu 2. Dr.Ir. Sidik Marsudi, M.Si.
Anggota Tim - Set up peralatan dan preparasi medium
- Analisa PHA dan ramnolipid (konsentrasi dan properti)
- Penulisan artikel ilmiah - Pembuatan laporan 19661231 199203 1 017 20 jam/minggu
45
Biodata Pengusul
1. Ketua Tim
Name : Ir. Darti Nurani,M.Si. Nationality : Indonesian
Place/Date of Birth : Magelang, July 21, 1961 Religion : Moslem
Present position : a.Head of Agroindustrial Technology Laboratory
b.Lecturer and researcher at Indonesia Institute of Technology
Home address : Telaga Kahuripan, BIP – A4/8, Parung, Bogor, Indonesia Email: n_darti@hotmail.com
Office address : Department of Agroindustrial Technology Indonesia Institute of Technology
Jl. Raya Puspiptek, Serpong, Tangerang 15320 Telp. 0217560544, Fax 7560542
Educational Background
1. 2002, Master of Science (Industrial Agricultural of Technology), Bogor Agricultural University, Bogor, Indonesia
2. 1986, Undergraduate (S1) of Agricultural Product Technology, Gajah Mada University, Yogyakarta, Indonesia
Professional Association
1. Indonesian Association of Microbiologist (PERMI), member 2. Indonesian Association of Food Technologist (PATPI), member 3. Indonesian of Society for Lactic Acid Bacteria (ISLAB), member Working Experiences
1. Lecturer in General Microbiology, Industrial Microbiology, Packaging Technology, Department of Agroindustrial Technology, Indonesia Institute of Technology, 1994 - present
2. Lecturer in Food Chemistry, Department of Agroindustrial Technology, Indonesia Institute of Technology, 1994 - present
3. Lecturer in Microbiology for Engineers, Department of Chemical Engineering, Indonesia Institute of Technology, 2004 - present
4. Secretary of Department of Agroindustrial Technology, Indonesia Institute of Technology, 1991 - 1992
5. Head of Microbiology Laboratory, Department of Agroindustrial Technology, Indonesia Institute of Technology, 1992 – 1997; 2002 - 2008 6. Head of Agroindustrial Technology Laboratory, Indonesia Institute of
Technology, 2008 – present
7. Organizing Committee in Symposium Biotechnology of Probiotic for Human Health, Center for Assessment of Biotechnology collaboration with Shinshu University, Japan and National University of Singapore, Jakarta 2002
8. Organizing Committee in International Symposium on Probiotic, Center for Assessment of Biotechnology collaboration with Japan Collection of
46 Microorganism – RIKEN and National University of Singapore, Bali 2004 9. Organizing Committee in PATPI Congress and Seminar, Jakarta 2004
10. Organizing Committee in The 4th Indonesian Biotechnology Conference: Biotechnology for Better Food, Health and Environment, Bogor 5 – 7 August 2008
11. Organizing Committee in International Symposium on Probiotic from Asian Traditional Fermented Foods for Healthy Gut Function, SEAMEO – TROPMED Regional Center for Community Nutrition University of Indonesia, Jakarta August 19th – 20th 2008
Course
1. Marine Pollution Monitoring and Training Programme, LIPI – UNESCO/UNDP, Puslitbang Oceanologi, LIPI Jakarta, November 20 – 23, 1989
2. Seminar Ilmiah Persatuan Ahli Teknologi Laboratorium Kesehatan Indonesia (Patelki): Peranan pengawasan mutu untuk peningkatan dan pengendalian keamanan produk pangan dalam menyongsong UU Pangan dan era pasar bebas, Jakarta November 25 – 26, 1996
3. International Seminar and Exhibition: Release and Biosafety of Genetically Modified Foods, Bogor Agricultural University, September 10 – 11, 1997 4. One day seminar on The Challenging Prospect and Strategies of Yeast
Diversity and National Workshop on Isolation and Identification of Yeast, August, 19 – 23 1999, Wisma Makara, University of Indonesia
5. Indonesian International Joint Research Grant Program (RUTI): Workshop The First Year Result: In vivo and clinical studies and probiotic properties of indigenous Dadih lactic bacteria for starter culture. December, 17 2002. Balai Pengkajian Bioteknologi, BPPT, Serpong
6. Kursus identifikasi mikroba, August 27 – 28 2003. PAU, ITB, Bandung 7. Visiting Researcher University of Turku, Finland. October 2006 – February
2007
Research activities
1. Screening of Lactic Cultures Isolated from Dadih Bukittinggi for Fermented Culture, 1996 - 1997
2. Amino Acid Profiles of Some Lactic Acid Bacteria from Dadih, 1997 – 1998 3. The Effect of Preserving Lactic Acid Bacteria Isolated from Dadih on Viability, Biochemical Activity and Antimutagenicity of The Bacterial Cells. URGE Project, Batch III, 1998/2000
4. Exploration of Indigenous Lactic Acid Bacteria from Dadih from West Sumatra for Good Starter Culture and Probiotic Bacteria (URGE – DCRG 2000 - 2001)
5. In Vivo and Clinical Studies on Probiotic Properties of Indigenous Dadih Lactic Acid Bacteria for Starter Culture, RUTI, Assessment of Biotechnology, BPPT 2001 – 2003
6. Study on Starter Culture Media Formulation of Yoghurt Probiotic. 2003 7. Viability of Probiotic in Cream Biscuit Storage. 2003
8. Optimatization Condition for α – amylase Extracelluler Production. 2003 9. Characterization Probiotic Properties of Lactic Acid Bacteria Isolated from
47 “Badeg Pace”. 2004
10. Pengaruh Konsumsi Probiotik pada Aktivitas Mikrobiologis Saluran Cerna. 2006
11. Probiotic for Decontaminating Microcystin Containing Indonesia Water Samples. University of Turku, Finland, October 2006 – February 2007 12. Assessment of Probiotic Lactobacillus plantarum from Dadih for Human
Health Promotion. Applied Research Program SEAMEO – TROPMED, University of Indonesia. 2007
13. Production of Probiotic Concentrate IS-725 for The Powder Milk Product. 2008
14. 15.
Investigation any Bacteriocin Produced by Lactic Acid Bacteria Isolated from Dadih. 2008
Pengaruh suplementasi yoghurt probiotik asal Dadih Enterococcus faecium, IS-27526 terhadap morbidas diare dan respon imun IgA secretory pada balita. 2009. Penelitian Isu Strategis (UNJ), dana DIKTI
Publication
1. Nurani, D. dan I.S. Surono, 1996. Pengaruh cara pengeringan dan lama penyimpanan pada karakteristik jamur pangan kering. Jurnal IPTEK –
Institut Teknologi Indonesia, No V, November 1996
2. Nurani, D., T. Tedja, A. Suryani, I.S. Surono, 2000. Viabilitas dan aktivitas biokimiawi pada pembekuan dan penyimpanan beku kultur bakteri laktat asal Dadih. Jurnal IPTEK – Institut Teknologi Indonesia,
No XVIII, September 2000
3. Surono, I.S., D. Nurani dan A.A.A. Dharmawati, 1997. Seleksi kultur
bakteri laktat asal Dadih Bukittinggi sebagai starter susu fermentasi.
Jurnal IPTEK – Institut Teknologi Indonesia, No VII, Agustus 1997 4. Nurani, D., E. Martius, I.S. Surono dan Koesnandar, 2003. Study on
Starter Culture Media Formulation of Yoghurt Probiotic. Buku Program
dan Abstrak PIT – PERMI, 29 – 30 Agustus 2003, Bandung
5. Trismilah, B. Wahyuntari dan D. Nurani, 2004. Pemanfaatan berbagai
jenis pati sebagai sumber karbon untuk produksi α-amilase ekstraseluler Bacillus SW2. Buku Program dan Abstrak PIT – PERMI, Agustus 2004,
Semarang
6. Surono, I.S., U. Pato, Koesnandar, D. Nurani and A. Latief, 2004. Dadih
Probiotic: In Vivo and Clinical Studies on Lactic Bacteria IS-27526.
Programme/Abstract of International Symposium Probiotic for Human Health and Immunity. Bali 7 – 8 September 2004
7. Surono, I.S., F. Riewpassa, D. Nurani, C.M. Kusharto, 2004. In vivo
Immune Response of Spraque Dawley Rat Fed with Casein, Fish Protein Concentrate Biscuit. Programme/Abstract of International Symposium
Probiotic for Human Health and Immunity. Bali 7 – 8 September 2004 8. Surono, I.S., Koesnandar, F.R. Zakaria, D. Nurani, F. Koestomo, N.
Novitasari, J. Dharmawan, Yuan Kun Lee, 2005. Immunodulatory
Properties of Dadih Probiotic Bacteria in Indonesian Undernourished Children. Buku Program dan Abstrak PIT – PERMI, 25 – 28 Agustus
48 9. A. Amar, D. Nurani, F.A. Wijaya, 2008. Isolation and Identification of
Bacteria in Kombucha from Serpong, Tangerang, Banten. Buku Program
Abstrak The 4th Indonesian Biotechnology Conference: Biotechnology for Better Food, Health and Environment, Bogor 5 – 7 August 2008
10.
11.
12.
Nurani, D., M.H. Thayeb, K.A. Prasetya, 2008. Daya Simpan “Indonesian Dressing” Berbahan Baku Kacang Mete. Buku Program dan
Abstrak Seminar dan Kongres PATPI, 14 – 16 Oktober 2008, Palembang 11 Nurani, D., S. Sukotjo dan B.U.D. Putra, 2009. Karakteristik Probiotik
secara in vitro Bakteri Asam Laktat asal Badeg Pace. Prosiding Seminar
Nasional PATPI, 3 – 4 november 2009, Jakarta.
Surono, I.S., A. Khomsan, E. Sobariah and D. Nurani, 2009. Effect of
Oxygenated Water and Probiotic Administration on Fecal Microbiota of Rats. Programme/Abstract of International Conference of Indonesian
society for Microbiology, 20 – 21 November 2009, Surabaya
Serpong, Nopember 2011
49 2. Anggota Tim
Name : Dr. Ir. Sidik Marsudi,M.Sc.
Gender : Male
Place/Date of birth : Cot Girek/March 17, 1966
Nationality : Indonesia
Current position : Lecturer at Indonesia Institute of Technology,
Jalan Raya Puspiptek, Serpong, Tangerang, Indonesia.
Address : Jalan Sukabakti VI No.36, Tangerang 15118, Indonesia.
Phone : +62-21-5524-163 (home), +62-813-1125-1231 (mobile)
Email address : sidikmar@hotmail.com
Status : Government employee
Id Number : 131 993 522/19661231 199203 1 017
Work experience
2004 – now Lecturer at Chemical Engineering Department, Industrial
Engineering Faculty, Indonesia Institute of Technology, Serpong, Tangerang, Indonesia
1990 - 2004 Lecturer at Chemical Engineering Department, Engineering
Faculty,
Syiah Kuala University, Banda Aceh, Indonesia
Nov, 2002 – Oct, 2003 Postdoctorate researcher at Faculty of Science, Malaya
University; Kuala Lumpur.
Responsible for serving The Summirubber Consultancy project (Summirubber company / Summitomo group – Malaya University collaboration project) in polyhydroxyalkanoates (bioplastic) production by employing bacteria using a fermenter.
Administrative experience
2005 – 2010
1997-1998
Head of Chemical Engineering Department, Engineering
Faculty, Indonesia Institute of Technology,
Serpong,Tangerang, Indonesia
Head of Biotechnology Laboratory, Department of Chemical Engineering, Syiah Kuala University, Indonesia
Education
2002 Doctor of Engineering in Biotechnology, Tokyo Institute of
Technology (TIT), Tokyo, Japan.
Dissertation
“Simultaneous Microbial Production of polyhydroxyalknaotes (PHAs) and Rhamnolipids”
Summary:
To overcome PHAs (bioplastic) production cost, a
simultaneous production of PHAs and rhamnolipid
50
Pseudomonas aeruginosa. The result showed that it was
feasible to produce both bio-products simultaneously and destroyed a common belief that production cost of PHA will never lower than to produce the cell it’s self.
1997 Master of Science in Chemical Engineering, Bandung
Institute of Technology (ITB), Bandung, Indonesia
Master thesis
“Preliminary study of polyhydroxyalknaotes (PHAs)
biosynthesis by using Rhodobacter sphaeroides photosynthetic bacterium on volatile fatty acids as carbon sources”
Summary:
Biosynthesis of PHAs was studied at various C/N ratios. Experiments were conducted in two steps, i.e. cell production, and PHA production step. Medium without nitrogen source gave the highest production rate in PHA accumulation step.
1990 Bachelor of Engineering in Chemical Engineering , Faculty
of Engineering, Syiah Kuala University, Banda Aceh, Indonesia
Under graduate research:
“Effect of plate shape for water-air contact on atmospheric cooling water”
Summary:
Several types of wood-plate were designed to give a better contact between air and hot water to reduce water temperature. Rectangular shape gave the highest reducing temperature rate from an initial water temperature of 90 C to 30 C.
Under graduate thesis
“Design of Benzene Plant from Toluene and Hydrogen”
Summary:
Benzene plant/factory was designed to produce benzene using toluene and hydrogen as raw materials. The major design was benzene reactor at a capacity of 60.000 ton/year. The design included the calculation of pumps required, distillation column, utilities, and other facilities required for benzene factory
Training 2007
(August -October)
“Health Safety and Environment (HSE)” training course at University of Occupational Envionment Health (UOEH), Fukuoka, Japan
A training course held by JICA in respect to the HSE application in industries for develeping counties
51
2006
(June – August)
“Waste water treatment”, internship at Nagoya Institute of Technology (NIT), Nagoya, Japan
An internship on how to treat food waste containing highly oily waste.This internship was supported by TPSDP Project
1997
(Nov 1997– March 1998)
“Engineering Education”, at Biotechnology Department, Tokyo Institute of Technology (TIT), Tokyo, Japan.
Studied how to teach students for doing research especially parameters that required to be determined in the field of biochemical engineering.
1991
(August-October)
“Introduction to Microbiology”, at Inter University Center (IUC) for Biotechnology, Gajah Mada University (UGM), Yogyakarta, Indonesia
Studied how to work with microorganism, how to operate a fermenter: Batch culture, feed batch culture, and continuous culture.
1989
(July – August)
“Job training”, P.T. Cement Andalas Indonesia, Banda Aceh, Indonesia
Studied the processes that were involved in “dry process” of cement production plant, which used limestone and gypsum as raw materials.
Research grand award
1. Simultaneous Production of polyhydroxyalkanoates
(PHAs) and Rhamnolipid by Pseudomonas sp using CPO as a carbon source
Research team:
1. Dr. Ir. Sidik Marsudi, M.Sc.(principal Investigator) 2. Dr. Tri Panji M.S. APU (member)
3. Dr. Ir. Ratna Siri (Member)
Funding source:
Integrated prime research (RUT XII), Minister of Research and Technology, Indonesia, 2005 – 2006.
2. Production of bioactive compost and biogas from solid and liquid waste of palm oil mill
Research team:
1. Dr. Ir. Siswanto, APU.(principal Investigator) 2. Dr. Ir. Sidik Marsudi, M.Sc.(member) 3. Ir. Suharyanto, M.Si (member)
4. Isroi, S.Si.,M.Si.
Funding source:
Collaboration Prime Research (RUK XI), Minister of Research and Technology, Indonesia, 2005 – 2006.
52
3. Recovery of Polyhydroxyalkanoates (PHAs) by enzymatic Process
Research team:
1. Dr. Ir. Sidik Marsudi, M.Sc. (principal investigator)
2. Dr. Ir. Dwi Susilaningsih (member)
Funding source:
Indonesia Toray Science Foundation (ITSF), Jakarta, Indonesia, 2004
International Publication
1. Marsudi, S., Unno, H., and Hori, K., 2008, Utilization of palm oil on simultaneous production of polyhydroxyalkanoates (PHAs) and rhamnolipid by
Pseudomonas aeruginosa using palm oil as a carbon source. Applied
Microbiology and Biotechnology 78: 955-961
2. Hori, K., Marsudi, S., and Unno, H., 2002, Simultaneous production of polyhydroxyalkanoates (PHAs) and rhamnolipid by Pseudomonas
aeruginosa, Biotechnology and Bioengineering,78(6),699-707.
This work demonstrated the possibility to produce PHAs and rhamnolipid simultaneously using decanoic acid or glucose as a carbon source.
Conference/Seminar/Publication
Marsudi, S., Tan, I.K.P., Gan, S.N., and Ramachandran, K.B., 2007, “Production of
medium-chain-length Polyhydroxyalkanoates (PHA-mcl) from oleic acid using Pseudomonas Putida PGA1 by fed batch culture, 11 (1), 1-4, Makara seri Teknologi, Scientific Journal of Indonesia University, Depok, Jakarta
Marsudi, S, 2006, Recovery of Polyhydroxyalkanoates (PHAs) by enzymatic Processes,
Reaktor, 10 (2), 59-62, Sientific Journal Chemical Engineering Department, Diponegoro University, Semarang
Marsudi, S., Tan, I.K.P., Gan, S.N., and Ramachandran, K.B., 2003, “Production of
medium-chain-length Polyhydroxyalkanoates (PHA-mcl) in fermenter using saponified crude palm oil (SCPO) and saponified crude palm kernel oil (SCPKO) as carbon substrates. International Conference on Chemical and Bioprocess Engineering (iccbpe 2003), University of Malaysia Sabah