BAB V KESIMPULAN DAN SARAN
B. Saran
1. Perlu dilakukan proses pengujian lebih lanjut untuk mengetahui senyawa-senyawa yang terkandung di dalam ekstrak etanol daun L. officinalis Chaix. 2. Potensi ketoksikan dari ekstrak etanol daun L. officinalis Chaix perlu
ditingkatkan dengan mengujikannya pada model sel kanker payudara lain. 3. Penelitian dilanjutkan dengan membuat suatu formulasi sediaan
menggunakan ekstrak etanol daun Lavandula officinalis Chaix sebagai obat kemopreventif.
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Lampiran 1. Surat keterangan hasil determinasi sampel daun L. officinalis Chaix
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Lampiran 2. Perhitungan seri kadar ekstrak etanol daun L. officinalis Chaix dan tamoxifen
a. Ekstrak etanol daun L. officinalis Chaix 10 mg + 100 µL DMSO = 100.000 µg/mL
Intermediet (int): 2000 µg/mL= 30 µL bulk + 1470 µL MK C1 (1585 µg/mL) = 396 µL (int) + 104 µL MK C2 (1150 µg/mL) = 288 µL (int) + 212 µL MK C3 (1000 µg/mL) = 250 µL (int) + 250 µL MK C4 (300 µg/mL) = 75 µL (int) + 425 µL MK C5 (178 µg/mL) = 45 µL (int) + 455 µL MK C6 (100 µg/mL) = 50 µL C3 + 450 µL MK C7 (10 µg/mL) = 50 µL C7 + 450 µL MK b. Tamoxifen (2000 µM) C1 (315 µg/mL) = 79 µL bulk + 421 µL MK C2 (175 µg/mL) = 44 µL bulk + 456 µL MK C3 (100 µg/mL) = 25 µL bulk + 475 µL MK C4 (60 µg/mL) = 15 µL bulk + 485 µL MK C5 (35 µg/mL) = 9 µL bulk + 491 µL MK C6 (20 µg/mL) = 5 µL bulk + 445 µL MK C7 (10 µg/mL) = 50 µL C7 + 450 µL MK C8 (1 µg/mL) = 50 µL C7 + 450 µL MK
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Lampiran 3. Tabel viabilitas sel T47D dengan pemberian ekstrak etanol daun L. officinalis Chaix.
1 1585 3,200 2 1150 3,061 3 1000 3 5 300 2,477 6 178 2,250 7 100 2 8 10 1 LOG+KONSENTRASI KONSENTRASI NO A1 A2 A3 rata(rata 0,105 0,12 0,103 0,109 0,111 0,115 0,108 0,111 0,095 0,093 0,094 0,094 0,12 0,139 0,173 0,144 0,68 0,736 0,752 0,723 0,756 0,799 0,792 0,782 0,644 0,775 0,771 0,730 ABSORBANSI V1 V2 V3 RATA(RATA) SD 1,428 3,973 1,089 2,163 1,576 2,446 3,125 1,937 2,502 0,596 ,0,269 ,0,608 ,0,438 ,0,438 0,170 3,973 7,196 12,965 8,045 4,556 98,982 108,483 111,198 106,221 6,414 111,876 119,171 117,984 116,344 3,914 92,874 115,100 114,421 107,465 12,640 %)VIABILITAS)SEL
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Lampiran 4. Tabel viabilitas sel T47D dengan pemberian tamoxifen.
1 1000 3 2 100 2 3 10 1 4 1 0 5 0,1 &1 6 0,01 &2 LOG*KONSENTRASI KONSENTRASI NO A1 A2 A3 rata(rata 0,122 0,128 0,125 0,125 0,107 0,11 0,111 0,109 0,789 0,784 0,759 0,777 0,778 0,744 0,692 0,738 0,761 0,776 0,769 0,769 0,669 0,742 0,701 0,704 ABSORBANSI V1 V2 V3 RATA(RATA) SD 4,233 5,163 4,698 4,698 0,465 1,910 2,375 2,530 2,272 0,322 107,537 106,763 102,891 105,731 2,489 105,834 100,568 92,514 99,639 6,708 103,201 105,524 104,440 104,388 1,162 88,952 100,258 93,908 94,373 5,667 %)VIABILITAS)SEL
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Lampiran 5. Hasil analisis IC50 ekstrak etanol daun L. officinalis Chaix menggunakan program R.
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Lampiran 7. Pengamatan tiga lapang pandang sel T47D dalam perhitungan imunositokimia
A. Perlakuan esktrak etanol daun L. officinalis Chaix.
B. Perlakuan tamoxifen
C. Kontrol sel dengan pemberian antibodi
D. Kontrol sel tanpa antibodi
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BIOGRAFI PENULIS
Winda Sekarjati. Penulis lahir pada tanggal 28 Agustus 1992 di Yogyakarta dan merupakan anak kedua dari tiga bersaudara, memiliki kakak bernama Datia Kurniawitri dan adik bernama Pandu Yogaswara. Penulis lahir dari pasangan suami- istri Sudarto dan Tatik Retno Wulan dan telah menempuh pendidikan di SD Lempuyang Wangi pada tahun 1999 sampai tahun 2005, SMP Negeri 5 Yogyakarta pada tahun 2005 sampai dengan tahun 2008, SMA Negeri 8 Yogyakarta pada tahun 2008 sampai dengan tahun 2011, dan melanjutkan pendidikan di Fakultas Farmasi, Universitas Sanata Dharma pada tahun 2011 sampai dengan tahun 2015. Selama menjadi mahasiswa, penulis mengikuti beberapa kegiatan dan kepanitiaan, yaitu Program Kreativitas Mahasiswa bidang Pengabdian Masyarakat lolos didanai DIKTI (2013), Inisiasi Fakultas Farmasi yaitu TITRASI (Tiga Hari Temu Akrab Farmasi ) selama tiga tahun berturut- turut (2012-2014) menjabat sebagai anggota seksi acara, koordinator seksi acara dan
steering committee (SC), Pelatihan Pengembangan Kepribadian Mahasiswa
(PPKM I) sebgai Co- Facilitator (2013), serta pernah mengambil pekerjaan part
