BAB V. KESIMPULAN DAN SARAN
B. Saran
Peneliti menyarankan bahwa perlu dilakukan penelitian lebih lanjut mengenai:
1. Kemampuan ekstrak etanol kulit P. americana untuk menurunkan konsentrasi ALP harus diujikan pada hewan uji yang terpapar agen toksik lain seperti parasetamol.
2. Adanya penambahan hewan uji untuk memperkecil penyimpangan standar deviasi.
3. Adanya gambaran histopatologi hepar hewan uji terinduksi karbon tetraklorida untuk mengetahui tingkat kerusakan hepar.
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Lampiran 1. Foto buah dan serbuk kulit P. americana
Lampiran 2. Foto proses ekstraksi
Lampiran 3. Foto ekstrak etanol kulit P. americana dan ekstrak etanol + CMC-Na
Lampiran 5. Surat pengesahan Medical and Health Research Ethics Commitee (MHREC)
Lampiran 7. Hasil orientasi
Lampiran 8. Hasil perlakuan 1. Uji Normalitas
Multiple comparison Scheffe
Kelompok Kontrol Hepatotoksin CCl4 2 ml/kg BB Kontrol negatif olive oil 2 ml/kg BB Kontrol EtOH Panjang 1400 mg/kg BB Dosis 1 EtOH panjang 350 mg/kg BB Dosis 2 EtOH panjang 700 mg/kg BB Dosis 3 EtOH panjang 1400 mg/kg BB Kontrol Hepatotoksin CCl4 2 ml/kg BB BB BB TB TB BB Kontrol negatif olive oil 2 ml/kg BB BB TB TB TB TB Kontrol EtOH Panjang 1400 mg/kg BB BB TB BB BB TB Dosis 1 EtOH panjang 350 mg/kg BB TB TB BB TB TB Dosis 2 EtOH panjang 700 mg/kg BBTB TB TB BB TB TB Dosis 3 EtOH panjang 1400 mg/kg BB BB TB TB TB TB
Kelompok Perlakuan Rata-rata konsentrasi ALP ± SE (U/L) Efek Hepatoprotektif ALP (%) I Kontrol CCl4 440,2±37,727 -
II Kontrol Olive Oil 274,2±25,6 0
III Kontrol sediaan
(ekstrak etanol kulit Persea americana dosis 1400 mg/kg BB 190,2±12,08 - IV Dosis I esktrak etanol kulit Persea americana Mill. (350 mg/kg BB) 395,6±34,50 13,989 V Dosis II esktrak etanol kulit Persea americana Mill. (700 mg/kg BB) 336,6±10,48 32,496
VI Dosis III esktrak
etanol kulit Persea americana Mill. (1400 mg/kg BB)
Lampiran 9. Perhitungan Rendemen Ekstrak Etanol Kulit P. americana Hasil rendemen ekstrak etanol kulit P. americana
Ket (gram) C-1 C- 2 C- 3 C- 4 C- 5 C- 6 C-7,8 C- 9.10 Cawan kosong 58,8 48,9 36,6 52,6 59,0 56,7 65,0 65,7 Cawan+ ekstrak 64,1 53,6 42,9 58,3 64,2 61,3 68,7 75,0 Rendem en 5,3 4,7 6,3 5,7 5,2 4,6 3,7 9,3 Keterangan : C=cawan Rata-rata rendemen = = , , , , , , , , = 4,48 gram
% rendemen ektrak kental = , % = 11,20 %
Hasil menunjukkan bahwa sebanyak 400 gram serbuk kering kulit P. americana menghasilkan 10 cawan ekstrak kental. Rata-rata rendemen setiap cawan 4,48 gram ekstrak kental. Pada pembuatan 400 gram serbuk kering kulit P. americana menghasilkan 44,8 gram ekstrak kental, dengan rendemen 11,20 %.
Bobot pengeringan ekstrak etanol-air biji P. americana Berat cawan kosong (gram) Jam ke 0 2 4 6 8 10 12 48,9 Berat ekstrak (gram) 58,5 54,9 54,1 53,8 53,7 53,6 53,6 36,6 51,0 45,1 43,7 43,2 43,0 42,9 42,9 59,0 71,5 66,1 64,9 64,6 64,4 64,2 64,2
BIOGRAFI PENULIS
Penulis skripsi dengan judul ”Pengaruh Pemberian Jangka Panjang Ekstrak Etanol Kulit Persea americana Mill. terhadap Aktivitas Alkalin Fosfatase pada Tikus Jantan Galur Wistar Terinduksi Karbon Tetra Klorida” memiliki nama lengkap Lusia Drikti Nini Gorantokan. Penulis lahir di Kalabahi pada tanggal 15 Maret 1993, merupakan anak pertama dari pasangan Agustinus Lebu Raya dan Elisabeth Sulastri Soedjono. Penulis mengawali masa pendidikannya di TK St. Theresia Lipa Kalabahi (1996-1999) kemudian melanjutkan ke bangku SDK St. Maria Lipa Kalabahi (1999-2005). Penulis menyelesaikan pendidikan tingkat sekolah menengah pertama di SMPN 1 Kalabahi (2005-2008), kemudian melanjutkan pendidikan di SMA Marsudirini Muntilan (2008-2011). Penulis kemudian melanjutkan pendidikan di sarjana di Fakultas Farmasi Universitas Sanata Dharma Yogyakarta pada tahun 2011. Penulis aktif dalam berbagai kepanitiaan seperti anggota divisi P3K Pharmacy Performance (2011), Pharmacy Performance and Even Cup (2012), Sie konsumsi dan usaha dana kepanitian Desa Mitra (2012), anggota UKF Basket (2011-2013). Penulis juga aktif di organisasi di luar kampus yaitu Pendamping Iman Anak (PIA) Kotabaru dan menjabat sebagai sekretaris (2013-2014), dan saat ini masih aktif sebagai anggota PIA Kotabaru. Penulis pernah menjadi asisten praktikum Kimia Dasar (2013-2014), dan Komunikasi Farmasi (2014).
