V. KESIMPULAN DAN SARAN
5.2. Saran
Dari hasil penelitian marka molekuler ini akan dikembangkan dengan beberapa penelitian selanjutnya yaitu:
1. Aplikasi sistem marka molekuler dapat diujikan pada berbagai jenis ikan lainnya.
2. Marka molekuler, GH dan vasa akan diaplikasikan untuk menguji kolonisasi sel donor ikan gurame.
DAFTAR PUSTAKA
Alimuddin. 2005. Teknik baru menyelamatkan ikan langka.
Alimuddin, Junior MZ, dan Arfah H. 2009. Teknologi transplantasi sel testikular dalam rekayasa produksi benih ikan gurame (Osphronemus gouramy). Direktorat Jenderal Pendidikan Tinggi. 32 p.
Brown TA. 1995. Gene cloning and introduction. London: Chapman & Hall. Dale JW, and Schantz MV. 2002. From genes to genomes: concepts and
applications of DNA technology. John Wiley & Sons Ltd, England.
Dalgleish R. 2007. The Polymerase Chain Reaction (PCR).
pdf [Diakses tanggal17 April 2009].
DKP. 2005. Revitalisasi: perikanan budidaya 2006-2009. 275 p. Erlich HA. 1989. PCR Technology. M Stocton Press. 246 p.
Fujimura K, and Okada N. 2007. Development of embryo, larva and early juvenile of Nile tilapia Oreochromis niloticus (pisces: chiclidae) developmental staging system. Develop Growth Differ. 49: 301-324
Griffith AJF, Wessler SR, Lewontin RC, Gelbart WM, Suzuki DT, and Miller JH. 2005. An Introduction to Genetic Analysis. W.H. Freeman and Company. America
Hill JR, and Dobrinski I. 2006. Male germ cell transplantation in livestock.
Reproduction, Fertility and Development. 18: 13-18
Karanis P, Thekisoe O, Kiouptsi K, Ongerth J, Igarashi I, and Inoue N. 2007. Development and preliminary evaluation of a loop-mediated isothermal amplification procedure for sensitive detection of Cryptosporidium
oocysts in fecal and water samples. Applied and Environmental Microbiology. 73 (17): 5660–5662
Kobayashi S, Alimuddin, Morita T, Miwa M, Lu J, Endo M, Takeuchi T, and Yoshizaki G. 2007. Transgenic Nile tilapia Oreochromis niloticus over-expressing growth hormone show reduced ammonia excretion.
Aquaculture. 270: 427-435.
Lacerda SMSN, Batlouni SR, Silva SBG, Homem CSP, and Franca LR. 2006. Germ cells transplantation in fish: the Nile-tilapia model. Anim. Reprod. 3: 146-159
Liu ZJ, Li P, Argue BJ, and Dunham RA. 1999 Random amplified polymorphic DNA markers: usefulness for gene mapping and analysis of genetic variation of catfish. Aquaculture.174:59–68.
Melamed P, Rosenfelt, Elizur A, and Yaron Z. 1998. Endocrin regulation of gonadotrophin and growth hormone gene transcription in fish. Comp Biochem Physiol C Pharmacol Toxicol Endocrinol. 119:325-338.
Newton CR & Graham A. 1994. PCR. Oxford, U.K.: BIOS Scientific Publishers.
diacu dala
[Diakses tanggal 21 Juli 2009]
Nichole DST. 2002. An introduction to genetic engineering second edition. Cambridge University Press. 287 p.
Nóbrega RH, Batlouni ASR, and França ALR. 2009. An overview of functional and stereological evaluation of spermatogenesis and germ cell transplantation in fish. Fish Physiol Biochem. 35:197-206.
Nugroho E, Alimuddin, Kristanto AH, Carman O, Megawati N, Sumantadinata K. 2008. Kloning cDNA hormon pertumbuhan dari ikan gurame (Osphronemus gouramy). J. Ris. Akuakultur. 3:183-190.
Okutsu T, Yano A, Nagasawa K, Shikina S, Kobayashi T, Takeuchi K, and Yoshizaki G. 2006a. Manipulation of fish germ cell: visualization, cryopservation and tranplantation. J.Reprod. Dev. 52:685
Okutsu T, Suzuki K, Takeuchi, Y, Tekeuchi T, and Yoshizaki G. 2006b. Testicular germ cells can colonize sexually undifferentiated embryonic gonad and produce functional egg in fish. Proc. Natl. Acad. Sci. U.S.A. 103:2725-2729
Okutsu T, Takeuchi Y, and Yoshizaki G. 2008. Spermatogonial transplantation in fish: Production of trout offspring from salmon parents. In: Fisheries for global Welfare and environment, 5th World Fisheries Congress. Tsukamoto K, Kawamura T, Takeuchi T, Beard TD, Kaiser MD.(Ed.), Terrapub, p 209-219
Olsen LC, Aasland R, and Fjose A. 1997. A vasa-like gene in zebrafish identifies putative primordial germ cells. Mech. Dev. 66:95-105
Onodera K. 2007. Selection for 3-End Triplets for Polymerase Chain Reaction Primers. in Yuryev A. (editor). 2007. Methods in Molecular Biology: PCR Primer Design. Humana Press, Totowa, NJ. 402: 415 p.
Rasmussen R. 1992. Optimizing Rapid Cycle DNA Amplification Reactions.
The RapidCylist Newsletter.1(1):77-83AT
SETTING UP SAMPLE
Sawatari E, Shikina S, Takeuchi T, and Yoshizaki G. 2007. A novel ransforming growth factor-β super family member expressed in gonadal somatic cells
enhances primordial germ cell and spermatogonial proliferation in rainbow trout (Oncorhynchus mykiss). Developmental Biology. 301:266-275
Stickney RR. 2000. Tilapia culture. In Stickney RR (Ed.). Encyclopedia of aquaculture. New York, USA: John Wiley & Sons. pp.934-941.
Takeuchi Y, Yoshizaki G, Kobayashi T, and Takeuchi T. 2002. Mass isolation of primordial germ cells from transgenic rainbow trout carrying the green fluorescent protein gene driven by the vasa gene promoter. Biologi of Reproduction. 67: 1087-1092
Takeuchi Y, Yoshizaki G, and Takeuchi T. 2003. Generation of live fry from intraperitonally transplanted primordial germ cells in rainbow trout.
Biologi of Reproduction. 6: 1142-1149.
Takeuchi Y, Yoshizaki G, and Takeuchi T. 2004. Surrogate broodstock produces salmonids. Nature. 430:629-630.
Trewavas E. 1983. Tilapiine fishes of the genera Sarotherodon, Oreochromis
and Danakilia. British Mus. Nat. Hist. 583 p
Volckaert FA, Hellemans BA, Galbusera P, and Ollevier F. 1994. Replication, expression and fate of foreign DNA during embryonic and larval development of the African catfish Clarias gariepinus. Molecular Marine Biology and Biotechnology 3: 57-69
Wright PA, and Land MD. 1998. Urea production and transport in teleost fishes.
Comp Biochem Physiol. Physiol A: Mol Integr Physiol. 119:47-54
Walker JM and Rapley R. 2002. Molecular Biology and Bio technology Fourth Edition. The Royal Society of Chemistry. 555 p.
Wallace RB, Shaffer J, Murphy RF, Bonner J, Hirose T, and Itakura K. 1979. Hybridization of synthetic oligodeoxynucleotides to fX174 DNA: the effect of single base pair mismatch. Nucleic Acids Res. 6: 3543–3557. Yano A, Suzuki K, Yoshizaki G. 2008. Flow-cytometric isolation of testicular
germ cells from rainbow trout (Oncorhynchus mykiss) carrying the green fluorescent protein gene driven by trout vasa regulatory regions. Biol Reproduction. 78:151-158.
Yoon C, Kawakami K, and Hopkins N. 1997. Zebrafish vasa homologue RNA is localized to the cleavage planes of 2- and 4-cell-stages embryos and is expressed in the primordial germ cells. Development. 124:3157-3166
Yoshizaki G, Takeuchi Y, Sakatani S, and Takeuchi T. 2000. Germ cell-specific expression of green fluorescent protein in transgenic rainbow trout under control of the rainbow trout vasa-like gene promoter. Int. J. Dev. Biol. 44:323-326.
Yoshizaki G, Takeuchi Y, Kobayashi T, Ihara S, Takuchi T. 2002. Primordial germ cells: the blueprint for a piscine life. Fish Physiology and Biochemistry. 26: 3-12.
Yoshizaki G, Tago Y, Takeuchi Y, Sawatari E, Kobayashi T, and Takeuchi T. 2005. Green fluorescent protein labeling of primordial germ cells using a nontrangenik method and its application for germ cell transplantation in salmonidae. Biology of Reproduction. 73: 88-93
Zapata RF. 2009. Expression of GFP in transgenic tilapia under the control of the medaka β-Actin promoter: establishment of a model system for germ cell transplantation. [THESIS]. Tokyo University of Marine Science and Technology. 45 p.
Lampiran 2. Penentuan suhu annealing berdasarkan jumlah basa nukleotida dan persentase GC berdasarkan tabel Tm (oC)