SARAN

BAB 6 PEMBAHASAN, SIMPULAN DAN SARAN

6.3. SARAN

• Perlu dilakukan suatu penelitian serupa dengan jumlah sampel yang jauh lebih besar agar didapat hasil yang lebih bermakna mengenai gambaran S100 pada schwanoma, juga agar diidapat hubungan antara suku dengan insidensi schwanoma.

• Penelitian lanjutan disarankan dilakukan dengan melibatkan banyak pusat kesehatan yang memiliki faslitas pemeriksaan histopatologi yang akurat serta pemeriksaan imunohistokimia yang tepat pula.

DAFTAR PUSTAKA

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Cetinkal A, Atabey EC, Kaya ES, et al.2009. Intraosseous Schwannoma of thoracic 12 vertebra without spinal canal involvement. Eur Spine J;18(Suppl 2):S236-S239

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SUSUNAN PENELITI

Peneliti

a. Nama Lengkap : dr. Muhammad Chairul

b. Pangkat : -

c. Jabatan Fungsional : -

d. Fakultas : Kedokteran

e. Perguruan Tinggi : Universitas Sumatera Utara

Pembimbing I

a. Nama Lengkap : DR. dr. Ridha Darmajaya, Sp. BS

b. NIP. : 19730514 200212 1 002

c. Jabatan Fungsional : Staff Departemen Ilmu Bedah Saraf Fakultas Kedokteran Universitas Sumatera Utara.

d. Fakultas : Kedokteran

e. Perguruan Tinggi : Universitas Sumatera Utara f. Bidang Keahlian : Ilmu Bedah Saraf

Pembimbing II

a. Nama Lengkap : Prof. dr. Abdul Gofar S., SpBS(K).

b. Pangkat / Gol. / NIP. : Guru Besar / IVd / 19440507 197703 1 001

c. Jabatan Fungsional : Kepala Program Studi Departemen Ilmu Bedah Saraf Fakultas Kedokteran Universitas Sumatera Utara.

d. Fakultas : Kedokteran

e. Perguruan Tinggi : Universitas Sumatera Utara f. Bidang Keahlian : Ilmu Bedah Saraf

RENCANA ANGGARAN PENELITIAN

No. Uraian Jumlah

1.

Biaya Pemeriksaan IHC Protein S100 di Murni Teguh Memorial Hospital

@ Rp 150.000 X 17 Sampel = Rp 2.550.000

2. Penggandaan dokumen Rp 500.000

3. Pembuatan Proposal dan Laporan Penelitian Rp 250.000

4. Penggandaan proposal dan Laporan penelitian Rp 500.000

JADWAL PENELITIAN

Tanggal Persiapan (pengumpulan sampel) Januari 2010 – Juni 2013

Pelaksanaan 15 Juni 2013

Penyusunan Laporan 15 Juli 2012

Penggandaan Laporan 18 Juli 2012

PROSEDUR PEMERIKSAAN IMUNOHISTOKIMIA

PROTEIN S 100

NO GAMBAR KETERANGAN

1

Panaskan slide hasil potongan microtome pada hotplate suhu 60 °C selama 60 menit

2

Xylene 2 kali selama 2 menit

3

Dehidrasi (Alkohol absolut, 80%, 70%) selama 2 menit

4

Bilas dengan air mengalir (keran) selama 2 menit

5

Akuades selama 5 menit

6

Masukkan ke dalam Microwave Samsung TDS dengan kondisi sbb: 800 watt : 2,5-3 menit 100 watt : 10 menit

7

Dinginkan slide selama 20 menit

8

Bilas dengan Wash Buffer (WB)/PBS-T selama 5 menit

9

Blocking dengan DAKO FLEX Peroxidase selama 5 menit

10

Bilas dengan Wash Buffer (WB) /PBS-T selama 5 menit

11

12

Pemberian Protein S100 dan di diamkan selama 20-60 menit Bilas dengan Wash Buffer (WB)/PBS-T selama 5 menit

13

Tetesi denga flek HRP

14

Bilas dengan Wash Buffer (WB)/PBS-T selama 5 menit

15

Pemberian FLEX DAB + SUBSTRAT didiamkan selama 5 menit

16

Pemberian Hematoxylin dan didiamkan selama 2 menit

17

Dehidrasi (Alkohol 70%, 80%, Absolut) selama 5 menit

18

Pengamatan di bawah mikroskop

Hasil pemeriksaan IHC Protein S100

1. Jumiyan, Pr, 52th No. PA : B / 3203 / 10

Histopatologi IHC Protein S100 Kesimpulan : +++

2. Dut Wan Minah, Pr, 47th

No. PA : O / 2750 / 10

Histopatologi IHC Protein S100 Kesimpulan : +++

3. Yulia Safitri, Pr, 40th No. PA : B / 3062 / 10

Histopatologi IHC Protein S100 Kesimpulan : +++

4. Yusnijar, Pr, 53th No. PA : O / 3243 / 10

Histopatologi IHC Protein S100 Kesimpulan : +++

5. Syafrizal Pulungan, Lk, 43th No. PA : B / 6410 / 10

Histopatplogi IHC Protein S100 Kesimpulan : +

6. Maakdin, Lk, 60th No. PA : O / 868 / 10

Histopatologi IHC Protein S100 Kesimpulan : +

7. Pelki Tawa, Pr, 63th No. PA : O / 2397 / 10

Histopatologi IHC Protein S100 Kesimpulan : -

8. Suwito, Lk, 43th No. PA : 8251

Histopatologi IHC Protein S100 Kesimpulan : +++

9. Nelly, Pr, 67th No. PA : 7649

Histopatologi IHC Protein S100 Kesimpulan : +++

10. Yusli, Lk, 45th No. PA : 8342

Histopatologi IHC Protein S100 Kesimpulan : ++

11. Jintaman Purba, Pr, 54 th No. PA : B / 429 / 11

Histopatologi IHC Protein S100 Kesimpulan : +++

12. Indah Pratiwi, Pr, 40th No. PA : H 223 03 12

Histopatologi IHC Protein S 100 Kesimpulan : +++

13. Nurmaini, Pr, 52th No. PA : H 813 08 12

Histopatologi IHC Protein S100 Kesimpulan : +++

14. Andro, Lk, 40 th No. PA : H 905 09 12

Histopatologi IHC Protein S100 Kesimpulan : +++

15. Andayani, Pr, 67th No. PA : H 114 02 12

Histopatologi IHC Protein S100 Kesimpulan : +

16. Rosmawati, Pr, $0th No. PA : OS.001.06.13

Histopatologi IHC Protein S100 Kesimpulan : +++

17. Juli, Pr, 44th No. PA : 8869

Histopatologgi IHC Protein S100 Kesimpulan : +++