BAB V KE SIMPULAN DAN SARAN
B. Saran
1. Perlu dilakukan penelitian aktivitas antioksidan dari fraksi air ekstrak etanolik daun dadap serep untuk melihat potensi aktivitas antioksidan dari fraksi air tersebut.
2. Perlu dilakukan penelitian untuk melihat potensi aktivitas lainnya (seperti aktivitas antibakteri) dari daun tanaman dadap serep.
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LAMPIRAN
Lampiran 2. Gambar tanaman dadap serep dari kebun tanaman obat Fakultas Farmasi Universitas Sanata Dharma
Lampiran 3. Perhitungan rendemen Cawan1 (g) Cawan 2 (g) Cawan 3 (g) Bobot cawan 59,1322 59,4312 59,2913 Bobot cawan+ekstrak 64,3125 63,7355 64,0414 Bobot ekstrak 5,1803 4,3043 4,7501
Total bobot ekstrak 14,2347
Bobot serbuk daun dadap yang digunakan = 100 g Bobot total ekstrak etanol = 14,2347 g
Rendemen ekstrak etanol = �
� � x 100%
= 14,2347
100 x 100%
= 14,2347 %
Bobot fraksi etil asetat = 0,4238 g
Rendemen fraksi etil asetat = � �
� � x 100%
= 0,4238
100 x 100% = 0,4238 %
Lampiran 4. Data penimbangan untuk pengujian aktivitas antioksidan a. Penimbangan DPPH
Replikasi 1 (g) Repilkasi 2 (g) Replikasi 3 (g)
Bobot kertas 0,2123 0,2129 0,2130
Bobot kertas + DPPH 0,2279 0,2286 0,2289
Bobot kertas + sisa 0,2123 0,2129 0,2130
Bobot DPPH 0,0156 0,0157 0,0159 b. Penimbangan Kuersetin Replikasi 1 (g) Repilkasi 2 (g) Replikasi 3 (g) Bobot kertas 0,2347 0,2326 0,2439
Bobot kertas + kuersetin 0,2373 0,2352 0,2465
Bobot kertas + sisa 0,2348 0,2327 0,2439
Bobot zat 0,0025 0,0025 0,0026
c. Penimbangan sampel uji
Replikasi 1 (g) Replikasi 2 (g) Replikasi 3 (g)
Bobot cawan 14,5897 14,114 14,1086
Bobot cawan + fraksi 14,6025 14,1266 14,1211
Bobot cawan + sisa 14,5899 14,1141 14,1086
Lampiran 5. Data perhitungan konsentrasi pengujian aktivitas antioksidan a. Konsentrasi DPPH Replikasi 1 BM = 394,33 Mol = massa BM = 15,6 mg 394 ,33 = 0,0395 mmol M = mol volume = 0,0395 mmol 0,1 L = 0,395 mM
