SIMPULAN DAN SARAN
7.1 Simpulan
Berdasarkan hasil penelitian, maka dapat disimpulkan :
1. Ekstrak daun kelor ( Moringa oleifera) dengan pelarut air dengan sangat
nyata dapat menghambat aktivitas bakteri E. coli patogen.
2. Ekstrak daun kelor ( Moringa oleifera) dengan pelarut etanol dengan
sangat nyata dapat menghambat aktivitas bakteri E. coli patogen.
3. Ekstrak daun kelor ( Moringa oleifera) dengan pelarut air pada penelitian
ini memiliki daya hambat optimum pada konsentrasi 50% (8.3 ± 3.1544)
mm, sedangkan ekstrak daun kelor ( Moringa oleifera) dengan pelarut
etanol pada penelitian ini memiliki daya hambat optimum pada
konsentrasi 75% (14 ± 1.0000) mm.
7.2 Saran
Perlu dilakukan penelitian lebih lanjut mengenai bioaktifitas ekstrak daun
kelor ( Moringa oleifera) secara in vivo pada babi penderita kolibasilosis dan
pemberian daun kelor ( Moringa oleifera) sebagai campuran pakan untuk
memaksimalkan pemanfaatan daun kelor ( Moringa oleifera) sebagai alternatif
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LAMPIRAN
Lampiran 1. Tabel Rata-rata zona hambat ekstrakdaun kelor ( Moringa oleifera) dengan pelarut air
Descriptive Statistics Dependent Variable:air
perlakuan Mean Std. Deviation N
pengenceran 25% 7.700 1.0368 5 pengenceran 50% 8.300 3.1544 5 pengenceran 75% 6.000 4.3875 5 pegenceran 100% 1.900 2.8810 5 kontrol - .000 .0000 5 kontrol + 24.700 1.6047 5 Total 8.100 8.4806 30
Lampiran 2. Analisis Sidik Ragam ekstrak daun kelor ( Moringa oleifera) dengan pelarut air
Tests of Between-Subjects Effects Dependent Variable:air
Source
Type III Sum of
Squares df Mean Square F Sig.
Corrected Model 1921.100a 5 384.220 56.022 .000 Intercept 1968.300 1 1968.300 286.994 .000 perlakuan 1921.100 5 384.220 56.022 .000 Error 164.600 24 6.858 Total 4054.000 30 Corrected Total 2085.700 29
Lampiran 3. Uji LSD ekstrak daun kelor ( Moringa oleifera) dengan pelarut air
Multiple Comparisons Dependent Variable:air
(I) perlakuan (J) perlakuan
Mean Difference
(I-J) Std. Error Sig.
95% Confidence Interval
Lower Bound Upper Bound
LSD pengenceran 25% pengenceran 50% -.6000 1.6563 .720 -4.018 2.818 pengenceran 75% 1.7000 1.6563 .315 -1.718 5.118 pegenceran 100% 5.8000* 1.6563 .002 2.382 9.218 kontrol - 7.7000* 1.6563 .000 4.282 11.118 kontrol + -17.0000* 1.6563 .000 -20.418 -13.582 pengenceran 50% pengenceran 25% .6000 1.6563 .720 -2.818 4.018 pengenceran 75% 2.3000 1.6563 .178 -1.118 5.718 pegenceran 100% 6.4000* 1.6563 .001 2.982 9.818 kontrol - 8.3000* 1.6563 .000 4.882 11.718 kontrol + -16.4000* 1.6563 .000 -19.818 -12.982 pengenceran 75% pengenceran 25% -1.7000 1.6563 .315 -5.118 1.718 pengenceran 50% -2.3000 1.6563 .178 -5.718 1.118 pegenceran 100% 4.1000* 1.6563 .021 .682 7.518 kontrol - 6.0000* 1.6563 .001 2.582 9.418 kontrol + -18.7000* 1.6563 .000 -22.118 -15.282 pegenceran 100% pengenceran 25% -5.8000* 1.6563 .002 -9.218 -2.382 pengenceran 50% -6.4000* 1.6563 .001 -9.818 -2.982 pengenceran 75% -4.1000* 1.6563 .021 -7.518 -.682 kontrol - 1.9000 1.6563 .263 -1.518 5.318 kontrol + -22.8000* 1.6563 .000 -26.218 -19.382 kontrol - pengenceran 25% -7.7000* 1.6563 .000 -11.118 -4.282 pengenceran 50% -8.3000* 1.6563 .000 -11.718 -4.882 pengenceran 75% -6.0000* 1.6563 .001 -9.418 -2.582
pegenceran 100% -1.9000 1.6563 .263 -5.318 1.518 kontrol + -24.7000* 1.6563 .000 -28.118 -21.282 kontrol + pengenceran 25% 17.0000* 1.6563 .000 13.582 20.418 pengenceran 50% 16.4000* 1.6563 .000 12.982 19.818 pengenceran 75% 18.7000* 1.6563 .000 15.282 22.118 pegenceran 100% 22.8000* 1.6563 .000 19.382 26.218 kontrol - 24.7000* 1.6563 .000 21.282 28.118
Lampiran 4. Tabel Rata-rata zona hambat ekstrak daun kelor ( Moringa oleifera) dengan pelarut etanol
Descriptive Statistics Dependent Variable:etanol
perlakuan Mean Std. Deviation N
pengenceran 25% 9.600 .4183 5 pengenceran 50% 12.600 .7416 5 pengenceran 75% 14.000 1.0000 5 pegenceran 100% .600 1.3416 5 kontrol - .000 .0000 5 kontrol + 23.800 .7583 5 Total 10.100 8.3557 30
Lampiran 5. Analisis Sidik Ragam ekstrak daun kelor ( Moringa oleifera) dengan pelarut etanol
Tests of Between-Subjects Effects Dependent Variable:etanol
Source
Type III Sum of
Squares df Mean Square F Sig.
Corrected Model 2008.300a 5 401.660 587.795 .000 Intercept 3060.300 1 3060.300 4478.488 .000 perlakuan 2008.300 5 401.660 587.795 .000 Error 16.400 24 .683 Total 5085.000 30 Corrected Total 2024.700 29
Lampiran 6. Uji LSD ekstrak daun kelor ( Moringa oleifera) dengan pelarut etanol
Multiple Comparisons Dependent Variable:etanol
(I) perlakuan (J) perlakuan
Mean Difference
(I-J) Std. Error Sig.
95% Confidence Interval
Lower Bound Upper Bound
LSD pengenceran 25% pengenceran 50% -3.0000* .5228 .000 -4.079 -1.921 pengenceran 75% -4.4000* .5228 .000 -5.479 -3.321 pegenceran 100% 9.0000* .5228 .000 7.921 10.079 kontrol - 9.6000* .5228 .000 8.521 10.679 kontrol + -14.2000* .5228 .000 -15.279 -13.121 pengenceran 50% pengenceran 25% 3.0000* .5228 .000 1.921 4.079 pengenceran 75% -1.4000* .5228 .013 -2.479 -.321 pegenceran 100% 12.0000* .5228 .000 10.921 13.079 kontrol - 12.6000* .5228 .000 11.521 13.679 kontrol + -11.2000* .5228 .000 -12.279 -10.121 pengenceran 75% pengenceran 25% 4.4000* .5228 .000 3.321 5.479 pengenceran 50% 1.4000* .5228 .013 .321 2.479 pegenceran 100% 13.4000* .5228 .000 12.321 14.479 kontrol - 14.0000* .5228 .000 12.921 15.079 kontrol + -9.8000* .5228 .000 -10.879 -8.721 pegenceran 100% pengenceran 25% -9.0000* .5228 .000 -10.079 -7.921 pengenceran 50% -12.0000* .5228 .000 -13.079 -10.921 pengenceran 75% -13.4000* .5228 .000 -14.479 -12.321 kontrol - .6000 .5228 .262 -.479 1.679 kontrol + -23.2000* .5228 .000 -24.279 -22.121 kontrol - pengenceran 25% -9.6000* .5228 .000 -10.679 -8.521 pengenceran 50% -12.6000* .5228 .000 -13.679 -11.521 pengenceran 75% -14.0000* .5228 .000 -15.079 -12.921
pegenceran 100% -.6000 .5228 .262 -1.679 .479 kontrol + -23.8000* .5228 .000 -24.879 -22.721 kontrol + pengenceran 25% 14.2000* .5228 .000 13.121 15.279 pengenceran 50% 11.2000* .5228 .000 10.121 12.279 pengenceran 75% 9.8000* .5228 .000 8.721 10.879 pegenceran 100% 23.2000* .5228 .000 22.121 24.279 kontrol - 23.8000* .5228 .000 22.721 24.879
Proses Pembuatan Ekstrak daun kelor ( Moringa oleifera)
Penimbangan daun kelor kering Perendaman daun kering 1-2 hari
Isolasi E.coli patogen
Isolat lapangan E.coli dalam media transport amies
Penanaman pada media Mc conkey agar
Hasil Penanaman pada media Mc conkey agar
Hasil penanaman bakteri pada media SBA (Sheep Blood Agar )
Hasil Uji Hambat Ekstrak air daun kelor ( Moringa oleifera)
E.coli patogen 1 E.coli patogen 2
E.coli patogen 3 E.coli patogen 4