Simpulan
Metode analisis simultan Cr(III) dan Cr(VI) dengan reagen kromogenik campuran (2-hidroksibenzaldiminoglisina dan kuersetin) menggunakan HPSAM pada kondisi optimum analisis yang diperoleh masih belum menunjukkan hasil yang baik. Hal ini ditunjukkan dengan %SBR > 5% untuk Cr(VI) dan tidak terdeteksinya Cr(III) pada contoh sintetik yang digunakan. Walaupun demikian HPSAM ini potensial untuk dikembangkan lebih lanjut dalam penentuan simultan Cr(III) dan Cr(VI) karena dalam beberapa ulangan pengukuran masih memberikan hasil yang cukup baik untuk Cr(VI) dengan adanya spesi pengganggu Cr(III). Selain itu, reagen kromogenik yang digunakan untuk pengukuran individu maupun dengan adanya gangguan dari spesi nontarget memberikan kelinearan kurva kalibrasi cukup baik dan estimasi limit deteksi yang cukup rendah.
Saran
Kondisi pembentukan kompleks kedua ion masih perlu untuk dioptimumkan agar dapat memberikan hasil yang teliti dan akurat saat digunakan HPSAM untuk pengukuran kuantitatifnya. Pembuatan reagen 2-hidroksibenzaldiminoglisina masih perlu ditelaah lebih lanjut agar memberikan keterulangan dan ketertiruan yang baik saat membentuk kompleks dengan Cr(III). Selain itu pula perlu dipelajari mekanisme pembentukan kompleks Cr(III)-2-hidroksibenzaldiminoglisina agar dapat diperoleh informasi karakteristik kompleks yang terbentuk sehingga akan lebih memudahkan dalam menentukan kondisi analisis simultan dengan Cr(VI) yang optimum.
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49 Lampiran 1 Spektrum absorpsi Cr(III)-2-hidroksibenzaldiminoglisina (a) tanpa
CTAB dan (b) dengan CTAB
Abs
400 500 600 700 nm
0.00
0.05
0.10
0.15
0.20
0.25
0.30
0.35
0.40
0.45
0.50
0.55
a bLampiran 2 Pengaruh jenis dan konsentrasi bufer terhadap nilai absorbans kompleks Cr(VI)-kuersetin dan Cr(III)-2-hidroksibenzaldiminoglisina Abs 450 500 550 600nm -0.1 0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1.0 1.1
Spektrum absorpsi Cr(VI)-Kuersetin dengan CTAB dan buffer fosfat 0,01 M pada berbagai waktu (Abs tertinggi pada λ maks diperoleh pada menit ke-20) Abs 450 500 550 nm 0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
Spektrum absorpsi Cr(VI)-Kuersetin dengan CTAB dan buffer asetat 0,01 M pada berbagai waktu (Abs tertinggi pada λ maks diperoleh pada menit ke-25)
51 Lanjutan Abs 450 500 550 600nm 0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1.0
Spektrum absorpsi Cr(VI)-Kuersetin dengan CTAB dan buffer fosfat 0,1 M pada berbagai waktu (Abs tertinggi pada λ maks diperoleh pada menit ke-20)
Abs 450 500 550 600 650 nm 0.00 0.05 0.10 0.15 0.20 0.25 0.30 0.35 0.40 0.45 0.50
Spektrum absorpsi Cr(III)- 2-hidroksibenzaldiminoglisina dengan buffer fosfat 0,01 M pada berbagai waktu (Abs tertinggi pada λ maks diperoleh pada menit ke-30)
Lanjutan Abs 450 500 550 600 nm 0.00 0.05 0.10 0.15 0.20 0.25 0.30 0.35 0.40
Spektrum absorpsi Cr(III)- 2-hidroksibenzaldiminoglisina dengan buffer asetat 0,01 M pada berbagai waktu (Abs tertinggi pada λ maks diperoleh pada menit ke-35) Abs 400 450 500 550 600 nm 0.00 0.05 0.10 0.15 0.20 0.25 0.30 0.35 0.40 0.45 0.50 0.55
Spektrum absorpsi Cr(III)- 2-hidroksibenzaldiminoglisina dengan buffer fosfat 0,1 M pada berbagai waktu (Abs tertinggi pada λ maks diperoleh pada menit ke-5)
53 Lampiran 3 Plot HPSAM untuk analisis kuantitatif simultan Cr(III) dan Cr(VI);
(a) Cr(III) = 6.00 µg/ml dan Cr(VI) = 0.10 µg/ml; (b) Cr(III) = 6.00 µg/ml dan Cr(VI) = 0.20 µg/ml; dan (c) Cr(III) = 6.00 µg/ml dan Cr(VI) = 0.30 µg/ml yang diukur pada panjang gelombang 558 dan 577 nm
[Cr(VI)] yang ditambah (μg/ml) a
b c
