Simpulan
Hasil identifikasi bakteri potensial yang diperoleh adalah Pseudomonas aeruginosa dan Bacillus amyloliquefaciens JR02. Sejumlah fragmen DNA kromosom B. amyloliquefaciens JR02 berhasil di kloning pada vektor pRK415. Total 991 koloni transforman TcR yang diperoleh belum menunjukkan ekspresi gen antifungi. Disisi lain pada saat yang bersamaan, karakterisasi DNA pengapit mutan BglK226 menunjukkan tingkat kemiripan 99% dengan sebagian gen beta-ketoacyl synthase.
Saran
Perlu dilakukan penelitian lebih lanjut mengenai verifikasi gen beta-ketoacyl synthase pada isolat tipe liar.
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LAMPIRAN
Lampiran 1 Pembuatan sel kompeten dengan perlakuan CaCl2(Sambrook et al. 1989)
Sebanyak 2 mL biakan E.coli TOP10 umur 12 jam dikulturkan ke dalam 40 mL media LB. Kultur diinkubasi selama 2-3 jam pada suhu 37 °C sampai nilai Optical Density (OD600) mencapai 0.45-0.5. Selanjutnya kultur diambil sebanyak 1,5 mL dan disentrifugasi pada kecepatan 5000 rpm selama 15 menit. Pelet diresuspensi dengan 1 mL bufer transformasi. Sampel diinkubasi di dalam es selama 15 sampai 30 menit, kemudian disentrifugasi pada 5000 rpm selama 5 menit pada suhu 4 °C. Pelet diresuspensi kembali dengan 250 µL bufer transformasi dan disimpan dalam es.
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