Simpulan
Dari hasil penelitian dapat diketahui bahwa ekstrak sayuran indigenotts merupakan antioksidan yang dapat berfungsi sebagai radial scavenger, mempunyai kemampuan mereduksi dan dapat menghambat terjadinya oksidasi lipid lanjut.
Berdasarkan hasil analisis data mengenai hubungan nilai total fenol dengan aktivitas antioksidan ekstrak sayuran indigenous dapat diketahui bahwa nilai total fenol secara keseluruhan berpengaruh terhadap kapasitas antioksidan ekstrak sayuran indigenous. Semakin tinggi nilai total fenol ekstak antioksidan maka semakin tinggi kemampuannya sebagai radikal scavenger, semakin tinggi kemampuan mered~ksinya dan semakii tinggi kemampuannya dalam menghambat terjadinya oksidasi lipid lanjut.
Semakin tinggi kemampuan mereduksi dari suatu antioksidan maka semakin tinggi kapasitas antioksidannya yang ditandai dengan semakin tinggi kemampuannya dalam menekan perkembangan radikal bebas.
Kemampuan mereduksi dari suatu antioksidan dipengamhi oleh kandungan flavonol dan flavon yang terdapat pada ekstrak antioksidan tersehut.
Saran
Disarankan untuk melakukan uji lanjut kapasitas antioksidan secara in vivo untuk mengetahui efektivitas ekstrak antioksidan sayuran indigenous dalam menekan perkembangan radikal bebas.
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b l L + 18568
Lampiran 3 Hasil pengukuran kadar air bubuk kering berbagai sayuran
Lampiran 4. Hasil pengukuran kadar bahan kering ekstrak sayuran Indigenous
Lampiran 5. Hasil pengukuran rendemen ekstrak sayuran indigenoirs
Lampiran 6 Hasil pengukuran nilai total fenol ekstrak sayuran indigenous
[Galic acid]
( m g l ~ )
0.173 0.382 0.622
200 0.824
1.087
300 1.318
100 200 300
Kons. Galic Acid (mglL)
0
0 100 200 300 400
j
Kons. Galic A c ~ d (mglL)Lampiran 8 Hasil pengukuran kemampuan mereduksi sayuran indigenous
75 Lampiran 9 Hasi! pengukuran aktivitas antioksidan ekstrak sayuran indigenous metode DPPH
78
Lampiran 12 Hasil pengukuran kapasitas antioksidan berbagai jenis sayuran indigenous sebagai penghambat oksidasi lipid
j 0 1
0.00 0.50 1.00 1.50 2.00 2.50 3.00 3.50
1
j
Konsentrasi T M P (mM)Konsentrasi (uM)
0.50 I .OO 1.50 2.00 2.50 3.00
% Inhibisi =
.
[MDA Blanko] - [MDA sar x 100 % [MDA Blanko]Rata-rata 0.013 0.033 0.050 0.080 0.094 0.124 Absorbansi
I 0.012 0.035 0.049 0.078 0.095 0.128
2 0.014 0.03 1 0.05 1 0.08 1 0.092 0.12
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Lampiran 15 Grafik hubungan nilai total fenol dengan kemampuan mereduksi
0 20 40 60 80 100 120 140 160
82
Lampiran 16 Grafik hubungan nilai total fenol dan kemampuan penghambatan oksidasi lipid lanjut
! 0 20 40 60 80 100 I 2 0 140 160
I Nilai Total Fenol (ug GAEImg ekstrak)
!
Ekshak sampel
-
BeluntasKenikir Mangkokan
Keinangi Pohpohan Ginseng Kedondong Cina
B. Kecombrang Krokot
Total fenol (ug GAEImg ekstrak)
141.1 119.53
62.92 91.56 70.85 57.78 56.6 64.58 42.24
Penghambatan Pembentukan MDA (%)
98.55 98.15 97.70 97.04 97.26 97.13 97.06 96.55 96.02
83 Lampiran 17 Grafik hubungan kemampurtn mereduksi dengan kapasitaq antioksidan
menggunakan radikal DPPH
Ekstrak sampel
Lampiran 20 Hasil pengujian identifikasildeterminasi sayuran indigenous
LEMBAGA lbMU PENGETAHUAN INDONESIA
Perihal : Hasil identifikasi/deteminasi Tumbuhan
Kewada Yth.
~ & . / ~ b u / ~ d r ( i ) . Diny Agustini S.S.,ST.
Mhs.IPB
Dengan hormat,
Bersama ini kami sampaikan hasil identifikasildeterminasi tumbuhan yang S a u d a r a kirimkan ke "Herbarium Bogoriense", Bidang Botani Pusat Penelitian Biologi-LIP1 Bogor, adalah sebagai berikut :
Suku
No.
2
Daun Kernangi Daun Ginseng
( (
Daun Mangkokan(
Nothopanax scutellarius (Burm.f.) Merr. AraliaceaeDemikian, semoga berguna bagi Saudara.
No.
Kol.Daun Kenikir
~ a u n ~ o l ~ p o h a n
Daun Katuk
I
Sauropos androgynus (L.) Merr.Kembang Onje
(
Etlingera elatior (Jack) R.M.Sm.Kep2lk;~idang