Simpulan
Berdasarkan hasil penelitian dapat disimpulkan bahwa penambahan FBS dan BSA mampu mendukung tingkat maturasi dan tingkat fertilisasi yang lebih baik dibandingkan dengan kelompok PVA dan Kontrol.
Saran
Perlu dilakukan penelitian lebih lanjut hingga tahap kultur embrio untuk melihat pengaruh penambahan serum atau komponennya (BSA), dan makromolekul PVA pada tahap lebih lanjut.
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Lampiran 1 Komposisi media transportasi ovarium
Komponen Jumlah
Sodium chloride (Sigma Aldrich, USA) 9.0 g
Milli Q water 1000 mL
Penicilin-streptomycin (100IU/mL) 1000 μL Lampiran 2 Komposisi media koleksi
Komponen Jumlah
PBS 100 mL
Penicilin-streptomycin (100 IU/mL) 1000 μL Fetal Bovine Serum 10% (v/v) mL
Stok PBS : 9.6 g (Dulbecco’s Nissui, Japan) / 1000 mL milli - Q water Stok penicillin-streptomycin
Dosis penicillin: 0.06 g/L = 100.000 IU/L Dosis streptomycin :0.1 g/mL
Untuk membuat stok Penicillin 0.0006 g (Sigma-Aldrich. Inc, P-4687) dan streptomycin (Sigma-Aldrich. Inc, S-9137) 0.001 g dilarutkan dalam 10 mL milli-Q water.
Fetal Bovine Serum 10% (v/v) mL (untuk stok 50 mL PBS, 5 mL FBS yang digunakan)
Lampiran 3 Komposisi media dasar TCM-199 untuk maturasi oosit
Komponen Jumlah Larutan TCM-199 5 mL PMSG 50 μL hCG 50 μL Gentamycin 5 μL Stok PMSG
Untuk membuat stok, maka 1000 IU PMSG dilarutkan dalam 1 mL TCM 199 Stok hCG
25 Lampiran 4 Komposisi media fertilisasi in vitro
Komponen mM mg/100 mL NaCl 90 525.96 KCl 12 89.46 NaHCO3 25 210.03 NaH2PO4 anhydrous 0.5 6.00 MgSO4 7H2O 0.5 12.33 Sodium lactat 10 112.10 60% syrup 0.19 mL Hepes 10 238.30 CaCl2 2H20 8 117.60 Sodium piruvat 2 22.00 Caffeine anhydrous 2 38.44
BSA (Fatty acid free) 5 mg/mL 500.00
Suzuki et al. (2000). Effect of hyaluron on monospermic penetration of procine oocyte fertilized in vitro. Int J Androl 23:13-21.
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