The effect of lipid on the digestion of cellulose by the ruminant : a thesis presented in partial fulfilment of the requirements for the degree of Master of Agricultural Science in Animal Science at Massey University

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'1'tlE EPJ?.mT OP LIPID OH mE DIGEMI<ll OP cm~DLOSI: BY tm!

ammwn.

A thctai• preaouiecl in pe.rtJ.al tulltlt.lcmt ~ tho roquiromonta

toz

t.ho deta;reo

ot

Maetor of Agricultural Sci4meo in 4niml Science at Maaoy Univerai:v.

I vish 1to tho.Dk tho follo in.g who gave val bl in varloua "*'ll.Y :

siatanc

Dr. J.C. Jta'Wko for th guJ.clanc alld holptul &dvice given throuchout the coure of this vo

Prof aor D.S. Plux anc1 diaouaai and ^c~1~1ol

• G.P. Vtlaon for thrir ot this

•

uable

Tb Ch 'Z'Y and Dioc a ry Department tor the proviaion ot o.p}.)U'Gt and t ilitles d.urin8 1969.

Mr. .A. Tho on and •• J.M. Alcl raloy for t clmiCGl aaaistancei and 'the et.af'f of No. 2 Dairy Unit for the o e of th fiatulated c t'tle •

• o .J .

land for yping t e ti 1 copioa

ot

th th aia.

vtte,

ial euppo

, tor

•

1. bol 2. lntorc 3. P:repl 4.

5.

6. ln-vi:tro

OP

OP CONTENTS

of Lipicla by ta ion ot Individual Vola~tle

t

~

tu

t 13 13 t4 17 19 24

1. Preliminary Experiments

..,

""-·•

1.1. Gas Production 1.2 Ammonia Production

1.3 Total and individual VFA production

t:ff ect of different rnounts of lipid substra"te on total and

individual VPA production and o.mmoni production fr grass

p ge 40

40 42 42

fibre incubated in rumen liquor for 24, 36, 48, or 72hr. '15 2.1 Total VFA production

2.2 Acetate production 2.3 Propiona.t production 2.4 llutyr te production 2. 5 onia production

2.6 pH d terminntion of rumen liquor incub tions cont.aining grass fibre and linseed oil vithin iopermenblo apparatus

3. The effect of variou lipids on volntile fatty a.cid production.

3.1 Totnl &nd individual VPA production from rumen

46 47 48 49 51

52

56

liquor containing r s tibro. 56

4.

3.2 Comparison of the offect of lipid upon ~he

production of 'tot.al o.nd individu l VPA's of en liquor incub d vi.th or without s fibre. The use of 14

c-colluloao in rumen fermentations of lipid M1d gro.ss fibre vithin · :po eable apparatus.

4.1 Total ood individual. producti 4.2 Incorpo tion ot ¹⁴

c

into ~

co

2 4.3 Di tribution

o~

¹⁴

c

individ 1 VPA'a 4.4 Bet production of to~ 1 VPA, ac tie, propionic

butyric acid •

4.5 Incorporntion ot ¹⁴

c

into ^{~ and}

co

₂

4.6 Distribution of 14

c

individual VPA'

59

61

61 62 62

64 65 65

6.

1.

Th inc :tton of (2-14

c)

lyeerol

(o. 4;'-

)IC•/: • tn

~

^...

-~

lictuor vi'th gna: fibre and cod liver oil.

5.2

tic. prepicm.to, n.nd bu\yric acids.

Th rcon incorpora'tion ot 14 C from (2-14

c)glyeerol inW total and individual VPA'a.

ta

uaina

^{the ·} cro-art.Uicial fl

ot lipid

ruriien t·enllelltati

·ra..A~ltiei 1 »tmana.

68

68

70

72 72 75

83

7.1 fo

VPA

p l 83

1. 2

Toto.l and individual 14

c-;

~

procludion 84

7.3 Inv: irtiga'tiona in'to e adsmlpti and carryover

of VPA' a on to p1\v col • 86

DISCUSSIC»l 89

VPA • •·

Use of Micro-Artifici

•

•

89 91

92

)

96

98

'°'

104 107

LIST OP PI<@.!l@S

1

2 The off t of 11.~""' oil(2.0g. ) on lndirldual Vl'A coneent.rat.lona ( le/l. ) We.n

a.sg .

of grass fibre

43 3 The tfeet of 1insHd otl(O, 1. 2 3g./t00ml. ~- 1mtan liquor)

total and vi4ual VPJ. eancont. t.i ( l /1. )

o .Sg.

of tib inc ted in liquor

(50mL)

tor

24, 36• 48

or

"12br.

so

ettoct ~ 'Of tiOD on 'the ommu.a

e :tnticm ( /100J::tl. ) o~ liquor ( 50ml. ) 1ning 0 .. 5g •. of gnas tibr& 1 ed oil

(o,

1, 2

a

^lg./1~.) 53

5 The

ott&et

Gf 1· ed. oil{O, 1, 2

&

3g./100nl.

rumen

liq

or)

-on

om.a

eoncentra-tions (mg. /100m1.) imen 0.5g.

o.t

u:r&l!Hll fibre incubated in rm:mn liquor (50ml. ) for

24, 36, 48 or 12br.

6 Total VPA

pna

ion.(

lo/l. }

^{~l'OID rumen} llquor(50ml. ) i11eubate4 th ( ) 0.5a .. of fibre + 1.5g.

ot

cod li oilJ (b)

o . 5g.

^{~ a ti ; (e) or it.hon~}

1 'bstrate.. for 24lu'. thin e

~PDDl'Atua. ( .!.•) • T1

1

. )

2 >hr. ··K~ ~ ... l..D.

~~·w.,. {EXJm1am• _. ).-

9

10

8(t't;d-Jlleri:ooablo 4

tu

contciutng •

t

liquor ineu'batocl

tor

24br. ,nth (a) 0.5 • ai- tib"l'e

+

1.;g. of cod liver oilJ (b)

o. •

^of fibre; or (c) rlthout

ooM"

^~

•

^~

vi thin. tho a 1 ~ conte.ild.DS 5 .. of

1'0m8ll llquor !>a

ror

^24hr. d1h ( ) o.5 • ~ nrtM•

i + 1.Sg.

ot

^{u~ t11 (b)}

o. 5g.

o1 :rib •

or (c) tbmtt a.tldJ:ti

a .

81

Jll"OdUC'tiOD d t4 _f

l!'!lll:mn liquor

in

tho

ot (()) o .

(b) o . •

^o~ tibro +

1. 0g.

ol

li

PBlU fil>M;

oil; or (c)

0. 5g. of

gr tibro +

l

.

Og. of cod UTer oil for 2.fhr

..

thin the 801!11-;per~•bl apparatus. 85