Direct Cloning of a Xylanase Gene from Pawan-Riau Hot Spring
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The number of microorganisms rapidly increased and was comparable with the high activity of the xylanase produced. The value of enzyme activity produced by using auto- clave
A previous study successfully isolated and charac- terized the bcfd1 gene that encoded dehalogenase enzyme from Bacillus cereus IndB1 obtained from the Indonesian Agriculture
coli Top10 The purified of PCR product was cloned to the expression vector pET101/D-TOPO directly from the blunt end PCR product without restriction enzyme treatment
coli Top10 The purified of PCR product was cloned to the expression vector pET101/D-TOPO directly from the blunt end PCR product without restriction enzyme treatment
The aim of this research is to conduct the cloning of GH11 xylanase coding gene from Bacillus halodurans CM1 using pJET 1.2 / blunt plasmid vector into Escherichia coli DH5α as cell
Theoretical framework Figure 1 The theoretical framework of the study Starting from the disadvantages and difficulties of the current traditional hot spring business model, this
Box 9004, Abha 61413, Saudi Arabia Keywords: Mini-proinsulin, human insulin, Cloning and expression, Escherichia coli, Synthetic Gene.. INTRODUCTION Since the beginning of
Based on the results of the AHP analysis, the priority for the development of natural hot spring tourism in Hapanasan, Rokan Hulu Regency, Riau, is on the criteria for aspects of