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PERBANDINGAN DAYA HAMBAT BERBAGAI METODE REBUSAN DAN PERASAN DAUN SAMBILOTO (Andrographis paniculata, Ness) PADA PERTUMBUHAN Shigella dysenteriae SECARA In vitro

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PERBANDINGAN DAYA HAMBAT BERBAGAI METODE REBUSAN DAN

PERASAN DAUN SAMBILOTO (Andrographis paniculata, Ness) PADA

PERTUMBUHAN Shigella dysenteriae SECARA In vitro

Oleh: TAUFIQ BUDIMAN ( 04330018 )

BIOLOGY

Dibuat: 2009-10-08 , dengan 3 file(s).

Keywords: Rebusan, Perasan, Daun Sambiloto, Antibakteri, Shigella dysenteriae

ABSTRAC

Sambiloto is the plants many known by the people and used as medicine

for various diseases, include dysentery disease was caused by Shigella dysenteriae bacteria. Based on the active essence content, sambiloto leaf contain tannin and flavonoid compound useful for anti bacteria substance. In general, Sambiloto leaf process was conducted the people by poached and pressed, so that it could be different effectively level to allay dysentery disease.

The research was conducted to find out resistivity of stew and distillation of sambiloto leaf toward the growth of Shigella dysenteriae and to find out

comparison of stew and distillation resistivity of sambiloto leaf toward the growth of Shigella dysenteriae.

This research was conducted for June 8th to July 2nd 2009 in the Bio

Technology Laboratory Muhammadiyah University of Malang. The research type of this research was true experiment and the experiment designed used was Completely Randomized Designed (RAL). The method used in this research is stew method R1 (25gr sambiloto + 900ml aquades), R2 (25gr sambiloto + 600ml aquades), R3 (25gr sambiloto + 300ml aquades) and the distillation method P1 (25gr sambiloto + 900ml aquades), P2 (25gr sambiloto + 600ml aquades), P3 (25gr sambiloto + 300ml aquades). The antibiotic of tetracycline as Kp control positive and K0 control negative without treatment, with three times repetation, so that sample unit used were 24 units. The parameter used to see resistivity of stew and distillation of sambiloto leaf was clear zone diameter at prolific Shigella

dysenteriae.

The analysis result of one way variant significant rate 1% indicated that

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The result of this research shown there are differenciate of influence dekok Phaleria macrocarpa (Scheff) Boerl) from other variant concentration on diameter inhibit zone

Efektivitas Bubuk Daun Jambu Biji ( Psidium guajava ), Daun Sirih ( Piper betle ) dan Daun Sambiloto ( Andrographis paniculata ) Untuk Pencegahan dan Pengobatan