September 1998, 73-78
ISSN 0854-8587
Vol. 5. No. 3
Uji Biopotensi Antibodi Monoklonal Anti
Pregnant Mare Serum
Gonadotropin
pada Sapi
(Biopotency Test of Monoclonal Antibody Anti Pregnant Mare Serum
Gonadotropin in Dairy Cattle)
IMAN TUTY L. BAMBANG PURWANTARA', G O Z A L I
DAN LIES PAREDE
'Bagian FKH IPB, Lodaya 16151
RI, Jalan R.E. No. 30, 16123
Diterima Mei 18 1998
The experiment was conducted to determine whether MoAb anti pregnant mare serum gonadotropin (PMSG) (PHB Product) possess neutralizing capacity and to determine an effective neutralizing biopotency against commercial PMSG used in dairy heifer cattle. Twenty five Fries Holland (FH) heifer donors were randomly divided into 5 groups. Group I as a negative control was superovulated with 2000 PMSG intramuscularly at PM. Donors in group IV, and V received the same treatment as those in group I, but were also administered with MoAb intravenously 30 minutes after PMSG injection. Group as a positive control received 3.3 commercial MoAb (Neutra PMSG), whereas groups IV, and V received ml; 1.0 and 2.0 MoAb (PHB Product), respectively. Biopotency test in dairy heifer donors, showed that 2000 IU PMSC used for superovulation was found to be not neutralized by in group and 1.0 of MoAb (PHB Product) in group Superovulation reaction observed were not different from that observed in group I donors (untreated with MoAb). However when the dose was increased to 2.0 superovulation reaction in treated donors group V were significantly different
from that observed in untreated donors. The result also showed that antibody treatments in group I1 (3.3 Neutra PMSC) or group V (2.0 PHB Product) could decreased significantly number of functional structure of ovaries and number of collected embryos. In conclusion, based on this finding MoAb (PHB Product) possess neutralizing capacity with the effective neutralizing biopotency to IU PMSG was estimated as 2.0
PENDAHULUAN
Dalam produksi sapi melalui program ovulasi, salah satu eksogen yang dapat dipakai lah pregnant mare serum gonadotropin (PMSG). pun potensial dalam stimulasi
tetapi hasil embrionya belum memuaskan. yang panjang dalam sirkulasi peredaran darah sapi 1 18- 123 jam Schams 1979) dan
pada babi masih terdeteksi sampai 170-248 jam (Ziecik Dybala 19%) memungkinkan untuk menginduksi
perkembangan secara ekstensif, tetapi ga efek samping yang negatif.
peneliti telah mencoba efek negatif di antaranya et (1978) melaporkan serum anti PMSG (biopotensi serum anti PMSG
2000 IU PMSG in vitro) pada superovulasi 2000-3000 IU PMSG meningkatkan angka ovulasi dari 9.4 sampai 15.7 dan folikel persisten dari 6.5 sampai 2.8, dengan peningkatan nisbah sel telur
dari 60% menjadi 80%. et al. (1980) telah bahwa pemberian serum anti PMSG dapat meningkatkan angka ova dan menekan
folikel yang ovulasi menekan
untuk Tel. 62-25 1-324358
gen yang berlebihan. Piper (1977)
kan bahwa antibodi eksogen terhadap PMSG dapat netralisir PMSG dan mereduksi waktu paruhnya pada ba dan sapi dapat memberikan hasil superovulasi
efek samping.
Dieleman et al. (1993) membandingkan waktu aplikasi yang berbeda-beda dari MoAb (biopotensi in 1 MoAb dapat 600 PMSG) untuk netralisasi
residu PMSG, puncak hormone (LH).
Berdasarkan data yang terkompilasi, corpus luteum (CL) yang dari 1 sampai 41 ngan hasil laik transfer masing-masing
an di antaranya fisiologi, 6 jam dan jam aplikasi pasca puncak LH 3.5, 4.1, dan 5.0. Aplikasi MoAb terhadap PMSG 18 jam pasca puncak LH
perbaikan nyata pada embrionya dingkan dengan grup yang mendapat fisiologi. rat MoAb mengandung imunoglobulin G yang
1994). Distribusi subkelas normal serum dengan waktu sekitar 2 1 hari 1987).
