Isolation and Molecular identification with 18SRDNA leaf Base Root Desease on Aloe barbadensis in Bali.

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Gonference Programs

sth

lnternational

Conference

on

Plant

Diversity

2015 (ICPD)

11th

Gongress on

lndonesian Society

of

Plant Taxonomy

Theme:

plant

Diversity:

A

Global

challenge

Graha WidYatama

U

niversitas Jenderal

Soedirman

Thursday'

Friday

20'21

August

2015

rlr

TU

! a- ..S' e

;e.+ "s - lnternational Conference On Plant Diverslty 2015

javanicum Miq, And 5. Aqueum _{( Burm.F. )} . Alston) Based

On Morpholosv Characteristic

14.30

-

14.4s Dewi Susan, Aohvllophorales Funei from Enesano lsland Graha Widvatama 05

14.45

-

15.00 Bambang Heliyanto, Fatkhur Rochman, Abdurrahman,

Collection Of Sugarcane Genetic Resources ln Sarmi Paoua

Graha Wiciyatama 01

14.45

-

15.00 Dewi Komariah, Alex Hartana, Morphological Variation Of

Kabau (Arhidendron bubalinum) ln Sumatra

Graha Widyatama 05

15.00- 1s.15 Coffea Break

Iime (WlB) Presenter of Session 2 Room

15.15

-

15.30 Sisunandar, Alkhikmah, Teguh Julianto, Capturing Genetic

Diversity For Ex Situ Conservation Of Kopyor Coconut ln Banyumas Areas

Graha Widyatama 01.

15.L5

-

15.30 Retno kawuri, lsolation And Molecular ldentification With

18s rDNA Leaf Base Rot Desease On Aloe Barbadensis ln

Bali

Graha Widyatama 05

15.30

-

15.45 Sarah Febriani, Characterization and Standarization of

Cashew cultivars (Anacardium occidentale L)

Graha Widyatama 01

15.30

-

15.45 Kartini Kramadibrata, Diversity of Arbuscular Fungi in

Batukau Nature Reserve and Mesehe Mountain. Bali

Graha Widyatama 05

15.4s

-

16.00 Murni Sapta Sari, Application of Project-Based Strategy on

Algae Diversity Material as an Attempt to lmprove Biology

Teacher Candidates Metacosnitive Skills

Graha Widyatama 01

15.45

-

16.00 Pudji Widodo, Four New Subspecies Of Syzygium

Samarangense (Myrtaceae) From lndonesia

Graha Widyatama 05

15.00

-

16.1s Niken Kusumarini, Diversity of Cubeb in Java Graha Widyatama 01

15.00

-

16.ls Endang Katikowati, Effect Of Combinations Various Plant

Spacing and Number of Seeds on Plant Growth and Rice

Yield Ciherang Varieties

Graha Widyatama 05

15.15

-

16.30 Tatik Chikmawati, Genetic diversity of wild rambutans

(Nephelium) in Sanggau, West Kalimantan based on SSR

and ISSR markers

Graha Widyatama 01

15.15

-

15.30 Merry Antralina, The effect of Bokashi Dosage on the

Growth of Arabica Coffee Seedling (Coffea arabica L.) Cultivar Andungsari l.

Graha Widyatama 05

16.30

-

15.45 Eko Sri Sulasmi, Spore Characters On Some Genus Of

Filicales Allies

Graha Widyatama 01

16.30

-

16.45 Suratman,Analysis of Genetic Variability in Avocado (Persea

americana Mill.) Population ln Central Java Based on Morphological and Molecular Characters

Graha Widyatama 05

16.45

-

17.00 Mariana, Feliatra Felix, Sukendi, Syafruddin Nasution,

Diversity of Mangrove Vegetation in Kuala tndragiri Coastal

Area Riau Province - lndoneisa

Graha Widyatama 01

L6.45

-

L7.OO Titien Ngatinem Praptosuwiryo, The Diversity of Ferns and

Fern Allies in Bogor Botanical Gardens

Graha Widyatama 05

17.00

-

17.15 Nurainas, Preliminary Study of the Genus Zingiber

(Zingiberaceae) in Sumatra

Graha Widyatama 01

L7.00

*

77.15 Nunik Sri Ariyanti, The genus Bazzania of Mount Sibayak,

North Sumatra

Graha WidVatama 05

17.t5

-

17.30 Wisnu Handoyo Ardi, Daniel Caspar Thomas, Mark

Hughes, Studies Begonia from Molucca lll: Reinstatement

of two Begonia muricata svnonVms

Graha Widvatama 01

*:;*-{f;*'=r

q:r: rlir _{* .r} ft

,**ii:f":, lnternational Conference On plant Diversity 2015

lsolation And

Morecurar

tdentification

with

_1gs

rDNA

On

Aloe

Barbadensis tn Bali

Retno Kawuri

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Leaf Base

Rot

Desease

Laboratory _{Microbiorogy Facurty Mathematic and science Udayana}

Em ail: microbiologylaboratory@yahoo.com ABSTRACT

Aloe vera plants (A.barbadensis _{Miller) has been planted in Bali since 2006 and spread out}

n five

regencies, i'e' _{Buleleng, Karangasem, Bangli, Gianyar and Badung; invotving}

13g farmers, with total area 170 hectare. Disease that h'ave _{oestroyeo Atoe vera}

L

_{reif base}

rol

_witn

the symptom

of rotten base leaf' The objectives of this researih were to know the occurrence of pathogen in

Bali and to identify the cauCal _{agent that responsible for the leaf}

base rot disease on atoe. survey

on

leaf base

rot

disease

in

Aloe vera piants

was

carrieJ

out

in

5

regencies

in

Bali, i.e.

Karangasem, Buleteng,. Bangli, Gianyar

and

Badung. eainogen isolation

on

sample was

conducted until pure culture _{was obtained, and then}

wal

_{inoculated on healthy Aloe vera} plants

to confirm that the same symptom occurred-

il

tlre _1en

irocrr

_{eostulate test). The causal agent}

was identified by molecular identification

of

_{18s rDNA. Results showed that the leaf base}

rot disease _{was distributed.in all five regencies in Bali}

wiih

"r;;rg;incidence

was 13.43% and the

causal agent of the leaf base rot disease was Fusaium

ir4"iZ*^.

&ar

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s;1r::ie::

!6;t'.:at:9 lnternational Conference On plant Diversity 20L5

Acknowledgements

we

have been supported by grant from Rector of Unsoed, _who has given us permission _{ot use} this building for free' Dean and Vice Deans of _{Fakultas Biologi Unsoed are thanked for support.}

we

also would like to thank Mr wisnu as the

donatur.

we

would like to _{thank prof. Mien A.}

Rifai'

and Prof'

Dr'

Dedy Darnaedi,

who

have

trust

us

to

be

the

committee

of the

_5th

lnternational conference on Plant Diversity lcPD 2015 at Unsoed

purwokerto.

prof _{Ferry slik} from the Herbarium of _{University Brunei Darussalam, Dr. Chandrakant Shalunke}

from Botany

lndia and Dr campbell webb _{(Harvard University Herbaria), are acknowledged for}

their willing

to

be

the

_{keynote speakers. special thank}

we

_due

to

vijayasankar Raman phD _{(Univ. of}

Mississippi usA) for _{critically reviewing some papers. I wourd like}

to

express _{my gratitude to}

Prof' Dr Estri Laras Arumingtyas _{for assistance and advice.}

we

_{are grateful}

to

Dr Titi Rugayah

and Mrs Yessi santika MSi who has _{coordinated this program from}

Bogor. we

also would tike

to _{thank all participants who have come to}

Purwokerto for your presentation. _,

,ro*i" .,

the

_{committee members}

of

the

_{lcPD 2015 who}

have worked hard

for the

success

of

this

conference'

Finally,

we

hope

that this

_{conference can increase}

our

awareness

on

_the importance

of

_{prant Diversity}

for our

better

future.

Furthermore,

we

;;;" ;;a

by

_this conference we can increase _{our collaboration amongst all participants and keynote}

speakers. Thank you.

Purwokerto,

August 20,2OlS

Committee

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