Test of Homogeneity of Variances

(1)

Lampiran 1

Analisis statistik menggunakan SPSS 16.0

a.

Data TPC bakteri pada senyawa hidrokarbon alifatik

NPar Tests

One-Sample Kolmogorov-Smirnov Test

ISOLAT WAKTU TPC

N 63 63 63

Normal Parametersa Mean 4.00 3.3333 8.3603

Std. Deviation 2.016 2.89047 2.89246

Most Extreme Differences Absolute .125 .231 .283

Positive .125 .213 .156

Negative -.125 -.231 -.283

Kolmogorov-Smirnov Z .993 1.834 2.248

Asymp. Sig. (2-tailed) .277 .002 .000

a. Test distribution is Normal.

Oneway

Test of Homogeneity of Variances

TPC

Levene Statistic df1 df2 Sig.

(2)

ANOVA

TPC

Sum of Squares df Mean Square F Sig.

Between Groups 367.064 6 61.177 22.592 .000

Within Groups 151.647 56 2.708

Total 518.711 62

Robust Tests of Equality of Means

TPC

Statistica df1 df2 Sig.

Brown-Forsythe 22.592 6 14.732 .000

a. Asymptotically F distributed.

Post Hoc Tests

Multiple Comparisons

TPC

Games-Howell

(I) ISOLAT

(J) ISOLAT

Mean Difference

(I-J) Std. Error Sig.

95% Confidence Interval

Lower Bound Upper Bound

OA2E1 SA1A .27778 .36772 .986 -.9888 1.5444

OA2G .32222 .58161 .997 -1.6717 2.3162

OA2E2 .46667 .34721 .819 -.7598 1.6931

MB1C -.10000 .36132 1.000 -1.3528 1.1528

SA2A -.02222 .50464 1.000 -1.7250 1.6805

OA1E 7.03333* 1.27279 .004 2.3174 11.7493

SA1A OA2E1 -.27778 .36772 .986 -1.5444 .9888

OA2G .04444 .52561 1.000 -1.8453 1.9341

OA2E2 .18889 .24197 .984 -.6288 1.0066

(3)

SA2A -.30000 .43892 .991 -1.8464 1.2464

OA1E 6.75556* 1.24820 .006 2.0472 11.4639

OA2G OA2E1 -.32222 .58161 .997 -2.3162 1.6717

SA1A -.04444 .52561 1.000 -1.9341 1.8453

OA2E2 .14444 .51147 1.000 -1.7313 2.0202

MB1C -.42222 .52116 .978 -2.3068 1.4624

SA2A -.34444 .62905 .997 -2.4653 1.7764

OA1E 6.71111* 1.32705 .005 1.9466 11.4756

OA2E2 OA2E1 -.46667 .34721 .819 -1.6931 .7598

SA1A -.18889 .24197 .984 -1.0066 .6288

OA2G -.14444 .51147 1.000 -2.0202 1.7313

MB1C -.56667 .23214 .246 -1.3483 .2150

SA2A -.48889 .42189 .895 -2.0111 1.0333

OA1E 6.56667* 1.24231 .008 1.8586 11.2748

MB1C OA2E1 .10000 .36132 1.000 -1.1528 1.3528

SA1A .37778 .26182 .772 -.5004 1.2560

OA2G .42222 .52116 .978 -1.4624 2.3068

OA2E2 .56667 .23214 .246 -.2150 1.3483

SA2A .07778 .43358 1.000 -1.4600 1.6156

OA1E 7.13333* 1.24633 .004 2.4251 11.8415

SA2A OA2E1 .02222 .50464 1.000 -1.6805 1.7250

SA1A .30000 .43892 .991 -1.2464 1.8464

OA2G .34444 .62905 .997 -1.7764 2.4653

OA2E2 .48889 .42189 .895 -1.0333 2.0111

MB1C -.07778 .43358 1.000 -1.6156 1.4600

OA1E 7.05556* 1.29516 .004 2.3244 11.7867

OA1E OA2E1 -7.03333* 1.27279 .004 -11.7493 -2.3174

SA1A -6.75556* 1.24820 .006 -11.4639 -2.0472

(4)

OA2E2 -6.56667* 1.24231 .008 -11.2748 -1.8586

MB1C -7.13333* 1.24633 .004 -11.8415 -2.4251

SA2A -7.05556* 1.29516 .004 -11.7867 -2.3244

*. The mean difference is significant at the 0.05 level.

b.

Data pH media kultur dengan senyawa hidrokarbon alifatik

NPar Tests

ISOLAT WAKTU pH

N 63 63 63

Std. Deviation 2.016 2.89047 .32165

Positive .125 .213 .173

Negative -.125 -.231 -.298

Asymp. Sig. (2-tailed) .277 .002 .000

Oneway

pH

(5)

ANOVA

pH

Between Groups .065 6 .011 .096 .996

Within Groups 6.349 56 .113

Total 6.414 62

Post Hoc Tests

Homogeneous Subsets

pH

Duncan

ISOLAT N

Subset for alpha = 0.05

1

OA2E1 9 6.7444

OA1E 9 6.7556

SA1A 9 6.7889

OA2E2 9 6.7889

SA2A 9 6.7889

OA2G 9 6.8222

MB1C 9 6.8444

Sig. .594

Means for groups in homogeneous

(6)

c.

Data TPC bakteri pada senyawa hidrokarbon monoaromatik

NPar Tests

ISOLAT WAKTU TPC

N 63 63 63

Std. Deviation 2.016 2.89047 1.65155

Positive .125 .213 .083

Negative -.125 -.231 -.121

Kolmogorov-Smirnov Z .993 1.834 .958

Asymp. Sig. (2-tailed) .277 .002 .317

Oneway

TPC

1.576 6 56 .171

ANOVA

TPC

Between Groups 20.722 6 3.454 1.303 .271

(7)

Post Hoc Tests

Homogeneous Subsets

TPC

Duncan

ISOLAT N

1 2

OA2E1 9 7.7333

MB1C 9 8.2778 8.2778

OA2E2 9 8.6444 8.6444

OA1E 9 8.8667 8.8667

SA1A 9 8.9889 8.9889

SA2A 9 9.1556 9.1556

OA2G 9 9.6444

Sig. .110 .125

Means for groups in homogeneous subsets are displayed.

d.

Data pH pada media kultur dengan senyawa hidrokarbon monoaromatik

NPar Tests

ISOLAT WAKTU pH

N 63 63 63

Std. Deviation 2.016 2.89047 .13879

Positive .125 .213 .219

Negative -.125 -.231 -.231

(8)

Asymp. Sig. (2-tailed) .277 .002 .002

Oneway

pH

.115 6 56 .994

ANOVA

pH

Between Groups .012 6 .002 .095 .997

Total 1.194 62

Post Hoc Tests

Homogeneous Subsets

pH

Duncan

ISOLAT N

1

OA2E2 9 6.8000

SA2A 9 6.8111

MB1C 9 6.8222

(9)

SA1A 9 6.8333

OA2G 9 6.8333

OA2E1 9 6.8444

Sig. .583

Means for groups in homogeneous subsets are displayed.

e.

Data TPC bakteri pada senyawa hidrokarbon poliaromatik

NPar Tests

ISOLAT WAKTU TPC

N 63 63 63

Std. Deviation 2.016 2.89047 2.73697

Positive .125 .213 .119

Negative -.125 -.231 -.152

Asymp. Sig. (2-tailed) .277 .002 .110

Oneway

TPC

(10)

ANOVA

TPC

Between Groups 21.251 6 3.542 .448 .844

Total 464.442 62

Homogeneous Subsets

TPC

Duncan

ISOLAT N

1

OA2E2 9 9.6444

OA2G 9 10.2222

SA1A 9 10.4000

OA1E 9 10.4444

OA2E1 9 10.8222

SA2A 9 11.3667

MB1C 9 11.3778

Sig. .268

(11)

f.

Data pH pada media kultur dengan senyawa hidrokarbon poliaromatik

NPar Tests

ISOLAT WAKTU pH

N 63 63 63

Std. Deviation 2.016 2.89047 .16458

Positive .125 .213 .158

Negative -.125 -.231 -.237

Asymp. Sig. (2-tailed) .277 .002 .002

Oneway

pH

(12)

ANOVA

pH

Between Groups .039 6 .007 .224 .967

Total 1.679 62

Post Hoc Tests

Homogeneous Subsets

pH

Duncan

ISOLAT N

1

OA2E1 9 6.7889

SA1A 9 6.8000

OA1E 9 6.8111

OA2G 9 6.8333

OA2E2 9 6.8333

MB1C 9 6.8444

SA2A 9 6.8667

Sig. .414

(13)

Lampiran 2

Hasil analisis GC

a.

Hasil analisis standar baku 1000 ppm (n-heksadekana, fenol, dan

(14)

(15)

(16)

(17)

(18)

(19)

d.

Kalibrasi n-heksadekana

(20)

f.

Kalibrasi fenantren

(21)

(22)

(23)

(24)

(25)

(26)

Lampiran 3

Perhitungan persentase degradasi

a.

N-heksadekana

Faktor kalibrasi



y= 0,043x + 520,2

Luas area hari ke-0



x= 4924, y= 0,043 . 4924 + 520,2

= 731,932 ppm

Luas area hari ke-7



x= 4659, y= 0,043 . 4659 + 520,2

= 720,537 ppm

b.

Fenol

Faktor kalibrasi



y= 0,843x + 210,3

Luas area hari ke-0



x= 630, y= 0,843 . 630 + 210,3

= 741,39 ppm

Luas area hari ke-7



x= 560, y= 0,843 . 560 + 210,3

= 682,38 ppm

c.

Fenantren

Faktor kalibrasi



y= 0,038x + 532

Luas area hari ke-0



x= 12224, y= 0,038 . 12224 + 532

= 996,512 ppm

Luas area hari ke-7



x= 8152, y= 0,038 . 8152 + 532

(27)

Lampiran 4

Hasil uji kit dan uji fisiologis

a.

SA2A

No.

Substrat

Warna

Hasil

Keterangan

1 Lysine

kuning

(-)

2 Ornithine

kuning

(-)

3 H2S

warna jerami

(-)

4 Glucose

hijau

(-)

5 Mannitol

biru - hijau

(-)

6 Xylose

kuning

(+)

7 ONPG

tidak berwarna

(-)

8 Indole

tidak berwarna

(-)

9 Urease

merah muda

(+)

10 VP

merah ceri

(+/-)

11 Citrate

kuning

(-)

12 TDA

kuning

(-)

13 Gelatin

tidak berwarna

(-)

14 Malonate

kuning

(-)

15 Inositol

biru - hijau

(-)

16 Sorbitol

biru - hijau

(-)

17 Rhamnose

hijau

(-)

18 Sucrose

biru - hijau

(-)

19 Lactose

biru - hijau

(-)

20 Arabinose

kuning

(+)

21 Adonitol

biru - hijau

(-)

22 Raffinose

biru - hijau

(-)

23 Salicin

biru - hijau

(-)

24 Arginine

kuning

(-)

25 Nitrat

kuning

(-)

26 Nitrat

(+Zn)

kuning

(+)

(28)

b.

OA2G

(29)

Tabel persamaan karakteristik

a.

Isolat bakteri SA2A pada uji amilase positif

Keterangan:

(T) spora terminal, (V) spora sentral atau subteminal, (X) spora oval,(Y) spora bulat.

(1) B. anthracis; (2) B. cereus, B. anthracoide;(3) B. mycoides;(4) B. thuringiensis;(5) B. firmus;(6) B. lentus;(7) B. megaterium;(9) B.subtilis;(10) B. licheniformis;(11) B. amyloliquefaciens;(12) B. coagulans;(14) B. alvei;(16)

B.circulans;(18) B. macerans;(19) B.polymyxa;(22) B. stearothermophilus;(24) B. Stearothermophilus

(30)

b.

Isolat bakteri OA2G pada uji amilase positif

Keterangan:

(T) spora terminal, (V) spora sentral atau subteminal, (X) spora oval,(Y) spora bulat.

(1) B. anthracis; (2) B. cereus, B. anthracoide;(3) B. mycoides;(4) B. thuringiensis;(5) B. firmus;(6) B. lentus;(7) B. megaterium;(9) B.subtilis;(10) B. licheniformis;(11) B. amyloliquefaciens;(12) B. coagulans;(14) B. alvei;(16)

B.circulans;(18) B. macerans;(19) B.polymyxa;(22) B. stearothermophilus;(24) B. stearothermophilus

(31)

Lampiran 5

Dokumentasi

a.

Alat dan bahan

Keterangan :

a= colony counter

d= spektrofotometer

g= shaker

b

=

autoclave

e= inkubator

h= kompor listrik

c= timbangan analitik

f= lemari es

Keterangan :

1= kapas 11= tabung reaksi 2= korek api 12= pipetor 3= sendok media 13= hand counter

4= cawan petri 14= botol kultur 5= tip 1000µL 15= gelas ukur 10 mL 6= tip 100 µL 16= bunsen

7= spatula besi 17= botol aquades 8= mikropipet 18= beaker glass

(32)

b.

Isolat bakteri

c.

Media kultur

n-heksadekana (hari ke-3 dan hari ke-7)

Senyawa murni hidrokarbon:

1= fenantren

2= n-heksadekana

3= fenol

(33)

Lanjut Gambar Media Kultur

Fenol (hari ke-3 dan hari ke-7)

Fenantren (hari ke-3 dan hari ke-7)

d.

Hasil TPC

Hari ke-3

Hari ke-7

Hari ke-3

Hari ke-7

(34)

Lanjutan Gambar Hasil TPC

e.

Hasil uji fisiologi bakteri menggunakan

microbact identification kit

OA2E1

OA2E2

OA2G

SA1A

(35)

f.

Hasil uji oksidase

g.

Hasil uji amilolitik

h.

Hasil uji proteolitik

i.

Hasil uji H

2

O

2

Keterangan:

Warna kertas berubah = positif

(36)

Tabel persamaan karakteristik

a.

Isolat bakteri SA2A pada uji amilase positif

(37)

Keterangan:

(T) spora terminal, (V) spora sentral atau subteminal, (X) spora oval,(Y) spora bulat.

(1)

B. anthracis; (2)

B. cereus, B. anthracoide;(3)

B. mycoides;(4)

B. thuringiensis;(5) B. firmus;(6)

B. lentus;(7)

B. megaterium;(9)

B.subtilis;(10)

B. licheniformis;(11)

B. amyloliquefaciens;(12)

B. coagulans;(14)

B. alvei;(16)

B.circulans;(18) B. macerans;(19) B.polymyxa;(22) B. stearothermophilus;(24) B. Stearothermophilus

16 ONPG - - - d - d + + + + d d d + + + d - 17 Citrate - - d d + - - + + + d d - - - d - - 18 Urease + - d d - - + d - d - - d - - - - - 19 Indole - - - + - - - - - 20

VP (Voges

Proskauer) d + + + + - - - + d + d + d d + d d

21

Reduksi

nitrate - + + + + + - d + + + d - d + + + d 22 Oksidase + d d d d - + - - - - - + - + - - -

23

Hidrolisis kasein

(Protein) + + + + + + - + + + + + + - - + - d

24

Hidrolisis tepung

(Amilum) + + + + + +

+ + + + + + + + + + + +

Koefisien sebanding

(38)

(39)

Keterangan:

(T) spora terminal, (V) spora sentral atau subteminal, (X) spora oval,(Y) spora bulat.

(1)

B. anthracis; (2)

B. cereus, B. anthracoide;(3)

B. mycoides;(4)

B. thuringiensis;(5) B. firmus;(6)

B. lentus;(7)

B. megaterium;(9)

B.subtilis;(10)

B. licheniformis;(11)

B. amyloliquefaciens;(12)

B. coagulans;(14)

B. alvei;(16)

B.circulans;(18) B. macerans;(19) B.polymyxa;(22) B. stearothermophilus;(24) B. Stearothermophilus

18 Urease - - d d - - + d - d - - d - - - - - 19 Indole - - - + - - - - - 20

VP (Voges

Proskauer) d + + + + - - - + d + d + d d + d d

21 Reduksi nitrate + + + + + + - d + + + d - d + + + d 22 Oksidase + d d d d - + - - - - - + - + - - - 23

Hidrolisis kasein

(Protein) + + + + + + - + + + + + + - - + - d

25

Hidrolisis tepung

(Amilum) + + + + + +

+ + + + + + + + + + + +

Koefisien sebanding