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Five Bacteremia Cases due to
Burkholderia gladioli
in ICU Sanglah General Hospital 2015
I Wayan Agus Gede Manik Saputra, Ni Nengah Dwi Fatmawati, Ni Nyoman Sri Budayanti, Ni Made Adi Tarini
Department of Clinical Microbiology, Faculty of Medicine and Health Sciences, Udayana University/Sanglah General Hospital Denpasar, Bali
Burkholderia gladioli is primarily a plant pathogen that can cause disease in severely immunocompromised patients. According to Bergey’s Manual of Systemic Bacteriology it is cathegorized into rRNA homology group II, not true pseudomonads and belongs to genus Burkholderia. The phytopathogen B.gladioli has been most commonly associated with diseases of the gladiolus plant and decaying onions. Bacteremia due to B.gladioli have never been identification of microorganism using Vitek2 system, but unfortunately this system could not detect accurately due to the high phenotypic similarity between this species and closely related species in the Burkholderia cepacia complex. After the isolates were identified as B. gladioli by Vitek2 system, the confirmation was performed by using PCR. The antimicrobial susceptibility pattern could not be gathered because the data was unavailable on Vitek2 system database. The antimicrobial susceptibility test (ASTs) were then conducted based on Kirby Bauer Disk Diffusion Methods. All AST results were analyzed using WHONET 5.6 version software. All five isolated B.gladioli were confirmed as the same organism by using two oligonucleotide primer pairs by PCR method. The origin of this bacterium, which caused an outbreak in ICU ward needs further investigations, and infection control investigators must be enrolled on this condition in order to prevent spreading this phytopathogen.
Keywords : Bacteremia, Burkholderia gladioli, ICU, Sanglah General Hospital PCR, Outbreak
References:
1. Mahon, C.R., Lehman, D.C., Manuselis, G. Textbook of Diagnostic Microbiology. 5th Edition. China : Elsevier, Saunders.2015. P.486
2. Graves M, Robin T, Chipman AM, Wong J, Khashe S, Janda JM. Four Additional Cases of Burkholderia gladioli infection with Microbiological Correlates and Review. Clinical Infectious Diseases. 1997;25:838-42
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