Lampiran 1. Hasil identifikasi sampel
Lampiran 2. Bagan kerja uji antiinflamasi
Keterangan :
Vo: volume kaki tikus sesaat setelah diinduksi karagenan, Vt: volume kaki tikus pada menit ke-t, K: kontrol Na CMC 0,5 %, P: perlakuan, P I: EEM dosis 200 mg/kg bb, P II: EEM dosis 300 mg/kg bb, P III: diberi EEM dosis 400 mg/kg bb, Pembanding: Na diklofenak 2,25 mg/kg bb
K P P II P III Pembanding
Hewan Uji (tikus)
Dipuasakan selama ± 18 jam, tetap diberi minum
Dibagi lima kelompok dan masing-masing diberi perlakuan secara peroral
30 menit kemudian masing-masing kaki tikus dari setiap kelompok diinduksi dengan 0,1 ml karagenan 1% secara subplantar
Diukur volume kaki tikus dengan mencelupkan kaki tikus sampai batas yang telah ditandai pada mata kaki ke dalam alat pletismometer
Vo
Diukur penambahan volume kaki tikus dengan cara yang sama pada pengukuran kaki tikus mula-mula
Lampiran 3. Contoh perhitungan dosis natrium diklofenak yang diberikan terhadap tikus secara per oral (p.o.)
- Tiap tablet natrium diklofenak mengandung 25 mg natrium diklofenak - Dosis maksimum untuk manusia dewasa = 25 – 50 mg
- Syarat volume maksimum larutan sediaan uji yang diberikan pada hewan uji tikus (200 g) secara peroral (p.o.) adalah 5,0 ml (Lampiran 5) - Konversi dosis manusia (70 kg) ke dosis hewan uji tikus dikali 0,018
(Lampiran 6 )
Pemberian larutan natrium diklofenak
a. Dosis natrium diklofenak yang diberikan (dalam mg/kg bb) = ( 25 mg- 50 mg)x 0,018
= 0,45 mg - 0,9 mg
Maka dosis natrium diklofenak yang digunakan adalah 0,45 mg untuk tikus 200 g, sehingga dosis dalam mg/kg bb adalah:
kg 1
x g 200
g 45 ,
0 m =
x x1000g g
200 mg 0,45 =
x=2,25mg
b. Jumlah dan volume suspensi natrium diklofenak yang diberikan pada tikus
Satu tablet natrium diklofenak mengandung 25 mg natrium diklofenak yang dilarutkan dalam10 ml suspensi Na CMC 0,5 %. - Maka volume larutan yang diberikan:
ml x
x 10
mg 25
mg 0,45 =
Lampiran 4. Perhitungan dosis pemberian EEM terhadap tikus secara peroral (p.o.)
Konsentrasi EEM = 5% = 5 g/100 ml = 500 mg/10 ml Berat badan tikus = 200 g
a. EEM yang diberikan untuk dosis 200 mg/kg bb Dosis = 200 mg/kg bb
b. EEM yang diberikan untuk dosis 300 mg/kg bb Dosis = 300 mg/kg bb
Lampiran 5. Tabel volume maksimum larutan sediaan uji yang dapat diberikan pada hewan uji (Mulyono, 1984)
Jenis hewan uji Volume maksimum (ml) sesuai jalur pemberian
i.v. i.m. i.p. s.c. p.o.
Mencit (20-30 g) 0,5 0,05 1,0 0,5-1,0 1,0
Tikus (200 g) 1,0 0,1 2-5 2-5 5,0
Hamster (50 g) - 0,1 1-2 2,5 2,5
Marmot (300 g) - 0,25 2-5 5,0 10,0
Kelinci (2,5 mg/kg) 5-10 0,5 10-20 5-10 20,0
Kucing (3 mg/kg) 5-10 1,0 10-20 5-10 50,0
Lampiran 6. Tabel konversi dosis antara jenis hewan dengan manusia (Laurance dan Bacharach, 1964)
Mencit 20 g
Tikus 200 g
Marmot 400 g
Kelinc 1,5 mg/kg
Kera 4 mg/kg
Anjing 12 mg/kg
Manusia 70 mg/kg Mencit
20 g 1,0 7,0 12,25 27,8 64,1 124,3 387,9
Tikus
200 g 0,14 1,0 1,74 3,0 9,2 17,8 56,0
Marmot
400 g 0,008 0,57 1,0 2,25 5,2 10,2 31,5
Kelinci 1,5 mg/kg
0,04 0,25 0,44 1,0 2,4 4,5 14,2
Kera
4 mg/kg 0,016 0,11 0,19 0,42 1,0 1,9 6,1
Anjing 12 mg/kg
0,008 0,06 0,10 0,22 0,52 1,0 3,1
Manusia 70 mg/kg
Lampiran 7. Contoh perhitungan persen radang, persen inhibisi radang dan AUC persentase radang
Misal: pemberian ekstrak etanol majakani dosis 200 mg/kg BB untuk menit ke-60
dimana :Vt = volume radang setelah waktu t Vo = volume awal kaki tikus 2. Persen inhibisi radang
%
dimana : a = persen radang rata-rata kelompok kontrol
Lampiran 7. (Lanjutan)
3. AUC persentase inhibisi radang
[ ]
(
)
tn = waktu pengamatan dari persentase radang ke n tn-1 = waktu pengamatan sebelumnya yang
berhubungan dengan persentae radang ke n-1
%Rn-1 = persen radang ke n-1 %Rn = persen radang ke n
Misalnya contoh perhitungan AUC persentase radang Na CMC pada tikus pertama (Lampiran 8):
Lampiran 8. Data hasil pengukuran penambahan volume kaki tikus (ml), persentase radang dan persentase inhibisi radang pada t (menit) setelah pemberian bahan uji dalam bentuk suspensi dan penyuntikan karagenan
I. Kontrol CMC Na 0.5%
15 30 45 60 75 90
Vo Vt %R Vt %R Vt %R Vt %R Vt %R Vt %R
I 0.01 0.011 10.00 0.011 10.00 0.012 20.00 0.012 20.00 0.013 30.00 0.014 40.00
II 0.014 0.015 7.14 0.016 14.28 0.020 42.80 0.022 57.14 0.024 71.43 0.028 100.00
III 0.015 0.015 0.00 0.016 6.67 0.018 20.00 0.020 33.33 0.022 46.67 0.022 46.67
IV 0.014 0.015 7.14 0.015 7.14 0.016 14.28 0.019 35.70 0.020 42.86 0.022 57.14
V 0.012 0.013 8.33 0.013 8.33 0.015 25.00 0.017 41.67 0.019 58.33 0.020 66.67
VI 0.013 0.014 7.69 0.014 7.69 0.016 23.08 0.017 30.77 0.018 38.46 0.020 53.84 Rerata %R 6.72 9.02 24.19 36.44 47.96 60.72
%IR 0.00 0.00 0.00 0.00 0.00 0.00
105 120 135 150 165 180 195
Vt %R Vt %R Vt %R Vt %R Vt %R Vt %R Vt %R 0.015 50.00 0.016 60.00 0.017 70.00 0.018 80.00 0.018 80.00 0.018 80.00 0.017 70.00 0.028 100.00 0.028 100.00 0.028 100.00 0.029 107.00 0.029 107.00 0.028 100.00 0.028 100.00 0.024 60.00 0.026 73.33 0.026 73.33 0.028 86.67 0.028 86.67 0.028 86.67 0.028 86.67 0.024 71.43 0.027 92.85 0.027 92.85 0.027 92.85 0.027 92.85 0.027 92.85 0.026 85.71 0.021 75.00 0.022 83.33 0.022 83.33 0.022 83.33 0.022 83.33 0.023 91.67 0.022 83.33 0.022 69.23 0.023 76.92 0.024 84.62 0.24 84.62 0.024 84.62 0.024 84.62 0.024 84.62
70.94 81.07 84.02 89.08 89.08 89.30 85.06 0.00 0.00 0.00 0.00 0.00 0.00 0.00
210 225 240 255 270 285 300
Vt %R Vt %R Vt %R Vt %R Vt %R Vt %R Vt %R 0.017 70.00 0.017 70.00 0.016 60.00 0.016 60.00 0.015 50.00 0.015 50.00 0.015 50.00 0.027 92.85 0.027 92.85 0.027 92.85 0.026 85.71 0.026 85.71 0.026 85.71 0.026 85.71 0.028 86.67 0.027 80.00 0.026 73.33 0.026 73.33 0.025 66.67 0.025 66.67 0.024 60.00 0.026 85.71 0.024 71.43 0.024 71.43 0.024 71.43 0.024 71.43 0.023 64.30 0.023 64.30 0.022 83.33 0.021 75.00 0.021 75.00 0.020 66.67 0.020 66.67 0.020 66.67 0.020 66.67 0.023 76.92 0.023 76.92 0.022 69.23 0.022 69.23 0.022 69.23 0.022 69.23 0.021 61.54
82.58 77.70 73.64 71.06 68.29 67.10 64.70 0.00 0.00 0.00 0.00 0.00 0.00 0.00
315 330 345 360
Vt %R Vt %R Vt %R Vt %R 0.014 40.00 0.014 40.00 0.014 40.00 0.014 40.00 0.026 85.71 0.026 85.71 0.025 78.60 0.025 78.60 0.023 53.33 0.023 53.33 0.023 53.33 0.023 53.33 0.022 57.14 0.022 57.14 0.022 57.14 0.022 57.14 0.019 58.33 0.019 58.33 0.019 58.33 0.019 58.33 0.021 61.54 0.021 61.54 0.020 53.85 0.020 53.85
Lampiran 8. (Lanjutan)
II. Pembanding Na diklofenak dosis 2,25 mg/kg bb
t = 15 t = 30 t = 45 t = 60 t = 75 t = 90
Vo Vt %R Vt %R Vt %R Vt %R Vt %R Vt %R
I 0.012 0.012 0.00 0.012 0.00 0.012 0.00 0.014 16.67 0.016 33.33 0.018 50.00
II 0.012 0.013 8.33 0.013 8.33 0.013 8.33 0.014 16.67 0.015 25.00 0.016 33.33
III 0.013 0.014 7.69 0.014 7.69 0.014 7.69 0.015 15.38 0.016 23.08 0.016 23.08
IV 0.013 0.013 0.00 0.013 0.00 0.014 7.69 0.015 15.13 0.016 15.38 0.017 26.67
V 0.015 0.015 0.00 0.015 0.00 0.016 6.67 0.016 6.67 0.018 20.00 0.019 26.67
VI 0.016 0.016 0.00 0.016 0.00 0.016 0.00 0.018 12.50 0.019 18.75 0.020 25.00
Rerata %R 2.67 2.67 5.06 13.84 22.59 30.79
%IR 60.25 70.39 79.07 62.02 52.90 49.29
t = 105 t = 120 t = 135 t = 150 t = 165 t = 180 t = 195
Vt %R Vt %R Vt %R Vt %R Vt %R Vt %R Vt %R
0.018 50.00 0.019 58.33 0.02 66.67 0.021 75.00 0.022 83.33 0.022 83.33 0.022 83.33
0.017 41.67 0.017 41.67 0.017 41.67 0.018 50.00 0.019 58.33 0.02 66.67 0.02 66.67
0.016 23.08 0.016 23.08 0.017 30.77 0.017 30.77 0.018 38.46 0.018 38.46 0.018 38.46
0.017 26.67 0.019 46.15 0.021 61.54 0.021 61.54 0.022 69.23 0.022 69.23 0.022 69.23
0.02 33.33 0.021 40.00 0.022 46.67 0.022 46.67 0.023 53.33 0.023 53.33 0.023 53.33
0.021 31.25 0.021 31.25 0.023 43.75 0.024 50.00 0.024 50.00 0.026 62.50 0.026 62.50
34.33 40.08 48.51 52.33 58.78 62.25 62.25
Lampiran 8. (Lanjutan)
t = 210 t = 225 t = 240 t = 255 t = 270 t = 285 t = 300
Vt %R Vt %R Vt %R Vt %R Vt %R Vt %R Vt %R
0.021 75.00 0.021 75.00 0.02 66.67 0.019 58.33 0.019 58.33 0.018 50.00 0.018 50.00
0.02 66.67 0.019 58.33 0.019 50.00 0.017 41.67 0.017 41.67 0.017 41.67 0.017 41.67
0.018 38.46 0.018 38.46 0.018 38.46 0.018 38.46 0.017 30.77 0.017 30.77 0.017 30.77
0.022 69.23 0.021 61.54 0.021 61.54 0.020 53.85 0.021 61.54 0.021 61.54 0.021 61.54
0.024 60.00 0.024 60.00 0.022 46.67 0.021 40.00 0.021 40.00 0.021 40.00 0.021 40.00
0.026 62.50 0.025 56.25 0.023 43.75 0.022 37.50 0.022 37.50 0.021 31.25 0.021 31.25
61.98 58.26 51.18 44.97 44.97 42.54 42.54
24.95 25.02 30.50 36.72 34.15 36.60 34.26
t = 315 t = 330 t = 345 t = 360
Vt %R Vt %R Vt %R Vt %R
0.018 50.00 0.018 50.00 0.017 41.67 0.017 41.67
0.016 33.33 0.016 33.33 0.016 33.33 0.016 33.33
0.017 30.77 0.017 30.77 0.016 23.08 0.016 23.08
0.020 53.84 0.020 53.84 0.020 53.84 0.020 53.84
0.021 40.00 0.021 40.00 0.021 40.00 0.021 40.00
0.021 31.25 0.020 25.00 0.020 25.00 0.020 25.00
39.87 38.82 36.15 36.15
Lampiran 8. (Lanjutan)
III. EEM dosis 200 mg/kg bb
t = 15 t = 30 t = 45 t = 60 t = 75 t = 90
Vo Vt %R Vt %R Vt %R Vt %R Vt %R Vt %R
I 0.012 0.013 8.33 0.014 16.66 0.015 25.00 0.015 25.00 0.017 41.66 0.019 58.33
II 0.014 0.015 7.14 0.015 7.14 0.017 21.42 0.019 35.71 0.019 35.71 0.020 42.84
III 0.017 0.018 5.88 0.018 5.88 0.019 11.76 0.020 17.65 0.021 23.53 0.023 35.29
IV 0.015 0.016 6.67 0.016 6.67 0.016 6.67 0.018 20.00 0.019 26.67 0.021 40.00
V 0.014 0.014 0.00 0.015 7.14 0.016 14.28 0.017 21.42 0.018 28.57 0.020 42.86
VI 0.013 0.014 7.69 0.014 7.69 0.016 23.07 0.018 38.46 0.019 46.15 0.021 61.53
Rerata %R 5.95 8.53 17.03 26.37 33.72 46.81
%IR 11.39 5.43 29.60 27.62 29.70 22.91
t = 105 t = 120 t = 135 t = 150 t = 165 t = 180 t = 195
Vt %R Vt %R Vt %R Vt %R Vt %R Vt %R Vt %R
0.021 75.00 0.022 83.33 0.022 83.33 0.023 91.67 0.023 91.67 0.022 83.33 0.022 83.33
0.021 50.00 0.022 57.14 0.023 64.28 0.023 64.24 0.024 71.43 0.024 71.43 0.023 64.24
0.025 47.06 0.027 58.82 0.028 64.70 0.028 64.70 0.028 64.70 0.027 58.82 0.026 52.94
0.022 46.66 0.024 60.00 0.025 66.67 0.025 66.67 0.025 66.67 0.025 66.67 0.024 60.00
0.022 57.14 0.024 71.43 0.025 78.57 0.026 85.71 0.026 85.71 0.026 85.71 0.025 78.57
0.021 61.53 0.022 69.23 0.022 69.23 0.023 76.92 0.023 76.92 0.022 69.23 0.021 61.53
56.23 66.66 71.13 74.99 76.18 72.53 66.77
Lampiran 8. (Lanjutan)
t = 210 t = 225 t = 240 t = 255 t = 270 t = 285 t = 300
Vt %R Vt %R Vt %R Vt %R Vt %R Vt %R Vt %R
0.021 75.00 0.021 75.00 0.021 75.00 0.020 66.67 0.020 66.67 0.020 66.67 0.021 75.00
0.023 64.24 0.022 57.14 0.022 57.14 0.022 57.14 0.022 57.14 0.022 57.14 0.023 64.24
0.026 52.94 0.025 47.06 0.025 47.06 0.025 47.06 0.024 41.78 0.025 47.06 0.025 47.17
0.024 60.00 0.023 53.33 0.023 53.33 0.023 53.33 0.023 53.33 0.023 53.33 0.022 46.66
0.024 71.43 0.024 71.43 0.023 71.43 0.023 71.43 0.023 71.43 0.023 71.43 0.023 71.43
0.021 61.53 0.021 61.53 0.020 53.84 0.020 53.84 0.020 53.84 0.020 53.84 0.020 53.84
64.19 60.92 59.63 58.25 57.37 58.25 59.72
22.27 21.69 19.02 18.04 14.70 13.19 79.84
t = 315 t = 330 t = 345 t = 360
Vt %R Vt %R Vt %R Vt %R
0.020 66.67 0.020 66.67 0.019 58.33 0.019 58.33
0.022 57.14 0.022 57.14 0.022 57.14 0.022 57.14
0.025 47.17 0.024 41.78 0.024 41.78 0.024 41.78
0.022 46.66 0.022 46.66 0.022 46.66 0.022 46.66
0.023 71.43 0.022 57.14 0.022 57.14 0.022 57.14
0.020 53.84 0.020 53.84 0.020 53.84 0.020 53.84
57.15 53.87 52.48 52.48
Lampiran 8. (Lanjutan)
IV. EEM dosis 300 mg/kg bb
t = 15 t = 30 t = 45 t = 60 t = 75 t = 90
Vo Vt %R Vt %R Vt %R Vt %R Vt %R Vt %R
I 0.013 0.014 7.69 0.014 7.69 0.014 7.69 0.014 7.69 0.015 15.38 0.015 15.38
II 0.014 0.015 7.14 0.015 7.14 0.015 7.14 0.017 21.43 0.019 35.71 0.020 42.86
III 0.015 0.016 6.67 0.017 13.33 0.017 13.33 0.017 13.33 0.018 20.00 0.019 26.67
IV 0.016 0.016 0.00 0.016 0.00 0.018 12.50 0.018 12.50 0.019 18.75 0.020 25.00
V 0.014 0.015 7.14 0.015 7.14 0.016 14.28 0.016 14.28 0.017 21.43 0.017 21.43
VI 0.015 0.015 0.00 0.016 6.67 0.016 6.67 0.017 13.33 0.019 26.66 0.020 33.33
Rerata %R 4.77 7.00 10.27 13.76 22.99 27.45
%IR 28.93 22.43 57.56 62.23 52.07 133.40
t = 105 t = 120 t = 135 t = 150 t = 165 t = 180 t = 195
Vt %R Vt %R Vt %R Vt %R Vt %R Vt %R Vt %R
0.016 18.75 0.016 18.75 0.017 30.76 0.019 46.15 0.019 46.15 0.019 46.15 0.019 46.15
0.021 50.00 0.022 57.14 0.025 78.57 0.026 85.71 0.027 92.85 0.027 92.85 0.027 92.85
0.020 33.33 0.021 40.00 0.021 40.00 0.021 40.00 0.021 40.00 0.021 40.00 0.021 40.00
0.021 31.25 0.023 43.75 0.025 56.25 0.025 56.25 0.025 56.25 0.026 62.50 0.026 62.50
0.018 28.57 0.019 35.71 0.020 42.86 0.022 57.14 0.023 64.28 0.023 64.28 0.023 64.28
0.021 40.00 0.021 40.00 0.022 46.67 0.023 53.33 0.023 53.33 0.024 60.00 0.024 60.00
33.65 39.23 49.19 56.43 58.81 60.96 60.96
Lampiran 8. (Lanjutan)
t = 210 t = 225 t = 240 t = 255 t = 270 t = 285 t = 300
Vt %R Vt %R Vt %R Vt %R Vt %R Vt %R Vt %R
0.019 46.15 0.018 38.46 0.018 38.46 0.018 38.46 0.018 38.46 0.018 38.46 0.018 38.46
0.027 92.85 0.027 92.85 0.025 78.57 0.024 71.43 0.024 71.43 0.023 64.30 0.022 57.14
0.020 33.33 0.020 33.33 0.019 26.67 0.019 26.67 0.019 26.67 0.019 26.67 0.019 26.67
0.026 62.50 0.026 62.50 0.025 50.00 0.024 50.00 0.024 50.00 0.024 50.00 0.024 50.00
0.023 64.28 0.023 64.28 0.022 57.14 0.021 50.00 0.021 50.00 0.021 50.00 0.021 50.00
0.024 60.00 0.024 60.00 0.023 53.33 0.022 46.67 0.022 46.67 0.021 40.00 0.021 40.00
59.85 58.57 50.70 47.21 47.21 44.91 43.71
27.52 24.62 31.16 33.57 30.87 33.07 32.45
t = 315 t = 330 t = 345 t = 360
Vt %R Vt %R Vt %R Vt %R
0.017 30.77 0.017 30.77 0.016 23.07 0.016 23.07
0.022 57.14 0.022 57.14 0.022 57.14 0.021 50.00
0.018 20.00 0.018 20.00 0.018 20.00 0.018 20.00
0.024 50.00 0.024 50.00 0.024 50.00 0.024 50.00
0.019 35.71 0.019 35.71 0.019 35.71 0.019 35.71
0.020 33.33 0.020 33.33 0.020 33.33 0.020 33.33
37.83 37.83 36.54 35.35
Lampiran 8. (Lampiran)
V. EEM dosis 400 mg/kg bb
t = 15 t = 30 t = 45 t = 60 t = 75 t = 90
Vo Vt %R Vt %R Vt %R Vt %R Vt %R Vt %R
I 0.018 0.02 11.11 0.02 11.11 0.02 11.11 0.02 11.11 0.021 16.67 0.022 22.22
II 0.014 0.014 0.00 0.014 0.00 0.014 0.00 0.015 7.14 0.016 12.50 0.018 28.57
III 0.018 0.018 0.00 0.018 0.00 0.018 0.00 0.019 5.56 0.020 11.11 0.020 11.11
IV 0.016 0.016 0.00 0.016 0.00 0.016 0.00 0.018 12.50 0.020 25.00 0.020 25.00
V 0.015 0.016 6.67 0.016 6.67 0.016 6.67 0.017 13.33 0.018 20.00 0.019 26.67
VI 0.016 0.016 0.00 0.016 0.00 0.018 12.50 0.018 12.50 0.019 18.75 0.020 25.00
Rerata %R 2.96 2.96 5.05 10.36 17.34 23.10
%IR 55.88 67.14 79.14 71.58 63.85 61.96
t = 105 t = 120 t = 135 t = 150 t = 165 t = 180 t = 195
Vt %R Vt %R Vt %R Vt %R Vt %R Vt %R Vt %R
0.023 27.78 0.023 27.78 0.022 22.22 0.022 22.22 0.020 11.11 0.020 11.11 0.020 11.11
0.019 35.71 0.02 42.85 0.022 57.14 0.022 57.14 0.022 57.14 0.023 64.28 0.023 64.28
0.022 22.22 0.024 33.33 0.024 33.33 0.025 38.89 0.025 38.89 0.024 33.33 0.024 33.33
0.022 37.50 0.023 43.75 0.025 56.25 0.026 62.50 0.027 68.75 0.027 68.75 0.026 62.50
0.021 40.00 0.022 46.67 0.023 53.33 0.024 60.00 0.024 60.00 0.024 60.00 0.023 53.33
0.021 31.25 0.022 37.50 0.023 43.75 0.024 50.00 0.024 50.00 0.023 43.75 0.023 43.75
32.41 38.65 44.34 48.46 47.65 46.87 44.72
Lampiran 8. (Lanjutan)
t = 210 t = 225 t = 240 t = 255 t = 270 t = 285 t = 300
Vt %R Vt %R Vt %R Vt %R Vt %R Vt %R Vt %R
0.019 5.56 0.019 5.56 0.019 5.56 0.019 5.56 0.019 5.56 0.019 5.56 0.019 5.56
0.023 64.28 0.023 64.28 0.022 57.14 0.021 50.00 0.021 50.00 0.020 42.85 0.020 42.85
0.024 33.33 0.024 33.33 0.024 33.33 0.024 33.33 0.024 33.33 0.024 33.33 0.024 33.33
0.026 62.50 0.025 56.25 0.024 50.00 0.024 50.00 0.024 50.00 0.024 50.00 0.024 50.00
0.023 53.33 0.023 53.33 0.023 53.33 0.022 46.67 0.022 46.67 0.022 46.67 0.022 46.67
0.023 43.75 0.022 37.50 0.022 37.50 0.021 31.25 0.021 31.25 0.021 31.25 0.021 31.25
43.79 41.71 39.48 36.14 36.14 34.94 34.94
46.97 46.32 46.39 49.15 47.08 47.92 47.92
t = 315 t = 330 t = 345 t = 360
Vt %R Vt %R Vt %R Vt %R
0.019 5.56 0.019 5.56 0.019 5.56 0.019 5.56
0.020 42.85 0.020 42.85 0.020 42.85 0.019 35.71
0.023 27.78 0.023 27.78 0.023 27.78 0.022 11.11
0.023 43.75 0.023 43.75 0.022 37.50 0.022 37.50
0.021 40.00 0.021 40.00 0.021 40.00 0.020 33.33
0.020 25.00 0.020 25.00 0.020 25.00 0.020 25.00
30.82 30.82 29.78 24.70
Lampiran 9. Data AUC persentase radang
I. Kontrol Na CMC 0,5 %
Luas Area Dibawah Kurva
Jumlah
uji Perlakuan
Luas Area Dibawah Kurva
Lampiran 9. Lanjutan II. EEM dosis 200 mg/kg bb
Luas Area Dibawah Kurva
Jumlah
uji Perlakuan
Luas Area Dibawah Kurva
Lampiran 9. Lanjutan III. EEM dosis 300 mg/kg bb
Luas Area Dibawah Kurva
Jumlah
uji Perlakuan
Luas Area Dibawah Kurva
Lampiran 9. Lanjutan IV. EEM dosis 400 mg/kg bb
Luas Area Dibawah Kurva
Jumlah
uji Perlakuan
Luas Area Dibawah Kurva
Lampiran 9. Lanjutan
IV. Pembanding Na diklofenak dosis 2,25 mg/kg bb
Luas Area Dibawah Kurva
Jumlah
uji Perlakuan
Luas Area Dibawah Kurva
Lampiran 10. Data AUC persentase radang
I. Kontrol Na CMC 0,5 %
Luas Area Dibawah Kurva
Jumlah
uji Perlakuan
Luas Area Dibawah Kurva
Lampiran 9. Lanjutan II. EEM dosis 200 mg/kg bb
Luas Area Dibawah Kurva
Jumlah
uji Perlakuan
Luas Area Dibawah Kurva
Lampiran 9. Lanjutan III. EEM dosis 300 mg/kg bb
Luas Area Dibawah Kurva
Jumlah
uji Perlakuan
Luas Area Dibawah Kurva
Lampiran 9. Lanjutan IV. EEM dosis 400 mg/kg bb
Luas Area Dibawah Kurva
Jumlah
uji Perlakuan
Luas Area Dibawah Kurva
Lampiran 9. Lanjutan
IV. Pembanding Na diklofenak dosis 2,25 mg/kg bb
Luas Area Dibawah Kurva
Jumlah
uji Perlakuan
Luas Area Dibawah Kurva
Lampiran 11. Hasil analisis deskriptif persen radang dengan SPSS
Descriptives
N Mean
Std.
Deviation Std. Error
95% Confidence Interval for Mean
Minimum Maximum Lower Bound Upper Bound
Menit 15 CMC Na 6 6.7167 3.45840 1.41188 3.0873 10.3460 .00 10.00
EEM 200 mg/Kg BB 6 5.9517 3.03427 1.23873 2.7674 9.1359 .00 8.33
EEM 300 mg/Kg BB 6 4.7733 3.71149 1.51521 .8784 8.6683 .00 7.69
EEM 400 mg/Kg BB 6 2.9633 4.80069 1.95987 -2.0747 8.0013 .00 11.11
Na Diklofenak 2.25% 6 2.6700 4.14129 1.69068 -1.6760 7.0160 .00 8.33
Total 30 4.6150 3.94853 .72090 3.1406 6.0894 .00 11.11
Menit 30 CMC Na 6 9.0183 2.82595 1.15369 6.0527 11.9840 6.67 14.28
EEM 200 mg/Kg BB 6 8.5300 4.02862 1.64468 4.3022 12.7578 5.88 16.66
EEM 300 mg/Kg BB 6 6.9950 4.23540 1.72909 2.5502 11.4398 .00 13.33
EEM 400 mg/Kg BB 6 2.9633 4.80069 1.95987 -2.0747 8.0013 .00 11.11
Na Diklofenak 2.25% 6 2.6700 4.14129 1.69068 -1.6760 7.0160 .00 8.33
Total 30 6.0353 4.67048 .85271 4.2913 7.7793 .00 16.66
Menit 45 CMC Na 6 24.1933 9.81367 4.00641 13.8945 34.4921 14.28 42.80
EEM 200 mg/Kg BB 6 17.0333 7.23795 2.95488 9.4376 24.6291 6.67 25.00
EEM 300 mg/Kg BB 6 10.2683 3.45919 1.41221 6.6381 13.8985 6.67 14.28
EEM 400 mg/Kg BB 6 5.0467 5.85419 2.38996 -1.0969 11.1903 .00 12.50
Na Diklofenak 2.25% 6 5.0633 3.95793 1.61582 .9097 9.2169 .00 8.33
Total 30 12.3210 9.63249 1.75864 8.7242 15.9178 .00 42.80
Menit 60 CMC Na 6 36.4350 12.39746 5.06124 23.4247 49.4453 20.00 57.14
EEM 200 mg/Kg BB 6 26.3733 8.67639 3.54212 17.2680 35.4786 17.65 38.46
EEM 300 mg/Kg BB 6 13.7600 4.42497 1.80648 9.1163 18.4037 7.69 21.43
EEM 400 mg/Kg BB 6 10.3567 3.22338 1.31594 6.9739 13.7394 5.56 13.33
Na Diklofenak 2.25% 6 13.8367 3.82774 1.56267 9.8197 17.8536 6.67 16.67
Total 30 20.1523 12.10753 2.21052 15.6313 24.6734 5.56 57.14
Menit 75 CMC Na 6 47.9583 14.82473 6.05217 32.4007 63.5159 30.00 71.43
EEM 200 mg/Kg BB 6 33.7150 8.96180 3.65864 24.3102 43.1198 23.53 46.15
EEM 400 mg/Kg BB 6 17.3383 5.10738 2.08508 11.9785 22.6982 11.11 25.00
Na Diklofenak 2.25% 6 22.5900 6.24233 2.54842 16.0391 29.1409 15.38 33.33
Total 30 28.9180 13.96703 2.55002 23.7026 34.1334 11.11 71.43
Menit 90 CMC Na 6 60.720 21.2847 8.6894 38.383 83.057 40.0 100.0
EEM 200 mg/Kg BB 6 46.808 10.5815 4.3199 35.704 57.913 35.3 61.5
EEM 300 mg/Kg BB 6 27.445 9.5977 3.9182 17.373 37.517 15.4 42.9
EEM 400 mg/Kg BB 6 23.095 6.2357 2.5457 16.551 29.639 11.1 28.6
Na Diklofenak 2.25% 6 30.792 10.0239 4.0922 20.272 41.311 23.1 50.0
Total 30 37.772 18.4360 3.3659 30.888 44.656 11.1 100.0
Menit 105 CMC Na 6 70.9433 16.86593 6.88549 53.2436 88.6430 50.00 100.00
EEM 200 mg/Kg BB 6 56.2317 10.91529 4.45615 44.7768 67.6866 46.66 75.00
EEM 300 mg/Kg BB 6 33.6500 10.59551 4.32560 22.5307 44.7693 18.75 50.00
EEM 400 mg/Kg BB 6 32.4100 6.64868 2.71431 25.4326 39.3874 22.22 40.00
Na Diklofenak 2.25% 6 34.3333 9.95137 4.06263 23.8900 44.7767 23.08 50.00
Total 30 45.5137 19.01858 3.47230 38.4120 52.6153 18.75 100.00
Menit 120 CMC Na 6 81.0717 14.30591 5.84036 66.0585 96.0848 60.00 100.00
EEM 200 mg/Kg BB 6 66.6583 10.03691 4.09755 56.1252 77.1914 57.14 83.33
EEM 300 mg/Kg BB 6 39.2250 12.44351 5.08004 26.1663 52.2837 18.75 57.14
EEM 400 mg/Kg BB 6 38.6467 7.15195 2.91977 31.1412 46.1522 27.78 46.67
Na Diklofenak 2.25% 6 40.0800 12.16068 4.96458 27.3181 52.8419 23.08 58.33
Total 30 53.1363 20.77746 3.79343 45.3779 60.8948 18.75 100.00
Menit 135 CMC Na 6 84.0217 11.35783 4.63682 72.1024 95.9410 70.00 100.00
EEM 200 mg/Kg BB 6 71.1300 7.94937 3.24532 62.7876 79.4724 64.28 83.33
EEM 300 mg/Kg BB 6 49.1850 16.63471 6.79109 31.7279 66.6421 30.76 78.57
EEM 400 mg/Kg BB 6 44.3367 14.12353 5.76591 29.5149 59.1584 22.22 57.14
Na Diklofenak 2.25% 6 48.5117 13.31993 5.43784 34.5333 62.4901 30.77 66.67
Total 30 59.4370 19.81876 3.61839 52.0366 66.8374 22.22 100.00
Menit 150 CMC Na 6 89.0783 9.76266 3.98559 78.8330 99.3236 80.00 107.00
EEM 200 mg/Kg BB 6 74.9917 11.71901 4.78427 62.6933 87.2900 64.28 91.67
EEM 300 mg/Kg BB 6 56.4300 15.76761 6.43710 39.8829 72.9771 40.00 85.71
EEM 400 mg/Kg BB 6 48.4583 15.41512 6.29320 32.2812 64.6355 22.22 62.50
Menit 165 CMC Na 6 89.0783 9.76266 3.98559 78.8330 99.3236 80.00 107.00
EEM 200 mg/Kg BB 6 76.1833 10.73407 4.38217 64.9186 87.4481 64.70 91.67
EEM 300 mg/Kg BB 6 58.8100 18.65110 7.61428 39.2369 78.3831 40.00 92.85
EEM 400 mg/Kg BB 6 47.6483 20.51938 8.37700 26.1146 69.1821 11.11 68.75
Na Diklofenak 2.25% 6 58.7800 15.69586 6.40781 42.3082 75.2518 38.46 83.33
Total 30 66.1000 20.83614 3.80414 58.3197 73.8803 11.11 107.00
Menit 180 CMC Na 6 89.3017 7.04299 2.87529 81.9105 96.6928 80.00 100.00
EEM 200 mg/Kg BB 6 72.5317 10.24457 4.18233 61.7806 83.2827 58.82 85.71
EEM 300 mg/Kg BB 6 60.9633 18.38448 7.50543 41.6700 80.2567 40.00 92.85
EEM 400 mg/Kg BB 6 46.8700 22.02763 8.99274 23.7534 69.9866 11.11 68.75
Na Diklofenak 2.25% 6 62.2467 15.23234 6.21858 46.2613 78.2320 38.42 83.33
Total 30 66.3827 20.33410 3.71248 58.7898 73.9755 11.11 100.00
Menit 195 CMC Na 6 85.0550 9.55205 3.89961 75.0307 95.0793 70.00 100.00
EEM 200 mg/Kg BB 6 66.7683 11.69986 4.77645 54.4901 79.0466 52.94 83.33
EEM 300 mg/Kg BB 6 60.9633 18.38448 7.50543 41.6700 80.2567 40.00 92.85
EEM 400 mg/Kg BB 6 44.7167 20.16511 8.23237 23.5547 65.8787 11.11 64.28
Na Diklofenak 2.25% 6 62.2533 15.21983 6.21347 46.2811 78.2256 38.46 83.33
Total 30 63.9513 19.50461 3.56104 56.6682 71.2345 11.11 100.00
Menit 210 CMC Na 6 82.5800 8.03558 3.28051 74.1472 91.0128 70.00 92.85
EEM 200 mg/Kg BB 6 64.1900 8.00588 3.26839 55.7883 72.5917 52.94 75.00
EEM 300 mg/Kg BB 6 59.8517 20.03332 8.17857 38.8280 80.8754 33.33 92.85
EEM 400 mg/Kg BB 6 43.7917 22.05385 9.00345 20.6476 66.9358 5.56 64.28
Na Diklofenak 2.25% 6 61.9767 12.66065 5.16869 48.6901 75.2632 38.46 75.00
Total 30 62.4780 18.99493 3.46798 55.3852 69.5708 5.56 92.85
Menit 225 CMC Na 6 77.7000 8.26213 3.37300 69.0294 86.3706 70.00 92.85
EEM 200 mg/Kg BB 6 60.9150 10.70584 4.37064 49.6799 72.1501 47.06 75.00
EEM 300 mg/Kg BB 6 58.5700 21.29170 8.69230 36.2257 80.9143 33.33 92.85
EEM 400 mg/Kg BB 6 41.7083 21.21609 8.66143 19.4434 63.9733 5.56 64.28
Na Diklofenak 2.25% 6 58.2633 11.74818 4.79617 45.9344 70.5923 38.46 75.00
Total 30 59.4313 18.60174 3.39620 52.4853 66.3773 5.56 92.85
Menit 240 CMC Na 6 73.6400 10.78287 4.40209 62.3241 84.9559 60.00 92.85
EEM 200 mg/Kg BB 6 58.4417 9.86717 4.02826 48.0867 68.7966 47.06 75.00
EEM 400 mg/Kg BB 6 39.4767 19.00883 7.76032 19.5281 59.4252 5.56 57.14
Na Diklofenak 2.25% 6 51.1817 10.82595 4.41967 39.8205 62.5428 38.46 66.67
Total 30 54.6870 17.43071 3.18240 48.1783 61.1957 5.56 92.85
Menit 255 CMC Na 6 71.0617 8.54233 3.48739 62.0970 80.0263 60.00 85.71
EEM 200 mg/Kg BB 6 57.0533 7.33168 2.99315 49.3592 64.7475 47.06 66.67
EEM 300 mg/Kg BB 6 47.2050 14.83861 6.05784 31.6328 62.7772 26.67 71.43
EEM 400 mg/Kg BB 6 36.1350 17.09985 6.98099 18.1898 54.0802 5.56 50.00
Na Diklofenak 2.25% 6 44.9683 8.84439 3.61071 35.6867 54.2499 37.50 58.33
Total 30 51.2847 16.45311 3.00391 45.1410 57.4284 5.56 85.71
Menit 270 CMC Na 6 68.2850 11.43654 4.66895 56.2831 80.2869 50.00 85.71
EEM 200 mg/Kg BB 6 56.1733 8.91661 3.64019 46.8159 65.5307 41.78 66.67
EEM 300 mg/Kg BB 6 47.2050 14.83861 6.05784 31.6328 62.7772 26.67 71.43
EEM 400 mg/Kg BB 6 36.1350 17.09985 6.98099 18.1898 54.0802 5.56 50.00
Na Diklofenak 2.25% 6 44.9683 12.21545 4.98694 32.1490 57.7877 30.77 61.54
Total 30 50.5533 16.54489 3.02067 44.3754 56.7313 5.56 85.71
Menit 285 CMC Na 6 67.0967 11.41480 4.66007 55.1176 79.0758 50.00 85.71
EEM 200 mg/Kg BB 6 57.0533 7.33168 2.99315 49.3592 64.7475 47.06 66.67
EEM 300 mg/Kg BB 6 44.9050 12.85454 5.24785 31.4150 58.3950 26.67 64.30
EEM 400 mg/Kg BB 6 34.9433 16.16389 6.59888 17.9804 51.9063 5.56 50.00
Na Diklofenak 2.25% 6 42.5383 11.74612 4.79533 30.2115 54.8651 30.77 61.54
Total 30 49.3073 16.22108 2.96155 43.2503 55.3644 5.56 85.71
Menit 300 CMC Na 6 64.7033 11.77817 4.80842 52.3429 77.0638 50.00 85.71
EEM 200 mg/Kg BB 6 58.5317 11.21490 4.57846 46.7623 70.3010 46.66 75.00
EEM 300 mg/Kg BB 6 43.7117 10.87365 4.43915 32.3005 55.1229 26.67 57.14
EEM 400 mg/Kg BB 6 34.9433 16.16389 6.59888 17.9804 51.9063 5.56 50.00
Na Diklofenak 2.25% 6 42.5383 11.74612 4.79533 30.2115 54.8651 30.77 61.54
Total 30 48.8857 16.12242 2.94354 42.8655 54.9059 5.56 85.71
Menit 315 CMC Na 6 59.3417 14.94144 6.09982 43.6616 75.0217 40.00 85.71
EEM 200 mg/Kg BB 6 55.9600 8.40913 3.43301 47.1352 64.7848 46.66 66.67
EEM 300 mg/Kg BB 6 37.8250 13.51652 5.51810 23.6403 52.0097 20.00 57.14
EEM 400 mg/Kg BB 6 30.8233 14.67896 5.99266 15.4187 46.2280 5.56 43.75
Menit 330 CMC Na 6 59.3417 14.94144 6.09982 43.6616 75.0217 40.00 85.71
EEM 200 mg/Kg BB 6 53.8717 8.75647 3.57481 44.6823 63.0610 41.78 66.67
EEM 300 mg/Kg BB 6 37.8250 13.51652 5.51810 23.6403 52.0097 20.00 57.14
EEM 400 mg/Kg BB 6 30.8233 14.67896 5.99266 15.4187 46.2280 5.56 43.75
Na Diklofenak 2.25% 6 38.8233 11.29531 4.61129 26.9696 50.6770 25.00 53.84
Total 30 44.1370 16.14164 2.94705 38.1096 50.1644 5.56 85.71
Menit 345 CMC Na 6 56.8750 12.49517 5.10113 43.7621 69.9879 40.00 78.60
EEM 200 mg/Kg BB 6 52.4817 6.75079 2.75600 45.3971 59.5662 41.78 58.33
EEM 300 mg/Kg BB 6 36.5417 14.63924 5.97645 21.1787 51.9046 20.00 57.14
EEM 400 mg/Kg BB 6 29.7817 13.77192 5.62236 15.3289 44.2344 5.56 42.85
Na Diklofenak 2.25% 6 36.1533 11.50068 4.69513 24.0841 48.2226 23.08 53.84
Total 30 42.3667 15.48308 2.82681 36.5852 48.1481 5.56 78.60
Menit 360 CMC Na 6 56.8750 12.49517 5.10113 43.7621 69.9879 40.00 78.60
EEM 200 mg/Kg BB 6 52.4817 6.75079 2.75600 45.3971 59.5662 41.78 58.33
EEM 300 mg/Kg BB 6 35.3517 12.80528 5.22773 21.9134 48.7900 20.00 50.00
EEM 400 mg/Kg BB 6 24.7017 13.49559 5.50955 10.5389 38.8644 5.56 37.50
Na Diklofenak 2.25% 6 36.1533 11.50068 4.69513 24.0841 48.2226 23.08 53.84
Lampiran 12. Hasil ANAVA secara SPSS persen radang
ANOVA
Sum of Squares df Mean Square F Sig.
Menit 15 Between Groups 76.439 4 19.110 1.272 .307
Within Groups 375.697 25 15.028
Total 452.136 29
Menit 30 Between Groups 220.832 4 55.208 3.352 .025
Within Groups 411.756 25 16.470
Total 632.588 29
Menit 45 Between Groups 1637.769 4 409.442 9.721 .000
Within Groups 1052.994 25 42.120
Total 2690.763 29
Menit 60 Between Groups 2883.184 4 720.796 13.173 .000
Within Groups 1367.994 25 54.720
Total 4251.178 29
Menit 75 Between Groups 3569.032 4 892.258 10.682 .000
Within Groups 2088.224 25 83.529
Total 5657.256 29
Menit 90 Between Groups 5874.314 4 1468.579 9.219 .000
Within Groups 3982.423 25 159.297
Total 9856.737 29
Menit 105 Between Groups 7193.976 4 1798.494 13.644 .000
Within Groups 3295.514 25 131.821
Total 10489.490 29
Menit 120 Between Groups 9223.025 4 2305.756 17.487 .000
Within Groups 3296.362 25 131.854
Total 12519.387 29
Menit 135 Between Groups 7161.711 4 1790.428 10.584 .000
Within Groups 4229.006 25 169.160
Total 11390.718 29
Total 11807.591 29
Menit 165 Between Groups 6461.208 4 1615.302 6.589 .001
Within Groups 6128.993 25 245.160
Total 12590.200 29
Menit 180 Between Groups 5941.864 4 1485.466 6.139 .001
Within Groups 6048.924 25 241.957
Total 11990.788 29
Menit 195 Between Groups 5010.504 4 1252.626 5.200 .003
Within Groups 6021.963 25 240.879
Total 11032.467 29
Menit 210 Between Groups 4580.096 4 1145.024 4.866 .005
Within Groups 5883.315 25 235.333
Total 10463.411 29
Menit 225 Between Groups 3912.938 4 978.234 3.995 .012
Within Groups 6121.781 25 244.871
Total 10034.719 29
Menit 240 Between Groups 3797.348 4 949.337 4.734 .006
Within Groups 5013.716 25 200.549
Total 8811.065 29
Menit 255 Between Groups 4262.756 4 1065.689 7.426 .000
Within Groups 3587.687 25 143.507
Total 7850.444 29
Menit 270 Between Groups 3577.730 4 894.432 5.128 .004
Within Groups 4360.535 25 174.421
Total 7938.265 29
Menit 285 Between Groups 3887.912 4 971.978 6.493 .001
Within Groups 3742.667 25 149.707
Total 7630.579 29
Menit 300 Between Groups 3628.149 4 907.037 5.800 .002
Within Groups 3909.892 25 156.396
Total 7538.041 29
Menit 315 Between Groups 3626.105 4 906.526 5.725 .002
Total 7584.427 29
Menit 330 Between Groups 3427.654 4 856.914 5.189 .003
Within Groups 4128.373 25 165.135
Total 7556.028 29
Menit 345 Between Groups 3262.340 4 815.585 5.526 .002
Within Groups 3689.706 25 147.588
Total 6952.046 29
Menit 360 Between Groups 4228.862 4 1057.216 7.773 .000
Within Groups 3400.371 25 136.015
Lampiran 13. Hasil analisis deskriptif AUC dengan SPSS
Descriptives
AUC
N Mean Std. Deviation Std. Error
95% Confidence Interval for Mean
Minimum Maximum Lower Bound Upper Bound
Cmc Na 0,5% 6 2.2288E4 3613.97945 1.47540E3 18445.4362 26030.7138 17625.00 28708.95
EEM 200 mg/kg bb 6 1.8538E4 2647.64023 1.08089E3 15714.8111 21271.8672 14855.40 22062.30
EEM 300 MG/KG bb 6 1.4025E4 6884.87719 2.81074E3 5086.9868 19537.4578 457.56 21053.25
EEM 400 mg/kg bb 6 1.1040E4 4131.09980 1.68651E3 6682.7930 15353.4397 4042.30 14671.88
Natrium Diklofenak 2.25% 6 1.3732E4 3020.20786 1.23299E3 10542.5862 16881.6138 9690.45 17937.38
Lampiran 15. Gambar alat yang digunakan
(a) (b)
Lampiran 15. (Lanjutan)
(e) (f)
Keterangan:
(a) Timbangan hewan
(b) Rat strainer
Lampiran 16. Gambar penambahan volume kaki tikus
(a)
(b)
Keterangan:
