© Research India Publications http://www.ripublication.com
Accumulation of Pb, Cu and Zn in Organ System of
Parang Fish (Chirocentrus dorab) and Biang Fish
(Setipinna paxtoni) from Meranti Islands, Sumatera
Indonesia
Yusni Ikhwan Siregar1) Department of Marine Science,
Faculty of Fisheries And Marine Science Riau University, Pekanbaru
Abstract
The presence of heavy metals in coastal ecosystem have deleterious effect on ecosystem, due to that of non-degradable properties and long half-lives. The accumulation rate in the tissue and internal organ of fish are species-dependent and are related to the mechanism of metabolism and detoxification. The present paper aims at gaining information on distribution and accumulation of Pb, Cu and Zn in fish of organ system of locally consumed fish, Parang Parang as well as Biang fish.
Sample of Parang parang (Chirocentrus dorab) and Biang (Setipinna paxtoni) were collected from fish catch of trap-net). Fish sample were kept in ice box prior to analyzing in Laboratorium. Pb, Cu and Zn were analyzing following Hutagalung,1997 by using AAS Perkin Elmer (Model 3110).
It revealed that both concentration of nonesential Pb, Cu and esential Zn, of the gill were higher than that in fish muscle and fish bone. It also apperared that essential heavy metal, Zn, is higher in organ system of both fishes. Zn in gill accounted three to four fold higher than that of fish muscle and fish bone. In general those metals were accumulated 20 to 80 folds than that of in seawaters. Based on FAO/WHO Expert Committe on Food Additives (2004), however, the level of heavy metals were in the range of safe for consumption. Keywords: heavy metals, accumulation, fish, tropical coastal ecosystem.
Heavy metals have been reported to contribute to the anthropogenic contamination of marine ecosystem. Some of them are esential ( Zn, Cu) to the life processes of organism,; while others are toxic (Pb, Hg and Cd) even at low concentration. Uptake of heavy metals in fish are considerred through penetration passively via gill memberane, skin and via food. The presence of heavy metals in oceans have deleterious effect due to that of nondegradable properties and long half lives. Many heavy metals are accumulated in tropical coastal fish (Siregar 2009; Siregar 2011; Amin, 2009, 2010, 2013) . The accumulation in the tissue and organ system of fish are species –dependent and are related to the mechanism of metabolism and detoxification.
Contamination of xenobiotic especially heavy metals in coastal ecosystem were continuously reported in relation to fast development of adjacent coastal area. This anthrophogenik pollutant enter coastal ecosystem and undergoes hidrological processes that also interact with biological prosess through food chain. Aquatic vertebrate such as fish, through passive and active mechanisme may accumulate tha heavy metals. Fish uses a variety of homeostasis strategies to limit the accumulation of metals that coudl elicit toxic effects (Jakimska, 2011). In the metabolism it is internal organ (liver), the site of detoxification, accumulation, biotranformation, and enhance elimination (Fernandes 2007; Triebskorn 1997).
Kepulauan Meranti District of Riau Province consists of small islands namely Tebing Tinggi, Ransang and Merbau. As a fast growing urbanization of the district, along the coast of the islands has developed many sago processing plants, harbor, and city infrastructure which presumably brought about variety of pollution to coastal waters. Selat Air hitam is a strait of pollution address which heavily used for sea traffic, water front city development, marine fish culture, and fishing ground for traditional people. The present paper aims at gaining information on distribution and accumulation of Pb, Cu and Zn in fish of organ system of Parang Parang fish as well as Biang fish, the common consumed fish locally in Kepulauan Meranti District, Riau Indonesia.
2. MATERIALS AND METHODS
Fish sample were collected from traditional trapnet, so-called Gombang, along Selat Air Hitam Meranti Island. Two sample stations were established purposively along the straits (Fig. 1). Station 1 represent area of adjacent to domestic waste source and industrial area of Selat Air Hitam of Meranti , while station 2 with three subsampling station, represent of natural area since far away from dense population city and adjacent a good mangrove vegetation.
Figure 1. Map of Sampling Stations in Selat Air Hitam of Meranti District.
The fish of various size were sampled and kept in a cool boxes prior to the destruction of the sample in Laboratorium. The organ system of fishes including gills, muscle, bone and internal organ were seperated befor analyzing in AAS Perkin Elmer (Model 3110).
3. RESULT AND DISCUSSION
It revealed that consentration of Pb, Cu and Zn in organ system of Parang-parang fish
(C. dorab) and Biang fish (S paxtoni) varied widely, as showed in Table 1.
organ and fish bone) of Parang fish (C dorab) (n = 10) 1.a, and Biang fish (S paxtoni) 1.b
1.a. Parang fish Organ Consentration (µg/g) Pb Cu Zn Gill 8,60± 1,43 9,25± 2,30 40,66± 7,31 Muscle 1,86± 0,32 1,75± 0,31 9,42 ± 2,02 Bone 4,72± 0,48 4,10± 0,59 20,10 ± 1,62 Seawater 0,017 0,107 0,107 1.b Biang fish Organ Concentrations (µg/g) Pb Cu Zn Gill 7,89± 0,43 10,62± 0,08 35,34± 3,84 Muscle 2,45± 0,15 3,38± 0,42 14,19 ± 0,67 Bone 3,74± 0,00 5,20± 0,53 15,85 ± 4,50 Seawater 0,107 0,107 0,107______
Table 2. Summary of statistical analysis of heavy metals content in organ system of Fish.
Metal Organ Gills Muscle Internal Fish Bone
Pb Muscle -
Daging 0,001** -
Internal O 0,001** 0,820tn -
Bone 0,009** 0,024* 0,030* -
Muscle 0,004** - I ternal 0,004** 0,912tn - Bone 0,014* 0,127tn 0,145tn - Zn Gill - Muscle 0,001** - Internal 0,001** 0,949tn - Bone 0,007** 0,054tn 0,058tn - Note * = p < 0,05 (significant) ** = p <0,01 (highly significant) tn (non-signoificant)
It revealed that the gill accumulate toxic metal Pb and Cu highly since the organ function related to respiratory mechanism of fishes. Statistical analysis (ANOVA) showed that concentration/accumulation of both Pb and Cu differs (p<0.05) siginificantly between organ. Gill appeared acummulated all non-essential heavy metals(Zn) the highest Table 1. Comparison of heavy metal concentration of Pb, Cu and Zn in organ system of Parang and Biang fish is illustrated in Figure 2,3 and 4.
Figure 2. Consentration of Pb in Parang fish (C. Dorab) and Biang Biang Fish (S. paxtoni ) 0 1 2 3 4 5 6 7 8 9 10
Gill Muscle Bone
Co n ce n tr ation o f Pb ( µ g /g ) Fish Organ Parang Fish Biang Fish
Figure 3. Consentration of Cu in Paran Parang fish (C. Dorab) and Biang Biang Fish (S. paxtoni )
Figure 4. Consentration of Zn in Paran Parang fish (C. Dorab) and Biang Biang Fish
(S. paxtoni )
Apparently, there was no different consentration of Pb, Cu and Zn between sampling stations. The body of water in Selat air Hitam tend to be homogen all year long since the two station were closely related in a semi-enclosed hydrooceanic system. Comparison of heavy metals in two station are shown in Figure 5 (Parang Fish) and Figure 6 for Biang Biang Fish.
0 2 4 6 8 10
Gill Muscle Bone
Co n ce n tr ation o f Cu ( µg /g ) Fish Organ Parang Fish Biang Fish 0 5 10 15 20 25 30 35 40 45
Gill Muscle Bone
Co n ce n tr ation o f Z n ( µg /g ) Fish Organ Parang Fish Biang Fish
Figuire 5. Comparison of Pb, Cu and Zn in Parang fish (C. Dorab) sampled from two stasiun in Selat Air Hitam Kepulauan Meranti, Riau
Figuire 6. Comparison of Pb, Cu and Zn in Biang Biang fish (S. paxtoni ) sampled from two stasiun in Selat Air Hitam Kepulauan Meranti, Riau
The main heavy metal sources were attributed to domestic sewage, agricultural effluent and industrial that develop in the regions. Inter-species differences of heavy metal accumulation were attributed to habitat differences as well as food habit of coastal fish. Level of Zn in gill accounted three to four fold higher than that of fish
0.00 5.00 10.00 15.00 20.00 25.00 30.00 35.00 40.00 45.00 Pb Cu Zn C o n se n tr at io n ( µ g/ g) Metals St. I St. II 0.00 5.00 10.00 15.00 20.00 25.00 30.00 35.00 40.00 Pb Cu Zn Co n sen tr ation ( µ g/ g) Metals St. I St. II
metals were accumulated 20 to 80 folds than that of in seawaters. Based on FAO/WHO Expert Committe on Food Additives (2004), however, the level of heavy metals were in the range of safe for human consumption.
REFERENCES
[1] Amin, B. 2002. Lokan (G. coaxans) sebagai Biomonitoring Logam Berat di Muara Sei Jang Tanjung Pinang Timur Riau. Jurnal Perikanan dan Ilmu Kelautan. Vol. 7.(52-61)
[2] Amin, B., Ismail, A dan Yap, C.K., 2008b. Heavy metal concentrations in sediments and intertidal gastropod Nerita lineata from two opposing sites in the Straits of Malacca. Wetland Science 6(3): 411 – 421.
[3] Arisandi, P, 2004. Mewaspadai Bahaya Timbal di Surabaya. http// www.ecoton.or.id.
[4] BSN. 2009. Batas Maksimum Cemaran Logam Dalam Pangan. Penerbit: Standart Nasional Indonesia. Jakarta
[5] Budiasih, K. S. 2011. Interferensi Ion Cd (II) dan Hg (II) Terhadap Biofungsi Persenyawaan Zn (II) pada Tubuh Manusia. Prosiding Seminar Nasional Penelitian, Pendidikan dan Penerapan MIPA, Fakultas MIPA, Universitas Negeri Yogyakarta.
[6] Chahaya, I. S. 2003. Ikan Sebagai Alat Monitor Pencemaran.Bagian Kesehatan Lingkungan. Fakultas Kesehatan Masyarakat. Universitas Sumatera Utara.
[7] Connel, W.D dan G. J. Miller. 1995. Kimia dan Ekotoksikologi Pencemaran. Penerbit Universitas Indonesia. Jakarta.
[8] Dahuri, R., J. Rais, S.P. Ginting dan M.J. Sitepu. 2001. Pengelolaan Sumberdaya Wilayah Pesisir dan Lautan Secara Terapdu. Pradya Paramita. Jakarta. 167 hal.
[9] Darmono, 2001. Lingkungan Hidup dan Pencamaran, Hubungan dengan Toksikologi Senyawa Logam. Universitas Indonesia Press, Jakarta. 167 hal
[10] Fairy, T. I. 2008. Kandungan Logam Berat Pb dan Cu pada Ikan Gulama
(Sciaenaa ruselli) Dengan Ukuran yang Berbeda. Skripsi Ilmu Kelautan Faperika Universitas Riau. Pekanbaru (Tidak diterbitkan).
[11] FAO/WHO, 2004. Summary of Evaluations Ferformade by the Jint
FAO/WHO Expert Committe of Food Additives (JECFA 1956-2003), ILSI Press International Life Sciences Institute.
[12] Hambidge, K. M. and N. F. Krebs. 2007. “Zinc deficiency: a special
challenge”. J. Nutr. 137. Karakteristik Seng. Wikipedia (eds). http://id.wikipedia.org/wiki/seng. Dikunjungi tanggal 25 September 2014.
[13] Harja, E. 2007. Kandungan Logam Berat Pb, Cu, Zn di perairan Bungus Teluk
Kabung Padang. Skripsi Fakultas Perikanan dan Ilmu Kelautan Universitas Riau. Pekanbaru. (Tidak diterbitkan)
Dalam D. H. Kunarso dan Ruyitno (eds). Status Pencemaran Laut Indonesia dan Teknik Pemantauannya. PPPO-LIPI. Jakarta
[15] Julianto, M.T.B., 2008. Pencemaran Logam Berat Juga Bisa Terdapat Dalam
Makanan. http://www. Berbagi sehat.com.
[16] Karadede, H. Oymak, S.A, Unlu. E., 2004. Heavy metals in mullet, Liza abu,
and catfish, Silurus triotegus, from the Ataturk Dam Lake (Eupharates), Turkey. Environmental International 30: 183 – 188.
[17] Khaled A., 2004. Heavy metal concentrations in certain tissues of five
commercially important fishes from El-Mex Bay, Al-Exandria, Egypt. Pp 1 – 11.
[18] Kusumahadi KS. 1998. Konsentrasi Logam Berat Pb, Cr dan Hg dalam Badan
Air dan Sedimen serta Hubungannya dengan Keanekaragaman Plankton, Benthos dan Ikan di Sungai Ciliwung. [disertasi]. Bogor: Program Pascasarjana, Institut Pertanian Bogor.
[19] Durala M, M. Ziya L G, Argun A Ö., 2007. Investigation of heavy metal
levels in economically important fish species captured from the Tuzla lagoon, Turkey. Food Chemistry. Volume 102, Issue 1, 2007, Pages 415–421
[20] Menteri Negara Kependudukan dan Lingkungan Hidup, 2004. Surat
Keputusan Nomor: Kep.51/MEN-KLH/II/2004 Tentang Pedoman Penetapan Baku Air Laut Untuk Biota Laut. Sekretaris Menteri Negara Kependudukan dan Lingkungan Hidup, Jakarta. 51 hal.
[21] Mihardja, D K. Putri, M. 1999. Pemodelan Sirkulasi Air Laut dan Penyebaran
Logam Berat Cadmium (Cd) di Kolam Pelabuhan Tanjung Priok. Program Studi Oseanografi. Fakultas Matematika dan Ilmu Pengetahuan Alam. Institut Teknologi Bandung.
[22] Murtiani, L., 2003. Analisis Kadar Timbal (Pb) pada Ekstrak Kerang Darah
(Anadara granosa L) di Muara Sungai Tambak Oso Sedati-Sidoarjo. Skripsi. Surabaya: Universitas Negeri Surabaya. (Tidak Dipublikasikan)
[23] Nasution, A. F. 2008. Bahaya Timbal Hitam.
http://www.fishyforum.comfishysalt fishyronment 11857 bahaya timbal hitam. Html. Dikunjungi tanggal 12 Januari 2013.
[24] Nicula, M., P. Negrea, I. Gergen, M. Harmanescu, Gogoasai, and M. Lunca,
2008. Mercury Bioaccumulation In Tissues Of Fresh Water Fish Carassius auratus gibelio (Silver Crucian Carp) After Chronic Mercury Intoxication. Unversitatea Stiintifice. Vol.52 ; 676 – 679.
[25] Nybakken. J. W. 1992. Biologi Laut: Studi Pendekatan Ekologis
(diterjemahkan oleh H. M. Eidman, dkk). UGM Press . Yogyakarta.
[26] Olaifa, F.E., A.K Olaifa, A.A Adelaja dan A.G Owolabi, 2004. Heavy Metal
Contamination of Clarias gariepinus from a Lake and Fish Farm in Ibadan, Nigeria. African Journal of Biomedical Research Vol.7: 145 – 148.
[27] Pagoray, H. 2001. Kandungan Merkuri dan Kadmium Sepanjang Kali Donan
Kawasan Industri Cilacap. Artikel Frontir Nomor 333. http://www.ummul.ac.id.
[28] Palar, H., 2004. Pencemaran dan Toksikologi Logam Berat. Rineka Cipta,
Makanan No. 03725/B/SK/89 tentang Batas Maksimum Cemaran Logam dalam Ikan dan Hasil Olahannya. Jakarta
[30] Purnomo, T. 2007. Analisis Kandungan Timbal (Pb) pada Ikan Bandeng
(Chanos chanos Forsk) di Tambak Kecamatan Gresik. FMIPA-Biologi Universitas Surabaya.
[31] Putra, S. 2010. Kandungan Logam Berat dan Bahan Organik Pada Sedimen di
Periran Muara Sungai Asahan Tanjung Balai Provinsi Sumatera Utara. Skripsi Fakultas Perikanan dan Ilmu Kelautan Universitas Riau. Pekanbaru. (tidak diterbitkan).
[32] Razak, H. 1987. Pengaruh Logam Berat Terhadap Lingkungan. Warta Oseana
6 (2) : 9-15.
[33] Riani, Etty. 2004. Dampak Bahan Pencemar terhadap Kecacatan dan
Kepunahan Organisme Laut. http://www.wwf.or.id/Default.php?ID=570. 28 Maret 2005.
[34] Saanin, 1985. Taksonomi dan Kunci Identifikasi Ikan. Binacipta. Bogor. 508
hal
[35] Salami, Indah RS; S Rahmawati; AP Kristijarti dan AT Yusuf. 2008. Pengaruh
Logam Berat Tembaga Pada Ikan Nila (Oreochromis niloticus)dan Pengaruh Depurasinya. Jurnal Penelitian Perikanan 11 (1): 49-58.
[36] Sastrawijaya, A. R. 1991. Pencemaran Lingkungan. Rineka Cipta, Jakarta. 274
hal.
[37] Siregar, Y S, 2009. Faktor Konsentrasi Pb, Cd, Cu, Ni, Zn Dalam Sedimen
Pesisir Kota Dumai. Maspari Jurnal Vol I No. 1 (1-10).
[38] Supriharyono, 2000. Pelestarian dan Pengelolaan Sumberdaya Alam di
Wilayah Pesisir Tropis. Gramedia Pustaka Umum. Jakarta. 246 hal.
[39] Waldichuk, M. 1974. Some Biologi Concentration in Metal Pulution In
F.J.Verberg (eds). Pollution and Physioology of Marine Organism. Academic Press. London. P1-15.
[40] WHO, 1989. Technical Report Series No. 776. Gevana.
[41] Windarti. 2007. Fisiologi Ikan. Universitas Riau press. Pekanbaru.
[42] Wikipedia. 2014. Kabupaten Kepulauan Meranti.
http://wikipedia.org/wiki/Kabupaten_kepulauan_meranti. 25 September 2014.
[43] Yap, C. K. Ismai, A. Tan, S.G. and Umar, H. 2003. Concentration of Cu and
Pb in the Offshore and Intertidal Sediments of the West Coast of Penensular Malaysia. Environment International. 20:267-479.
[44] Yilmaz A. B., 2003. Levels of heavy metals (Fe, Cu, Ni, Cr, Pb, and Zn) in
tissue of Mugil cephalus and Trachurus mediterraneus from Iskenderun Bay, Turkey Environmental Research. Volume 92, Issue 3, July 2003, Pages 277– 281
