The effect of feeding serum on bovine blastocyst development

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Symposium "Reproductive for Improved Southeast Asia"

of feeding

serum

on

bovine

blastocyst development

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Institute for Animal Production, PO BOX 221, Indonesia of Clinic, Reproduction, and Pathology, Faculty of

University,

of Wisconsin, USA

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main of this experiment is to obtain information on proper technique for in production of Holstein x Bali cattle hybrid embryos using USA Holstein oocytes collected

and fertilized with spermatozoa of Bali or bulls. Holstein Brahman hybrid embryos using of USA Brahman bulls and Hol-

stein embryo production. All viable embryos were frozen and brought to Indonesia and some of

them had been transferred to FH recipients in Indonesia.

These embryos may be used for improving the genetic value of the Indonesian dairy cattle. There

is transportation constraint in importing oocytes from USA. It takes more than 24 hours to bring it to Indonesia. In fact, maturation of and readiness to be fertilized normally requires only

24 hours

in

5% incubator at Therefore, this research is needed to study the of gonadotropin hormone and time for oocytes maturation and readiness to be fertilized at more than 24 hours. If this problem could be solved the importation of oocytes

tion was used for fertilization of oocytes.

Sperm

and oocytes were incubated in fertiliza-

tion medium (TALP) for 20 hours. All zygotes were cultured in medium and fed on day 4, 5 and 6 (as treatment A, B and C, respectively) with 5 culture up to blastocyst. Counting of expanded blastocyst, degenerated embryo and cleavage followed the method of and (1 994).

this showed that the percentages cleavage, unfertilized ova were not different among treatments but degenerated embryos and

were used as recipients, estrous synchronization using CIDR (Controlled Internal Drug Re-

lease) implanted for 12 days, but seven cows which had good of corpora lutea were transplanted with the embryos (three embryos per recipient) and in pregnancy of

three recipients (pregnancy rate 42.86 Unfortunately two of them aborted only one male) was alive with 45 kg

birth

weight (hybrid FH x Brahman). the second trial, 32 cows were prepared as recipients synchronization with implanted for 12 days, but only nine cows had good of corpora Three recipients were transplanted with

IVF

Asia Link Symposium Biotechnology for Improved Asia"

were transplanted with the in embryos (two embryos per cow) and three them became pregnant (pregnancy rate Three days after were born. however. the calves died due

to problem of the dams.

Table The of feeding serum on embryo development

--

Feeding serum of (%) oocytes

. . . . .

--Cleavage

zed a Day4

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C st Expblast

Embryos development

+Day-5

Figure I . The effect of feeding serum on

It is generally concluded U S A originated oocytes could be matured and

fertilized

in after a period o f more than 14 hours up to 36 hours with without hormone supplement in of its medium. Embryos produced by this process could reach the blastocyst and expanded

blastocyst stages and will be transferred to recipients in Indonesia.

It would appropriate to recommend that further cooperarcd action research and implementa- tion in this reproduction should be conducted genetically superior

of Holstein from USA and fertilized with spermatozoa from developed countries in