KARAKTERISTIK RESPON IMUN TERHADAP SARS-COV-2
Fedik A. Rantam
Research Center for Vaccine Technology and Development, Institute of Tropical Diseases
Universitas Airlangga
OUTLINE
■ Pendahuluan
■ Variant Coronavirus
■ Response imun
■ Karakteristik antibodi
■ Reaktifitas antibodi
■ Antibodi netralisasi
■ Penutup
SARS-CoV-2,Rantam et al. 2021
PENDAHULUAN
■ SARS-CoV-2 adalah virus corona penyebab COVID-19 termasuk genus beta-Coronavirus
■ Mempunyai variant alpha, beta, gama, delta, epsilon yang sudah ditemukan dibeberapa negara UK, South of Africa, Brazil, India dan Indonesia
■ Variant Delta-Coronavirus saat ini menyebabkan penyebaran dan kematian sangat tinggi
■ Indonesia menyebabkan kematian kurang lebih 79.032
■ Indonesia telah dilakukan vaksinasi sekitar 16,1 % dari populasi seluruh Indonesia
■ Mempunyai karakteristik response imune yang beragam
VARIANTS OF COVID 19
■ The World Health Organisation has classified each emerging variant as either a Variant of Concern (VOC) or a Variant of Interest (VOI). The Alpha,
Beta, Gamma and Delta variants fall under Variants of Concern. Whereas the Eta, Iota, Kappa and Lambda fall under Variants of Interest.
■ Delta is the name for the B.1.617.2. variant, a SARS-CoV-2 mutation that originally surfaced in India. The first Delta case was identified in December 2020, and the strain spread rapidly, soon becoming the dominant strain of the virus in both India and then Great Britain.
■ Di Indonesia Varian delta ditemukan sejak 17 maret 2021 oleh Eijkman
sampel dari makasar. Sementara varian alfa dilaporkan di Indonesia oleh
LITBANGKES sejak 2 maret 2021 (GISAID)
CORONAVIRUS VARIANTS
Medical express,2020
B1.351=beta
B1.617=delta
B1.1.7=alfa B1.429=epsilon
A
ISOLASI NEW VARIANTS SARS-CoV-2 DARI PASIEN COVID-19
A
Pasase 3 SARS- CoV-2 FIRST WAVE
Pasase 1, SARS-CoV- 2 SECOND WAVE
Pasase 1,
Negative SARS-
CoV-2
RESEARCH ARTICLE
Ch ar act er i zat i o n of SARS-CoV-2 East Java i sol at e, I n don esi a [v er si on 1; p eer r ev i ew : aw ai t i n g p eer r ev i ew ]
Fedik Abdul Rant am
1,2, Cit a Rosit a Sigit Pr akoesw a
3, Dam ayant i Tindu h
4, Jusak Nugr aha
5, Helen Susilowat i
1, Andi Yasm in Wijaya
6,
Ni Nyom an Tr i Puspanin gsih
7, Dwiyant i Puspit asar i
8, Dom inicus Husada
8, Nen eng Dew i Kur n iat i
9, Ar yat i Ar yat i
51Resear ch Cen t er f or Vaccin e Technolog y and Develop m en t , I n st it ut e of Tr op ical Disease, Air langg a Univer sit y, Su r abaya, East Java, 60132, I nd on esia
2Vir olog y and Im m u n ology Lab or at or y, Dep ar t m en t of M icr ob iology, Facu lt y of Vet er in ar y M edicin e, Air lan g ga Un iver sit y, Su r ab aya, East Java, 60132, In don esia
3Pr of essioal Ed ucat ion an d Resear ch , Dr . Soet om o Gen er al Acad em ic Hospit al, Facu lt y of M ed icin e, Air langg a Un iver sit y, Sur abaya, East Java, 60132, In don esia
4Resear ch and Developm en t Boar d , Dr . Soet om o Gener al Hospit al, Su r ab aya, East Java, 60132, In d on esia
5Clinical Pat h olog y Dep ar t m ent , Dr . Soet om o Gener al Hosp it al, Facu lt y of M edicine, Air lan g ga Univer sit y, Su r abaya, East Java, 60132, I nd on esia
6Facu lt y of M edicin e, Air lan g ga Un iver sit y, Su r ab aya, East Java, 60132, I nd on esia
7Bior esou r ce En gin eer ing Gr ou p in Resear ch Cen t er for Bio-M olecule En gin eer in g (BIOM E), Air langg a Un iver sit y, Sur ab aya, East Java, 60132, In don esia
8Ped iat r ics Depar t m ent , Dr . Soet om o Gener al Hospit al, Su r abaya, East Java, 60132, Ind onesia
9Clinical M icr obiology Depar t m en t , Dr . Soet om o Gen er al Hospit al, Su r ab aya, East Java, 60132, In d on esia
Fi r st p u b l i sh ed : 16 Jun 2021, 10:480
ht t p s:/ / doi.or g / 10.12688/ f 1000r esear ch .53137.1 Lat est p u b l i sh ed : 16 Ju n 2021, 10:480 ht t p s:/ / doi.or g / 10.12688/ f 1000r esear ch .53137.1
v 1
Ab st r act
Back g r ou n d : Incident s of SARS-CoV-2 in East Java incr eased st eadily, and it becam e t he second epicent er in Indonesia. The COVID-19 pandem ic caused a dir e m ult isect or al cr isis all ar ound t he wor ld. This st udy invest igat es and char act erizes local isolat es from East Java, Indonesia.
M et h od s: There wer e 54 pat ient s suspect ed wit h SARS-COV-2 infect ion and 27 pat ient s wer e COVID-19 posit ive. Virus
isolat es wer e obt ained from COVID-19 inpat ient s’ nasophar yngeal sw abs at t he Dr Soet om o Teaching Hospit al, Surabaya. Ther e wer e only t h r ee isolat es (# 6, # 11, # 35) wit h good gr owt h
char act er ist ics. Serial blind passage and cyt opat hic effect obser vat ion in t he Ver o E6 cell line were perfor m ed for vir us isolat ion.
Con fir m at ion of t he SARS-CoV-2 infect ion was pr oven by m eans of r ever se t r anscr ipt ase-polym er ase chain r eact ions using SARS-CoV-2 specific pr im er s, scanning elect r on m icr oscopy, and scanning t r ansm ission elect r on m icr oscopy exam inat ion . Whole genom e sequencing was per for m ed u sing ARTIC pr ot ocol. Fur t her m or e, SARS- CoV-2 char act er izat ion was ident ified t hrough a west er n blot using
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F1000Resear ch 2021, 10:480 Last u pd at ed : 15 JUL 2021
