81
LAMPIRAN A
82
LAMPIRAN B
83
LAMPIRAN C
HASIL PERHITUNGAN SUSUT PENGERINGAN, KADAR ABU,
DAN KADAR SARI LARUT ETANOL
Hasil perhitungan penetapan susut pengeringan serbuk
Replikasi Hasil Susut
Pengeringan(%)
1 8,2
2 8,5
3 8,5
Rerata 8,4
Rata-rata 8,4%
Hasil Perhitungan Penetapan Kadar Abu
No serbuk (g) Berat Berat krus kosong (g) Berat krus + abu (g) Kadar abu (%)
1 2,016 20,269 20,348 3,919
2 2,018 20,269 20,351 4,063
3 2,019 20,269 20,353 4,160
Rerata 4,047
1.
=
84 2. = 4,063% 3. = 4,160%
Rata-rata 4,047%
Hasil perhitungan kadar sari larut etanol
No Berat cawan + ekstrak setelah diuapkan Berat cawan kosong Berat ekstrak Kadar sari larut etanol (%)
1 64,698 64,623 5,078 1,48
2 78,469 78,400 5,045 1,37
3 72,133 72,062 5,058 1,40
Rerata 1,42
1. Kadar sari larut etanol =
= %
85 2. Kadar sari larut etanol =
=
= 1,37%
3. kadar sari larut etanol =
=
= 1,40%
86
LAMPIRAN D
HASIL PERHITUNGAN HARGA Rf
Hasil perhitungan harga Rf pada pemeriksaan secara KLT dengan pelarut n
-butanol : asam asetat : air (4 : 1 : 2)
Pengamatan Rf
UV 254 1 0,65
2
3
0,64
0,4
UV 366 1 0,65
2
3
0,64
0,4
Contoh perhitungan Rf
pada uv : 0,65
0,64
87
LAMPIRAN E
HASIL PERHITUNGAN LC50
Metode Probit analysis
Confidence Limits
Probabil
ity
95% Confidence Limits for
t_concentration
95% Confidence Limits for
log(t_concentration)b
Estimate
Lower
Bound
Upper
Bound Estimate
Lower
Bound
Upper
Bound
PROBITa .010 6.967 1.975 12.289 .843 .296 1.089
.020 8.521 2.756 14.295 .930 .440 1.155
.030 9.681 3.401 15.752 .986 .532 1.197
.040 10.658 3.980 16.957 1.028 .600 1.229
.050 11.524 4.522 18.015 1.062 .655 1.256
.060 12.317 5.038 18.975 1.090 .702 1.278
.070 13.056 5.537 19.867 1.116 .743 1.298
.080 13.756 6.023 20.708 1.139 .780 1.316
.090 14.425 6.501 21.510 1.159 .813 1.333
.100 15.070 6.972 22.282 1.178 .843 1.348
.150 18.060 9.280 25.874 1.257 .968 1.413
.200 20.854 11.589 29.288 1.319 1.064 1.467
.250 23.594 13.954 32.736 1.373 1.145 1.515
.300 26.359 16.402 36.360 1.421 1.215 1.561
.350 29.210 18.954 40.286 1.466 1.278 1.605
.400 32.200 21.622 44.646 1.508 1.335 1.650
.450 35.383 24.420 49.597 1.549 1.388 1.695
.500 38.824 27.366 55.329 1.589 1.437 1.743
88
.600 46.811 33.819 70.225 1.670 1.529 1.846
.650 51.602 37.432 80.215 1.713 1.573 1.904
.700 57.183 41.424 92.801 1.757 1.617 1.968
.750 63.886 45.962 109.201 1.805 1.662 2.038
.800 72.277 51.325 131.600 1.859 1.710 2.119
.850 83.459 58.050 164.478 1.921 1.764 2.216
.900 100.019 67.357 219.110 2.000 1.828 2.341
.910 104.489 69.765 235.013 2.019 1.844 2.371
.920 109.571 72.457 253.675 2.040 1.860 2.404
.930 115.445 75.512 275.999 2.062 1.878 2.441
.940 122.378 79.050 303.369 2.088 1.898 2.482
.950 130.795 83.252 338.051 2.117 1.920 2.529
.960 141.427 88.432 384.088 2.151 1.947 2.584
.970 155.688 95.184 449.648 2.192 1.979 2.653
.980 176.897 104.868 554.944 2.248 2.021 2.744
.990 216.343 121.966 774.462 2.335 2.086 2.889
a. A heterogeneity factor is used.
89
LAMPIRAN F
HASIL OUTPUT SPSS
Jam ke-1
Test of Homogeneity of Variances
d_temp
Levene Statistic df1 df2 Sig.
.000 4 20 1.000
ANOVA
d_temp
Sum of Squares df Mean Square F Sig.
Between Groups .430 4 .108 35.867 .000
Within Groups .060 20 .003
90
Post Hoc Tests
Multiple Comparisons
d_temp
Tukey HSD
(I) t_fr (J) t_fr
Mean
Difference
(I-J)
Std.
Error Sig.
95% Confidence Interval Lower Bound Upper Bound
t1 fr1 t1 fr2 -.08000 .03464 .183 -.1837 .0237
t1 fr3 -.28000* .03464 .000 -.3837 -.1763
t1 k(-) .12000* .03464 .018 .0163 .2237
t1 P -.10000 .03464 .062 -.2037 .0037
t1 fr2 t1 fr1 .08000 .03464 .183 -.0237 .1837
t1 fr3 -.20000* .03464 .000 -.3037 -.0963
t1 k(-) .20000* .03464 .000 .0963 .3037
t1 P -.02000 .03464 .977 -.1237 .0837
t1 fr3 t1 fr1 .28000* .03464 .000 .1763 .3837
t1 fr2 .20000* .03464 .000 .0963 .3037
t1 k(-) .40000* .03464 .000 .2963 .5037
t1 P .18000* .03464 .000 .0763 .2837
t1 k(-) t1 fr1 -.12000* .03464 .018 -.2237 -.0163
91
t1 fr3 -.40000* .03464 .000 -.5037 -.2963
t1 P -.22000* .03464 .000 -.3237 -.1163
t1 P t1 fr1 .10000 .03464 .062 -.0037 .2037
t1 fr2 .02000 .03464 .977 -.0837 .1237
t1 fr3 -.18000* .03464 .000 -.2837 -.0763
t1 k(-) .22000* .03464 .000 .1163 .3237
92 Jam ke-2
Test of Homogeneity of Variances
d_temp
Levene Statistic df1 df2 Sig.
.247 4 20 .908
ANOVA
d_temp
Sum of
Squares df
Mean
Square F Sig.
Between Groups .486 4 .121 24.280 .000
Within Groups .100 20 .005
93
Post Hoc Tests
Multiple Comparisons
d_temp
Tukey HSD
(I) t_fr (J) t_fr
Mean
Difference
(I-J)
Std.
Error Sig.
95% Confidence Interval Lower Bound Upper Bound
t2 fr1 t2 fr2 -.16000* .04472 .014 -.2938 -.0262
t2 fr3 -.26000* .04472 .000 -.3938 -.1262
t2 k(-) .14000* .04472 .038 .0062 .2738
t2 P -.14000* .04472 .038 -.2738 -.0062
t2 fr2 t2 fr1 .16000* .04472 .014 .0262 .2938
t2 fr3 -.10000 .04472 .207 -.2338 .0338
t2 k(-) .30000* .04472 .000 .1662 .4338
t2 P .02000 .04472 .991 -.1138 .1538
t2 fr3 t2 fr1 .26000* .04472 .000 .1262 .3938
t2 fr2 .10000 .04472 .207 -.0338 .2338
t2 k(-) .40000* .04472 .000 .2662 .5338
t2 P .12000 .04472 .092 -.0138 .2538
t2 k(-) t2 fr1 -.14000* .04472 .038 -.2738 -.0062
t2 fr2 -.30000* .04472 .000 -.4338 -.1662
94
t2 P -.28000* .04472 .000 -.4138 -.1462
t2 P t2 fr1 .14000* .04472 .038 .0062 .2738
t2 fr2 -.02000 .04472 .991 -.1538 .1138
t2 fr3 -.12000 .04472 .092 -.2538 .0138
t2 k(-) .28000* .04472 .000 .1462 .4138
95 Jam ke-3
Test of Homogeneity of Variances
d_temp
Levene Statistic df1 df2 Sig.
.603 4 20 .665
ANOVA
d_temp
Sum of
Squares df
Mean
Square F Sig.
Between Groups .886 4 .222 55.400 .000
Within Groups .080 20 .004
96
Post Hoc Tests
Multiple Comparisons
d_temp
Tukey HSD
(I) t_fr (J) t_fr
Mean
Difference
(I-J)
Std.
Error Sig.
95% Confidence Interval Lower Bound Upper Bound
t3 fr1 t3 fr2 -.16000* .04000 .006 -.2797 -.0403
t3 fr3 -.28000* .04000 .000 -.3997 -.1603
t3 k(-) .16000* .04000 .006 .0403 .2797
t3 P -.36000* .04000 .000 -.4797 -.2403
t3 fr2 t3 fr1 .16000* .04000 .006 .0403 .2797
t3 fr3 -.12000* .04000 .049 -.2397 -.0003
t3 k(-) .32000* .04000 .000 .2003 .4397
t3 P -.20000* .04000 .001 -.3197 -.0803
t3 fr3 t3 fr1 .28000* .04000 .000 .1603 .3997
t3 fr2 .12000* .04000 .049 .0003 .2397
t3 k(-) .44000* .04000 .000 .3203 .5597
t3 P -.08000 .04000 .302 -.1997 .0397
t3 k(-) t3 fr1 -.16000* .04000 .006 -.2797 -.0403
97
t3 fr3 -.44000* .04000 .000 -.5597 -.3203
t3 P -.52000* .04000 .000 -.6397 -.4003
t3 P t3 fr1 .36000* .04000 .000 .2403 .4797
t3 fr2 .20000* .04000 .001 .0803 .3197
t3 fr3 .08000 .04000 .302 -.0397 .1997
t3 k(-) .52000* .04000 .000 .4003 .6397
98 Jam ke-4
Test of Homogeneity of Variances
d_temp
Levene Statistic df1 df2 Sig.
.564 4 20 .692
ANOVA
d_temp
Sum of
Squares df
Mean
Square F Sig.
Between Groups 1.990 4 .497 67.216 .000
Within Groups .148 20 .007
99
Post Hoc Tests
Multiple Comparisons
d_temp
Tukey HSD
(I) t_fr (J) t_fr
Mean
Difference
(I-J)
Std.
Error Sig.
95% Confidence Interval Lower Bound Upper Bound
t4 fr1 t4 fr2 -.10000 .05441 .381 -.2628 .0628
t4 fr3 -.46000* .05441 .000 -.6228 -.2972
t4 k(-) .26000* .05441 .001 .0972 .4228
t4 P -.48000* .05441 .000 -.6428 -.3172
t4 fr2 t4 fr1 .10000 .05441 .381 -.0628 .2628
t4 fr3 -.36000* .05441 .000 -.5228 -.1972
t4 k(-) .36000* .05441 .000 .1972 .5228
t4 P -.38000* .05441 .000 -.5428 -.2172
t4 fr3 t4 fr1 .46000* .05441 .000 .2972 .6228
t4 fr2 .36000* .05441 .000 .1972 .5228
t4 k(-) .72000* .05441 .000 .5572 .8828
t4 P -.02000 .05441 .996 -.1828 .1428
100
t4 fr2 -.36000* .05441 .000 -.5228 -.1972
t4 fr3 -.72000* .05441 .000 -.8828 -.5572
t4 P -.74000* .05441 .000 -.9028 -.5772
t4 P t4 fr1 .48000* .05441 .000 .3172 .6428
t4 fr2 .38000* .05441 .000 .2172 .5428
t4 fr3 .02000 .05441 .996 -.1428 .1828
t4 k(-) .74000* .05441 .000 .5772 .9028
101
LAMPIRAN G
102
103
LAMPIRAN H
