Lampiran 1. Hasil identifikasi tanaman
Lampiran 2. Karakteristik daun pugun tanoh
Makroskopik daun pugun tanoh
Serbuk simplisia daun pugun tanoh
c d e
f
g h
Mikroskopik serbuk simplisia daun pugun tanoh
Keterangan:
a. Sel epidermis b. tulang daun c. Trikoma
d. Kristal kalsium oksalat bentuk prisma e. Berkas pembuluh angkut bentuk spiral f. Stomata tipe diasitik
g. Stomata tipe anomositik h. Sel tetangga
a
b
Lampiran 3. Hasil karakterisasi Simplisia
1. Penetapan Kadar air
% Kadar air simplisia = volume air (ml )
berat sampel (g) x 100%
No. Berat sampel (g) Volume awal (ml) Volume akhir (ml)
1. 5,043 1,80 2,10
2. 5,032 2,10 2,40
3. 5,021 2,40 2,70
% Kadar air = volume akhir−volume awal
berat sampel x 100%
1. % Kadar air = 2,10− 1,80
5,043 x 100% = 5,95%
2. % Kadar air = 2,40− 2,10
5,032 x 100% = 5,96%
3. % Kadar air = 2,70− 2,40
5,021 x 100% = 5,97%
% rata-rata kadar air = 5,95% + 5,96% +5,97%
3 = 5,96%
2. Penetapan Kadar Sari Larut Air
3. Penetapan Kadar Sari Larut Etanol
% Kadar sari larut dalam etanol = berat sari (g)
4. Penetapan Kadar Abu Total
5. Penetapan Kadar Abu Tidak Larut Asam
% Kadar abu total = berat abu (g)
% rata-rata kadar abu tidak larut asam = 1,04%+1,03%+0,94%
3 = 1,00%
Lampiran 4. Alat organ bath
Bejana organ
Monitor komputer dan seperangkat alat Power Lab
Lampiran 5. Pola kenaikan kontraksi otot polos trakea marmut terisolasi setelah diinduksi asetilkolin konsentrasi bertingkat
Lampiran 6. Pola penurunan kontraksi otot polos trakea marmut terisolasi setelah pemberian EEDPT
Lampiran 7. Perhitungan effective concentration (EC80) asetilkolin terhadap otot polos trakea
Log
konsentrasi Ach
% Kontraksi Trakea
Mean SEM -2.52 100.0000 100.0000 100.0000 100.0000 100.0000 0.0000
Log EC80=�
3. Log EC80=� 80 –66,2069
100 – 66,2069 × (−2.5−(−3))�+ −3
= -2,8041
EC80 = 1,57x10-3 M
4. Log EC80=� 80 –66,6667
100 – 66,6667 × (−2.5−(−3))�+ −3
= -2,808
EC80 = 1,56x10-3 M
EC80 rata-rata =
1,05+1,40+1,57+1,56
4
x
10-3
M = 1,39x10-3 M
Lampiran 8. Analisis uji-t independen nilai % relaksasi EEDPT terhadap kontraksi otot polos trakea oleh pemberian asetilkolin 10-3 M dengan inkubasi awal menggunakan teofilin 10-4 M selama 20 menit dengan % relaksasi EEDPT terhadap kontraksi otot polos trakea oleh pemberian asetilkolin 10-3 M tanpa inkubasi sebagai kontrol
Tests of Normality
Kolmogorov-Smirnov(a) Shapiro-Wilk
Statistic df Sig. Statistic df Sig.
* This is a lower bound of the true significance. a Lilliefors Significance Correction
T-Test
Group Statistics
perlakuan N Mean
Independent Samples Test
.266 .620 .211 8 .838 .4321800 2.05158 -4.29876 5.16312
.211 7.584 .839 .4321800 2.05158 -4.34435 5.20871
1.337 .281 .121 8 .907 .6057200 5.00399 -10.9335 12.1450
.121 5.983 .908 .6057200 5.00399 -11.6470 12.8584
.308 .594 -.305 8 .768 -1.610940 5.27321 -13.7710 10.5491
-.305 7.690 .768 -1.610940 5.27321 -13.8569 10.6350
.017 .901 -1.231 8 .253 -9.080700 7.37844 -26.0954 7.93401
-1.231 7.995 .253 -9.080700 7.37844 -26.0972 7.93577
1.163 .312 -2.005 8 .080 -15.42974 7.69640 -33.1777 2.31820
-2.005 7.678 .081 -15.42974 7.69640 -33.3081 2.44867
.768 .406 -1.927 8 .090 -19.30150 10.0165 -42.3997 3.79668
-1.927 6.466 .099 -19.30150 10.0165 -43.3896 4.78656
.815 .393 -1.851 8 .101 -12.63156 6.82256 -28.3644 3.10129
-1.851 5.302 .120 -12.63156 6.82256 -29.8732 4.61011
1.172 .311 -.710 8 .498 -6.833180 9.62173 -29.0209 15.3546
-.710 5.985 .504 -6.833180 9.62173 -30.3907 16.7243 Equal for Equality of Variances
e Lower Upper 95% Confidence
Interval of the Difference t-test for Equality of Means
Lampiran 9. Uji korelasi regresi antara dosis EEDPT dengan besarnya efek relaksasi otot polos trakea
Correlations
Regression
De scri ptive Statistics
4.50 2.449 8
53.799963 40.3856117 8
dosis E EP T % relaksas i
Mean St d. Deviat ion N
% relaksas i
dosis EEPT % relaksas i
Correlation is s ignificant at the 0.01 level (2-tailed). **.
De scri ptive Statistics
53.799963 40.3856117 8
4.50 2.449 8
% relaksas i dosis E EP T
Va riables Entere d/Re movedb
All reques ted variables ent ered. a.
Dependent Variable: % relaksasi b.
Model Summary
.981a .963 .957 8.4039254
Model 1
R R Square
Adjusted R Square
Std. Error of the Estimate
Predictors: (Constant), dosis EEPT a.
Coeffi cientsa
-19.003 6.548 -2. 902 .027
16.178 1.297 .981 12.476 .000
(Const ant) St andardiz ed
Coeffic ients
t Sig.
Dependent Variable: % relak sas i a.
