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CHAPTER 3
THEORITICAL FRAMEWORK, CONCEPTUAL FRAMEWORK AND HYPOTHESIS
3.1Theoretical Framework
Figure 2. Theoretical Framework of the study Hepatotoxic metabolites
(bromide and fluoride ions, other volatil metabolites)
Liver cells damage (nucleus changes: enlarge,
karyorhexis, karyolysis)
Altered liver antigens Specific
antibody
Liver cells necrosis Moderate / Severe Liver cells
necrosis
Liver protein Increasing
Trifluroacetyl-acid production Induction of
Cytochrom P450 (2A6 and 3A4)
Genotypic and phenotypic variability
in metabolism, cytoprotection and
immune response Halothane
27 3.2 Conceptual Framework
Independent Variable Dependent Variable
Figure 3. Conceptual Framework of the study
3.3 Hypothesis
3.3.1 There is an association between the time of halothane exposure with severity of liver cells nucleus changes
3.3.2 Halothane exposure can induce changes of cytochrome P450 in the liver
Cytochrome P450 2E1 changes
Halothane
