KAJIAN IN VITRO PENGGUNAAN KROMIUM ORGANIK DALAM RANSUM SAPI PERAH TERHADAP POPULASI MIKROBA RUMEN
DAN PRODUKSI GAS TOTAL
Panji Akbar Wijayadikusumah
ABSTRAK
Penelitian dilaksanakan selama satu bulan, dari bulan Februari sampai Maret 2013 di Laboratorium Nutrisi Ternak Ruminansia dan Kimia Makanan Ternak dan di Laboratorium Mikrobiologi dan Penanganan Limbah Peternakan, Fakultas Peternakan, Universitas Padjadjaran. Penelitian bertujuan untuk mengetahui pengaruh penggunaan kromium organik dalam ransum sapi perah terhadap populasi mikroba rumen dan produksi gas total yang dikaji secara in vitro. Penelitian dilakukan dengan metode eksperimental, rancangan percobaan yang digunakan adalah Rancangan Acak Lengkap (RAL) dengan empat perlakuan penggunaan kromium organik, yaitu : R1= 0 ppm, R2= 1,5 ppm, R3 = 3 ppm dan R4 = 4,5 ppm, masing-masing perlakuan diulang sebanyak lima kali. Data yang diperoleh dianalisis dengan menggunakan Analisis Sidik Ragam, kemudian untuk mengetahui pebedaan antar perlakuan dilakukan uji lanjut menggunakan, Uji Jarak Berganda Duncan. Peubah yang diukur adalah jumlah populasi mikroba rumen dan produksi gas total. Hasil penelitian menunjukkan bahwa penggunaan kromium organik dalam ransum sapi perah berpengaruh terhadap jumlah total populasi bakteri dan produksi gas total, tetapi tidak berpengaruh terhadap jumlah populasi protozoa. Penggunaan 4,5 ppm kromium organik dalam ransum menghasilkan nilai rataan total populasi bakteri, populasi protozoa dan produksi gas tertinggi.
IN VITRO STUDY OF ORGANIC CHROMIUM IN DAIRY CATTLE RATION ON RUMEN MICROBES POPULATION AND TOTAL GAS
PRODUCTION
Panji Akbar Wijayadikusumah
ABSTRACT
This research was conducted for one month, from February to March 2013 at Laboratory of Animal Nutrition of Ruminant and Feed Chemistry and at Laboratory Microbiology and Animal Husbandry Waste Management, Faculty of Animal Husbandry, Padjadjaran University. The study aimed to determine the effect of the use of organic chromium in the ration of Dairy Cattle on rumen microbial population and total gas production which was studied in In Vitro. Research was carried out by the experimental method, experimental design used was completely randomized design (CRD) with four treatments using organic chromium, namely: R1 = 0 ppm, R2 = 1.5 ppm, R3 = R4 = 3 ppm and 4.5 ppm and each treatment was repeated five times. The data obtained were analyzed by using the Fingerprint Analysis of Variety and then to determine the average difference among treatments, it was done the tested further with Duncan Multiple Range Test. The variables measured were the number of rumen microbial population and total gas production. The results were showed that the use of organic chromium in dairy cow rations affected the total bacterial population and total gas production, but had no effect on protozoa population. The use of 4.5 ppm chromium in the ration of organic produced average value of the total population of bacteria, protozoa population and the highest gas production.
