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Lampiran 1 Bahan-bahan untuk analisis kandungan IAA dan gula total
Peubah yang diamati Bahan yang digunakan a. Analisis kandungan IAA
b. Analisis kandungan gula total
1. Larutan ekstraksi (60 ML): 36 ml methanol 15 ml kloroform 9 ml NH4OH 2N 2. HCl 1N 3. NaOH 1N
4. Reagen Salkowski mengandung: 150 ml H2SO4 250 ml Aquades 7.5 ml FeCl36H2O 0.5 M (Aryantha et al. 2004). 5. Hormon IAA pa 5 mg 1. Reagen Anthrone 0.1%: 1000 ml H2SO4 1 gr Anthrone 2. Etanol 80%
Lampiran 2 Prosedur analisis kandungan IAA
Spektrofotometer λ 510 nm 1 ml sample + 4 ml salkowsky
Evaporasi dan inkubasi 1 jam pada suhu ruang Ekstraksi dengan etil asetat 15 ml 3 x
Fase atas + HCl 1N hingga pH2.5 Evaporasi (uapkan methanol)
Buang fase atas (kloroform)
Tambahkan larutan ekstraksi dan 25 ml aquades 1 g sampel buah digerus
Lampiran 3 Prosedur analisis kandungan gula total
25 ml etanol 80% (panas), kocok 5 menit Residu
25 ml etanol 80% (panas), kocok 5 menit
Sentrifugasi 4000 rpm 15 menit Sentrifugasi 4000 rpm 15 menit Supernatan II Spektrofotometer λ 630 nm Esbath Waterbath 100°C, 12 menit 1 ml sampel,1 ml H2O, 5 ml Anthrone
Tera 50 ml Uapkan 85°C
Supernatan I 200 mg sampel kering halus + 2 tetes etanol 80% + 5 ml H2O
Lampiran 4 Sidik ragam pengaruh GA3 dan 2,4-D terhadap kerontokan bunga dan kerontokan buah
Sidik ragam pengaruh GA3 dan 2,4-D terhadap kerontokan bunga Sumber keragaman DB JK KT F Hitung F Tabel 5% P 2,4-D 3 0.325478 0.108493 86.61 2.90 0.000 GA3 3 0.043606 0.014535 11.60 2.90 0.000 Kombinasi 15 0.82063 0.05471 11.54 2.02 0.000 Interaksi 9 0.563172 0.062575 49.95 2.19 0.000 Galat 32 0.040086 0.001253 Total 47 0.972342 R-Sq = 95.88%
Sidik ragam pengaruh GA3 dan 2,4-D terhadap kerontokan buah Sumber
keragaman DB JK KT F Hitung F Tabel 5% P
2,4-D 3 0.139205 0.0464018 26.06 2.90 0.000 GA3 3 0.031895 0.0106317 5.97 2.90 0.002 Kombinasi 15 0.47580 0.03172 17.81 2.02 0.000 Interaksi 9 0.304704 0.0338560 19.01 2.19 0.000 Galat 32 0.056981 0.0017806 Total 47 0.532785 R-Sq = 89.31%
Lampiran 5 Sidik ragam pengaruh GA3 dan 2,4-D terhadap jumlah buah per-tandan Sumber keragaman DB JK KT F Hitung F Tabel 5% P 2,4-D 3 4.4084 1.46947 20.71 2.90 0.000 GA3 3 0.7151 0.23836 3.36 2.90 0.031 Kombinasi 15 13.6105 0.9074 12.79 2.02 0.000 Interaksi 9 8.4871 0.94301 13.29 2.19 0.000 Galat 32 2.2705 0.07095 Total 47 15.8810 R-Sq = 85.70%
Lampiran 6 Sidik ragam pengaruh GA3 dan 2,4-D terhadap kandungan IAA buah
Sumber
keragaman DB JK KT F Hitung F Tabel 5% P 2,4-D 3 57361.1 19120.4 68.03 3.24 0.000 GA3 3 26897.4 8965.8 31.90 3.24 0.000 Kombinasi 15 84487 5632 20.04 2.33 0.000 Interaksi 9 228.5 25.4 0.09 2.54 1.000 Galat 32 4496.8 281.0 Total 47 88983.7 R-Sq = 94.95%
Lampiran 7 Sidik ragam pengaruh GA3 dan 2,4-D terhadap kandungan gula total daun setelah Perlakuan I dan setelah Perlakuan II
Sidik ragam pengaruh GA3 dan 2,4-D terhadap kandungan gula total daun setelah Perlakuan I
Sumber
keragaman DB JK KT F Hitung F Tabel 5% P 2,4-D 3 30.334 10.1113 16.21 3.24 0.000 GA3 3 8.512 2.8374 4.55 3.24 0.017 Kombinasi 15 108.169 7.211 11.56 2.33 0.000 Interaksi 9 69.323 7.7026 12.35 2.54 0.000 Galat 32 9.978 0.6236 Total 47 118.147 R-Sq = 91.55%
Sidik ragam pengaruh GA3 dan 2,4-D terhadap kandungan gula total daun setelah Perlakuan II Sumber keragaman DB JK KT F Hitung F Tabel 5% P 2,4-D 3 35.889 11.9629 14.97 3.24 0.000 GA3 3 13.415 4.4717 5.59 3.24 0.008 Kombinasi 15 150.996 10.066 12.59 2.33 0.000 Interaksi 9 101.692 11.2991 14.14 2.54 0.000 Galat 32 12.790 0.7994 Total 47 163.785 R-Sq = 92.19%
Lampiran 8 Sidik ragam pengaruh GA3 dan 2,4-D terhadap bobot per-buah dan bobot buah per-tandan
Sidik ragam pengaruh GA3 dan 2,4-D terhadap bobot per-buah Sumber keragaman DB JK KT F Hitung F Tabel 5% P 2,4-D 3 3961.39 1320.46 81.23 2.90 0.000 GA3 3 512.95 170.98 10.52 2.90 0.000 Kombinasi 15 4308.1 287.2 13.10 2.02 0.000 Interaksi 9 15.27 1.70 0.10 2.19 0.999 Galat 32 520.17 16.26 Total 47 R-Sq = 89.62%
Sidik ragam pengaruh GA3 dan 2,4-D terhadap bobot buah pe- tandan Sumber keragaman DB JK KT F Hitung F Tabel 5% P 2,4-D 3 348103 116034 32.18 2.90 0.000 GA3 3 49778 16593 4.60 2.90 0.009 Kombinasi 15 819551 54637 15.15 2.02 0.000 Interaksi 9 421670 46852 12.99 2.19 0.000 Galat 32 115402 3606 Total 47 934954 R-Sq = 87.66%
Lampiran 9 Tabel Analisis ragam regresi pengaruh kandungan gula total daun terhadap persentase kerontokan bunga dan persentase kerontokan buah
Tabel Analisis ragam regresi pengaruh kandungan gula total daun terhadap persentase kerontokan bunga
Model DB JK KT F Hitung P Regression 1 0.287 0.287 158.181 0 Residual 14 0.025 0.002 Total 15 0.312 R-sq= 0.919
Tabel Analisis ragam regresi pengaruh kandungan gula total daun terhadap persentase kerontokan buah
Model DB JK KT F Hitung P Regression 1 0.145 0.45 146.044 0 Residual 14 0.014 0.001 Total 15 0.159 R-sq= 0.913
