Penampang melintang tubulus seminiferus testis setelah pemberian vitamin C
dan E pada mencit yang dipajankan monosodium glutamat (MSG), pewarnaan
HE, Perbesaran 400x, (Konversi 20:1).
Ditimbang sebanyak 100 mg
Dimasukkan ke dalam beaker glass
Ditambahkan aquadest sebanyak 0,2 ml
Diaduk dengan menggunakan spatula
Ditimbang sebanyak 6,5 mg
Dimasukkan ke dalam beaker glass
Ditambahkan aquadest sebanyak 0,2 ml
Diaduk dengan menggunakan spatula
Diambil sebanyak0,65 mg
Dilarutkan dengan minyak jarak “Oleum Riccini” 0,3 ml
Serbuk MSG
Larutan MSG
Serbuk Vitamin C
Larutan Vitamin C
Vitamin E
Dibilas dengan NaCl 0,9%
Difiksasi dalam BOUIN selama 1 malam
Washing
dalam alkohol 70%
Dehidrasi dengan alkohol konsentrasi bertingkat mulai 70%,
80%, 96%, dan 100%
Clearing
dalam xylol
Infiltrasi
Embedding
(penanaman) organ dalam cetakan kemudian
dituangkan parafin murni, dibiarkan hingga didapatkan blok
parafin
Cutting
(pemotongan) menggunakan mikrotom sehingga
didapatkan pita-pita parafin
Attaching
(penempelan) pita parafin pada objek glass
Deparafinasi dengan mencelupkan objek dalam xylol
Dealkoholisasi dalam alkohol menurun dari 100%, 96%, 80%,
hingga 70%
Pewarnaan dengan mencelupkan dalam Hematoxilin selama 3-7
menit kemudian dibilas dengan air mengalir. Selanjutnya
dicelupkan dalam alkohol 70%, lalu dicelupkan dalam Eosin
selama 1-3 menit.
Mounting
yaitu menutup preparat dengan gelas penutup yang
sebelumnya diberi Canada balsam
Vitamin E
Castrol Oil
Kandang Mencit
Jarum Gavage
Rataan Berat Testis setelah Pemberian Vitamin C dan E pada mencit (
Mus
musculus
L.) yang Dipajankan Monosodium Glutamat (MSG)
Perlakuan
Ulangan
x
± SD
Hasil Uji Statistik Berat Testis Mencit
Tests of Normality
kelomp
ok
Kolmogorov-Smirnova Shapiro-Wilk
Statistic df Sig. Statistic df Sig.
a. Lilliefors Significance Correction
*. This is a lower bound of the true significance.
Test of Homogeneity of Variance
Levene Statistic df1 df2 Sig.
berat_testis Based on Mean 2.486 5 24 .060
Based on Median .898 5 24 .498
Based on Median and with
adjusted df
.898 5 16.777 .505
ok N Mean Rank
Test Statisticsa,b
berat_testis
Chi-Square 13.449
df 5
Asymp. Sig. .020
a. Kruskal Wallis Test
b. Grouping Variable:
Wilcoxon W 26.000
Z -.346
Asymp. Sig. (2-tailed) .729
Exact Sig. [2*(1-tailed Sig.)] .841a
a. Not corrected for ties.
b. Grouping Variable: kelompok
Ranks
Test Statisticsb
berat_testis
Mann-Whitney U .000
Wilcoxon W 15.000
Z -2.668
Asymp. Sig. (2-tailed) .008
Exact Sig. [2*(1-tailed Sig.)] .008a
a. Not corrected for ties.
b. Grouping Variable: kelompok
Wilcoxon W 27.000
Z -.113
Asymp. Sig. (2-tailed) .910
Exact Sig. [2*(1-tailed Sig.)] 1.000a
a. Not corrected for ties.
b. Grouping Variable: kelompok
Ranks
Test Statisticsb
berat_testis
Mann-Whitney U 8.500
Wilcoxon W 23.500
Z -.956
Asymp. Sig. (2-tailed) .339
Exact Sig. [2*(1-tailed Sig.)] .421a
a. Not corrected for ties.
b. Grouping Variable: kelompok
Ranks
kelomp
ok N Mean Rank Sum of Ranks
berat_testis k- 5 5.10 25.50
Wilcoxon W 25.500
Z -.438
Asymp. Sig. (2-tailed) .661
Exact Sig. [2*(1-tailed Sig.)] .690a
a. Not corrected for ties.
b. Grouping Variable: kelompok
Ranks
Test Statisticsb
berat_testis
Mann-Whitney U .000
Wilcoxon W 15.000
Z -2.643
Asymp. Sig. (2-tailed) .008
Exact Sig. [2*(1-tailed Sig.)] .008a
a. Not corrected for ties.
b. Grouping Variable: kelompok
Wilcoxon W 26.500
Z -.219
Asymp. Sig. (2-tailed) .827
Exact Sig. [2*(1-tailed Sig.)] .841a
a. Not corrected for ties.
b. Grouping Variable: kelompok
Ranks
Test Statisticsb
berat_testis
Mann-Whitney U 10.500
Wilcoxon W 25.500
Z -.454
Asymp. Sig. (2-tailed) .650
Exact Sig. [2*(1-tailed Sig.)] .690a
a. Not corrected for ties.
b. Grouping Variable: kelompok
Ranks
kelomp
ok N Mean Rank Sum of Ranks
berat_testis k+ 5 5.40 27.00
Wilcoxon W 27.000
Z -.108
Asymp. Sig. (2-tailed) .914
Exact Sig. [2*(1-tailed Sig.)] 1.000a
a. Not corrected for ties.
b. Grouping Variable: kelompok
Ranks
Test Statisticsb
berat_testis
Mann-Whitney U .000
Wilcoxon W 15.000
Z -2.635
Asymp. Sig. (2-tailed) .008
Exact Sig. [2*(1-tailed Sig.)] .008a
a. Not corrected for ties.
b. Grouping Variable: kelompok
Wilcoxon W 15.000
Z -2.660
Asymp. Sig. (2-tailed) .008
Exact Sig. [2*(1-tailed Sig.)] .008a
a. Not corrected for ties.
b. Grouping Variable: kelompok
Ranks
Test Statisticsb
berat_testis
Mann-Whitney U .000
Wilcoxon W 15.000
Z -2.635
Asymp. Sig. (2-tailed) .008
Exact Sig. [2*(1-tailed Sig.)] .008a
a. Not corrected for ties.
b. Grouping Variable: kelompok
Ranks
kelomp
ok N Mean Rank Sum of Ranks
berat_testis p2 5 5.00 25.00
Wilcoxon W 25.000
Z -.561
Asymp. Sig. (2-tailed) .575
Exact Sig. [2*(1-tailed Sig.)] .690a
a. Not corrected for ties.
b. Grouping Variable: kelompok
Ranks
Test Statisticsb
berat_testis
Mann-Whitney U 11.000
Wilcoxon W 26.000
Z -.323
Asymp. Sig. (2-tailed) .746
Exact Sig. [2*(1-tailed Sig.)] .841a
a. Not corrected for ties.
b. Grouping Variable: kelompok
Wilcoxon W 27.000
Z -.110
Asymp. Sig. (2-tailed) .913
Exact Sig. [2*(1-tailed Sig.)] 1.000a
a. Not corrected for ties.
Rataan Volume Testis setelah Pemberian Vitamin C dan E pada Mencit (
Mus
musculus
L.) yang Dipajankan Monosodium Glutamat (MSG)
Perlakuan
Ulangan
x
± SD
Hasil Uji Statistik Volume Testis Mencit
Tests of Normality
KELOM
POK
Kolmogorov-Smirnova Shapiro-Wilk
Statistic df Sig. Statistic df Sig.
a. Lilliefors Significance Correction
*. This is a lower bound of the true significance.
Test of Homogeneity of Variance
Levene Statistic df1 df2 Sig.
VOLUME_TESTIS Based on Mean .569 5 24 .723
Based on Median .300 5 24 .908
Based on Median and with
adjusted df
.300 5 18.887 .907
Sum of Squares df Mean Square F Sig.
Between Groups 5721.774 5 1144.355 4.545 .005
Within Groups 6042.218 24 251.759
Total 11763.992 29
Mean Difference
(I-J) Std. Error Sig.
95% Confidence Interval
Lower Bound Upper Bound
P1 37.59400* 10.03512 .015 4.8957 70.2923
P2 14.89400 10.03512 1.000 -17.8043 47.5923
P4 1.01400 10.03512 1.000 -31.6843 33.7123
P4 K- -.52600 10.03512 1.000 -33.2243 32.1723
K+ -.66200 10.03512 1.000 -33.3603 32.0363
P1 36.58000* 10.03512 .019 3.8817 69.2783
P2 13.88000 10.03512 1.000 -18.8183 46.5783
P3 -1.01400 10.03512 1.000 -33.7123 31.6843
Rataan Diameter Tubulus Seminiferus Testis setelah Pemberian Vitamin C dan
E pada Mencit (
Mus musculus
L.) yang Dipajankan Monosodium Glutamat
(MSG)
Hasil Uji Statistik Diameter Tubulus Seminiferus Testis Mencit
Tests of Normality
KELOM
POK
Kolmogorov-Smirnova Shapiro-Wilk
Statistic df Sig. Statistic df Sig.
a. Lilliefors Significance Correction
*. This is a lower bound of the true significance.
Test of Homogeneity of Variance
Levene Statistic df1 df2 Sig.
ANOVA
DIAMETER
Sum of Squares df Mean Square F Sig.
Between Groups 2191495.311 5 438299.062 3.505 .016
Within Groups 3000952.077 24 125039.670
Total 5192447.388 29
Mean Difference
(I-J) Std. Error Sig.
95% Confidence Interval
Lower Bound Upper Bound
K- K+ -29.42200 223.64228 1.000 -758.1339 699.2899
P1 738.38800* 223.64228 .045 9.6761 1467.0999
P2 304.28200 223.64228 1.000 -424.4299 1032.9939
P3 147.31200 223.64228 1.000 -581.3999 876.0239
P4 -6.15200 223.64228 1.000 -734.8639 722.5599
K+ K- 29.42200 223.64228 1.000 -699.2899 758.1339
P1 767.81000* 223.64228 .033 39.0981 1496.5219
P2 333.70400 223.64228 1.000 -395.0079 1062.4159
P3 176.73400 223.64228 1.000 -551.9779 905.4459
P4 23.27000 223.64228 1.000 -705.4419 751.9819
P1 K- -738.38800* 223.64228 .045 -1467.0999 -9.6761
K+ -767.81000* 223.64228 .033 -1496.5219 -39.0981
P2 -434.10600 223.64228 .961 -1162.8179 294.6059
P3 -591.07600 223.64228 .214 -1319.7879 137.6359
P4 -744.54000* 223.64228 .042 -1473.2519 -15.8281
P2 K- -304.28200 223.64228 1.000 -1032.9939 424.4299
P3 K- -147.31200 223.64228 1.000 -876.0239 581.3999
K+ -176.73400 223.64228 1.000 -905.4459 551.9779
P1 591.07600 223.64228 .214 -137.6359 1319.7879
P2 156.97000 223.64228 1.000 -571.7419 885.6819
P4 -153.46400 223.64228 1.000 -882.1759 575.2479
P4 K- 6.15200 223.64228 1.000 -722.5599 734.8639
K+ -23.27000 223.64228 1.000 -751.9819 705.4419
P1 744.54000* 223.64228 .042 15.8281 1473.2519
P2 310.43400 223.64228 1.000 -418.2779 1039.1459
P3 153.46400 223.64228 1.000 -575.2479 882.1759
Sel yang
Hasil Uji Statistik Jumlah Sel Spermatogenik Testis Mencit
Tests of Normality
kelomp
ok
Kolmogorov-Smirnova Shapiro-Wilk
Statistic df Sig. Statistic df Sig.
a. Lilliefors Significance Correction
spermatogonium Based on Mean .589 5 24 .708
Based on Median .407 5 24 .839
Based on Median and with
adjusted df
.407 5 19.977 .838
Mean Difference
(I-J) Std. Error Sig.
95% Confidence Interval
Lower Bound Upper Bound
pok Statistic df Sig. Statistic df Sig.
a. Lilliefors Significance Correction
*. This is a lower bound of the true significance.
Test of Homogeneity of Variance
Levene
Statistic df1 df2 Sig.
spermatosit_primer Based on Mean 1.008 5 24 .434
Based on Median .854 5 24 .526
Based on Median and
with adjusted df
(I)
Mean Difference
(I-J) Std. Error Sig.
95% Confidence Interval
Lower Bound Upper Bound
Tests of Normality
kelomp
ok
Kolmogorov-Smirnova Shapiro-Wilk
Statistic df Sig. Statistic df Sig.
a. Lilliefors Significance Correction
*. This is a lower bound of the true significance.
Test of Homogeneity of Variance
Levene Statistic df1 df2 Sig.
spermatid Based on Mean 2.168 5 24 .092
Based on Median 1.578 5 24 .204
Based on Median and with
adjusted df
(I)
Mean Difference
(I-J) Std. Error Sig.
95% Confidence Interval
Lower Bound Upper Bound
