Laporan Ir

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LAPORAN PRAKTIKUM ANALISIS SEDIAAN FARMASI LAPORAN PRAKTIKUM ANALISIS SEDIAAN FARMASI

ANALISIS SENYAWA KAFEIN ANALISIS SENYAWA KAFEIN

DENGAN METODE SPEKTROFOTOMETRI FTIR DENGAN METODE SPEKTROFOTOMETRI FTIR

Oleh : Oleh :

Golongan Q / Kelompo D Golongan Q / Kelompo D !

!"" LL##$$%%##nna a AA" " YYooee &&''''(())!!((&&''!! &

&"" MMaa**##a a ++" " RR" " RRaa$$,,aa &&''''(())!!((&&''(( (

("" ++##nn# # SS" " TTaannaaeemm &&''''(())!!((&&--.. '

'"" 00aan n SS" " GGeenn$$**aa &'&'''(())!!((&&11((

A2#23en P*a3#0m: 4en*5 K" S" A2#23en P*a3#0m: 4en*5 K" S"

FAKULT

FAKULTAS AS FARMASIFARMASI

UNI+ERSITAS KATOLIK WIDYA MANDALA SURA6AYA UNI+ERSITAS KATOLIK WIDYA MANDALA SURA6AYA

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&)!-I. Tujuan Praktikum

• Un30 mempela,a*# 7aga#mana 8a*a meng#$en3#9#a2# 7ahan 7a0 o7a3 / 2en5a%a

ha2#l 2#n3e2#2 $engan mengg0naan 2pe3*o9o3ome3e* FTIR

• Un30 mela0an #n3e*p*e3a2# $a3a 2pe3*0m IR $a*# a3 5ang $#anal#2a"

II. Dasar Teori

Spe3*o2op# #n9*ame*ah me*0paan 2alah 2a30 ala3 5ang 7an5a $#paa# 0n30 meng#$en3#9#a2# 2en5a%a; 7a# alam# ma0p0n 70a3an" Dalam 7#$ang 9#2#a 7ahan; 2epe*3# 7ahan<7ahan pol#me*; #n9*ame*ah ,0ga $#paa# 0n30 menga*a3e*#2a2# 2ampel" S0a30 en$ala 5ang men50l#3an $alam meng#$en3#9#a2# 2en5a%a $engan #n9*ame*ah a$alah 3#$a a$an5a a30*an 5ang 7a0 0n30 mela0an #n3e*p*e3a2# 2pe3*0m" Ka*ena omple2n5a #n3e*a2# $alam =#7*a2# mole0l $alam 20a30 2en5a%a $an e9e<e9e e23e*nal 5ang 20l#3 $#on3*ol 2e*#ngal# p*e$#2# 3eo*e3# 3#$a lag# 2e20a#" Penge3ah0an $alam hal #n# 2e7ag#an 7e2a* $#pe*oleh 2e8a*a emp#*#2 $an pengalaman >6a22e3; !??'@"

Pa$a $a2a*n5a Spe3*o9o3ome3e* FTIR >Fo0*#e* T*a29o*m In9*a Re$@ a$alah 2ama $engan Spe3*o9o3ome3e* IR $#2pe*2#; 5ang mem7e$aann5a a$alah pengem7angan pa$a 2#23#m op3#n5a 2e7el0m 7e*a2 2#na* #n9*a me*ah mele%a3# 8on3oh" Da2a* pem##*an $a*# Spe3*o9o3ome3e* FTIR a$alah $a*# pe*2amaan gelom7ang 5ang $#*0m02an oleh ean 6ap3#23e o2eph Fo0*#e* >!1.<!()@ 2eo*ang ahl# ma3ema3#a $a*# Pe*an8#2" Fo0*#e* mengem0aan $e*e3 pe*2amaan gelom7ang ele3*on# 2e7aga# :

• a $an 7 me*0paan 20a30 3e3apan • 3 a$alah %a30

• B a$alah 9*e0en2# 20$03 >*a$#an pe* $e3#@

> B C &  9 $an 9 a$alah 9*e0en2# $alam 4e*3@ >G#%anga*a;&)).@

Me3o$e Spe3*o2op# #n9*ame*ah #n# $apa3 $#g0naan 0n30 meng#$en3#9#a2# 20a30 2en5a%a 5ang 7el0m $#e3ah0#;a*ena 2pe3*0m 5ang $#ha2#lan 2pe2#9# 0n30 2en5a%a 3e*2e703" Me3o$e #n# 7an5a $#g0naan a*ena:

• epa3 $an *ela3#9 m0*ah

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• Spe3*0m #n9*ame*ah 5ang $#ha2#lan oleh 20a30 2en5a%a a$alah ha2 $an oleh a*ena

#30 $apa3 men5a,#an 2e70ah 9#nge*p*#n3 >2#$# ,a*#@ 0n30 2en5a%a 3e*2e703"

Da*# $e*e3 Fo0*#e* 3e*2e703 #n3en2#3a2 gelom7ang $apa3 $#gam7a*an 2e7aga# $ae*ah %a30 a3a0 $ae*ah 9*e0en2#" Pe*07ahan gam7a*an #n3en2#3a2 gelom7ang *a$#a2# ele3*omagne3# $a*# $ae*ah %a30 e $ae*ah 9*e0en2# a3a0 2e7al#n5a $#2e703 T*an29o*ma2# Fo0*#e* >Fo0*#e* T*an29o*m@" Selan,03n5a pa$a 2#23#m op3# pe*ala3an #n23*0men FTIR $#paa# $a2a* $ae*ah %a30 5ang non $#2pe*2#9" Se7aga# 8on3oh apl#a2# pemaa#an gelom7ang *a$#a2# ele3*omagne3# 5ang 7e*$a2a*an $ae*ah %a30 a$alah

#n3e*9e*ome3e* 5ang $#em0aan oleh Al7e*3 A7*aham M#8hel2on >4a*,a$#; !??(@"

Se8a*a e2el0*0han; anal#2#2 mengg0naan Spe3*o9o3ome3e* FTIR mem#l## $0a ele7#han 03ama $#7an$#ngan me3o$a on=en2#onal la#nn5a; 5a#30 :

!" Dapa3 $#g0naan pa$a 2em0a 9*e%en2# $a*# 20m7e* 8aha5a 2e8a*a 2#m0l3an 2eh#ngga anal#2#2 $apa3 $#la0an le7#h 8epa3 $a*#pa$a mengg0naan 8a*a 2e0en2#al a3a0 28ann#ng"

&" Sen2#3#9#3a2 $a*# me3o$a Spe3*o9o3ome3*# FTIR le7#h 7e2a* $a*#pa$a 8a*a $#2pe*2#; 2e7a7 *a$#a2# 5ang ma20 e 2#23#m $e3e3o* le7#h 7an5a a*ena 3anpa ha*02 melal0# 8elah >2l#3le22@"

S#23em op3# Spe3*o9o3ome3e* FTIR $#lengap# $engan 8e*m#n 5ang 7e*ge*a 3ega l0*02 $an 8e*m#n 5ang $#am" Dengan $em##an *a$#a2# #n9*a me*ah aan men#m70lan pe*7e$aan ,a*a 5ang $#3emp0h men0,0 8e*m#n 5ang 7e*ge*a >M@ $an ,a*a 8e*m#n 5ang $#am >F@" Pe*7e$aan ,a*a 3emp0h *a$#a2# 3e*2e703 a$alah & 5ang 2elan,03n5a $#2e703 2e7aga# *e3a*$a2# >@" 4070ngan an3a*a #n3en2#3a2 *a$#a2# IR 5ang $#3e*#ma $e3e3o* 3e*ha$ap *e3a*$a2# $#2e703 2e7aga# #n3e*9e*og*am" Se$angan 2#23#m op3# $a*# Spe3*o9o3ome3e* IR 5ang $#$a2a*an a3a2 7ee*,an5a #n3e*9e*ome3e* $#2e703 2e7aga# 2#23em op3# Fo0*#e* T*an29o*m In9*a Re$"

S#23em op3# FTIR $#g0naan *a$#a2# LASER >L#gh3 Ampl#9#8a3#on 75 S3#m0la3e$ Emm#22#on o9 Ra$#a3#on@ 5ang 7e*90ng2# 2e7aga# *a$#a2# 5ang $##n3e*9e*en2#an $engan *a$#a2# #n9*a me*ah aga* 2#n5al *a$#a2# #n9*a me*ah 5ang $#3e*#ma oleh $e3e3o* 2e8a*a 030h $an le7#h 7a#"

De3e3o* 5ang $#g0naan $alam Spe3*o9o3ome3e* FTIR a$alah TGS >Te3*a Gl58e*#ne S0lpha3e@ a3a0 MT >Me*80*5 a$m#0m Tell0*#$e@" De3e3o* MT le7#h 7an5a

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$#g0naan a*ena mem#l## 7e7e*apa ele7#han $#7an$#ngan $e3e3o* TGS; 5a#30 mem7e*#an *e2pon 5ang le7#h 7a# pa$a 9*e%en2# mo$0la2# 3#ngg#; le7#h 2en2#3#9; le7#h 8epa3; 3#$a $#penga*0h# oleh 3empe*a30*; 2anga3 2ele3#9 3e*ha$ap ene*g# =#7*a2# 5ang $#3e*#ma $a*# *a$#a2# #n9*a me*ah >R023#na; &)).@"

III. Karakteristik Coffeinum / Kofein >FI I+; hal" &-'@

• Peme*#an: Se*70 p03#h a3a0 7en30 ,a*0m meng#la3 p03#h 7#a2an5a meng< g0mpal

3#$a 7e*7a0 *a2a pah#3" La*03an 7e*2#9a3 ne3*al 3e*ha$ap e*3a2 lam02" 6en30 h#$*a3n5a mea* $# 0$a*a"

• Kela*03an: Aga 20a* la*03 $alam a#*; $alam e3anol m0$ah la*03 $alam lo*o9o*m

20a* la*03 $alam e3e*"

• I$en3#9#a2#: Spe3*0m 2e*apan #n9*ame*ah a3 5ang 3elah $#e*#ngan $an

$#$#2pe*2#an $alam m#n5a m#ne*al men0n,0an ma2#m0m han5a pa$a pan,ang gelom7ang 5ang 2ama 2epe*3# pa$a o9e#n 6PFI"

IV. Cara Kerja

• Mengh#$0pan In23*0men

N5alaan Komp03e*  Tean 3om7ol ON pa$a #23*0men  In#2#al#2a2#  l# &H

pa$a So93%a*e Spe83*0m"

• M0la# S8an

!" Kl# #on Background  I2# nama Ko9e#n S3an$a*J  Le3aan Ko9e#n m0*n#

$#a3a2 pla3 UATR  Kl# #on Scan  M0n80l 2pe83*0m Ko9e#n S3an$a*J"

&" Kl# #on Background  I2# nama Ko9e#n SampelJ  Le3aan 2ampel

Ko9e#n ha2#l #2ola2# pla3 UATR  Kl# #on Scan  M0n80l 2pe83*0m Ko9e#n

SampelJ"

• P*#n3 2pe83*0m Ko9e#n S3an$a*$ $an Ko9e#n Sampel"

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Ia3an Ko9e#n 4a2#l I2ola2# Ko9e#n S3an$a*3 CO 23* !.?(;& 8m<! _{!.?';)? 8m}<! < 23* ?1!;?- 8m<! _{?1&;-) 8m}<! <4 7en$ !(-1;1) 8m<! _{!(-;!! 8m}<! <4 23* &?-.;& 8m<! _{&?-(;?- 8m}<! <N 23* !-'1;-- 8m<! _{!-'1;&( 8m}<! N<4 23* (!!!;'1 8m<! _{(!!!;' 8m}<! Spe*0m IR  Lamp#*an VI. Pembahasan

Pa$a p*a3#0m al# #n#; p*a3#an mela0an anal#2#2 3e*ha$ap o9e#n ha2#l #2ola2# $a*# $a0n 3eh $engan mengg0naan me3o$e 2pe3*o9o3ome3*# In9*a Me*ah" Pa$a p*a3#0m #n# $#g0naan ala3 2pe3*o9o3ome3e* FTIR" P*#n2#p e*,a 2pe3*o2op# FTIR

a$alah a$an5a #n3e*a2# ene*g# $engan ma3e*# a3a0 2e8a*a 0m0m $apa3 $#gam7a*an 2e7aga# 7e*#03: 2ampel $# 28an; 5ang 7e*a*3# 2#na* #n9*a me*ah aan $#le%a3an e 2ampel" Gelom7ang 5ang $#3e*02an oleh 2ampel aan $#3angap oleh $e3e3o* 5ang 3e*h070ng e 8omp03e* 5ang aan mem7e*#an gam7a*an 2pe83*0m 2ampel 5ang $#0,#" M#2alan $alam 20a30 pe*8o7aan 7e*0pa mole0l 2en5a%a omple2 5ang $#3em7a $engan ene*g# $a*# 20m7e* 2#na* 5ang aan men5e7a7an mole0l 3e*2e703 mengalam# =#7*a2#" S0m7e* 2#na* 5ang $#g0naan a$alah e*am#; 5ang apa7#la $#al#*# a*02 l#23*# maa e*am# #n# $apa3 meman8a*an #n9*a me*ah" +#7*a2# $apa3 3e*,a$# a*ena ene*g# 5ang 7e*a2al $a*# 2#na* #n9*a me*ah 3#$a 800p 0a3 0n30 men5e7a7an 3e*,a$#n5a a3om#2a2# a3a0p0n e2#3a2# ele83*on pa$a mole0l 2en5a%a 5ang $#3em7a $#mana 7e2a*n5a ene*g# =#7*a2# 3#ap a3om a3a0 mole0l 7e*7e$a 3e*gan30ng pa$a a3om<a3om $an e0a3an #a3an 5ang mengh070ngann5a 2eh#ngga $#ha2#lan 9*e0en2# 5ang 7e*7e$a p0la"

T0,0an $a*# p*a3#0m #n# a$alah 0n30 mem703#an apaah o9e#n ha2#l #2ola2# $a*# $a0n 3eh 3e*2e703 a$alah 7ena* o9e#n m0*n#" 4a2#l 2pe3*0m IR $a*# o9e#n #2ola2# $#7an$#ngan $engan ha2#l 2pe3*0m o9e#n 23an$a*3" Da*# pe*7an$#ngan e$0an5a $#$apa3an ha2#l e8o8oan 2e7e2a* ?. $engan p*o9#l 2pe3*0m pa$a $ae*ah 9#ngge* p*#n3 5ang 2ama" Ke8o8oan p*o9#l 2pe3*0m an3a*a o9e#n #2ola2# $an o9e#n 23an$a*3

mem703#an 7ah%a 2en5a%a 5ang $#anal#2a a$alah 7ena* 2en5a%a o9e#n" Aan 3e3ap#; 3e*$apa3 2e$##3 pe*7e$aan pa$a $ae*ah &?&);) 8m<! _{ &-);1- 8m}<! _{$#mana pa$a}

2pe3*0m o9e#n 23an$a*3 3#$a a$a g0g02 5ang m0n80l pa$a $ae*ah 3e*2e703" Da*# ha2#l anal#2a; p*a3#an men$0ga 7ah%a g0g02 3e*2e703 a$alah g0g02 alana" G0g02 a2#ng

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3e*2e703 em0ng#nan 7e*a2al $a*# peng0apan 4l( 5ang 0*ang 2emp0*na" Seh#ngga

o9e#n 5ang $#ha2#lan $a*# #2ola2# $a0n 3eh 3e*2e703 0*ang m0*n#"

VII. Kesimpulan

• Anal#2#2 2en5a%a Ko9e#n ha2#l #2ola2# $a*# $a0n 3eh $engan Me3o$e Spe3*o9o3ome3*#

FTIR men0n,0an ha2#l 9#nge*p*#n3 5ang 2e20a# $engan 2pe*0m IR $a*# o9e#n 23an$a*3"

• Te*$apa3 g0g02 a2#ng 5ang m080l pa$a $ae*ah &?&);) 8m<! 5ang $#$0ga a$alah

g0g02 alana"

VIII. Daftar Pustaka

6a22e3; " !??'" 600 A,a* +ogel K#m#a Anal#2#2 K0an3#3a3#9 Ano*gan#" Pene*7#3 600 Ke$o3e*an EG" aa*3a"

Fa*maope In$one2#a e$#2# I+"

G#%anga*a S; EG"; &)).; Apl#a2# Log#a S5a*a9 F05 Pa$a Anal#2#2 S#$# a*# M#n5a 60m# Mengg0naan Spe3*o9o3ome3e* In9*a Me*ah  T*an29o*ma2# Fo0*#e* >FT< IR@J; Seolah T#ngg# Ene*g# $an M#ne*al; ep0  a%a Tengah"

4a*,a$#; W" !??(" Ilm0 K#m#a Da2a* Anal#3#" E*langga" aa*3a"