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PENGARUH EKSTRAK JAHE (Zingiber officinale Rosc.) var. Gajah TERHADAP KUALITAS SPERMATOZOA MENCIT (Mus musculus) YANG TERPAPAR 2-METHOXYETHANOL Repository - UNAIR REPOSITORY

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Lampiran 1. Data Hasil Pengamatan Jumlah Spermatozoa Mencit Pada

Jumlah spermatozoa (106 sel/ml)

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Lampiran 5. Data Hasil Pengamatan Motilitas Spermatozoa Mencit Pada Perlakuan 1

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Lampiran 7. Data Hasil Pengamatan Motilitas Spermatozoa Mencit Pada Perlakuan 3

Perlakuan

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Lampiran 9. Hasil Analisis Data Jumlah Spermatozoa Mencit

Normal Parametersa , b Mean 4.7153

Std. Deviation .66219

Most Extreme Differences Absolute .217

Positive .112

Negative -.217

Kolmogorov-Smirnov Z 1.190

Asymp. Sig. (2-tailed) .118

a. Test distribution is Normal.

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ANOVA

Jumlah Spermatozoa

Sum of Squares df Mean Square F Sig.

Between Groups 11.019 4 2.755 40.572 .000

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Lampiran 10. Hasil Analisis Data Morfologi Spermatozoa Mencit

NPar Tests

One-Sample Kolmogorov-Smirnov Test

Morfologi Spermatozoa

N 30

Normal Parametersa , b Mean 67.0133

Std. Deviation 8.32792

Most Extreme Differences Absolute .242

Positive .163

Negative -.242

Kolmogorov-Smirnov Z 1.326

Asymp. Sig. (2-tailed) .060

a. Test distribution is Normal. b. Calculated from data. Total 30 67.0133 8.32792 1.52046 63.9036 70.1230 50.20 75.40

Test of Homogeneity of Variances

Morfologi_spermatozoa

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ANOVA

Morfologi Spermatozoa

Sum of Squares df Mean Square F Sig. Between Groups 1957.581 4 489.395 227.866 .000

Within Groups 53.693 25 2.148

P1 .23333 .84612 .785 -1.5093 1.9759

P2 1.60000 .84612 .070 -.1426 3.3426

P3 6.70000* .84612 .000 4.9574 8.4426

K1 K0 -21.40000* .84612 .000 -23.1426 -19.6574 P1 -21.16667* .84612 .000 -22.9093 -19.4241 P2 -19.80000* .84612 .000 -21.5426 -18.0574 P3 -14.70000* .84612 .000 -16.4426 -12.9574

P1 K0 -.23333 .84612 .785 -1.9759 1.5093

K1 21.16667* .84612 .000 19.4241 22.9093

P2 1.36667 .84612 .119 -.3759 3.1093

P3 6.46667* .84612 .000 4.7241 8.2093

P2 K0 -1.60000 .84612 .070 -3.3426 .1426

K1 19.80000* .84612 .000 18.0574 21.5426

P1 -1.36667 .84612 .119 -3.1093 .3759

P3 5.10000* .84612 .000 3.3574 6.8426

P3 K0 -6.70000* .84612 .000 -8.4426 -4.9574

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Lampiran 11. Hasil Analisis Data Motilitas Spermatozoa Mencit

NPar Tests

One-Sample Kolmogorov-Smirnov Test

Motilitas Spermatozoa

N 30

Normal Parametersa , b Mean 6.7125

Std. Deviation 1.20843

Most Extreme Differences Absolute .241

Positive .152

Negative -.241

Kolmogorov-Smirnov Z 1.320

Asymp. Sig. (2-tailed) .061

a. Test distribution is Normal. b. Calculated from data.

Total 30 6.7125 1.20843 .22063 6.2613 7.1638 4.07 7.95

Test of Homogeneity of Variances

Motilitas Spermatozoa

Levene Statistic df1 df2 Sig.

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ANOVA

Motilitas Spermatozoa

Sum of Squares df Mean Square F Sig.

Between Groups 39.879 4 9.970 100.936 .000

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Lampiran 12. Hasil Analisis Data Viabilitas Spermatozoa Mencit

NPar Tests

One-Sample Kolmogorov-Smirnov Test

Viabilitas Spermatozoa

N 30

Normal Parametersa , b Mean 64.7267

Std. Deviation 7.25548

Most Extreme Differences Absolute .233

Positive .186

Negative -.233

Kolmogorov-Smirnov Z 1.278

Asymp. Sig. (2-tailed) .076

a. Test distribution is Normal. b. Calculated from data

K0 6 70.0000 1.24419 .50794 68.6943 71.3057 68.00 71.20 K1 6 51.1333 1.23234 .50310 49.8401 52.4266 50.00 52.90 P1 6 69.0667 .74476 .30405 68.2851 69.8482 68.00 70.00 P2 6 68.7000 1.74356 .71181 66.8702 70.5298 65.80 70.10 P3 6 64.7333 1.41798 .57889 63.2453 66.2214 63.00 67.20 Total 30 64.7267 7.25548 1.32466 62.0174 67.4359 50.00 71.20

Test of Homogeneity of Variances

Viabilitas Spermatozoa

Levene Statistic df1 df2 Sig.

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ANOVA

Viabilitas Spermatozoa

Sum of Squares df Mean Square F Sig.

Between Groups 1483.259 4 370.815 213.800 .000

Within Groups 43.360 25 1.734

P1 .93333 .76035 .231 -.6326 2.4993

P2 1.30000 .76035 .100 -.2660 2.8660

P3 5.26667* .76035 .000 3.7007 6.8326

K1 K0 -18.86667* .76035 .000 -20.4326 -17.3007 P1 -17.93333* .76035 .000 -19.4993 -16.3674 P2 -17.56667* .76035 .000 -19.1326 -16.0007 P3 -13.60000* .76035 .000 -15.1660 -12.0340

P1 K0 -.93333 .76035 .231 -2.4993 .6326

K1 17.93333* .76035 .000 16.3674 19.4993

P2 .36667 .76035 .634 -1.1993 1.9326

P3 4.33333* .76035 .000 2.7674 5.8993

P2 K0 -1.30000 .76035 .100 -2.8660 .2660

K1 17.56667* .76035 .000 16.0007 19.1326

P1 -.36667 .76035 .634 -1.9326 1.1993

P3 3.96667* .76035 .000 2.4007 5.5326

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Lampiran 13. Foto – Foto Penelitian

Mencit (

Mus musculus

) jantan

A.

Bak bedah, B. Gunting bedah, C. Pisau bedah, D. Pinset, E. Spatula,

F. Pipet tetes, G.

Object glass

, H. Hemositometer, I.

Hand counter

,

J

I H

G F

E

B

D C

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Larutan pewarna 1% Eosin dan 10% Negrosin

Mikroskop cahaya (

Olympus

)

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