53 BAB V
KESIMPULAN DAN SARAN
5.1 Kesimpulan
1. Senyawa alkaloid hasil isolasi daun Pule (Alstonia scholaris) adalah suatu alkaloid indol golongan kerangka aspidodasikarpin
2. Senyawa alkaloid dari daun Alstonia scholaris memiliki aktivitas sebagai antiplasmodial dalam uji in vitro terhadap Plasmodium falciparum dengan nilai IC50sebesar 1,926 μg/ml
5.2 Saran
Disarankan agar dilakukan uji aktivitas antimalaria terhadap senyawa murni alkaloid indol golongan kerangka aspidodasikarpin untuk mengetahui aktivitas antimalarianya. Sehingga senyawa alkaloid ini dapat digunakan sebagai obat antimalaria.
N H
N O
CO2CH3
H
H H
O OCH3
DAFTAR PUSTAKA
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Lampiran 1
Spektrum senyawa B
Spektrum 13C-NMR
Spektrum IR
Lampiran 2
Hasil Uji Antiplasmodial Ekstrak Alkaloid daun Alstonia scholaris Konsentrasi
uji (μg/ml) R 0 jam% Parasitemia48 jam % Pertumbuhan % Hambatan % Hambatan rata-rata
Kontrol (-) 1 1,2 3,80 2,60 -
-2 1,3 2,90 1,60
-100 1 1,2 0,70 0 100 100
2 1,3 1,10 0 100
10 1 1,2 1,80 0,60 76,92 77,21
2 1,3 1,66 0,36 77,50
1 1 1,2 2,40 1,20 53,85 55,05
2 1,3 2,00 0,70 56,25
0,1 1 1,2 2,60 1,40 46,15 44,95
2 1,3 2,20 0,90 43,75
0,01 1 1,2 3,20 2,00 23,07 24,04
Probit Ekstrak Alkaloid daun Alstonia scholaris
PROBIT Konsentrasi OF total WITH penghambatan /LOG 10 /MODEL PROBIT /PRINT FREQ CI /CRITERIA P(0.15) ITERATE(20) STEPLIMIT(.1).
Data Information Number of Responses >
Number of Subjects
a. Parameter estimates did not converge.
Parameter Estimates
Parameter Estimate Std. Error Z Sig.
95% Confidence Interval Lower Bound Upper Bound
PROBITa penghambatan 24.313 2.343 10.375 .000 19.720 28.906
Intercept -46.824 4.487 -10.436 .000 -51.311 -42.337
Chi-Square Tests
Chi-Square dfa Sig. PROBIT Pearson
Goodness-of-Fit Test
4.381E33 3 .000b
a. Statistics based on individual cases differ from statistics based on aggregated cases.
b. Since the significance level is less than .150, a heterogeneity factor is used in the calculation of confidence limits.
Cell Counts and Residuals
PROBIT 1 2.000 100 100 96.425 3.575 .964
2 1.888 100 10 17.651 -7.651 .177
3 1.741 100 1 .000 1.000 .000
95% Confidence Limits for penghambatan
.150 76.427 . . 1.883 . .
.200 77.850 . . 1.891 . .
.250 79.092 . . 1.898 . .
.300 80.224 . . 1.904 . .
.350 81.288 . . 1.910 . .
.400 82.310 . . 1.915 . .
.450 83.311 . . 1.921 . .
.500 84.309 . . 1.926 . .
.550 85.318 . . 1.931 . .
.600 86.356 . . 1.936 . .
.650 87.442 . . 1.942 . .
.700 88.602 . . 1.947 . .
.750 89.870 . . 1.954 . .
.800 91.304 . . 1.960 . .
.850 93.004 . . 1.969 . .
.900 95.188 . . 1.979 . .
.910 95.723 . . 1.981 . .
.920 96.308 . . 1.984 . .
.930 96.955 . . 1.987 . .
.940 97.683 . . 1.990 . .
.950 98.520 . . 1.994 . .
.960 99.513 . . 1.998 . .
.970 100.747 . . 2.003 . .
.980 102.410 . . 2.010 . .
.990 105.089 . . 2.022 . .
