Antioxidant Enzyme Activities In Various Tissue On Rat Under Hyperglycemia Condition.

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BIOSCITNOIS

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BIOIICHNOI,OGY

PROGRAITI

AND ABSTRACT BOOK

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P-3-17

Aniioxidant Enzvme Adivities ln vario!s Tissue On Rat

Under HYPerglYcemia condition

I NYOMAN SUARSANAI, BAMBANG PONTIO PRIOSOERYANTO2,

TUTIK WREsDIYATF, MARIA BtNTANG3

LLaboratorV

of Bjochemisry, Facultv of Veterinarv Medlcine, Udayana

UnlversltY

ll. PB. Sudirman Denpasar, Ba

l

s!anana65.avahoo com

'zFacu

tt

ofveterlnary Medicine, BogorAgrlcuLture lnstitute, Bogor

3

Laboratory of Biochemlstrv, Facultvof Mathernatlcs and Naturalsclences Bogor

Abstract

oxidative stress has been considercd by manv as an explanaton

for

the

ussue darnage that accompanles chro.lc hvperSlycernla chronlc exposure

to

hyperelyc;mia can ead

to

Seneration of free radica

s

and decreased

iitla:e

Lular antioxidant that cautins cel ular dvsfLncuon This nud\/ aimed

to

determine the leve s

of

lntracel uLar antioxidant

in

ervthrocvte, iver,

kidn€y, and spteen tissue of rat under hvperslvcemia condiuons This studv

was used 10 male rats spr:que Dawlv aveGge bodv welcht of 200

g

Thev were sub divided into

two

groups; (1) a controL 8roup, wjthout treatment'

and (2)

a

hypeGlyceniia Sroup was

tre:ted

bv

aloxan

120 mg/k8 bw intraperitoneally. The treatment was conducted for 28 days The rats were

given commercialfeed and water ad ljbjturn

Attheendoftreatmentalol

iats were eutlanasia with cethamine-HCl Erythroc\/te, iver, kldnev, and

sn een tissle were co lected for analvsis of lntrace lular anuoxidant enzvme

tlvets using spectroptrotometric rnethod. The resu ts showed ihat the liver

t*r"

.ont"inlnC

intrac€Llular antioxldant enzyrnes'

ie

superox de

ditmutase (soD),

glltahion

peroxidase (GPx)

and

catalas€ hichest

compared with erythroctte, kidnev, and spleen tissue whlle erYthroc!4e

k

tissue

that the

least

iitracel!!lar

antioxldant enzymes Hypergycemia condiuons cause intracellular antioxldant enzvme eve s decr€ase

at

all Ussue. Amorg

the

intracellular antioxjdant enzvmes, SOD en'zyme was

detreased highest

in

al

tissues when cornpared

to

other

antloxdant eizymes urder hVperglycemia condltion

(eV wordsi

Artioxidant, superoxlde dCmuta5e,

guthation

peroxdase,

ffi