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PENGARUH PENAMBAHAN MINYAK BIJI ANGGUR (Grape Seed Oil) TERHADAP EFEKTIVITAS SEDIAAN TABIR SURYA
KOMBINASI OKSIBENZON DAN OKTILMETOKSINAMAT DALAM BASIS VANISHING CREAM
ABSTRAK
Latar belakang: Minyak biji anggur/ grape seed oil (MBA) merupakan salah satu minyak nabati yang sering digunakan dalam industri kosmetik, kuliner, serta untuk tujuan farmasetis maupun medis. Minyak biji anggur mengandung asam lemak tidak jenuh berupa asam linoleat dan asam linolenat dalam konsentrasi tinggi. Minyak biji anggur juga mengandung senyawa fenolik, tokoferol, serta tannin yang dapat melindungi kulit dari bahaya radiasi ultraviolet matahari. Tujuan dari penelitian ini adalah untuk melihat pengaruh penambahan minyak biji anggur terhadap peningkatan efektivitas sediaan tabir surya kombinasi oksibenzon dan oktilmetoksisinamat (2 dan 5 % b/b) dengan parameter nilai SPF (Sun Protection Factor).
Metode: Sediaan dibuat dalam 5 formula yaitu formula A (blanko), formula B (6% MBA), formula C (8% MBA), formula D (10% MBA), formula E (12% MBA) yang dalam formulanya masing-masing mengandung oksibenzon dan oktilmetoksisinamat (2 dan 5 % b/b). Evaluasi sediaan krim dilakukan terhadap homogenitas, tipe emulsi, pH, stabilitas (warna dan bau), iritasi kulit, dan efektivitas SPF sediaan secara in vitro menggunakan spektrofotometer UV. Selanjutnya nilai SPF yang diperoleh diuji dengan One Way Anova untuk mengetahui apakah terdapat perbedaan yang signifikan diantara kelima formula. Hasil: Hasil pengujian menunjukkan seluruh sampel homogen dengan tipe emulsi m/a, rentang pH pada kisaran 6,53 - 6,56, tidak menyebabkan iritasi dan sediaan stabil selama penyimpanan 12 minggu pada suhu kamar. Hasil penelitian juga menunjukkan bahwa nilai SPF sediaan, meningkat seiring dengan meningkatnya konsentrasi minyak biji anggur yang ditambahkan dengan nilai SPF rata-rata dari masing-masing formula sebagai berikut: formula A 11,0714; formula B 11,9054; formula C 12,3189; formula D 12,7222; serta formula E 13,0343. Kelima formula tersebut dapat digunakan secara optimal sebagai tabir surya dengan kategori proteksi maksimal. Dari hasil uji statistik diketahui bahwa terdapat perbedaan yang signifikan terhadap nilai SPF kelima formula sediaan. Kesimpulan: Dari penelitian yang dilakukan dapat disimpulkan bahwa dengan penambahan minyak biji anggur dapat meningkatkan efektivitas sediaan tabir surya dari oksibenzon dan oktilmetoksisinamat dalam basis vanishing cream.
Kata kunci: Tabir surya, minyak biji anggur, oksibenzon, oktilmetoksisinamat, SPF (Sun Protection Factor)
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THE INFLUENCE OF GRAPE SEED OIL AGAINST THE EFFECTIVENESS OF SUNSCREEN PREPARATIONS CONTAINING
THE COMBINATION OF OXYBENZONE AND
OCTILMETOXYCINNAMATE IN VANISHING CREAM BASIS
ABSTRACT
Background: Grape seed oil is a vegetable oil that is usually used i n cosmetic industry, culinary, pharmaceutic and also medical used. Grape seed oil contains of unsaturated fatty acid such as linoleic acid and linolenic acid in high concentration. Grape seed oil also contain phenolic compound, tocoferol and tannin which can protect skin from the impact of sun’s ultraviolet radiation. Purpose: The aim of this study was to evaluate the influence of adding grape seed oil to increase the effectiveness of sunscreen preparations containing the combination of oxybenzone and octylmetoxycinnamate (2 and 5 % b/b) by measuring the SPF (Sun Protection Factor) value as its parameter.
Methods: In this study, the preparations were made in 5 formulas such as formula A (blank), formula B (6% GSO), formula C (8% GSO), formula D (10% GSO), formula E (12% GSO), combine with oxybenzone and octylmetoxycinnamate (2 and 5 % b/b) in each formulas. Evaluations conducted on cream homogenity, emulsion type, pH, stability, skin irritation, and determining the SPF value of the preparations by in vitro way using spectrophotometer UV. Then the value of SPF will be tested in One Way Anova to see whether there is any significant difference in those formulas.
Result: The evaluation results showed that all sample homogen with emulsion type w/o, in range 6.53 – 6.56 pH, non-irritating, and a stable preparation during 12 weeks of storage at room temperature. The evaluation also showed that the preparation’s SPF value increases along with the increased of additional grape seed oil concentration, the average SPF value from each formula were : formula A 11.0714; formula B 11.9054; formula C 12.3189; formula D 12.7222; and formula E 13.0343. These formulas can be used optimally as sunscreen with maximal protection category. From the result of statistic tests, there was a significant difference on the SPF value between 5 formulas’ preparation.
Conclusion: From the evaluation, be concluded that with additional of grape seed oil can increase the effectiveness sunscreen preparation from octylmetoxycinnamate and oxybenzone in vanishing cream basis.
