7. LAMPIRAN
Lampiran 1. Diagram Alir Penelitian Pendahuluan
Dilakukan proses inkubasi pada suhu 37°C pada
hotplate dan diaduk dengan stirrer
Inkubasi selama 30
menit
Inkubasi selama 60
menit
Inkubasi selama 90
menit
Dimasukkan ke dalam
tabung reaksi
Disentrifugasi pada kecepatan
5000 rpm selama 30 menit
Fraksi tidak terlarut
Fraksi terlarut
Pengujian kandungan logam kadmium dan tembaga (AAS)
Destruksi logam
1 gram ekstrak pektin
dari masing-masing
daun bayam
1 ml larutan logam
tembaga atau kadmium
50 ml larutan
buffer natrium
bikarbonat
Lampiran 2. Diagram Alir Penelitian Utama
Dimasukkan ke dalam
tabung reaksi
Disentrifugasi pada kecepatan
5000 rpm selama 30 menit
Fraksi tidak terlarut
Fraksi terlarut
Pengujian kandungan logam kadmium dan tembaga (AAS)
Destruksi logam
1 gram ekstrak pektin
dari masing-masing
daun bayam
1 ml larutan logam
tembaga atau kadmium
50 ml larutan
buffer natrium
bikarbonat
Dilakukan proses inkubasi pada suhu 37°C pada
hotplate dan diaduk dengan stirrer
Inkubasi selama 60
menit
Inkubasi selama 90
menit
Inkubasi selama 120
menit
Lampiran 3. Tabel Uji Normalitas Penyerapan Pektin Daun Bayam Terhadap
Logam Kadmium (Cd)
Tests of Normal ity
.189 5 .200* .933 5 .615 .253 5 .200* .892 5 .369 .201 5 .200* .942 5 .678 .173 5 .200* .971 5 .882 .204 5 .200* .946 5 .707 .202 5 .200* .924 5 .553 .199 5 .200* .912 5 .481 .222 5 .200* .908 5 .456 .153 5 .200* .988 5 .972 .217 5 .200* .888 5 .348 .209 5 .200* .966 5 .846 .186 5 .200* .930 5 .593 .178 5 .200* .982 5 .946 .262 5 .200* .914 5 .495 .204 5 .200* .943 5 .689 .183 5 .200* .948 5 .724 .243 5 .200* .944 5 .695 .184 5 .200* .950 5 .738 .200 5 .200* .943 5 .685 .136 5 .200* .987 5 .967 .266 5 .200* .889 5 .354 .202 5 .200* .933 5 .615 .180 5 .200* .952 5 .753 .246 5 .200* .924 5 .556 .219 5 .200* .916 5 .501 .198 5 .200* .929 5 .588 .206 5 .200* .886 5 .336 .261 5 .200* .951 5 .741 .293 5 .185 .778 5 .053 .201 5 .200* .978 5 .922 .154 5 .200* .969 5 .870 .270 5 .200* .860 5 .229 .296 5 .175 .737 5 .022 .239 5 .200* .926 5 .567 .202 5 .200* .921 5 .534 .294 5 .183 .920 5 .530 Kombinasi PDBS 60 Menit PDBS 90 Menit PDBS 120 Menit PDBM 60 Menit PDBM 90 Menit PDBM 120 Menit PDBK 60 Menit PDBK 90 Menit PDBK 120 Menit PK 60 Menit PK 90 Menit PK 120 Menit PDBS 60 Menit PDBS 90 Menit PDBS 120 Menit PDBM 60 Menit PDBM 90 Menit PDBM 120 Menit PDBK 60 Menit PDBK 90 Menit PDBK 120 Menit PK 60 Menit PK 90 Menit PK 120 Menit PDBS 60 Menit PDBS 90 Menit PDBS 120 Menit PDBM 60 Menit PDBM 90 Menit PDBM 120 Menit PDBK 60 Menit PDBK 90 Menit PDBK 120 Menit PK 60 Menit PK 90 Menit PK 120 Menit Cd_Padat Cd_Cair Cd_Total
Stat is tic df Sig. Stat is tic df Sig. Kolmogorov -Smirnova Shapiro-Wilk
This is a lower bound of t he t rue signif icance. *.
Lillief ors Signif ic ance Correct ion a.
Lampiran 4. Tabel Uji Normalitas Penyerapan Pektin Daun Bayam Terhadap
Logam Tembaga (Cu)
Tests of Normal ity
.223 5 .200* .957 5 .787 .180 5 .200* .967 5 .859 .157 5 .200* .990 5 .979 .234 5 .200* .883 5 .324 .319 5 .107 .861 5 .233 .248 5 .200* .929 5 .588 .220 5 .200* .869 5 .263 .286 5 .200* .813 5 .103 .212 5 .200* .932 5 .608 .226 5 .200* .946 5 .707 .262 5 .200* .866 5 .250 .330 5 .079 .735 5 .021 .158 5 .200* .981 5 .939 .130 5 .200* .994 5 .992 .200 5 .200* .946 5 .711 .306 5 .143 .757 5 .035 .189 5 .200* .980 5 .935 .292 5 .188 .895 5 .384 .227 5 .200* .960 5 .805 .251 5 .200* .896 5 .391 .178 5 .200* .958 5 .794 .323 5 .095 .896 5 .389 .222 5 .200* .911 5 .471 .211 5 .200* .942 5 .682 .147 5 .200* .987 5 .970 .246 5 .200* .922 5 .543 .281 5 .200* .881 5 .312 .270 5 .200* .879 5 .303 .291 5 .191 .862 5 .236 .270 5 .200* .910 5 .465 .165 5 .200* .966 5 .848 .247 5 .200* .834 5 .149 .207 5 .200* .927 5 .574 .213 5 .200* .919 5 .520 .386 5 .014 .716 5 .014 .361 5 .032 .725 5 .017 Kombinasi PDBS 60 Menit PDBS 90 Menit PDBS 120 Menit PDBM 60 Menit PDBM 90 Menit PDBM 120 Menit PDBK 60 Menit PDBK 90 Menit PDBK 120 Menit PK 60 Menit PK 90 Menit PK 120 Menit PDBS 60 Menit PDBS 90 Menit PDBS 120 Menit PDBM 60 Menit PDBM 90 Menit PDBM 120 Menit PDBK 60 Menit PDBK 90 Menit PDBK 120 Menit PK 60 Menit PK 90 Menit PK 120 Menit PDBS 60 Menit PDBS 90 Menit PDBS 120 Menit PDBM 60 Menit PDBM 90 Menit PDBM 120 Menit PDBK 60 Menit PDBK 90 Menit PDBK 120 Menit PK 60 Menit PK 90 Menit PK 120 Menit Cu_Padat Cu_Cair Cu_Total
Stat is tic df Sig. Stat is tic df Sig. Kolmogorov -Smirnova Shapiro-Wilk
This is a lower bound of t he t rue signif icance. *.
Lillief ors Signif ic ance Correct ion a.
Lampiran 5. Tabel Uji Beda Pektin Daun Bayam Terhadap Logam Kadmium (Cd)
oleh Fraksi Tidak Terlarut Antara Waktu Kontak dan Varietas
Lampiran 6. Tabel Uji Beda Pektin Daun Bayam Terhadap Logam Kadmium (Cd)
oleh Fraksi Terlarut Antara Waktu Kontak dan Varietas
Cd_Padat Duncana 5 104.2002 5 106.9020 5 118.9590 5 129.4018 5 129.9740 129.9740 5 133.1170 5 139.2850 5 153.3696 5 154.1208 5 161.8936 5 163.9310 5 180.1870 .106 1. 000 .729 .061 1. 000 .649 .220 1. 000 Kombinasi PK 120 Menit PDBK 60 Menit PK 90 Menit PK 60 Menit PDBK 90 Menit PDBK 120 Menit PDBM 60 Menit PDBS 60 Menit PDBM 90 Menit PDBS 90 Menit PDBM 120 Menit PDBS 120 Menit Sig. N 1 2 3 4 5 6 7 8 Subset f or alpha = .05
Means f or groups in homogeneous s ubset s are display ed. Uses Harmonic Mean Sample Size = 5.000. a.
Cd_Cai r
Duncan
a5
15. 1554
5
16. 0202
5
16. 7470
5
17. 0826
5
21. 6886
5
22. 5514
5
24. 5918
5
24. 9542
5
25. 4050
5
30. 4260
5
31. 3560
5
36. 6570
.054
.342
.400
.306
1. 000
Kombinasi
PK 60 Menit
PDBM 60 Menit
PK 90 Menit
PK 120 Menit
PDBS 60 Menit
PDBM 90 Menit
PDBS 90 Menit
PDBK 60 Menit
PDBM 120 Menit
PDBK 90 Menit
PDBS 120 Menit
PDBK 120 Menit
Sig.
N
1
2
3
4
5
Subset f or alpha = .05
Means f or groups in homogeneous s ubsets are display ed.
Uses Harmonic Mean Sample Size = 5. 000.
Lampiran 7. Tabel Uji Beda Pektin Daun Bayam Terhadap Logam Tembaga (Cu)
oleh Fraksi Tidak Terlarut Antara Waktu Kontak dan Varietas
Lampiran 8. Tabel Uji Beda Pektin Daun Bayam Terhadap Logam Tembaga (Cu)
oleh Fraksi Terlarut Antara Waktu Kontak dan Varietas
Cu_Padat Duncana 5 87. 7130 5 96. 5460 5 98. 8650 5 99. 8388 99. 8388 5 104.9768 104.9768 5 109.2986 5 109.4020 5 127.1262 5 129.3414 5 137.7958 5 143.0412 5 158.9310 1. 000 .288 .081 .154 .446 .075 1. 000 Kombinasi PDBS 60 Menit PDBS 90 Menit PK 120 Menit PK 60 Menit PK 90 Menit PDBK 90 Menit PDBK 60 Menit PDBS 120 Menit PDBM 60 Menit PDBK 120 Menit PDBM 90 Menit PDBM 120 Menit Sig. N 1 2 3 4 5 6 7 Subset f or alpha = .05
Means f or groups in homogeneous s ubset s are display ed. Uses Harmonic Mean Sample Size = 5.000.
a. Cu_Cai r Duncana 5 8. 4074 5 10. 0150 10. 0150 5 10. 8950 5 13. 7188 5 14. 0676 5 15. 1834 5 15. 3600 5 17. 1602 5 17. 3710 5 19. 3282 5 19. 6218 5 19. 8922 .073 .321 .093 .811 .550 Kombinasi PDBM 60 Menit PDBM 120 Menit PDBM 90 Menit PDBS 60 Menit PDBS 90 Menit PDBK 60 Menit PDBS 120 Menit PK 120 Menit PDBK 90 Menit PK 60 Menit PK 90 Menit PDBK 120 Menit Sig. N 1 2 3 4 5 Subset f or alpha = .05
Means f or groups in homogeneous s ubsets are display ed. Uses Harmonic Mean Sam ple Size = 5. 000.
Lampiran 9. Tabel Uji ANOVA Penyerapan Pektin Daun Bayam Terhadap
Logam Kadmium (Cd)
Lampiran 10. Tabel Uji ANOVA Penyerapan Pektin Daun Bayam Terhadap
Logam Tembaga (Cu)
Test of Homogenei ty of Variances
1. 388 11 48 .210
.907 11 48 .541
Cd_Padat Cd_Cair
Lev ene
Stat is tic df 1 df 2 Sig.
ANOVA 30619.541 11 2783.595 413.868 .000 322.839 48 6. 726 30942.380 59 2531.209 11 230.110 114.064 .000 96. 834 48 2. 017 2628.043 59 Between Groups Within Groups Tot al Between Groups Within Groups Tot al Cd_Padat Cd_Cair Sum of
Squares df Mean Square F Sig.
Test of Homogenei ty of Variances
1. 548 11 48 .146
.885 11 48 .561
Cu_Padat Cu_Cair
Lev ene
Stat is tic df 1 df 2 Sig.
ANOVA 26423.205 11 2402.110 115.486 .000 998.400 48 20. 800 27421.605 59 810.335 11 73. 667 38. 272 .000 92. 391 48 1. 925 902.726 59 Between Groups Within Groups Tot al Between Groups Within Groups Tot al Cu_Padat Cu_Cair Sum of
