Bab V Penutup
V.2 Saran
25
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LAMPIRAN
30 Lampiran A. Kurva Standar
A.1 Tabel data pengukuran absorbansi standar BSA (595nm)
[BSA] (µg/mL) A1 A2 A3 A rata-rata
25 0,761 0,779 0,771 0,770
20 0,681 0,671 0,697 0,683
15 0,493 0,501 0,497 0,497
10 0,382 0,379 0,378 0,380
5 0,225 0,228 0,228 0,227
2,5 0,137 0,138 0,137 0,137
A.2 Kurva standar BSA
31 Lampiran B. Kurva standar
B.1 Tabel data pengukuran absorbansi standar PNP (405nm)
[PNP] (µg/mL) A1 A2 A3 A rata-rata
0 0 0 0 0
2 0,216 0,221 0,211 0,216
3 0,264 0,266 0,265 0,265
4 0,355 0,334 0,379 0,356
5 0,433 0,424 0,442 0,433
6 0,517 0,499 0,535 0,517
7 0,621 0,603 0,639 0,621
8 0,707 0,692 0,722 0,707
B.2 Kurva standar PNP
32 Lampiran C Kurva Standar
C.1 Tabel data kurva pertumbuhan dan kurva aktivitas enzim lipase
Kontrol Sampel Abs Aktivitas Jam Aktivitas / 10-3 OD
0,1905 0,1995 0,0090 54,4653 1 0,0544 0,0225
0,1905 0,2090 0,0185 135,3122 2 0,1353 0,0245
0,1900 0,2130 0,0230 173,6081 3 0,1736 0,0250
0,1890 0,2185 0,0295 228,9244 4 0,2289 0,0265
0,1880 0,2340 0,0460 369,3428 5 0,3693 0,0350
0,1870 0,2370 0,0500 403,3836 6 0,4033 0,0705
0,1860 0,2375 0,0515 416,1489 7 0,4161 0,1990
0,1825 0,2365 0,0540 437,4244 8 0,4374 0,4835
0,1795 0,2510 0,0715 586,3530 9 0,5863 0,7405
0,1765 0,2515 0,0750 616,1387 10 0,6161 0,7945
0,1710 0,2530 0,0820 675,7102 11 0,6757 0,8470
0,1675 0,2540 0,0865 714,0061 12 0,7140 0,8480
0,1620 0,2560 0,0940 777,8326 13 0,7778 0,8935
0,1590 0,2575 0,0985 816,1285 14 0,8161 0,9140
0,1530 0,2575 0,1045 867,1898 15 0,8671 1,0270
0,1525 0,2615 0,1090 905,4857 16 0,9054 1,0690
0,1505 0,2745 0,1240 1033,1388 17 1,0331 1,0590
0,1475 0,2700 0,1225 1020,3735 18 1,0203 1,0270
0,1440 0,2650 0,1210 1007,6082 19 1,0076 0,9890
0,1430 0,2615 0,1185 986,3326 20 0,9863 0,9880
0,139 0,2550 0,1160 965,0571 21 0,9650 0,9800
0,139 0,2300 0,0910 752,3020 22 0,7523 0,9400
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C.2 Tabel kurva perbandingan aktivitas spesifik tiap fraksi
Sampel fraksi A1 A2 Rata2 (µg/mL enzim)
(mg/mL enzim)
Volume (mL)
Total (mg)
Blanko 0,192
EKE 0,276 0,286 0,281 175,789 0,175 940 165,242
AL96 0-30% 0,447 0,445 0,446 320,526 0,321 2 0,64105 30-50% 0,566 0,567 0,566 426,228 0,426 2 0,85246 50-70% 0,530 0,534 0,532 395,964 0,396 2 0,79193 70-90% 0,450 0,460 0,455 328,421 0,328 2 0,65684
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C.3 Tabel data kurva karakterisasi suhu fraksi 30-50% dan fraksi 50-70%
C.3.1 Tabel data kurva karakterisasi suhu fraksi 30-50%
Vol sampel : 0,025 mL
Vol total : 1,20 mL
Waktu inkubasi : 15 menit
Pers. Linier : y=0,0838x +0,0261
Mr PNP : 139
Suhu Kontrol A1 A2 Rata2 Sampel- Kontrol
(mmol/menit mg enzim)
(mmol/menit mg enzim)/ (mg/mL
enzim) 35 0,0740 0,1040 0,0990 0,1015 0,0275 0,0006 0,0015 45 0,0790 0,1230 0,1090 0,1160 0,0370 0,0049 0,0117 55 0,0950 0,0950 0,1630 0,1290 0,0340 0,0036 0,0084 65 0,1510 0,2090 0,2040 0,2065 0,0555 0,0134 0,0315 75 0,2280 0,1670 0,3650 0,2660 0,0380 0,0054 0,0127 85 0,180 0,2120 0,2170 0,2145 0,0345 0,0038 0,0090
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C.3.2 Tabel data kurva karakterisasi suhu fraksi 50-70%
Vol sampel : 0,025 mL
Vol total : 1,20 mL
Waktu inkubasi : 15 menit
Pers. Linier : y=0,0838x +0,0261
Mr PNP : 139
Suhu Kontrol A1 A2 Rata2 Sampel - Kontrol
(mmol/menit mg enzim)
(mmol/menit mg enzim) / (mg/mL enzim) 35 0,0740 0,1170 0,1250 0,1210 0,0470 0,0095 0,0241 45 0,0790 0,1330 0,1390 0,1360 0,0570 0,0141 0,0357 55 0,0950 0,1510 0,1420 0,1465 0,0515 0,0116 0,0293 65 0,1510 0,2170 0,2130 0,2150 0,0640 0,0173 0,0438 75 0,2280 0,3980 0,3450 0,3715 0,1435 0,0537 0,1357 85 0,1800 0,2170 0,2210 0,2190 0,0390 0,0059 0,0149
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C.4 Tabel data kurva karakterisasi pH fraksi 30-50% dan fraksi 50-70%
C.4.1 Tabel data kurva karakterisasi pH fraksi 30-50%
Vol sampel : 0,025 mL
Vol total : 1,20 mL
Waktu inkubasi : 15 menit
Pers. Linier : y=0,0838x +0,0261
Mr PNP : 139
pH Kontrol A1 A2 Rata2 Sampel - Kontrol
(mmol/menit mg enzim)
(mmol/menit mg enzim) / (mg/mL enzim) pH 6 0,0400 0,0650 0,0700 0,0675 0,0275 0,0006 0,0015 pH 7 0,0430 0,0750 0,0740 0,0745 0,0315 0,0024 0,0058 pH 8 0,1020 0,1390 0,1370 0,1380 0,0360 0,0045 0,0106 pH 9 0,0920 0,1480 0,1490 0,1485 0,0565 0,0139 0,0326 pH 10 0,1200 0,2490 0,1470 0,1980 0,0780 0,0237 0,0557 pH 11 0,1620 0,2010 0,2070 0,2040 0,0420 0,0072 0,0170 pH 12 0,1260 0,1680 0,1620 0,1650 0,0390 0,0059 0,0138