Curriculum Vitae
25. C-terminal polybasic domain of rac mediates isoform-specific regulation of
chemotaxis and NADPH –oxidase activity in murine neutrophils in vivo. Yamauchi, A., Marchal, C., Molitoris, J., Pech, N., Knaus, U., Towe, J., Atkinson, S.J. & M.C. Dinauer.
(2004). American Society for Cell Biology Annual Meeting Washington DC 2004
26. Gopalakrishnan, S., Hallett MA., Atkinson, S.J., and Marrs, J.A. Dysregulation of aPKC signaling pathway and tight junction assemply during ischemic renal epithelial cell injury. American Society for Cell Biology Annual Meeting Washington DC 2004
27. Silvia B. Campos, Melanie Hosford, Ruben M. Sandoval, Mark A. Hallett, Simon J.
Atkinson, Bruce A. Molitoris. Cytokine-Induced F-Actin Formation and Reorganization in MS-1 Endothelial Cells Involves RhoA Activation. American Society of Nephrology Annual Meeting, St. Louis, MO 2004.
28. Marie-Dominique Filippi, Haiming Xu, Jason Towe, Chad E. Harris, Kathleen Szczur, Lei Wang, Simon Atkinson, Yi Zheng, David A. Williams. The Rho GTPase CDC42 Regulator CDC42GAP Plays a Critical Role in Neutrophil Migration Via Podosome-Like Formation. American Society of Hematology Annual Meeting. San Diego 2004.
29. Chang ll Suh, Natalie Stull, Yoshito Fuji, Sergio Grinstein, Michael Yaffe, Simon Atkinson, Mary C. Dinauer. Role for p40phox in Fcγ-Receptor-Induced NADPH Oxidase Activation. Session Type: Oral Session. American Society for Hematology Annual Meeting. San Diego. 2004.
30 Simon J. Atkinson, NT Raye, GJ Rhodes and KJ Kelly. TLR4 and Rac2 contribute to renal injury in sepsis. American Society of Nephrology Annual Meeting, Philadelphia 2005.
31 Mark A. Hallett and S.J. Atkinson RhoB GTPase is up-regulated in the kidney following ischemia. American Society for Cell Biology Annual Meeting, San Francisco 2005.
32. Mark A. Hallett and Simon J. Atkinson. Role of mTOR and AMPK signaling in regulation of Rho GTPase activity. American Society for Nephrology Annual Meeting, San Diego. 2006.
33. Nicole T. Raye and Simon J. Atkinson. Neutrophil protease granule release is a major contributor to renal ischemia/reperfusion injury. American Society for Nephrology Annual Meeting, San Francisco. 2007.
34. Mark A. Hallett, Hao Zhang and Simon J. Atkinson. mTOR and AMPK Signaling Regulate RhoA Activity during Renal Ischemia. American Society for Cell Biology Annual Meeting, Washington DC. 2007.
35. Hao Zhang and Simon Atkinson. AMPK Signaling regulates RhoA activity during ATP depletion. American Society for Nephrology Annual Meeting, Philadelphia, 2008
36. Hao Zhang and Simon Atkinson. Regulation of RhoA activity in ischemia. American Society for Cell Biology Annual Meeting. San Francisco. 2008.
Present Position:
Professor of Biology, Indiana University-Purdue University Indianapolis (IUPUI), 1992- present
Adjunct Professor, Indiana University School of Medicine, Biochemistry and Molecular Biology Department, 1992-present
Education:
B.S. City College of New York, 1965
Ph.D. University of California (Berkeley), 1971 Professional Experience:
Post-Doctoral Fellow, University of Sheffield, 1971-1973
Post-Doctoral Fellow, State University of New York (Albany), 1973-1974 Post-Doctoral Fellow, University of California (Santa Cruz), 1974-1975 Assistant Professor of Biology, IUPUI, 1975-1979
Associate Professor of Biology, IUPUI, 1980-1992 Sabbatical Leave, Eli Lilly & Co., 1982-1983
Sabbatical Leave, IUSM, Biochemistry and Molecular Biology Department, 2006 Professional Societies:
American Association for the Advancement of Science American Society for Microbiology
Genetics Society of America
Grants:
NSF: $459,451 2006-2011 (PI, Robin Wright, Co-PI Martin Bard) Subcontract: $98, 181
Cold Adaptation in Yeast: the Role of ERAD Associated Degradation and Sterol Metabolism in Yeast
NIH: R01 GM62104; $893, 000, 2004-2009 The Yeast Ergosterol Pathway
Burroughs Wellcome Fund; $425,000 1999-2004
Characterization of New Target sites for Antifungal Intervention in the Candida albicans Ergosterol Pathway
The Yeast Ergosterol Pathway
NIH: PHS R01 AI38598-01; $ 706,500 1995-2000 Yeast Ergosterol pathway-New Antifungal Target Sites
Predoctoral fellowship from American Heart Association for Matthew Kennedy.(July 1998-June 2000) and Beth Skaggs (July 1994-June1996)
US Army; $218,836 1995-1999
Determining Antifungal Target Sites in the Sterol pathway of the Yeasts Candida and Saccharomyces
Johnson & Johnson Focused Giving Award; $150,000 1992-1994
Cloning of Yeast Sterol Genes for the Purpose of Identifying New Targets for Antifungal Drugs
NIH; PHS R15 AG11116-01; Small Instrumentation Grant $10887, 1992
NIH; PHS R15 GM 45959; $113,876, 1991-1994 Seeking the Yeast Sparking Sterol
American Cyanamid; $26,874, 1991-1992
Cloning the Yeast delta 14 Sterol Reductase Gene
NIH:PHS IR01 GM 43609; $304,000 (Jarrett & Bard, Co PIs) 1989-1992
Eli Lilly and Company; $19,800, 1988-1991
Physiological Analyses of Antibiotic Resistant Sterol Mutants of Yeast
NIH: PHS R15 GM37449; $59,137. 1986-1988
Cloning and Disruption of the Yeast Sterol Transmethylase Gene
Merck, Sharp & Dohme Institute for Therapeutic Research; $5,150, 1983-1986 Isolation of HMG-CoA Reductase Mutants in Yeast
American Heart Association, Grant-in-Aid; $7,500. 1978-1980 Isolation of HMG-CoA Reductase Mutants in Yeast
Squibb Institute for Medical Research; $1,500. 1978-1979
Physiological Analyses of Antibiotic Resistant Sterol Mutants of Yeast Internal Funding
Feedstocks for a Biorenewable Economy, 2010, $90000.
RSFG, Protein Interactions between Two Enzymes in Yeast Sterol Biosynthesis, 2009
$35000
Purdue Research Fund International Travel Grant, June 2007 $500
Purdue Research Foundation Award, 1992-1994 $20125
Cloning and disruption of the Yeast SCP2 Gene (Pre-doctoral Fellowship to Beth Skaggs)
IUPUI School of Science Interdepartmental Grant, 1986
Indiana University Biomedical Research Support Grant, 1975, 1986 IUPUI PDP Grants, 1981, 1982, 1983
Indiana University Research Operations Committee Grant 1978, 1983
Purdue University XL Grant, 1979, 1980
Isolation of Yeast HMG-CoA Reductase Mutants IUPUI Summer Faculty Fellowship
Grants to Students
Indiana Affiliate American Heart Association Pre-doctoral Fellowships Beth Arthington-Skaggs 1994-1996
Mathew Kennedy 1998-2000 Patents:
Bard, M. and Ingolia, T. D. Novel DNA for Expression of Delta-Aminolevulinic acid Synthetase and Related Method. No. 4,902,620, Feb. 20, 1990
Lai, MH, Kirsch, DR. and Bard, M. A DNA Sequence Encoding Sterol D14 Reductase No. 5,512,472, April 30, 1996
Lai, MH, Kirsch, DR. and Bard, M. A DNA Sequence Encoding Sterol D14 Reductase No. 5,525,496, June 11, 1996
Lai, MH, Kirsch, DR. and Bard, M. Sterol Δ14 Reductase Screen. No. 5,591,576, Jan. 7, 1997
Bard, M. Novel DNA Encoding Sterol Transmethylase. No. 6, 225,075. May 1, 2001
NIH Training Grant recipient-U.C. Berkeley IUPUI School of Science Research Award, 2004
Mycology Scholar Award, Burroughs Wellcome Fund, 1999-2004 Teaching Enhancement Recognition Award, IUPUI 1999
Consulting:
Vitamins Inc. 1982
Miller Brewing Company, 1999 Taro Pharmaceuticals, 2002
Integrated BioScience Solutions, LLC, 2008
Full Refereed Publications:
Bard, M., Biochemical and Genetic Aspects of Nystatin Resistance in Saccharomyces cerevisiae, J. Bacteriol. 111: 649-657, 1972.
Bard, M., Woods, R. A. and Haslam, J. M., Porphyrin Mutants of Saccharomyces cerevisiae. Correlated Lesions in Sterol and Fatty Acid Biosynthesis. Biochem.
Biophys. Res. Comm. 56: 324-330, 1974.
Woods, R. A., Bard, M., Jackson, I. E. and Drutz, D. J., Resistance to Polyene Antibiotics and Correlated Sterol Changes in Two Isolates of Candida tropicalis from a Patient with an Amphotericin B Resistant Funguria, J. Infect. Dis. 129: 53-58, 1974.
Woods, R. A., Bard, M., Molzahn, S. W. and Gardner, I. E., Investigations into the Biosynthesis of 24(28)-dehydroergosterol in Saccharomyces cerevisiae, Microbios. 10A: 73-80, 1974.
Barton, D. H. R., Corrie, J. E. T., Widdowson, D. A., Bard, M. and Woods, R. A., Biosynthesis of Terpenes and Steroids. Part IX. The Sterols of Some Mutant Yeasts and Their Relationship to the Biosynthesis of Ergosterol, J. Chem. Soc.
Perkins Trans. I. No. 11: 1326-1333, 1974.
Barton, D. H. R., Corrie, J. E. T., Widdowson, D. A., Bard, M., and Woods, R. A., Biosynthetic Implications of the Sterol Content of Ergosterol-Deficient Mutants of Yeast. J. Chem. Soc. 1: 30-31, 1974.
Barton, D. H. R., Gunatilaka, A. A. L., Jarman, T. R., Widdowson, D. A., Bard, M. and Woods, R. A., Biosynthesis of Terpenes and Steroids. Part X. The Sterols of Some Yeast Mutants Double Defective in Ergosterol Biosynthesis, J. Chem. Soc.
Perkins Trans. I. No. 1: 88-92, 1975.
645-654, 1977.
Bard, M., Wilson, K. J. and Thompson, R. M., Isolation of Sterol Mutants in
Chlamydomonas reinhardi: Chromatographic Analyses, Lipids 13: 533-539, 1978.
Bard, M., Lees, N. D. and Kleinhans, F. W., Differences in Crystal Violet Uptake and Cation Induced Death in Yeast Sterol Mutants, J. Bacteriol. 135: 1146-1148, 1978.
Kleinhans, F. W., Lees, N. D., Bard, M., Haak, R. A. and Woods, R. A., ESR
Determinations of Membrane Permeability in a Yeast Sterol Mutant, Chem. Phys.
Lipids 23: 143-154, 1979.
Lees, N. D. Bard, M., Kemple, M., Haak, R. A. and Kleinhans, F. W., Membrane Order in Yeast Sterol Mutants, Biochim Biophys. Acta. 553: 469-475, 1979.
Lees, N. D., Lofton, S. L., Woods, R. A. and Bard, M., The Effects of Varied Energy Source and Detergent on the Growth of Sterol Mutants of Saccharomyces cerevisiae, J. Gen. Microbiol. 118: 209-214, 1980.
Bard, M., Neuhauser, J. L. and Lees, N. D., Caffeine Resistance in Saccharomyces cerevisiae, J. Bacteriol. 141: 999-1002, 1980.
Downing, J. F., Burrows, L. S. and Bard, M., The Isolation of Two Mutants of
Saccharomyces cerevisiae which Demonstrate Increased Activity of 3-Hydroxy- 3-Methylglutaryl CoA Reductase, Biochem. Biophys. Res. Comm. 94: 974-979, 1980.
Bard, M. and Downing, J. F., Genetic and Biochemical Aspects of Yeast Sterol Regulation Involving 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase, J.
Gen. Microbiol. 125: 415-420, 1981.
Bard, M. and Ingolia, T. D., Plasmid Mediated Complementation of a Delta- Aminolevulinic Acid Requiring Yeast Mutant, Gene 28: 195-199, 1984.
Lees, N. D., Kemple, M. D., Barbuch, R. J., Smith, M. A. and Bard, M., Differences in Membrane Order Parameter and Antibiotic Sensitivity in Ergosterol-producing Strains of Saccharomyces cerevisiae, Biochim. et Biophys. Acta. 776: 105-112, 1984.
Bard, M., Lees, N. D., Barbuch, R. J., and Sanglard, D. Characterization of a
Cytochrome P450 Deficient Mutant of Candida albicans. Biochem. Biophys. Res.
Comm. 147: 794-800 1987.
Mevinolin Resistant Mutants of Saccharomyces cerevisiae. J. Gen Microbiol., 134: 1071-1078, 1988.
Bard, M., Albrecht, M. R., Gupta, N., Guynn, C. J. and Stillwell, W.. Geraniol Interferes with Membrane Functions in Strains of Candida and Saccharomyces. Lipids, 23:
534-538, 1988.
Lees, N.D., Kleinhans, F.W., Broughton, M.C., Pennington, D.E., Ricker, V.A. and Bard, M. Membrane Fluidity in a Cytochrome P450 - Deficient Mutant of Candida albicans. Steroids, 53: 567-578, 1989.
Gaber, R.F., Copple, D.H., Kennedy, B.K., Vidal, M. and Bard, M. ERG6 is Essential for Normal Membrane Function in Saccharomyces cerevisiae but is Not Required for the Formation of the Cell Cycle "Sparking Sterol". Mol. Cell. Biol. 9: 3447- 3456, 1989.
Lees, N.D., Broughton, M.C., Sanglard, D. and Bard, M. Azole Sensitivity and Hyphal Formation of the Cytochrome P450 Deficient Mutant of Candida albicans.
Antimicrob. Agents & Chemother. 34: 831-836, 1990.
Goss, T.A., Bard, M., and Jarrett, H.W. High Performance Affinity Chromatography of DNA. J. Chromatog. 508: 279-287, 1990.
Ashman, W.H., Barbuch, R.J., Ulbright, C.E., Jarrett, H.W. and Bard, M. Cloning and Disruption of the Yeast C-8 Sterol Isomerase Gene. Lipids 26: 628-632, 1991.
Arthington, B.A., Bennett, L.G., Skatrud, P.L., Guynn, C.J., Barbuch, R.J., Ulbright, C.E., and Bard, M. Cloning, Disruption, and Sequence of the Gene Encoding the Yeast C-5 Sterol Desaturase Gene. Gene 102: 39-44, 1991.
Broughton, M.C., Bard, M. and Lees, N.D. Polyene Resistance in Ergosterol-Producing Strains of Candida albicans. Mycoses 34: 75-83, 1991.
Goss, T.A., Bard, M. and Jarrett, H.W. High Performance Affinity Chromotography of RNA. J. Chromatog. 588: 157-164,1991.
Arthington, B.A., Hoskins, J.A., Skatrud, P.L. and Bard, M. Nucleotide Sequence of the Gene Encoding Yeast C-8 Sterol Isomerase. Gene 107: 173-174,1991.
Heidepriem, R. W., Livant, P. D., Parish, E. J., Barbuch, R. J., Broaddus, M. G., and Bard, M. A Simple Method for the Isolation of Zymosterol from a Sterol Mutant of Saccharomyces cerevisiae. J. Steroid Biochem. Mol. Biol. 43: 741-743, 1992.
Demethylase) Mutations in Saccharomyces cerevisiae and Candida albicans. Lipids 28: 963-967,1993.
Burden, R. S., James, C. S., Bailey, A. M., Keon, J. P. R., Croxen, R, Bard, M., and Hargreaves, J. A. Isolation and Characterization of the ERG2 Gene Encoding Δ8- Δ7 Sterol Isomerase, from the Maize Smut Pathogen, Ustilago maydis . Experim.
Mycol. 18: 87-92,1994.
Lai, M. L., Bard, M., Pierson, C. A., Alexander, J. F., Goebl, M., Carter, G. T., and Kirsch, D. R. The Identification of a Gene Family in the Saccharomyces cerevisiae Ergosterol Biosynthesis Pathway. Gene. 140: 41-49, 1994.
Lai, M. L., Bard, M., and Kirsch, D. R. Identifications of a New Ypt/Rab-like Monomeric G-Protein Sequence in Saccharomyces cerevisiae . Yeast. 10: 399-402, 1994.
Keon, J.P.R., James, C.S., Court, S., Baden-Daintree, C., Bailey,A.M., Burden, R.S., Bard, M., and Hargreaves, J. A. Isolation of the ERG2 Gene, Encoding Sterol Δ8 to Δ7 Isomerase, from the Rice Blast Fungus Magnaporthe grisea and its
Expression in the Maize Smut Pathogen, Ustilago maydis Curr. Genet. 25: 531- 537, 1994.
Bard, M., Bruner, D.A., Pierson, C.A., Lees, N.D., Biermann, B., Frye, L., Koegel, C., and Barbuch, R. Cloning and Characterization of ERG25, the S. cerevisiae Gene Encoding C-4 Sterol Methyl Oxidase. Proc. Natl. Acad. Sci. (USA). 93: 186-190, 1996.
Skaggs, B.A., Alexander, J.F., Pierson,C.A., Schweitzer, K.S., Chun,K.T., Koegel, C.
Barbuch, R. and Bard, M. Cloning and Characterization of the Saccharomyces cerevisiae C-22 Sterol Desaturase Gene Encoding a Second Cytochrome P450 Involved in Ergosterol Biosynthesis. Gene. 169: 105-109, 1996
Gachotte, D., Husselstein, T., Bard, M., Lacroute, F. and Benveniste, P. Isolation and Characterization of an A. thaliana cDNA Encoding a Δ7-sterol -C-5-desaturase by Functional Complementation of a Defective Yeast Mutant. Plant J. 9: 391-398, 1996.
Husselstein, T., Gachotte, D., Desprez, T., Bard, M., and Benveniste, P. Transformation of Saccharomyces cerevisiae with a cDNA Encoding a Sterol-C-methyl
Transferase from Arabidopsis thaliana Results in the Synthesis of 24-ethyl Sterols. FEBS Lett. 381: 87-92, 1996.
Process. Science. 272: 1353-1356, 1996.
Arthington-Skaggs, B.A., Crowell, D.N., Yang, H., Sturley, S.L., and Bard, M. Positive and Negative Regulation of a Sterol Biosynthetic Gene (ERG3) in the Post-
Squalene Portion of the Yeast Ergosterol Pathway. FEBS Lett. 392: 161-165. 1996.
Gachotte, D., Pierson, C.A., Lees, N.D., Barbuch, R., Koegl, C., and Bard, M. A Yeast Sterol Auxotroph (erg25) is Rescued by Addition of Azole Antifungals and Reduced Levels of Heme. Proc. Natl. Acad. Sci. (USA). 94: 11173-11178, 1997.
Jensen-Pergakes, K.L., Kennedy, M.A., Lees, N.D., Barbuch,R., Koegel, C., and Bard, M. Sequencing, Disruption, and Characterization of the Candida albicans Sterol methyltrasferase (ERG6) Gene: Drug Susceptibillity Studies in erg6 Mutants.
Antimicrobial. Agents Chemother. 42: 1160-1167, 1998.
Gachotte, D., Barbuch, R., Gaylor, J., Nickel, E. annd Bard, M. Characterization of the Saccharomyces cerevisiae ERG26 Gene Encoding the C-3 Sterol Dehydrogenase (C-4 decarboxylase) Involved in Sterol Biosynthesis. Proc. Natl. Acad. Sci.
(USA) 95: 13794-13799, 1998.
Kennedy, M.A., Barbuch, R., and Bard, M. Transcriptional Regulation of the Squalene Synthase Gene (ERG9) in the Yeast Saccharomyces cerevisiae . Biochem.
Biophys. Acta 1445: 110-122, 1999.
Gachotte, D., Sen, S.E., Eckstein, J., Barbuch, R., Krieger, M., Ray, B.D., and Bard, M.
Characterization of the Saccharomyces cerevisiae ERG27 Gene Encoding the 3- keto Reductase Involved in C-4 Sterol Demethylation. Proc. Natl. Acad. Sci.
(USA) 96: 12655-12660, 1999.
Kennedy, M.A., Johnson, T.A., Lees, N.D., Barbuch, R., Eckstein, J.A., and Bard, M.
Cloning and Sequencing of the Candida albicans C-4 Sterol Methyl Oxidase Gene (ERG25) and Expression of an ERG25 Conditional Lethal Mutation in
Saccharomyces cerevisiae. Lipids 35: 257-262, 2000.
Li, X., Routt, S.M., Xie, Z., Cui, X., Fang, M., Kearns, M.A., Bard, M., Kirsch, D.A., and Bankaitis, V.A. Idenification of a Novel Family of Nonclassic Yeast Phosphatidyl Transfer Proteins Whose Function Modulates Phospholipase D Activity and Sec14p-independent Cell Growth. Mol. Biol. Cell 11: 1989-2005, 2000.
Hughes, T.R., Marton, M.J., Jones, A.R., Roberts, C.J., Stoughton, R., Armour, C.D., Bennett, H.A., Coffey, E., Dai, H., He, Y.D., Kidd, M.J., King, A.M., Meyer, M.R., Slade, D., Lum, P.Y., Stepaniants, S.B., Shoemaker, D.D., Gachotte, D.,
Tinkelenberg, A.H., Liu, Y., Alcantara, F., Khan, S., Guo, Z., Bard, M. and Sturley, S.L.
Mutations in Yeast ARV1 Alter Intracellular Distribution and Are Complemented by Human ARV1. J. Biol. Chem. 275: 40667-40670, 2000.
Kennedy, M.A. and Bard, M. Positive and Negative Regulation of Squalene Synthase (ERG9) an Ergosterol Biosynthetic Gene in Saccharomyces cerevisiae. Biochim.
Biophys. Acta. 1517: 177-189, 2001.
Gachotte, D., Eckstein, J., Barbuch, R., Hughes, T., Roberts, C. and Bard, M. A Novel Gene Conserved from Yeast to Humans is Involved in Sterol Biosynthesis. J.
Lipid Res. 42: 150-154, 2001.
Aaron, K.A., Pierson, C.A., Lees, N.D., and Bard, M. The Candida albicans ERG26 Gene Encoding the C-3 Sterol Dehydrogenase (C-4 decarboxylase) is Essential for Growth. FEMS Yeast Res. 1: 93-101, 2001.
Jensen-Pergakes, K., Guo, Z., Giattina, M., Sturley, S.L. and Bard, M. Transcriptional Regulation of the Two Sterol Esterification Genes in the Yeast Saccharomyces cerevisiae. J. Bacteriol. 183: 4950-4957, 2001.
Darnet, S. Bard, M. and Rahier, A. Functional Identification of Sterol-4α-Methyl
Oxidase cDNAs from Arabidopsis thaliana by Complementation of a Yeast erg25 Mutant Lacking Sterol-4α-Methyl Oxidation. FEBS Lett. 508: 39-43, 2001.
Jia, N., Arthington-Skaggs, B., Lee, W., Pierson, C.A., Lees, N.D., Eckstein, J., Barbuch, R., and Bard, M. Candida albicans Sterol C-14 Reductase, Encoded by the
ERG24 Gene, as a Potential Antifungal Target Site. Antimicrobial. Agents Chemother. 46: 947-957, 2002.
Mo, C., Valachovic, M., Randall, S.K., Nickels, J.T., and Bard, M. Protein-Protein Interactions Among C-4 Demethylation Enzymes Involved in Yeast Sterol Biosynthesis. Proc. Natl. Acad. Sci.(USA) 99: 9739-9744, 2002.
Hongay, C., Jia, N., Bard, M. and Winston, F. Mot3 Is a Transcriptional Repressor of Ergosterol Biosynthetic Genes and Is Required for Normal Vacuolar Function in Saccharomyces cerevisiae. EMBO J. 21: 4114-4124, 2002
Hand, R. A., Jia, N., Bard, M., and Craven, R.J. Saccharomyces cerevisiae Dap1p, a Novel DNA Damage Response Protein Related to the Mammalian Membrane Associated Progesterone Receptor. Euk. Cell 2: 306-317, 2003.
Mo, C., Milla, P., Athenstaedt, K., Ott, R., Balliano, G., Daum, G. and Bard, M. In Yeast Sterol Biosynthesis the 3-Keto Reductase Protein (Erg27p) is Required
Lucas, M.E., Ma, Q., Cunningham, D., Peters, J., Cattanach, B., Bard, M., Elmore, B.K., and Herman, G.E. Identification of Two Novel Mutations in the Murine Nsdh1 Sterol Dehydrogenase Gene and Development of a Functional
Complementation Assay in Yeast. Mol. Genet. Metabol. 80: 227-233, 2003.
Lum, Y.L., Armour, C.D., Stepaiants, S.B., Cavet, G., Wolf, M.K., Butler, J.S., Hinshaw, J.C., Garnier, P., Prestwich, G.D., Leonardson, A., Garrett-Engele, P., Rush, C. M., Bard, M., Schimmack, G., Phillips, J.W., Roberts, C.J., and Shoemaker, D. D. Discovering Modes of Action for Therapeutic Compounds using a Genome-Wide Screen of Yeast Heterozygotes. Cell 116:121-137, 2004
Pierson, C.A. Eckstein, J., Barbuch, R., and Bard, M. Ergosterol Gene Expression in Wildtype and Ergosterol -Deficient Mutants of Candida albcians. Med.
Mycol. 42:385-389, 2004
Tsai, H., Bard, M., Izumikawa, K., Krol, A.A., Sturm, A.M., Culbertson, N.T., Pierson, C.A., and Bennett, J. A Candida Glabrata ERG1 Mutant with Increased Sensitivity to Azoles and to Low Oxygen Tension. Antimicrob. Agents Chemother. 48:2483-2489, 2004
Chen, O.S. Crisp, R. J., Valachovic, M., Bard, M. Winge, D.M. and Kaplan, J.
Transcription of the Yeast Iron Regulon Responds Not Directly to Iron but Rather to Iron-sulfur Cluster Biosynthesis. J. Biol. Chem. 279:29513-29518, 2004
Rogers, K.M., Pierson, C.A., Culbertson, N.T., Mo, C., Sturm, A.M., Eckstein, J., Barbuch, R., Lees, N.D., and Bard, M. Disruption of the Candida albicans CYB5 Gene Results in Increased Azole Sensitivity. Antimicrob. Agents Chemother.
48:3425-3435, 2004.
Barker, K.S., Crisp, S., Wiederhold, N. Lewis, R.E., Bareither, B., Eckstein, J., Barbuch, R., Bard, M. and Rogers, P.D. Genome-wide Expression Profiling Reveals Genes Associated with Amphotericin B and Fluconazole Resistance in Experimentally Induced Antifungal Resistant Isolates of Candida albicans. J. Antimicrob.
Chemother. 54:376-385, 2004
Pierson, C.A., Jia, N., Mo, C., Lees, N.D., Sturm, A.M., Eckstein, J., Barbuch, R., and Bard, M. Isolation, Characterization, and Regulation of the Candida albicans ERG27 Gene Encoding the Sterol 3-Keto Reductase. Med. Mycol. 42:461-473, 2004
Mo, C., Valachovic, M., and Bard, M. The ERG28 Encoded Protein, Erg28p, Interacts with Both the Sterol C-4 Demethylation Enzyme Complex as Well as the Late
Valachovic, N., Wilcox, L. J., Sturley, S.L., and Bard, M. A Mutation in Sphingolipid Synthesis Suppresses Defects in Yeast Ergosterol Metabolism. Lipids 39: 747- 752, 2004
Mallory, J.C., Crudden, G., Johnson, B.L., Mo, C. Pierson, C.A., Bard, M. and Craven, R.J. Dap1p, a Heme-Binding Protein That Regulates the Cytochrome P450 Protein Erg11p/Cyp51p in Saccharomyces cerevisiae. Mol. Cell. Biol. 25: 1669- 1679, 2005
Bard, M., Sturm, A.M., Pierson, C.A., Brown, S., Rogers, K.M., Nabinger, S. Eckstein, J., Barbuch, R., Lees, N. D., Howell, S.A. and Hazen, K.C. Sterol Uptake in Candida glabrata: Resuce of Sterol Auxotrophic Mutants. Diagn. Microbiol.
Infec. Dis. 52:285-293,2005
Mo, C. and Bard, M. Erg28p is a Key Protein in the Yeast Sterol Biosynthetic Enzyme Complex. J. Lipid Res. 46:1991-1998, 2005
Mo, C. and Bard, M. A Systematic Study of Yeast Sterol Biosynthetic Protein-protein Interactions Using the Split-Ubiquitin System, Biochim. Biophys. Acta 1737:152- 160, 2005
Valachovic, M., Bareither, B. M., Bhuiyan, M. S. A., Eckstein, J. Barbuch, R., Balderes, D., Wilcox, L., Sturley, S. L., Dickson, R. C. and Bard, M. Cumulative Mutations Affecting Sterol Biosynthesis In The Yeast Saccharomyces cerevisiae Result In Synthetic Lethality That Is Suppressed By Alterations In Sphingolipid Profiles.
Genetics 173: 1893-1908, 2006
Loertscher, J., Larson, L.L, Matson, C.K., Parrish, M.L., Felthauser, A., Sturm, A., Tachibana, T., Bard, M. and Wright, R. Endoplasmic Reticulum Associated Degradation Is Required for Cold Adaptation and Regulation of Sterol
Biosynthesis in the Yeast Saccharomyces cerevisiae, Euk. Cell 5:712-722, 2006.
Rahier A, Darnet S, Bouvier F, Camara B, and Bard M. Molecular and enzymatic characterizations of novel bifunctional 3beta -hydroxysteroid-dehydrogenases/C4- decarboxylases from Arabidopsis thaliana. J. Biol. Chem. 281: 27264-27277, 2006
Bhuiyan, M.S.A., Eckstein, J., Barbuch, R., and Bard, M. Synthetic Lethal Interactions Involving Loss of the Yeast ERG24-the Sterol C-14 Reductase Gene. Lipids 42:69-76, 2007
Enzymes. Cell Metab. 5:143-149, 2007
Nakayama, H., Tanabe, K., Bard, M., Hodgson, W., Wu, S., Takemori, D., Aoyama, T., Kumaraswami, N., Metzler, L., Takano, Y., Chibana, H., and Niimi, M. The Candida glabrata Putative Sterol Transporter Gene CgAUS1 Protects Cells Against Azoles in The Presence Of Serum. J. Antimicrobiol. Chemother.
60:1264-1272, 2007
Teske, B., Taramino, S., Bhuiyan, M.S.A., Kumaraswami, N. S., Randall, S.K., Barbuch, R., Eckstein, J., Balliano, G., and Bard, M. Genetic Analyses Involving
Interactions Between the Ergosterol Biosynthetic Enzymes, Lanosterol Synthase (Erg7p) and 3-Ketoreductase (Erg27p) in the Yeast Saccharomyces cerevisiae.
Biochim. Biophys. Acta. 1781:359-366, 2008
Williger, S.D., Puttikamonkul, S., Kim, K.-H., Burrtitt, J.B., Grahl, N., Metzler, L.J., Barbuch, R., Bard, M. Lawrence, C.B. and Cramer, R.A. A Sterol Regulatory Element Binding Protein Is Required for Cell Polarity, Hypoxia, Adaptation, Azole Drug Resistance, and Virulence in Aspergillus fumigatus. PLoS Pathog 4(11) 1-18 e1000200, 2008
Taramino, S., Valachovic, M., Oliaro-Bosso, S., Viola, F., Teske, B., Bard, M., and Balliano, G. Interactions of Oxidosqualene Cyclase (Erg7p) with 3-
Ketoreductase (Erg27p) and Other Enzymes of Sterol Biosynthesis in Yeast.
Biochim. Biophys. Acta. 1801:156-162, 2010
Shakoury-Elizeh, M., Protchenko, O., Berger, A., Cox, J., Gable, K., Dunn,T.M., Prinz, W.A., Bard, M., and Philpott, C.C The Metabolic Response To Iron Deficiency In Saccharomyces cerevisiae. J. Biol. Chem. 285: 14823-14833, 2010
Taramino, S., Teske, B., Oliaro-Bosso, S., Bard, M. and Balliano, G. Divergent Interactions Involving the Oxidosqualene Cyclase and 3-Ketoreductase in the Sterol Biosynthetic Pathways of Mammals and Yeasts Biochim. Biophys. Acta.
1801:1232-1237, 2010
Nagi, M., Nakayama, H.,Tanabe, K., Bard, M., Aoyama, T., Okano, M., Higashi, S., Ueno, K., Chibana, H., Niimi, M., Yamagoe, S., Umeyama, T., Kajiwara, S., Ohno, H., and Miyazaki, Y. Transcription Factors CgUPC2A and CgUPC2B Regulate Ergosterol Biosynthetic Genes in Candida glabrata. Genes Cells, In Press
Invited Book Chapters and Review Articles
cerevisiae. p. 246-259. ACS Symposium Series "Regulation of Isopentenoid Metabolism". W. D. Nes, E. J. Parish, and J. M. Trzaskos eds. American Chemical Society, Washington, D.C. ,1992
Lees, N.D, Skaggs, B., Kirsch, D.R., and Bard, M. Cloning of the Later Genes in the Ergosterol Biosynthetic Pathway of Saccharomyces cerevisiae-A Review. Lipids 30: 221-226, 1995.
Lees, N. D., Bard, M., and Kirsch, D. Biochemistry and Molecular Biology of Sterol Synthesis in Saccharomyces cerevisiae In Biochemistry and Function of Sterols (Parish, E.J. and Nes, W.D. editors) CRC Press. chap.16 pp. 85-100, 1997
Lees, N. D., Bard, M., and Kirsch, D. Biochemistry and Molecular Biology of Sterol Synthesis in Saccharomyces cerevisiae In Biochemistry and Function of Sterols Crit Rev Biochem Mol Biol. 34(1): 33-47, 1999.
Daum, G., Lees, N.D., Bard, M., and Dickson, R. Biochemistry, Cell Biology, and Molecualr Biology of Lipids of Saccharomyces cerevisiae Yeast 14: 1471-1510, 1998.
Lees, N.D. and Bard, M. Sterol Biochemistry and Regulation in the Yeast Saccharomyces cerevisiae. In Topics in Current Genetics 6: 213-240 (G. Daum, ed.) Springer- Verlag Heidelberg, 2003
Abstracts/Presentations/Posters:
Woods, R. A., Bard, M., Jackson, I. E. and Drutz, D. J., Resistance to Polyene Antibiotics and Correlated Sterol Changes in Clinical Isolates of Candida tropicalis, J. Gen.
Microbiol. 77: iii, 1973.
Bard, M., Woods, R. A. and Haslam, J. M., Correlation between Defects in the Synthesis of Sterols, Unsaturated Fatty Acids, Methionine and Porphyrins in the ole
Mutants of Yeast. Heredity 32: 283, 1974.
Kleinhans, F. W., Bard, M., Lees, N. D. and Haak, R. A., Increased Permeability of Yeast Membrane Mutants to Ni++ as Detected by ESR, Bull. Amer. Phys. Soc. 22: 277, 1977.
Kleinhans, F. W., Lees, N. D., Bard, M. and Haak, R. A., ESR Determination of Membrane Order in Yeast Sterol Mutants. Biophys J. 21: 36a, 1978.
Bard, M., Wilson, K. and Thompson, R. M., Isolation of Sterol Mutants in
Chlamydomonas reinhardi, Chromatographic analysis, Amer. J. Bot. 65S, 1978.