Chapter 1: Introduction
1.5 Thesis overview
In this thesis, I explore novel microfluidic fabrication techniques that utilize 3D printing, the use of hydrogel materials, and numerical simulations to guide microbial analytical device design. The roadmap of this thesis is shown in Figure 1.4.
Chapter 2 describes the use of multiphysics simulation in the development of several novel 3D microfluidic devices for nucleic acid-based pathogen detection in environmental water samples. The developed simulation models aided the design of a microfluidic chip for live versus dead cell differentiation, the understanding of DNA extraction performance in a milliliter-chamber device for electrochemical cell lysis, and the validation of cell distribution on an asymmetric membrane for integrated digital detection.
Chapter 3 describes the development of a hydrogel bead-based (Gelbead) platform that links single cell phenotypic and molecular analysis. In this chapter, I describe how needles and microcentrifuge tubes were adapted into 3D microfluidic devices for monodispersed droplet generation. The disposable device enabled the combination of fast- crosslinking biocompatible hydrogel with droplet microfluidics. The hydrogel bead-based Figure 1.4 Roadmap of this dissertation.
assays were established for cell viability assessment, digital PCR, digital LAMP, and in situ PCR, following cell viability assessment.
Chapter 4 describes the ongoing work of developing a bead sorter that is a portable and affordable solution for benchtop and potentially point-of-care bead analysis and sorting.
The hydrogel beads are interrogated individually, and are sorted by acoustophoretic force based on the fluorescence intensity for downstream analysis such as PCR or sequencing. The sorter system has been designed and prototyped, and its feasibility was studied through COMSOL simulation.
Chapter 5 describes the provisional work based on the development reported in previous chapters. The proposed work includes the development of DropTube, which is a fully integrated centrifugal device for various in-field pathogen analysis, and the investigation of AMR evolution kinetics through the Gelbead platform.
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C h a p t e r 2
CHAPTER 2: MULTIPHYSICS SIMULATION IN