ATOM INDONESIA
Editor’s Report
Article No. : #535
Title of Paper :
Fungal Population and Aflatoxin Contamination on Stored Gamma Irradiated Nutmeg (Myristica fragrans) Kernels
Referee Name : Darmawan Darwis
Comment on Descriptions 1. Title
[ X ] Appropriate [ ] Should be changed 2. Abstract
Yes[ X ] No[ ] Is the length reasonable?
Yes[ ] No[ ] Is it an appropriate summary of the content?
3. Main Text
Yes[X ] No[ ] Is there anything new in this work?
Yes[ X ] No[ ] Is the relation to previous studies adequately stated?
Yes[ X ] No[ ] Are the assumption(s) and/or method(s) described comprehensively?
Yes[ X ] No[ ] Are the new results adequately emphasized?
Line # Referee’s Comments
Please see the suggestion of reviewer in manuscript. Please revised or corrected it according to reviewers suggestion. .
17
Abstract:
Irradiation Change Irradiation dose however, 5 species change
however, 5 strains
48
that can be used corrected:
that can be used to kill or to reduce microbial contamination and 74-75 How do you know that the seeds has been fall one week before collecting?
76-80 See the reviewers suggestion in manuscript
85-91 See the reviewers suggestion in manuscript
91-93
Written: The Center for Application of Isotope and Radiation Technology, National Nuclear Energy Agency
Change:
The Center for Isotope and Radiation Application, National Nuclear Energy Agency
100- 109
1. Please re-write in more detail the method how to prepare sample before and after irradiation, number of bags sample for each irradiation doses in order to make understand that the samples especially for samples stored for 2, and 4 months were not opened before used for parameter analysis to prevent recontamination after irradiation.
2. Are you grind the kernels just after irradiation for sample storage 2, and 4 months? If so, the recontamination of microbes probably occurred.
3.Let say that you have 2 irradiation doses (5 and 10 kGy) and one control and 3 storage duration (0, 2, and 4 months), and 3 replications. So you should have 27 samples.
Comment [sv1]: Strains
Comment [sv2]: Application (CIRA)
207 what does it mean?
213 Please explain why the population of C. Cladosporioides and P. Citrinum increase after stored for 4 months for samples irradiated at 10 kGy. While irradiation kill all microbes (0 population) 213-
215
Your explanation is not matching with the data in Table 1.
Look at the table, at 5 kGy and 10 kGy doses, (at 0 month storage duration), all microbes are killed excepted for A. flavus. This indicated that radiation is effective to kill A. niger, C. cladospioides, E.
chevalieri and P. citrinum.
222- 228
These data show that irradiation at 10 kGy is effective to kill all microbes (bacteria and fungi) except A. flavus
321 Need correction. See comment
Final comments and recommendations:
In general, the article contains a lot of information on the application of gamma irradiation for decontamination of bacteria and fungi. However, it needs clarification especially in methods and some correction needed according to reviewer suggestions. It can be accepted for J AI after correction.
Please see the suggestion of reviewer in manuscript. Please revised or corrected it according to reviewers suggestion.
The reviewer needs to check again the revised manuscript by the author
This paper is recommended to be [ ] Accepted without further revision [ ] Accepted with minor revision [ x ] Major Revision is required [ ] Rejected
