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DAFTAR PUSTAKA

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58. Wilsher S, Newcombe JR, Allen WR (Twink). The immunolocalization of Galectin-1 and Progesterone-Induced Blocking Factor (PIBF) in equine trophoblast: Possible roles in trophoblast invasion and the immunological protection of pregnancy. Placenta. 2019;85(6):32–9.

59. Hudić I, Stray-Pedersen B, Szekeres-Bartho J, Fatušić Z, Dizdarević- Hudić L, Tomić V, et al. Maternal serum progesterone-induced blocking factor (PIBF) in the prediction of preterm birth. J Reprod Immunol.

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88

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Lampiran 1. Kuesioner penelitian

DATA PASIEN Waktu pemeriksaan :

Tempat pengambilan data : Nomer sampel :

IDENTITAS :

Nama / No.CM :

Tempat , tanggal lahir : Pendidikan : SD/ SMP/ SMA/ S1/ S2

Agama : Pekerjaan : Tidak Bekerja/ PNS/ Swasta

Alamat :

No Telp/ HP :

Penghasilan : 1. < Rp.1.000.000

2. Rp. 1.000.000 – Rp. 2.000.000 3. Rp. 2.000.000 – Rp. 5.000.000 4. > Rp. 5.000.000

ANAMNESIS :

Keluhan :

HPHT :

Riw. Nikah :

Riw. Obstetri : G P A 1.

2.

3.

4.

Penggunaan obat progesteron : +/-

Riw. Penyakit Dahulu : Preeklampsia kehamilan sebelumnya : -/+

Diabetes melitus : -/+ Asma : -/+ Hipertensi : -/+

Penyakit tiroid : -/+ Infeksi kronis (sepsis) : -/+

Keganasan : -/+ Riwayat operasi +/- PEMERIKSAAN FISIK :

TD : HR : RR : T :

BB : TB : BMI :

KEPALA :

LEHER :

THORAK :

ABDOMEN :

EKSTERMITAS :

PEMERIKSAAN GINEKOLOGI Inspekulo /VT : Fluksus ( )/fluor ( ) Vulva/ uretra/ Vagina :

Portio :

Corpus Uterus : Adnexa parametrium : Cavum douglas :

PEMERIKSAN PENUNJANG :

USG :

Hasil Lab :

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90 Lampiran 1. Kuesioner penelitian

KUESIONER

No. Pertanyaan Ya Tidak

1. Apakah anda hamil dengan usia <20 tahun atau >35 tahun?

2. Apakah ini hamil yang pertama?

3. Apakah ini hamil yang keempat atau lebih?

4. Apakah saat ini usia kehamilan anda <14 minggu?

5. Apakah anda memiliki kebiasaan aktivitas/pekerjaan yang berat?

6. Apakah anda memiliki riwayat jatuh/terbentur benda keras?

7. Apakah selama hamil anda merasa stress berlebih?

8. Apakah anda memiliki penyakit gula, tekanan darah tinggi kronis, gagal ginjal kronis, infeksi kronis, penyakit tiroid, penyakit serebrovaskuler sebelum kehamilan?

9. Apakah saat ini mengalami keadaan ancaman kematian janin dalam rahim?

10. Apakah sebelumnya anda pernah mengalami keadaan kematian janin dalam rahim?

Apakah anda memiliki riwayat penyakit genetik/keturunan?

11. Apakah suami anda memiliki riwayat penyakit genetik/keturunan?

12. Apakah suami anda memiliki riwayat penyakit anemia?

13. Apakah anda memiliki riwayat kelainan kongenital pada rahim?

14. Apakah anda memiliki riwayat kelemahan otot leher rahim?

15. Apakah anda memiliki riwayat keganasan organ genitalia?

15. Apakah anda memiliki riwayat penyakit polip serviks?

16. Apakah anda memiliki riwayat preeklampsia sebelumnya?

17. Apakah selama hamil anda rutin mengonsumsi suplemen seperti tablet tambah darah maupun kalsium?

18. Apakah anda menerima terapi progesteron selama kehamilan?

19. Apakah anda memiliki riwayat merokok, minum alkohol?

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Lampiran 2. Ethical clearance

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92 Lampiran 3. Izin penelitian

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Lampiran 3. Izin penelitian

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94 Lampiran 4. Statistik penelitian

Descriptives

Kelompok Statistic Std. Error

Usia Abortus Iminens Mean

95% Confidence Interval

for Mean Lower Bound

Upper Bound 5% Trimmed Mean

Median Variance Std. Deviation Minimum Maximum Range

Interquartile Range Skewness Kurtosis

Kontrol Mean

95% Confidence Interval for Mean

Lower Bound Upper Bound 5% Trimmed Mean

Median Variance Std. Deviation Minimum Maximum Range

Interquartile Range Skewness Kurtosis PIBF Abortus Iminens Mean

95% Confidence Interval

for Mean Lower Bound

Upper Bound 5% Trimmed Mean

Median Variance Std. Deviation Minimum Maximum Range

Interquartile Range Skewness Kurtosis

Kontrol Mean

31.50 2.542 25.49

37.51 31.39 30.50 51.714 7.191 2 1 4 4 2 3 9

.387 .752

.018 1.481 34.75 3.750 22.82

46.68 34.89 36.00 56.250 7.500 2 5 4 2 1 7 1 4

- . 7 5 0 1.014 - . 7 1 5 2.619 69.1575 1.86179 64.7551

73.5599 69.2106 68.5700 27.730 5.26593 61.54 75.82 14.28 9.48

- . 0 0 3 .752 - 1 . 5 5 1 1.481 101.5225 7.33560

78.1773

Page 3

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Descriptives

Kelompok Statistic Std. Error

PIBF

Kontrol

95% Confidence Interval

for Mean Lower Bound

Upper Bound 5% Trimmed Mean

Median Variance Std. Deviation Minimum Maximum Range

Interquartile Range Skewness Kurtosis

78.1773 124.8677 102.1372 107.0550 215.244 14.67120 79.88 112.10 32.22 24.89

- 1 . 8 0 4 1.014

3.377 2.619

Tests of Normality

Kelompok

Kolmogorov-Smirnova Shapiro-Wilk

Statistic d f Sig. Statistic d f Sig.

Usia Abortus Iminens Kontrol PIBF Abortus Iminens

Kontrol

.187 8 .200* .958 8 .790

.215 4 . .953 4 .735

.217 8 .200* .911 8 .362

.359 4 . .793 4 .090

This is a lower bound of the true significance.

*.

Lilliefors Significance Correction a.

Usia

Normal Q-Q Plots

Page 4

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96

Descriptives

Kelompok Statistic Std. Error

PIBF Abortus Iminens

Variance Std. Deviation Minimum Maximum Range

Interquartile Range Skewness Kurtosis

Kontrol Mean

95% Confidence Interval

for Mean Lower Bound

Upper Bound 5% Trimmed Mean

Median Variance Std. Deviation Minimum Maximum Range

Interquartile Range Skewness Kurtosis

91.131 9.54625 53.00 69.85 16.85 .

1.723 1.225

. .

95.5867 7.69582 62.4742

128.6991 . 98.7500 177.677 13.32955 80.96 107.05 26.09 .

- 1 . 0 0 8 1.225

. .

Tests of Normality

Kelompok

Kolmogorov-Smirnova Shapiro-Wilk

Statistic d f Sig. Statistic d f Sig.

Usia Abortus Iminens Kontrol PIBF Abortus Iminens

Kontrol

.385 3 . .750 3 .000

.175 3 . 1.000 3 1.000

.373 3 . .779 3 .065

.260 3 . .958 3 .605

Lilliefors Significance Correction a.

Usia

Normal Q-Q Plots

Page 1 1

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Independent Samples Test Levene's Test for Equality of

Variances

t-test for Equality of Means

F Sig. t d f

Usia Equal variances assumed Equal variances not assumed

PIBF Equal variances assumed Equal variances not assumed

.000 1.000 - . 7 2 8 1 0 .483

- . 7 1 7 5.861 .501

5.073 .048 - 5 . 7 6 7 1 0 .000

- 4 . 2 7 6 3.393 .018

Independent Samples Test

t-test for Equality of Means

Sig. (2-tailed)

Mean Difference

Std. Error Difference

95%

Confidence ...

Lower Usia Equal variances assumed

Equal variances not assumed

PIBF Equal variances assumed Equal variances not assumed

.483 - 3 . 2 5 0 4.461 - 1 3 . 1 9 0 6.690

.501 - 3 . 2 5 0 4.531 - 1 4 . 4 0 0 7.900

.000 - 3 2 . 3 6 5 0 0 5.61196 - 4 4 . 8 6 9 2 3 - 1 9 . 8 6 0 7 7 .018 - 3 2 . 3 6 5 0 0 7.56818 - 5 4 . 9 4 6 8 1 - 9 . 7 8 3 1 9

Independent Samples Test t-test for

Equality of ...

95% Confidence Interval of the ...

Upper Usia Equal variances assumed

Equal variances not assumed

PIBF Equal variances assumed Equal variances not assumed

6.690 7.900 - 1 9 . 8 6 0 7 7 - 9 . 7 8 3 1 9

USE ALL.

COMPUTE filter_$=(Usia_Gestasi = 8).

VARIABLE LABELS filter_$ 'Usia_Gestasi = 8 (FILTER)'.

VALUE LABELS filter_$ 0 'Not Selected' 1 'Selected'.

Page 4 4

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98

Case Processing Summary

Kelompok

Cases

Valid Missing Total

N Percent N Percent N Percent

Usia Abortus Iminens Kontrol PIBF Abortus Iminens

Kontrol

3 100.0% 0 0.0% 3 100.0%

3 100.0% 0 0.0% 3 100.0%

3 100.0% 0 0.0% 3 100.0%

3 100.0% 0 0.0% 3 100.0%

Descriptives

Kelompok Statistic Std. Error

Usia Abortus Iminens Mean

95% Confidence Interval

for Mean Lower Bound

Upper Bound 5% Trimmed Mean

Median Variance Std. Deviation Minimum Maximum Range

Interquartile Range Skewness Kurtosis

Kontrol Mean

95% Confidence Interval for Mean

Lower Bound Upper Bound 5% Trimmed Mean

Median Variance Std. Deviation Minimum Maximum Range

Interquartile Range Skewness Kurtosis PIBF Abortus Iminens Mean

95% Confidence Interval

for Mean Lower Bound

Upper Bound 5% Trimmed Mean

Median

36.33 .667

33.46 39.20 . 37.00 1.333 1.155 3 5 3 7 2 .

- 1 . 7 3 2 1.225

. .

28.00 1.155 23.03

32.97 . 28.00 4.000 2.000 2 6 3 0 4 .

.000 1.225

. .

58.8333 5.51153 35.1191

82.5475 . 53.6500 91.131

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Lampiran 5. Hasil pengukuran PIBF

KEMENTERIAN PENDIDIKAN, KEBUDAYAAN, RISET, DAN TEKNOLOGI

UNIVERSITAS DIPONEGORO FAKULTAS KEDOKTERAN LABORATORIUM GAKI

Jalan Prof. Sudarto, S.H.

(Laboratorium Sentral FK Lantai 2) Tembalang Semarang Kode Pos 50275 Tel./Faks. (024) 76928010 www.fk.undip.ac.id│email:dean[at]fk.undip.ac.id

HASIL PEMERIKSAAN PIBF

“ Perbedaan Rerata Kadar Serum Progesteron-Induced Blocking Factor (PIBF) Abortus Imminens dengan Kehamilan Normal (Studi pada Kehamilan Trimester I) “

Peneliti : dr. Muhammad Reza Hidayat (PPDS I Obstetri – Ginekologi)

NO. NAMA HASIL PIBF

(ng/ml)

1. Ny. Uwaliyah 75,82

2. Ny. Maryatun 53,11

3. Ny. Anita Herawati 47,95

4. Ny. Nanik Rahayu 64,95

5. Ny. Siti Handayani 53,11

6. Ny. Mia Anggraini 48,70

7. Ny. Taslimah 61,54

8. Ny. Maya Mustikarini 58,24

9. Ny. Santoso 48,70

10. Ny. Nofik Mayasari 105,60

11. Ny. Avilia Ayu 114,25

12. Ny. Rianti 108,51

13. Ny. Hutri 124,68

14. Ny. Husnun Nuhlah 61,54

15. Ny. Rufita 48,70

16. Ny. Siti Ma’unah 49,03

17. Ny. Iswa Nur Mazidah 49,24

18. Ny. Eka Sriyanti 69,85

19. Ny. Isriyah 53,54

20. Ny. Nandifah 53,00

21. Ny. Erniyawati 53,65

22. Ny. Kukuh 56,21

23. Ny. Khoirun Nisa 53,11

24. Ny. Dwicha Novianti 61,54

25. Ny. Rofiah 74,43

26. Ny. Anik 68,57

27. Ny. Ani Nurcahyani 64,95

28. Ny. Avida istiqomah 74,43

29. Ny. Nia Putri Ayu 68,57

30. Ny. Khusnul Atika 70,70

31. Ny. Lina Setyara 61,54

32. Ny. Siti Nur Safitri 134,18

33. Ny. Rina Purnawati 142,71

34. Ny. Tantai 139,44

35. Ny. Khalifatul Aliyah 113,68

36. Ny. Musaqqif Ubaidah 80,96

37. Ny. Devi Achayani 107,05

38. Ny. Siti Fauzia 99,30

39. Ny. Eni Rohmawati 80,10

40. Ny. Lutfiyah 83,43

41. Ny. Nur Afifah 94,48

42. Ny. Dian Alawiyah 112,10

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100

NO. NAMA HASIL PIBF

(ng/ml)

43. Ny. Robiatul Adawiyah 79,88

44. Ny. Mudayanik 138,24

45. Ny. Reza Farida 96,99

46. Ny. Solikhatun 112,10

47. Ny. Siti Munawaroh 98,75

48. Ny. Anisa 114,25

49. Ny. Khairun Nisa 111,43

50. Ny. Wahyu Listiyaningsih 191,14

51. Ny. Nur Istiqomah 98,20

52. Ny. Ernawati 139,68

53. Ny. Ayu Dewi Lestari 83,43

54. Ny. Erni Purwanti 106,94

Semarang, 21 Juli 2022

Koordinator Sub Lab. GAKI FK UNDIP

Prof. Dr. dr. Banundari Rachmawati, SpPK(K)

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106 Lampiran 7. Dokumentasi penelitian

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