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Table S-1: Histology Scoring Method
Histologic
Feature Definition Score
0 1 2 3 4 5 6 7 8 9 10
Ca p sul e
Cellularity
# per 40x objective lens from 10 spotsin region of interest <30 31-60 61-90 91-120 121-150 151-180 181-210 211-240 241-270 271-300 >300
Fibroblast
# per 40x objective lens from 10 spotsin region of interest <30 31-60 61-90 91-120 121-150 151-180 181-210 211-240 241-270 271-300 >300
Adhesions
% of capsule adhered to surrounding humerus and radial (anterior) or ulnar (posterior) bone surfaces
<10 <15 <20 <25 <30 <40 <50 <60 <70 <80 >80
Fibrosis
% of capsule containing dense,unorganized connective tissue <10 <15 <20 <25 <30 <40 <50 <60 <70 <80 >80
Synovium
# of synovial cell layers from jointspace surface 1 2 3 4 5 6 7 8 9 10 >10
Thickness
measured in 3 locations (um), averaged and normalized to historical control data by subtracting 200 um
0-150 151-250 251-350 351-450 451-550 551-650 651-750 751-850 851-950 951-1050 >1050
***Taken from the Mankin scoring system and modified as follows
Ca rtil a g e
Structure
See detailed definitions →0 1a 1b 1c 2a 2b 3a 3b 4 5 6
Normal <30% 31-61% >60% <50% >50% <50% >50% Clefts to radial
zone
Clefts to calcified zone
Complete disorganization Surface irregularities
Pannus and surface irregularities
Clefts to transitional
zone
Cellularity
See detailed definitions →0 1a 1b 1c 1d 2a 2b 2c 3a 3b 3c
Normal <25% 25-50% 51-75% >75% <30% 31-61% >60% <25% 25-50% >50%
Diffuse hypercellularity Cloning Hypocellularity
Matrix staining
% of metachromatic staining that
resembles normal cartilage >80 <80 <70 <60 <50 <40 <30 <25 <20 <15 <10
Tidemark Integrity
% not crossed by blood vessels at the radial zone and calcified cartilage interface
>80 <80 <70 <60 <50 <40 <30 <25 <20 <15 <10
