Day 1 TLR5: monocyte subsets
No SIRS SIRS No SIRS SIRS 0
4000 8000 12000
MFI
Day 2 TLR5: monocyte subsets
No SIRS SIRS No SIRS SIRS 0
4000 8000 12000
MFI
Pre-operative TLR5: monocyte subsets
No SIRS SIRS No SIRS SIRS 0
4000 8000 12000
MFI
Day 2 TLR4: monocyte subsets
No SIRS SIRS No SIRS SIRS 0
5000 10000 15000 20000
MFI
Day 1 TLR4: monocyte subsets
No SIRS SIRS No SIRS SIRS 0
5000 10000 15000 20000
MFI
Classical Intermediate Classical Intermediate Classical Intermediate
Classical Intermediate Classical Intermediate Classical Intermediate
**
**
***
**
Pre-operative TLR4: monocyte subsets
No SIRS SIRS No SIRS SIRS 0
5000 10000 15000 20000
MFI
A B C
D E F
Supplemental Digital Content 7. Expression of TLR4 and TLR5 is higher on intermediate monocytes compared to classical monocytes at all @me points. Pre-‐opera9ve (A, D), day 1 (B, F) and day 2 (C, F) TLR4 (A-‐C) and TLR5 (D-‐F) expression on classical and intermediate monocytes.
