MALAYSIAN JOURNAL OF Medicine and Health Sciences
Vol. 16 No. SUPP14, Dec 2020 Supplementary Issue:
INTERNATIONAL PHYSIOLOGY SEMINAR &
ANNUAL MEETING OF INDONESIAN PHYSIOLOGY SOCIETY (SIPSAM 2019)
Vol. No. SUPP14 / Dec 2020 Supplementary Issue:
INTERNATIONAL PHYSIOLOGY SEMINAR & ANNUAL
MEETING OF INDONESIAN PHYSIOLOGY
SOCIETY (SIPSAM 2019) 16
Vol. 16 No. SUPP14 , December 2020
The Malaysian Journal of Medicine and Health Sciences (MJMHS) is published by the Faculty of Medicine and Health Sciences, Universiti Putra Malaysia. The main aim of the MJMHS is to be a premier journal on all aspects of medicine and health sciences in Malaysia and internationally.
The focus of the MJMHS will be on results of original scientific research and development, emerging issues and policy analyses pertaining to medical, biomedical and clinical sciences. The Malaysian Journal of Medicine and Health Sciences is now indexed in the following data bases:
Scopus, EBSCOhost, ISC, and Rubriq.
Editor in Chief:
Prof. Dr. Normala Ibrahim Members:
Prof. Dr. Rukman Awang Hamat Assoc. Prof. Dr. Rajesh Ramasamy Assoc. Prof. Dr. Sharmili Vidyadaran Assoc. Prof. Dr. Sethu Thakachy Subha Assoc. Prof. Dr. Loh Su Peng
Assoc. Prof. Dr. Nor Afiah Mohd Zulkefli Assoc. Prof. Dr. Wan Aliaa Wan Sulaiman Assoc. Prof. Dr. Ling King Hwa
Assoc. Prof. Dr. Habibah Abdul Hamid Assoc. Prof. Dr. Zubaidah Nor Hanipah Assoc. Prof. Dr. Ching Siew Mooi Assoc. Prof. Dr. Intan Hakimah Ismail
Lead Guest Editor:
Dr. Muflihatul Muniroh Guest Editors:
Dr. Hardian
Dr. Endang Mahati
Technical Coordinator:
Dr. Tan Kit Aun
Prof. Dr. Pierce Anthony Grace Prof. Dr. David Isaacs
Prof. Dr. P. Ronan O’Connell
Prof. Dr. Graham Alexander Mc Gregor Prof. Dr. Tan Ser Kiat
Prof. Dr. Gregory Y.H. Lip Prof. Dr. Roger Pepperell
About the Journal
Malaysian Journal of Medicine and Health Sciences Vol. 16 SUPP14, December 2020
Contents
ORIGINAL ARTICLES
High-protein Diet and Exercise Improve Inflammatory Marker and Oxidative Stress After Weight Loss Program in Adolescents Obesity: A Randomized Control Trial
Etika Ratna Noer, Luthfia Dewi, Darmawati Ayu Indraswari, Suroto, Martha Ardiaria
The Effect of Autonomous Sensory Meridian Response to Short-term Memory Function Among Young Adult Population in Indonesia Epifani Angelina Chandra, Tanjung Ayu Sumekar, Muflihatul Muniroh, Hardian Hardian
Ataxia and Cerebellar Dysfunction in Low Dose Methylmercury-induced Balb/c Mice
Alya Nisrina Fadhila, Nur Afifah Thohiroh, Yuriz Bakhtiar, Hardian Hardian, Vega Karlowee, Muflihatul Muniroh Sleep Insufficiency Influence on Nitric Oxide Concentration and Systolic Blood Pressure in Medical Students Ermin Rachmawati, Riskiyana
Direct Effects of Carbon Dioxide-rich Water Bathing on Peripheral Blood Flow
Andi Rizky Arbaim Hasyar, Noor Yasni Muchlis, Yahya Dwitama, Irfan Idris and Irawan Yusuf Correlation Between Ferritin and Thyroid Hormones in Obesity
Meita Hendrianingtyas, Satrio Adi Wicaksono, Lydia Purna Widyastuti Setjadiningrat Kuntjoro, Maharani, Sulistiyati Bayu Utami
Effect of Mangosteen (Garcinia Mangostana L.) Peel Supplementation on Alkaline Phosphatase Serum in Male Students After Heavy Exercise Rika Nailuvar Sinaga, Zulaini, Fajar Apollo Sinaga, Jumadin IP
The Effect of Citrus Peel Extract (Citrus Reticulata) on Hippocampal Histopathological Appearance in Wistar Rats Induced by Trimethyltin Chloride Fini Andriani, Talitha Salsabila, Bilqis Nabilah, Endang Kumaidah, Hardian, Ainun Rahmasari Gumay
The Association Between Plasma Natural Antibodies and Inflammatory Biomarkers Two Weeks After Calving in Cows with No Dry Period Novi Mayasari, Henk K. Parmentier, Erminio Trevisi, Bas Kemp, Ariette van Knegsel
Chemopreventive Effect of Carica Pubescens Leaf Extract on Neutrophil-lymphocyte Ratio, Erythrocyte Count, and Colon Histopathological Appearance of Dimethylhydrazine-induced Colon Cancer Rats
Ainun Rahmasari Gumay, Saekhol Bakri, Devi Oktavia, Kurnia Vanie Saritsya, Dwi Retnoningrum, Darmawati Ayu Indraswari, Indah Saraswati, Hermawan Istiadi, Yosef Purwoko, Muflihatul Muniroh, Hardian
Regular High Intensity Circuit Training Improves Attention Function and Reaction Time Among Male Young Adults
Tiara Augustina Putri, Muflihatul Muniroh, Yosef Purwoko, Ainun Rahmasari Gumay, Tanjung Ayu Sumekar, Endang Ambarwati
Correlation Between Serum Magnesium Level and Sarcopenia Occurence in the Elderly Women: Study with Dual-energy X-ray Absorptiometry (DXA) Audrianto Suranto, Sukmaningtyas Hermina, Ngestiningsih Dwi, Batubara Lusiana
The Effect of Additional Air Stacking Exercise on VO2 Max A Study on Elderly with Restrictive Pulmonary Disorder Who Had Deep Breathing Exercise Lisa Nurhasanah, Sri Wahyudati
The Effect of Plyometrics Training on Memory Function and Mood State Among Medical Students
Mohamad Dhiaulhaq, Adinda Marisna Putri, Mahardika Budjana Sutan Ilham, Ainun Rahmasari Gumay, Edwin Basyar, Darmawati Ayu Indraswari High Seafood Intake During Pregnancy and Low Blood Pressure Among Coastal Pregnant Women in Indonesia
Anindita Cahya Luthfita, Muflihatul Muniroh, Saekhol Bakri, Ainun Rahmasari Gumay, Hardian Hardian, Ariawan Ditya Birawa, Mulyono Mulyono, Nadia Afiyani, Lia Farhatania, Julian Dewantiningrum
Improvement of Sleep Quality by Autonomous Sensory Meridian Response (ASMR) Stimulation Among Medical Students
Hardian Hardian, Sari Satya Febriani, Tanjung Ayu Sumekar, Muflihatul Muniroh, Darmawati Ayu Indraswari, Yosef Purwoko, Endang Ambarwati Apium Graveolens Linn Affects Fibroblast and Collagen Density on the Incision Wound Healing
Tan Hengky, Indah Saraswati, Hermawan Istiadi, Vega Karlowee, Muflihatul Muniroh, Endang Mahati
Effect of Turmeric Powder and Extract on the Level of Triglyceride, Total Cholesterol and Liver Histopathological Appearance in Alloxan-induced Wistar Rats Vanessa Andhani Putri, Kiki Nilasari, Annisa Dentin, Akhmad Ismail, Hermawan Istiadi, Tanjung Ayu Sumekar, Muflihatul Muniroh, Ainun Rahmasari Gumay
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107 Laxative Effect of Kersen (Muntingia calabura) Leaf Extract with Multilevel Dose on Wistar Rats Induced Gambir
Nopiane Rospita Ingan Ergani, Lathifatul Hamidah, Bimanugraha, Dwi Retnoningrum, Dwi Marliyawati, Anna Mailasari Kusuma Dewi
The Relationship of Blood Glucose and LDL Level with Pulsatility Index of Cerebral Arteries Examined by Transcranial Color-coded Duplex Ultrasonography Dodik Tugasworo, Aditya Kurnianto, Yoshua Kevin Poonatajaya
CASE REPORT
Ten Years Neglected Chronic Subdural Hematoma of Pediatric in Rural Area Yuriz Bakhtiar, Lathifa Putry Fauzia, Adrian Pratama
Mal J Med Health Sci 16(SUPP14): 34-38, Dec 2020 34
Malaysian Journal of Medicine and Health Sciences (eISSN 2636-9346)
ORIGINAL ARTICLE
Effect of Mangosteen (Garcinia Mangostana L.) Peel Supplementation on Alkaline Phosphatase Serum in Male Students After Heavy Exercise
Rika Nailuvar Sinaga, Zulaini, Fajar Apollo Sinaga, Jumadin IP
Teaching Staff of Sports Science Faculty Universitas Negeri Medan (UNIMED), 20221 Medan, Indonesia
ABSTRACT
Introduction: Physical exercise can increase metabolism in the liver due to the presence of alkaline phosphatase (ALP), which subsequently causes the oxidative stress. In order to reduce this effect, the consumption of natural antioxidant is commonly performed, and one of these antioxidants could be found in mangosteen peel that contains high content of Xanthone and Procyanidin. This study aims to understand the effect of mangosteen peels to alkaline phosphatase serum in male student group who have performed heavy exercises. Methods: A true experimental study was carried out with pre- and post-test, and 21 male students were recruited which were divided into two groups. To understand the effect, a control group which was instructed to prescribe placebo was involved, whereas the other group was invited to consume mangosteen peels with dosage of 550 mg as their supplements. Both of the groups were prescribed for two weeks before performing the exercise, in which they were invited to perform treadmill exercise which had 75%-85 of intensity for three times a week. The ALP levels were measured on the final day of treatments after performing the heavy exercise. Results: A significant rise of ALP levels were demonstrated by the control group with p value <0.05, in contrast the group who has consumed the mangosteen peel as their supplements had significantly lower ALP levels compared to the placebo group. Conclusion: It is concluded that mangosteel peels had the ability in lowering the ALP serum levels in male students who have performed heavy exercise.
Keywords: Mangosteen, Alkaline phosphatase, Heavy exercise
Corresponding Author:
Rika Nailuvar Sinaga, M.Biomed Email: [email protected] Tel: +081375271485
INTRODUCTION
A physical exercise is defined as the physical activity performed by individual due to certain purposes for adjusting, managing, or improving the condition of body-parts, in which it is regularly performed with structured plans (1). As it is purposively planned and regularly performed, this exercise has been reported to prevent certain physical and mental diseases including hypertension, diabetes, osteoporosis and obesity;
depression respectively, as well as cancer (2).
The movements performed in physical exercise increase the oxygen consumption done by the body via the muscle fibers (3). However, at the same time, blood flow and metabolism decrease significantly resulting an increase of liver oxidative stress (4), in which this biological mechanism might have affected the enzyme activities
within the liver. Oxidative stress can be measured by assessing levels of malondialdehyde (MDA), this is because MDA is the result of lipid peroxidation caused by free radicals during physical exercise (5). Several studies have reported that exercise can increase Alanine aminotransferase (ALT), Alkaline phosphatase (ALP) and aspartate aminotransferase (AST) (6,7,8).
In lowering the risk of oxidative stress caused by unbalanced features in the liver system, natural and synthetic antioxidants have been suggested to be consumed (5,9). Natural antioxidants as liver protectors have been investigated; a purple sweet potato can reduce the level of hepatitis index enzymes in serum such as aspartate aminotransferase (AST) and alanine aminotransferase (ALT) (10). The effect of natural antioxidants on several enzyme parameters in the liver has also been studied by using the ethanol extracts of Ziziphus mauritiana leaves in reducing the levels of AST, ALT and ALP serum levels by involving rats as the subjects (11). Furthermore, Tulbaghia violacea rhizomes can reduce AST, ALT and ALP levels in atherosclerotic mice that suffer from liver damage (12).
Mal J Med Health Sci 16(SUPP14): 44-49, Dec 2020 44
Malaysian Journal of Medicine and Health Sciences (eISSN 2636-9346)
ORIGINAL ARTICLE
The Association Between Plasma Natural Antibodies and Inflammatory Biomarkers Two Weeks After Calving in Cows with No Dry Period
Novi Mayasari1, Henk K. Parmentier2, Erminio Trevisi3, Bas Kemp2, Ariette van Knegsel2
1 Laboratory of Physiology and Biochemistry, Faculty of Animal Science, Universitas Padjadjaran, Jl. Raya Bandung Sumedang KM 21, Jatinangor, 45363, Indonesia,
2 Adaptation Physiology Group, Department of Animal Sciences, Wageningen University, P.O. Box 338, 6700 AH Wageningen, the Netherlands
3 Department of Animal Sciences, Food and Nutrition (DIANA), Faculty of Agriculture, Food and Environmental Sciences, Università Cattolica del Sacro Cuore, 29122 Piacenza, Italy
ABSTRACT
Introduction: Improved energy balance, metabolic status, and natural antibodies (NAb) has been shown in cows with no dry period, however these cows showed increased inflammation status in early lactation. The aim of this study was to determine the association between plasma natural antibodies and inflammatory biomarkers in cows with no dry period during the first two weeks postpartum. Methods: Holstein-Friesian dairy cows (n=55) were selected. Before enroll to the experiment, cows were clinically healthy. Plasma samples were collected at week 1 and 2 after calving and were analyzed for NAb binding megantura-keyhole limpet hemocyanin and inflammatory biomarkers. Results: Cows with no dry period in this study had an improved energy balance and maintain NAb titers but increased ceruloplasmin (inflammatory biomarkers) in early lactation. In this study we found a significant correlation between NAb IgG binding KLH and haptoglobin in plasma (P<0.01). However, there were no correlations between albumin, cholesterol and NAb (IgG and IgM) binding KLH. Conclusion: This study demonstrate that cows with no dry period have an improved energy balance and maintained the level of natural antibodies in plasma. Moreover, IgG titers in plasma might be correlated with haptoglobin due to inflammation during calving until 2 wk postpartum.
Keywords: Continuous milking, Inflammation, Antibodies
Corresponding Author:
Novi Mayasari, PhD
Email: [email protected] Tel: +62-81214609646
INTRODUCTION
During transition period, immune status in dairy cows were suppressed and need to be increased. It is known that dairy cows are characterized with immune suppression during transition period, which is related with severe negative energy balance (EB), and high rate of infection diseases and metabolic disorders (8). Innate immunity is the first line defense against infection (1), and natural antibodies (NAb) are a part of humoral innate immunity before get any antigenic stimulation (2). CD5+ B-1 cells produce natural antibodies in healthy individuals and NAb mainly consist of immunoglobulin M (IgM), IgG and IgA (3,4). In previous research, NAb binding keyhole limpet hemocyanin (KLH) were higher in cows with an
improved EB in early lactation (7). Transition period is the crucial time for dairy cows especially in the first two weeks after calving. In early lactation, cows experienced negative EB, which is related to immunosuppression (9). Negative EB was not only related to NAb but also was associated with enhanced level of inflammatory biomarkers (10) and metabolic disorders (11) in dairy cows during early lactation.
In early lactation, increased disease rates are commonly reported among high-yielding dairy cows and characterized by the occurrence of an inflammatory response indicated by acute phase protein (APR) (12).
Inflammation evokes white blood cells (WBC) to release of tumor necrosis factor-alpha (TNF-α) and (interleukin-1 and -6) (IL 1 or 6). As a consequence, TNF-α and IL-1 or 6 triggered the release of acute phase response (13).
During the response of acute phase protein, positive acute phase reactants (+AP) including haptoglobin and ceruloplasmin were increased in plasma and negative
