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HASIL PENILAIAN SEJAWAT SEBIDANG ATAU PEER REVIEW KARYA ILMIAH : PROSIDING

Judul Karya Ilmiah : Improvement of Stability and Antioxidant Activities by Using Phycocyanin - Chitosan Encapsulation Technique

Jumlah Penulis : 5 Orang

Status Pengusul : Penulis ke-1

Identitas Prosiding : a. Judul Prosiding : IOP Conference Series: Earth and Environmental Science, 2nd International Conference on Tropical and Coastal Region Eco Development 2016

b. ISBN/ISSN : Online ISSN: 1755-1315, Print ISSN: 1755-1307 c. Thn Terbit, Tempat Pelaks. : 2017, Bali - Indonesia

d. Penerbit/Organiser : IOP Publishing Ltd

e. Alamat Repository/Web : https://iopscience.iop.org/article/10.1088/1755- 1315/55/1/012052

Alamat Artikel : https://iopscience.iop.org/article/10.1088/1755- 1315/55/1/012052/pdf

f. Terindeks di (jika ada) : Scopus Kategori Publikasi Makalah :

√ Prosiding

Forum Ilmiah Internasional (beri

pada kategori yang tepat) Prosiding

Forum Ilmiah Nasional Hasil Penilaian

Peer Review

:

Komponen Yang Dinilai

Nilai Reviewer

Nilai Rata- Reviewer I Reviewer II rata

a. Kelengkapan unsur isi prosiding (10%) 2,60 3,00 2,80

b. Ruang lingkup dan kedalaman pembahasan (30%) 8,00 7,00 7,50

c. Kecukupan dan kemutahiran data/informasi dan metodologi (30%)

8,00 7,00 7,50

d. Kelengkapan unsur dan kualitas terbitan/prosiding(30%) 8,50 9,00 8,75

Total = (100%)

27,10 26,00 26,55

Nilai Pengusul = (60% x 26,55) = 15,93

Semarang, Reviewer 2

Prof. Dr. Istadi, S.T., M.T.

NIP. 197103011997021001

Unit Kerja : Teknik Kimia FT UNDIP

Reviewer 1

Prof. Dr. Widayat, S.T., M.T.

NIP. 197206091998031001

Unit Kerja : Teknik Kimia FT UNDIP

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Document details

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Improvement of Stability and Antioxidant Activities by Using Phycocyanin - Chitosan Encapsulation Technique

(Conference Paper) (Open Access)

, , , ,

Chemistry Department, Diponegoro University, Semarang, Indonesia

Chemcal Engineering Department, Diponegoro University, Semarang, Indonesia Physic Department, Diponegoro University, Semarang, Indonesia

Abstract

Encapsulation is a coating process to improve the stability of bioactive compounds. Phycocyanin with high antioxidant activity has been encapsulated with chitosan in microcapsules form. In this study aims to determine the best conditions in the encapsulation process using the extrusion method, characterization of the physicochemical properties of the microcapsules, antioxidant activity test using DPPH, in vitro release performance and evaluate the storage stability against temperature. The results of the encapsulation process is obtained: Na-TPP is better than Na- citrate as crosslinker and chitosan content 3% as a coating with ratio of chitosan to phycocyanin ratio 1: 1. Test of antioxidant activity also showed encapsulation with chitosan content 3% has the highest antioxidant activity.

Morphological analysis microcapsules were found to have compact spherical shape with diameter range 900-1000 μm.

In vitro release testing showed a quick release in an acidic environment (SGF) for 2 hours and slowly release under alkaline conditions (SIF) for 8 hours under mechanical stirring at 37°C. Phycocyanin much more stable against temperature during storage in microcapsules. © Published under licence by IOP Publishing Ltd.

SciVal Topic Prominence

Topic:

Prominence percentile: 97.637

Author keywords

chitosan Encapsulation phycocyanin stability

Indexed keywords

Engineering controlled terms:

Antioxidants Chitin Chitosan Coastal zones Coatings

Convergence of numerical methods Microstructure Sodium Stability

Engineering uncontrolled terms

Alkaline conditions Anti-oxidant activities Bioactive compounds Encapsulation process Mechanical stirring Morphological analysis Phycocyanin Physicochemical property

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IOP Conference Series: Earth and Environmental Science Volume 55, Issue 1, 1 March 2017, Article number 012052

2nd International Conference on Tropical and Coastal Region Eco Development 2016, ICTCRED 2016; Bali; Indonesia; 25 October 2016 through 27 October 2016; Code 126824

Suzery, M.â  Hadiyanto^b  Majid, D.â Setyawan, D.â Sutanto, H.^c 

a b c

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Spirulina | Phycocyanin | Light intensity

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, , (2020) Food and Bioproducts Processing

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Encapsulation of phycocyanin by electrospraying: A promising approach for the protection of sensitive compounds Schmatz, D.A. da Silveira Mastrantonio, D.J. Vieira Costa, J.A.

The 21st century revival of chitosan in service to bio-organic chemistry

Yaneva, Z. Ivanova, D.

Nikolova, N.

Utilization of carrageenan and chitosan as coating material in phycocyanin encapsulation Hadiyanto Sutanto, H. Suzery, M.

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Source Type: Conference Proceeding Original language: English

DOI: 10.1088/1755-1315/55/1/012052 Document Type: Conference Paper Volume Editors: Hadiyanto Sponsors:

Publisher: Institute of Physics Publishing

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meinysuzery10@gmail.com

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4/7/2020 The 2nd International Conference on Tropical and Coastal Region Eco Development (ICTCRED 2016) - LPPM Universitas Diponegoro

lppm.undip.ac.id/v1/2016/06/16/call-for-paper-2nd-international-conference-on-tropical-and-coastal-region-eco-development-ictcred-2016-bali-indonesi… 1/2

mLain-lain Conference, ICTCRED

The 2nd International Conference on Tropical and Coastal Region Eco Development (ICTCRED 2016) will be held in Inna Grand Bali Beach Hotel, Sanur, Bali, Indonesia at October 25-27, 2016. The conference provides an excellent opportunity to share and exchange technologies and applications in the area of Tropical and Coastal Region Eco- Development. The previous event (the 1st ICTCRED 2014) has been successfully organized in Semarang, Indonesia.

We cordially invite prospective scholars, academicians and scientists to submit research paper to this conference.

SCOPE

The conference is intended to promote and disseminate all research papers under scope of : (A). Coastal Region Eco- Development, and (B). Tropical Life Sciences. The scope of Coastal Region Eco Development encompasses publications with topics related to (but not limited to): Coastal engineering, Marine Products Processing, Marine Biotechnology, Climate Change, Coastal Management and Social Economics, Disaster management, Food Technology and any other relevant science and engineering subjects related to coastal eco-developments.

While the scope of Tropical Life Sciences includes: molecular biology, public health, psychology, tropical diseases, policy related to health and disease; pharmacological aspects and treatment, epidemiology, genetic studies, medical microbiology, food nutrition and health, and all related fields of tropical life sciences.

PUBLICATION

We proudly announce that we have reached an agreement with IOP (Institute of Physics) to publish the 2nd ICTCRED 2016 conference proceeding into IOP Conference Series: Earth and Environmental Science (EES) (indexed by SCOPUS – Online ISSN: 1755-1315 Print ISSN: 1755-1307). http://conferenceseries.iop.org/content/forthcoming (click on EES tab)

KEYNOTE SPEAKERS

1. Prof. Ocky Karna Rajasa (Director of Research and Public Services at Ministry of Research, Technology and Higher Education – Indonesia) : “Policy on Coastal Research in Indonesia”

2. Prof. Ambariyanto (Diponegoro University – Indonesia) : “Updates in Coastal Development”

3. Prof. Chin Kun Wan (Chun Shan Medical University – Taiwan / President of International Society for Nutraceutical and Functional Foods) : “Current Progress in Nutraceutical for Tropical Disease”

4. Prof. Makoto Tsuchiya (University of the Ryukyus – Japan) :“Coral Reef Ecosystem”

5. Prof. Cesarettin Alasarvar(TÜBİTAK Marmara Research Center, Food Institute – Turkey) :“Marine Functional Foods”

lLPPM 16/06/2016

A

LPPM Universitas Diponegoro

>

Lain-lain

>

The 2nd International Conference on Tropical and Coastal Region Eco Development (ICTCRED 2016)

The 2nd International Conference on Tropical and

Coastal Region Eco Development (ICTCRED 2016)

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4/7/2020 The 2nd International Conference on Tropical and Coastal Region Eco Development (ICTCRED 2016) - LPPM Universitas Diponegoro

lppm.undip.ac.id/v1/2016/06/16/call-for-paper-2nd-international-conference-on-tropical-and-coastal-region-eco-development-ictcred-2016-bali-indonesi… 2/2

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6. Prof. Peter Gell (Federation University of Australia – Australia) : “Pollution and Coastal Environmental Changes in Tropical Region”

7. Prof. Winterwerp Han (TU Delft – Netherland) :“Hybrid Engineering for coastal abrasion prevention”

IMPORTANT DATES

Abstract submission deadline : May 31th, 2016 Acceptance notification : July 1st, 2016

Early bird registration : August 21th, 2016 Full Text Submission : October 1th, 2016

ICTCRED 2016 Conference : October 25th – 27th 2016 AUTHOR GUIDELINES & SUBMISSION

Author should first register to the online manuscript submission system on http://ictcred.org After registration, Author can submit the abstract.

Abstract template (in MS Word) : https://goo.gl/NrMNiV

The full paper should be prepared following the template from Institute Of Physics (IOP).

BEST PAPER & PRESENTER AWARDS

The committee will grant awards based on two categories: best paper and best presenter. The nominees selection for each category will be determined by the Scientific Committee.

MISCELLANEOUS

The information that appears in this announcement is correct as of May 1, 2016.

For further information please visit: http://ictcred.org

Additional queries can be addressed to: ictcred@live.undip.ac.id

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Published in IOP Proceeding Series: 2

^nd

International Conference on Tropical and Coastal Region Eco Development (ICTCRED) 2016

PREFACE

The 2

^nd

International Conference on Tropical and Coastal Region Eco Development (ICTCRED) 2016 was held by Research and Community Services Institute, Diponegoro University, Indonesia in Bali during 25- 27 October 2016. Bali, the god island amid pleasant surroundings, was a delightful place for this conference.

The 165 research papers were presented both by oral and poster presentation, and the conference had delivered many fruitful discussions and exchanges that contributed to the success of the conference. Among these papers, we selected 72 papers through intensive review by scientific committee. Participants from 10 countries made the conference truly international in scope and the presentations were split into two main conference areas, i.e., tropical life sciences and Coastal Region Developments. There were 7 plenary lectures covering the different areas of the conference: Prof. Ocky Karna Radjasa (Ministry of Research, Technology and Higher Education) talked on Policy on Coastal Research in Indonesia, Prof. Ambariyanto (Diponegoro University) on Update in Coastal Development, Prof. Makoto Tsuchiya (University of the Ryukyus – Japan) on Coral Reef Ecosystem, Prof. Peter Gell (Federation University of Australia – Australia) on Pollution and Coastal Environmental Changes in Tropical Region, Prof. Johan C. Winterwerp (TU Delft, Netherlands) on Restoring eroding mangrove-mud coasts through Building with Nature. These plenary speakers have given significant scientific contributions to the conference.

Participants have delivered their talks of valuable research outputs which are varies from extracting valuable compounds from marine environment and convert them to specialty products. During the presentation, marine products and their processing were also elaborated with developments in oceanography technology, coastal environment management and policies. These topics were more fruitful when researches in social science and its developments especially at the coastal area were also presented.

These wide range of topics have colorized this conference.

Finally, it is appropriate that we record our thanks to our fellow members of the Organizing Committee and of the Scientific Committee for their work in maintaining high quality of papers and in encouraging participation from those areas. We are also indebted to those who served as chairman of Institute of Research and Community Services, Diponegoro University and Ministry of Research, Technology and Higher Education for their financial supports. Without their support, the conference could not have been the success that it was. We also acknowledge the authors themselves, without whose expert input there would have been no conference. The continuing success of this conference series means that planning can now proceed with confidence for the same event in 2017.

Guest Editors

Hadiyanto,

Chair of Scientific Committee

1

2nd International Conference on Tropical and Coastal Region Eco Development 2016 IOP Publishing IOP Conf. Series: Earth and Environmental Science 55

(2017) 011001 doi:10.1088/1755-1315/55/1/011001

International Conference on Recent Trends in Physics 2016 (ICRTP2016) IOP Publishing Journal of Physics: Conference Series 755 (2016) 011001 doi:10.1088/1742-6596/755/1/011001

Content from this work may be used under the terms of theCreative Commons Attribution 3.0 licence. Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI.

Published under licence by IOP Publishing Ltd

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Scientific Committee/Editor

Hadiyanto (Diponegoro University, Indonesia) Makoto Tsuchiya (University of the Ryukyus, Japan) Heru Susanto (Diponegoro University, Indonesia)

Muhammad Mukhlisin (Semarang State Polytechnic, Indonesia) Jamari (Diponegoro University, Indonesia)

Hussein Gasem (Diponegoro University, Indonesia) Ambariyanto (Diponegoro University, Indonesia) Muhammad Zainuri (Diponegoro University, Indonesia) Muhammad Ali (Mataram University)

Sultana MH Faraz (Diponegoro University, Indonesia) Gerard Pals (Vrije Universiteit Amsterdam, Netherland)

Randi Hagerman (University of California Davis, United States) Flora Tassone (University of California Davis, United States) Herawati Sudoyo (Eijkman Institute, Indonesia) Ocky Karna Rajasa (MRTHE, Indonesia)

Craig Starger (Colorado State University, United States)

CN Ravishankar (Central Institute of Fisheries Technology, India) Agus Sabdono (Diponegoro University, Indonesia)

Maria Barbosa (Wageningen University, Netherland) Yasuhiro Igarashi (Japan)

Irwandi Jaswir (IIUM, Malaysia)

Michio Hidaka (University of the Ryukyus, Japan)

Organizing Committee

Munawar A Riyadi Chair

Tri Indah Winarni Co-Chair of TLS Diah Permata Wijayanti Co-Chair of CRED

Eko Susanto Secretary

Desrina Proceeding

Muh. Arfan Publication

This conference has been conducted by Institute of Research and Community Services, Diponegoro University, Indonesia 25-27 October 2016

2

2nd International Conference on Tropical and Coastal Region Eco Development 2016 IOP Publishing

IOP Conf. Series: Earth and Environmental Science 55

(2017) 011001 doi:10.1088/1755-1315/55/1/011001

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4/7/2020 IOP Conference Series: Earth and Environmental Science, Volume 55, 2017 - IOPscience

https://iopscience.iop.org/issue/1755-1315/55/1 1/12

 _NOTICE : Ensuring subscriber access to content on IOPscience throughout the coronavirus outbreak - see our remote access guidelines.

This study aims to determine the optimum conditions of the encapsulation process using the extrusion method thorugh characterization of the physicochemical properties of the microcapsules, antioxidant activity test using DPPH, in vitro release performance and evaluate the storage stability against temperature. The results showed that Na-TPP provided better encapsulation performance than Na-citrate as crosslinker at 3% chitosan content. The study of antioxidant activity also showed that at 3% chitosan concentration resulted highest antioxidant activity. The morphological analysis of microcapsules showed that the beads have compact spherical shape with diameter range of 900-1000 µm. In vitro release study demonstrated a quick release in an acidic environment (SGF) during 2 hours experiments and slow release under alkaline conditions (SIF) for 8 hours experiments under constant temperature at 37

^o

C. The encapsulation also showed that phycocyanin was more stable against temperature changes during storage.

Keywords: Encapsulation, stability, chitosan, phycocyanin

1. Introduction

Antioxidants are compounds that can counteract free radicals and an electron donating compound or a reductant. Antioxidants also are compounds that can inhibit the oxidation reaction, to scavenge free radicals and highly reactive molecules, which can inhibit cell damage [1]. Phycocyanin is a group of pigments that are bound to protein (biliprotein). Besides the potential as a natural dye, phycocyanin also known to have healing abilities, such as antioxidants [2] and anticancer [3]. But the application of phycocyanin very limited because it is vulnerable to light and temperature [4]. Phycocyanin can be damaged at temperatures above 30

^o

C [5], and a solution of phycocyanin undergo color fading by 30%

after 5 days of storage and become clear after 15 days at a temperature of 35°C [6]. Therefore, the need for technique to improve the phycocyanin stability but still maintain its antioxidant activity is required.

Encapsulation is a process of coating a core material by using a specific encapsulation materials [7].

Encapsulation can be used to protect the drug from environmental influences (humidity, light, heat), as well as to control the release of antioxidants to the targeted medications [8]. In the process of encapsulation, it needs proper matrix with the core material to be encapsulated.

1

2nd International Conference on Tropical and Coastal Region Eco Development 2016 IOP Publishing IOP Conf. Series: Earth and Environmental Science 55

(2017) 012052 doi:10.1088/1755-1315/55/1/012052

International Conference on Recent Trends in Physics 2016 (ICRTP2016) IOP Publishing Journal of Physics: Conference Series 755 (2016) 011001 doi:10.1088/1742-6596/755/1/011001

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Past and Future Ecosystem Change in the Coastal Zone

P Gell¹

1

Water Research Network, Federation University Australia, Ballarat, Australia E-mail: p.gell@federation.edu.au

Abstract. The coastal zone is in a constant state of flux. Long term records of change attest to

high amplitude sea level changes. Relative stability though the Late Holocene has allowed for the evolution of barrier dune systems, estuaries and coastal lakes with associated plant and faunal associations. This evolution has been interspersed with changes in the balance between climate driven changes in outflow from catchments. These interactions have been considerably disturbed through the impacts of industrialised people who have diverted and consumed water and invested in infrastructure that has impacted on river flows and the tidal prism in estuaries. This has impacted their provisioning services to humans. It has also impacted their regulating services in that development along the coastline has impacted on the resilience of the littoral zone to absorb natural climate extremes. Looking from the past we can see the pathway to the future and more easily recognise the steps needed to avoid further coastal degradation. This will increasingly need to accommodate the impacts of future climate trends, increased climate extremes and rising seas. Coastal societies would do well to identify their long term pathway to adaptation to the challenges that lie ahead and plan to invest accordingly.

Keywords. estuaries, paleolimnology, climate change, hydroecology, sediments, nutrients,

salinization

1. Introduction

The coastal zone is in a constant state of change. This is most evident through the daily cycles of the tides that, particularly in the macrotidal zones of the world, inundate and then strand the marine littoral zones. This alone demands that the biological communities affected by the rise and fall of tides be adaptable to inundation and exposure, and in estuaries, exposed variously by marine waters and those flowing from the hinterland which are usually fresh to oligosaline. This balance varies seasonally with, in temperate zones, wet seasons coinciding with destructive waves regimes leading to the opening of estuary mouths, while the dry season and associated constructive wave regimes lead to mouth closure and the establishment of lagoonal conditions. The consequences are a winter of tidal and river flushing with oxygenated water and a summer with little flow, stratification, hypersalinity and de-oxygenation.

These seasonal patterns are taken to extreme under multi-year (e.g. El Nino Southern Oscillation) and even multi-decadal (Inter-decadal Pacific Oscillation) phases of wet and dry conditions exacerbating or subduing the contrasts between seasons. Further the frequency of these cycles extends out to multi-

1

2nd International Conference on Tropical and Coastal Region Eco Development 2016 IOP Publishing IOP Conf. Series: Earth and Environmental Science 55

(2017) 012001 doi:10.1088/1755-1315/55/1/012001

International Conference on Recent Trends in Physics 2016 (ICRTP2016) IOP Publishing Journal of Physics: Conference Series 755 (2016) 011001 doi:10.1088/1742-6596/755/1/011001

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4/7/2020 Conserving endangered marine organisms: causes, trends and challenges - IOPscience

https://iopscience.iop.org/article/10.1088/1755-1315/55/1/012002 1/10

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PAPER • OPEN ACCESS

Conserving endangered marine organisms: causes, trends and challenges

Ambariyanto

Published under licence by IOP Publishing Ltd

IOP Conference Series: Earth and Environmental Science, Volume 55, 2nd International Conference on Tropical and Coastal Region Eco Development 2016 25–27 October 2016, Bali, Indonesia

Abstract

Increasing demand for marine resources in recent decades for human needs has led to intensified exploitation. This increase accelerates the process of extinction of various marine resources. In order to avoid extinction, it requires conservation measures of marine

resources appropriately. This paper provides an overview of causes of extinction, trends and

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1,2

ambariyanto@undip.ac.id ambariyanto.undip@gmail.com

Department of Marine Sciences, Faculty of Fisheries and Marine Sciences, Diponegoro University, Jl. Prof. H. Soedarto, SH, Tembalang Semarang. 50275

Coastal Disaster Rehabilitation and Mitigation Center, Integrated Laboratory, Diponegoro University, Jl. Prof. H. Soedarto, SH, Tembalang, Semarang. 50275

Ambariyanto 2017 IOP Conf. Ser.: Earth Environ. Sci. 55 012002 https://doi.org/10.1088/1755-1315/55/1/012002

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Determination and Radiocarbon Dating of Marine Mollusc Fossils in Ancient Sea Shelf of Central Java Indonesia

S Aisyah

¹

, D Pringgenies

²

, A Hartoko

³

, J T S Sumantyo

⁴

, H Matsuzaki

⁵

1

Master of Coastal Resource Management, Diponegoro University, Semarang 50275, Indonesia

2

Department of Marine Science, Faculty of Fisheries and Marine Science, Diponegoro University, Semarang 50275, Indonesia

3

Marine Geomatic Center and Department of Fisheries, Faculty of Fisheries and Marine Science, Diponegoro University, Semarang 50275, Indonesia

4

Josaphat Microwave Remote Sensing Laboratory, Center for Environmental Remote Sensing, Chiba University, 1-33 Yayoi, Inage, Chiba 263-8522, Japan

5

Micro Analysis Laboratory, Tandem accelerator (MALT), The University Museum, The University of Tokyo, Japan

Corresponding Author: sitiaisyah859@yahoo.com

Abstract. Mollusc phylum is one of the most adaptive animal groups on Earth. They occupy and thrive in incredibly diverse habitats. Their distribution in the fossil record is equally diverse.

Indonesia is one of the country with marine mollusc fossil variety, such as in the archaeological site of Sangiran, Patiayam (Ancient Muria Strait) and Grobogan – Central Java. Radiocarbon Dating is the method for dating analysis using

¹⁴

C. By measuring

¹⁴

C content, we can estimate how long ago the fossils died. Radiocarbon dating is an extremely useful technique for determining the ages of geological materials (that have some organic-derived carbon in them).

Field sampling had found variety of marine mollusc fossils such us 1) Sangiran: Dosinia sp., Telescopium telescopium, Anadara sp., and Dosinia insularum; 2) Patiayam (Ancient Muria Strait): Pseudodon vondenbuschianus, Elongaria orientalis, Conus (Pionoconus) sp. , Ampullina bandongensis, Anadara pilula and Filopaludina javanica; 3) Grobogan: Antigona chemnitzii, Cultelus dilatatus, Plotia scabra and Tibia modesta. Mollusc fossils sample was analysed using Accelerator Mass Spectrometer (AMS) radiocarbon system. It is a good method for dating specific samples. The result showed that Sangiran as an ancient deepsea has fossils age 38710 – 31947 years, then Patiayam (Ancient Muria Strait) with fossils age 26248 – 11994. Mollusc fossils from Grobogan was the youngest area has fossils age 25692 – 6479 years.

Keywords: Radiocarbon Dating, Mollusc Fossils, Patiayam, Grobogan, Sangiran 1. Introduction

The interrelationships and evolutionary history of molluscs have seen great advances in the last decade [1]. Indonesia is one of the country with marine mollusc fossil variety, such as in the Sangiran, Patiayam (Ancient Muria Strait) and Grobogan – Central Java. Long time periode, Indonesian archipelago had been formed through tectonic movement, volcanic eruption process, earthquake, sedimentation and erosion process simultaneously. Similar phenomenon had happened in ancient Muria Mountain with deformation phases of ocean go through with land had result sedimentation process in Demak, Jepara and Pati (the part of Central Java) to become ancient Muria Strait. Long time before Muria region had

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2nd International Conference on Tropical and Coastal Region Eco Development 2016 IOP Publishing IOP Conf. Series: Earth and Environmental Science 55

(2017) 012064 doi:10.1088/1755-1315/55/1/012064

International Conference on Recent Trends in Physics 2016 (ICRTP2016) IOP Publishing Journal of Physics: Conference Series 755 (2016) 011001 doi:10.1088/1742-6596/755/1/011001

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Antioxidant activity of three microalgae Dunaliella salina, Tetraselmis chuii and Isochrysis galbana clone Tahiti

Ita Widowati

¹

, Muhammad Zainuri

¹

, Hermien Pancasakti Kusumaningrum

²

, Ragil Susilowati

³

, Yann Hardivillier

⁴

, Vincent Leignel

⁴

, Nathalie Bourgougnon

⁵

, Jean-Luc Mouget

⁴

1

Department of Marine Science, Faculty of Fisheries and Marine Science, Diponegoro University, Jl. Prof. Soedarto SH, Tembalang, Semarang -50275 , Indonesia

2

Department of Biology, Faculty of Sciences and Mathematic, Diponegoro University, Jl. Prof Soedarto SH, Tembalang, Semarang -50275, Indonesia

3

Alumny Master of Coastal Ressource Management, Faculty of Fisheries and Marine Science, Diponegoro University, Jl. Prof.Soedarto SH Tembalang, Semarang-50275 , Indonesia

4

Laboratoire du Mer Molécule et Santé, Université du Maine, Le Mans, France

5

Laboratoire de Chimie Biologie Marine, Université de Bretagne Sud, Vannes, France

Email : ita_jusup@yahoo.co.id

Abstract. Natural alternatives antioxidant source has become a trending topic in the past decades to replace synthetic antioxidant. Microalgae have been mentioned to show interesting bioactive properties and one of them is its antioxidant activity. This study aims to evaluate the potential of three microalgae Dunaliella salina, Tetraselmis chuii and Isochrysis galbanaas new source of natural antioxidant. Proximate analysis and total phenolic content of D. salina, T. chuii and I.

galbanas were determined. Antioxidant activity of methanolic extracts of these three species prepared in different concentration (50, 100, 250, 500, and 1000 ppm) was performed through DPPH assay. I. galbana clone Tahiti demonstrated a highest antioxidant potential with 61.64 of inhibition at 50 ppm followed by D. salina with 58.45 % of inhibition and T. chuii with 52.58 % of inhibition. I. galbana clone Tahiti was the best antioxidant with total phenol content of 17.798 mg GAE g

^-1

extract at 50 ppm; followed by T. chuii 16.868 mg GAE g

^-1

extract and the lowest was D. salina with 4.672 mg GAE g

^-1

extract. Results suggest that these microalgae posses antioxidant potential which could be considered for future applications in medicine, dietary supplements, cosmetics or food industries.

Keywords: antioxidant activity, DPPH, microalgae, total phenol.

1. Introduction

Indonesia, as an archipelagic state surrounded by ocean, presents a potential as a microalgae source because of its huge natural biodiversity. Microalgae are distributed everywhere: in salt, brackish or freshwater, in tropical to cold regions and sometimes as symbionts with other organisms, and some species are used and cultivated by farmers.

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2nd International Conference on Tropical and Coastal Region Eco Development 2016 IOP Publishing IOP Conf. Series: Earth and Environmental Science 55

(2017) 012067 doi:10.1088/1755-1315/55/1/012067

International Conference on Recent Trends in Physics 2016 (ICRTP2016) IOP Publishing Journal of Physics: Conference Series 755 (2016) 011001 doi:10.1088/1742-6596/755/1/011001

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PDF LEMBAR HASIL PENILAIAN SEJAWAT SEBIDANG ATAU - Universitas Diponegoro

Judul Karya Ilmiah : Improvement of Stability and Antioxidant Activities by Using Phycocyanin - Chitosan Encapsulation Technique

Jumlah Penulis : 5 Orang

Status Pengusul : Penulis ke-1

Identitas Prosiding : a. Judul Prosiding : IOP Conference Series: Earth and Environmental Science, 2nd International Conference on Tropical and Coastal Region Eco Development 2016

b. ISBN/ISSN : Online ISSN: 1755-1315, Print ISSN: 1755-1307 c. Thn Terbit, Tempat Pelaks. : 2017, Bali - Indonesia

d. Penerbit/Organiser : IOP Publishing Ltd

e. Alamat Repository/Web : https://iopscience.iop.org/article/10.1088/1755- 1315/55/1/012052

Alamat Artikel : https://iopscience.iop.org/article/10.1088/1755- 1315/55/1/012052/pdf

f. Terindeks di (jika ada) : Scopus Kategori Publikasi Makalah :

Forum Ilmiah Internasional (beri

Forum Ilmiah Nasional Hasil Penilaian

:

a. Kelengkapan unsur isi prosiding (10%) 2,60 3,00 2,80

b. Ruang lingkup dan kedalaman pembahasan (30%) 8,00 7,00 7,50

c. Kecukupan dan kemutahiran data/informasi dan metodologi (30%)

8,00 7,00 7,50

d. Kelengkapan unsur dan kualitas terbitan/prosiding(30%) 8,50 9,00 8,75

Total = (100%)

Semarang, Reviewer 2

Prof. Dr. Istadi, S.T., M.T.

NIP. 197103011997021001

Unit Kerja : Teknik Kimia FT UNDIP

Reviewer 1

Prof. Dr. Widayat, S.T., M.T.

NIP. 197206091998031001

Unit Kerja : Teknik Kimia FT UNDIP

Document details

Improvement of Stability and Antioxidant Activities by Using Phycocyanin - Chitosan Encapsulation Technique

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C-Phycocyanin: A biliprotein with antioxidant, anti-inﬂammatory and neuroprotective eﬀects

C-Phycocyanin from Oscillatoria tenuis exhibited an antioxidant and in vitro antiproliferative activity through induction of apoptosis and G /G cell cycle arrest

Stability of phycocyanin extracted from Spirulina sp.: Inﬂuence of temperature, pH and preservatives

Phycocyanin from Spirulina sp: Inﬂuence of processing of biomass on phycocyanin yield, analysis of eﬃcacy of extraction methods and stability studies on phycocyanin

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