Penulisan Jurnal Ilmiah

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Prof. Ir. I Gede Putu Wirawan, M.Sc., Ph.D

UPT Laboratorium Sumberdaya Genetika dan Biologi Molekuler

Universitas Udayana

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Penelitian

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• Pendidikan dan Pengajaran di PT berbasis keahlian yang diperoleh dari PENELITIAN

• Salah satu bentuk hasil penelitian adalah

JURNAL (artikel pada jurnal ilmiah)

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Publikasi

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• Bukan Publikasi pada :

– Seminar/ Conference (oral atau poster)

– Media koran atau majalah umum

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Kenapa Jurnal ?

• Jurnal sebagai media komunikasi ilmiah

• Jurnal sebagai salah satu bentuk keberhasilan penelitian

• Jurnal sebagai media menguji kebaruan hasil penelitian

• Publikasi sebagai tolok ukur kinerja ILMUWAN di PT

• Publikasi sebagai tolok ukur kinerja dan penentu

peringkat PT

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Jurnal Ilmiah

• Dikelola dan Diterbitkan oleh Asosiasi Profesi

• Karena Profesi cendrung bersifat KHUSUS pada minat tertentu

• Jurnal mempunyai peringkat atau INDEX

• Pandai-pandai memilih

jurnal

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Bagamana memilih jurnal ?

• Ada hasil penelitian yang dipandang baik : original, ada novelty /kebaruan,

• Ada manuskrip yang dibuat

• Search jurnal di internat atau lewat teman yang sudah

biasa di jurnal yang dituju atau yang sudah banyak punya publikasi pada jurnal

• Ada yang mengkomunikasikan ke jurnal yang dituju (ada komunikator – biasanya adalah seorang Profesor atau

ilmuwan yang sudah dikenal baik karena sudah banyak

publikasinya pada jurnal tersebut))

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Memulai menulis jurnal

• Petakan data yang dimiliki

• Buat skenario manuskrip nya

• Buat draft abstract

• Buat draft pendahuluannya

• Tentukan judulnya

• Tentukan Jurnal yang dituju

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Menulis Jurnal Ilmiah

• Format : sesuaikan dengan yang diminta oleh jurnal yang dituju

• Secara Umum :

– Title dan Penulis serta afiliasinya – Abstract

– Introduction

– Materials and Methods – Results and Discussion – Acknowledgement

– Reference

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Judul/ Title and Authors

• Judul harus jelas, lugas, dan ringkas / singkat

• Judul mesti menggambarkan isi – kesimpulan

• Author and affiliation - Corresponding author

• Alamat/ e-mail corresponding author

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UTILIZATION OF ELECTRIC PULSED POWER ON

FRUITING OF EDIBLE MUSHROOMS

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Abstract

• Menggambarkan keseluruhan isi artikel yang dituliskan secara ringkas

• Dibuat dalam satu alinea saja

• Key world

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UTILIZATION OF ELECTRIC PULSED POWER ON FRUITING OF EDIBLE MUSHROOMS

Shoji Ohga

Division of Forest Environmental Sciences, Department of Agro- environmental Sciences,

Kyushu University, Fukuoka 811-2415, Japan [email protected]

ABSTRACT

Effect of pulsed power was investigated on fruit body formation of 10 edible mushrooms, Lentinula edodes, Glifola frondosa, Pholiota nameko, Flammulina velutipes, Hypsizygus marmoreus, Pleurotus ostreatus, Pleurotus eryngii, Pleurotus abalones, Agrocybe cylindracea and Sparassis crispa. Pulsed power of 100-170 kV was directly charged to the substrate just before fructification. The effect of the pulsed power resulted to promote for 10 edible mushrooms fructification. The treatment especially stimulated the fructification on Pleurotus species.

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ABSTRACT

Gene targeting events were analyzed in buckwheat (Fagopyrumesculentum, var. Shinano No. 1) that were inplantatransformed (T₁) by the three strains of Agrobacterium tumefaciensharboring a gene targeting vectorfor an endogenous gene (acc.no.AB327276); M-1 mutant (abvA::Tn5) and M-31 mutant (virA::Tn5) strains, both of which are capable of transferring its T- DNA into nucleus of host plant cell, but have a deficiency in T-DNA integration step into chromosome and LBA4404 strain, being a commonly used strain. The results of both phenotype examination and Southern blot analysis implied that a targeting construct(s) was integrated into a diﬀerent locus (loci) each depending on the strain of A. tumefaciens used for transformation. Both 5’-end and 3’-end flanking DNA segments that were expected from precise insertion of targeting construct into the endogenous U-gene locus were obtained by PCR with 3 (23%) transformants out of the randomly selected 13 transformants by M-1 mutant strain, while only 3’-end flanking DNA segment was obtained with 3(21%) transformants out of 14 transformants by M-31 strain. Taken together, the results suggested the potential of usage of a mutant strain of A.

tumefaciens for gene targeting which has a deficiency in T-DNA integration step into plant genome.

Keywords: Agrobacterium tumefaciens; gene targeting;

buckwheat;Fagopyrumesculentum; mutant strains ofA. tumefaciens

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Effect of the soil protection sheet and the bag material on the soil microorganisms and the indigenous arbuscular mycorrhizal fungi in the volcanic devastated site in Mt.

Batur, Bali, Indonesia

Nobuyuki Kohno*^1,4, Kayo Gouya*¹, Shin-ichi Sekiyama*¹, Motoyuki Suzuki*²and Wirawan, I Gede Putu^*3

*1 Takino Filter Inc., 904-16, Hayama 2-chome, Kudamatsu, Yamaguchi 744-0061 Japan

*2 Yamaguchi University, Tokiwadai 2-16-1, Ube, Yamaguchi 755-8611 Japan

*3 Faculty of Agriculture, Udayana University, Bali, Indonesia

*4 Corresponding author: [email protected]

Abstract

In the volcanic devastated site of Mt. Batur located in the north eastern part of Bali, Indonesia, the test construction with the soil protection sheet and the bag material for the prevention of soil erosion and the revegetation had been executed in December, 2012 and the effect has been verified by now. This time, the effect of the sheet on the soil microorganisms was investigated and the effect of the indigeneous arbuscular mycorrhizal fungi, which had been put into the bag material with 3 kinds of woody plant seeds, on the vegetation was verified. As a result, after 2 years and 8 months from the construction, it was showed that the soil microorganisms just under the sheet without the plant increased 2 to 2.4 times more than those in the bare site without the plant and furthermore, those just under the sheet with the plant increased 8.4 to 8.9 times more than those in the bare site without the plant. And it is considered that the cutting fragments in the length of about 5cm of the gramineous plant root existed near the construction site put into the bag material with the woody plant seeds contributed to the colonization of the arbuscular mycorrhizal fungi into the roots of seedlings from the seeds and the survival of the seedlings from the seeds as the inocula of the fungi.

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Introduction

• Menggambarkan keseluruhan isi artikel:

– Latar belakang – Metode

– Hasil dan Diskusi ringkas atau kesimpulan

• Dibuat singkat dan padat

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INTRODUCTION

Cultivation of edible mushrooms on sawdust-based substrate is steadily improving to effective methods. Especially, efficient techniques are useful for this cultivation method. Biological efficiency has been improved by optimizing various factors, such as substrate formula, strain type, culture maturity, water condition and other environmental conditions of the cultivation room. Electric power utility is now applied to various agricultural crops, especially in horticultural fields. Cultivation system has been improved with electricity utilization in the field of systematic house crops such as tomato, lettuce, strawberry and various kinds of flower. Effect of air ions on plants have experimented. Fruit body production was promoted by the electric impulse treatment on the logwood or sawdust substrate (Ohga et al, 2001) of L. edodes. We investigated here effect of the pulsed power on the fruit body formation in the sawdust-based substrate of 10 edible mushrooms.

MATERIALS AND METHODS

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Materials and Methods

• Menguraikan bahan yang digunakan

• Menguraikan metode yg digunakan

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MATERIALS AND METHODS Strains

Ten edible mushroom species used in this study, Lentinula edodes, Grifora frondosa, Pholiota nameko, Flammulina velutipes, Hypsizygus marmoreus, Pleurotus ostreatus, Pleurotus eryngii, Pleurotus abalones, Agrocybe cylindracea, Sparassis crispa. All of these strains were isolated from fruit bodies of commercial sources. Strains were maintained on a potato dextrose agar medium (Difco) at 40C. Especially, as for L. edodes four strains belonging to 3 diff erent fruiting types were examined in this work. These were KS-1, KS-2 (for year-round cultivation), KS-6 (for cultivation in autumn) and KS-12 (for cultivation in spring).

The substrate of L. edodes had three flushes in the fruiting stage.

First flush occurred spontaneously just after movement of the

substrates. Second and 3rd fl ushes were induced by soaking treatment of 16 hr (130-day substrate) and 20 hr (170-day substrate), respectively.

Culture and Growth Condition

Dst (lihat contoh jurnal – perhatikan cara menyajikan Materials and Methods nya

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Results and Discussion

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• Diskusikan kenapa angka itu terjadi, hasil itu menunjukkan apa, apa yang dapat ditarik

kesimpulan dari itu, dll

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Contoh Artikel Jurnal

• Cermati contoh artikel jurnal

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Acknowledgement

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- pemberi dana

– Kepada teman-teman yang membantu

– Kepada orang membantu mengkomunikasikan jurnalnya

– dll

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Reference

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sebagai pustaka (sebagian besar pustaka mesti berupa jurnal)

• Disertasi sebagai Pustaka

• Buku sebagai Pustaka

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Jenis-Jenis Artikel pada Jurnal

• Note

• Short Communication

• Review

• Full Paper

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Terimakasih