SPANISH ANANAS COMOSUS FRUIT
By
William 11405007
BACHELOR’S DEGREE in
FOOD TECHNOLOGY LIFE SCIENCES & TECHNOLOGY
SWISS GERMAN UNIVERSITY The Prominence Tower
Jalan Jalur Sutera Barat No. 15, Alam Sutera Tangerang, Banten 15143 - Indonesia
August 2018
Revision After Thesis Defense on July 26TH, 2018
STATEMENT BY THE AUTHOR
I hereby declare that this submission is my own work and to the best of my knowledge, it contains no material previously published or written by another person, nor material which to a substantial extent has been accepted for the award of any other degree or diploma at any educational institution, except where due acknowledgement is made in the thesis.
William
Student Date
Revision After Thesis Defense on July 26TH, 2018 Approved by:
Dr. Ir. Abdullah Muzi Marpaung, M.P.
Thesis Advisor Date
Della Rahmawati, S.Si, M.Si.
Thesis Co-Advisor Date
Dr. Dipl. -Ing. Samuel P. Kusumocahyo
ABSTRACT
STABILITY OF ANTHOCYANIN & ANTIOXIDANT ACTIVITY OF RTD BEVERAGE MADE FROM CLITORIA TERNATEA L. PETALS & RED SPANISH
ANANAS COMOSUS FRUIT
By William
Dr. Ir. Abdullah Muzi Marpaung, M.P, Advisor Della Rahmawati, S.Si, M.Si., Co-Advisor
SWISS GERMAN UNIVERSITY
This thesis focuses on the stability of anthocyanin, antioxidant activity, pH, color, and brix value in RTD product with various combinations. Clitoria ternatea L. (CTL) petals extract was proven to have higher stability with addition of Red Spanish Ananas comosus (RSAC) juice stored at cold temperature. Higher stability claimed due to lower degradation of anthocyanin, antioxidant, pH, color intensity, and °Brix compared to other treatments. CTL extract (4.8 mg/L) and RSAC juice (47.7 mg/mL) were used to formulate RTD beverages. The selected formulas for further analysis were; Formula CTL 45% and RSAC 55% (C45R55), CTL 50% and RSAC 50% (C50R50), and CTL 55% and RSAC 45% (C55R45). Formula C45R55 at cold temperature has better stability in antioxidant activity. It was also found that there is no significant different with C50R50, but significantly different with C55R45 and C100 in terms of antioxidant activity. Overall likeness analysis also resulted C45R55 has higher acceptance likeness score compared to other combinations. Thus, C45R55 has higher stability and overall likeness.
Keywords: Clitoria ternatea, Red Spanish Ananas comosus, anthocyanins, stability
© Copyright 2018 by William All rights reserved
DEDICATION
I dedicate this work to the future of Clitoria ternatea L. petals.
ACKNOWLEDGEMENTS
First of all, I would like to express my utmost gratitude to God for without His blessings and guidance, I would not be able to complete this thesis. I acknowledge that without the presence, help, support, and kindness of a lot people. Thus, I would like to thank those who have helped and supported me, either directly or indirectly, during the whole thesis and report writing experience.
Special thanks to my parents, for all of the support during my thesis research I would also like to thank Ir. Abdullah Muzi Marpaung, M.P. and Della Rahmawati, S.Si, M.Si, as my thesis advisor and Co-Advisor for their time and guidance during this research. I expand my thanks to Jennifer Sunardi for patiently listen to all my problems and try to solve them, and help me whenever.
I also want to thank Regina Michelle Salim and Gabriella Putri Gunawan for being helpful in my thesis book writing, to Justin Harimas in teaching me statistical analysis, and Calvin Latu who has been helpful in collecting samples. Last but not least, I also owe gratitude to Joshua Edward Wijaya and others to bring joy and happiness during this research. Thank you for the good memories and wish all the best for our future.
Hope this friendship will never end.
TABLE OF CONTENTS
Page
STATEMENT BY THE AUTHOR ... 2
ABSTRACT ... 3
DEDICATION ... 5
ACKNOWLEDGEMENTS ... 6
TABLE OF CONTENTS ... 7
LIST OF FIGURES ... 9
LIST OF TABLES ... 10
CHAPTER 1 - INTRODUCTION ... 11
1.1 Background ... 11
1.2 Research Problems ... 13
1.3 Research Objectives ... 13
1.4 Significance of Study ... 14
1.5 Research Questions ... 14
1.6 Hypothesis ... 14
CHAPTER 2 - LITERATURE REVIEW ... 15
2.1 Clitoria ternatea L. ... 15
2.2 Anthocyanin of Clitoria ternatea L. petals ... 16
2.3 Correlation between Anthocyanin and Antioxidant Activity ... 19
2.4 Red Spanish Ananas comosus ... 20
2.5 Extraction of Clitoria ternatea L. and Red Spanish Ananas comosus ... 21
2.6 Anti-Diabetic Activity of Clitoria ternatea L. ... 22
2.7 Pasteurization ... 23
CHAPTER 3 – RESEARCH METHODS ... 26
3.1 Venue and Time ... 26
3.2 Materials and Equipment ... 26
3.2.1 Materials ... 26
3.2.2 Equipment ... 26
3.2.3 Chemical Substances ... 26
3.3.2 Formulation and Sensory Evaluation ... 27
3.3.3 Stability Test ... 29
3.4 Analytical Procedure ... 29
CHAPTER 4 - RESULT AND DISCUSSION ... 32
4.1 Selection of Acceptable Combinations ... 32
4.2 Clitoria ternatea L. Combined with Red Spanish Ananas comosus Ready to Drink Beverage Properties ... 33
4.3 Stability Test ... 34
CHAPTER 5 - CONCLUSION AND RECOMMENDATIONS ... 46
5.1 Conclusion ... 46
5.2 Recommendations ... 46
REFERENCES ... 47
APPENDICES ... 56
CURRICULUM VITAE ………..94
